BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP18_F_K21
(909 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 37 0.003
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.006
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 36 0.008
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 34 0.032
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 33 0.074
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 32 0.13
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.17
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 3.7
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 26 6.4
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 26 6.4
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 26 8.5
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 26 8.5
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 37.1 bits (82), Expect = 0.003
Identities = 28/105 (26%), Positives = 31/105 (29%), Gaps = 3/105 (2%)
Frame = +3
Query: 603 TXRXTPHXVXNXHPXTPAXPPPXX--RXSCTLXPXXPXFXPXXXXXPXXPX-PXXPPXXX 773
T R P + P PPP R + L P P P P P
Sbjct: 372 TGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSL 431
Query: 774 PXXXPPPXPXGSXXXPPXLXXXPXXPSRXXPXPXXXPPPTXXPXP 908
P PP P G+ PP P P P P P P P
Sbjct: 432 PPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAP 476
Score = 33.9 bits (74), Expect = 0.032
Identities = 25/84 (29%), Positives = 26/84 (30%), Gaps = 2/84 (2%)
Frame = +3
Query: 663 PPXXRXSCTLXPXXPXFXPXXXXXPXXPXPXXPPXXXPXXXP--PPXPXGSXXXPPXLXX 836
PP S T P P P P PP P P PP P + PP
Sbjct: 404 PPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSL-PMGAPAAPPLPPSAPIAPPLPAG 462
Query: 837 XPXXPSRXXPXPXXXPPPTXXPXP 908
P P P PPP P P
Sbjct: 463 MPAAP--PLPPAAPAPPPAPAPAP 484
Score = 31.9 bits (69), Expect = 0.13
Identities = 16/55 (29%), Positives = 16/55 (29%)
Frame = +2
Query: 737 PXPXPPXXPPXXPXPXPSPXPXRVXXPSXXXXXXXXPPXPXXPPPXXPXXXNPPP 901
P PP PP P P P P P PP P PPP
Sbjct: 424 PPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPP 478
Score = 30.3 bits (65), Expect = 0.39
Identities = 17/57 (29%), Positives = 18/57 (31%), Gaps = 2/57 (3%)
Frame = +2
Query: 737 PXPXPPXXPPXXPXPXPSPXPXRVXXPSXXXXXXXXPPXPXXPP--PXXPXXXNPPP 901
P PP PP P P+ P P P P PP P P P P
Sbjct: 428 PPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAP 484
Score = 26.6 bits (56), Expect = 4.9
Identities = 18/58 (31%), Positives = 19/58 (32%), Gaps = 2/58 (3%)
Frame = +2
Query: 737 PXPXPPXXPPXXPXPXPSPXPXRVXXPSXXXXXXXXPPXPXXPPPXXPXXXNP--PPL 904
P P P P P +P R P PP PPP P P PPL
Sbjct: 352 PLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPP---APPPAIPGRSAPALPPL 406
Score = 26.2 bits (55), Expect = 6.4
Identities = 21/81 (25%), Positives = 24/81 (29%), Gaps = 1/81 (1%)
Frame = +3
Query: 654 AXPPPXXRXS-CTLXPXXPXFXPXXXXXPXXPXPXXPPXXXPXXXPPPXPXGSXXXPPXL 830
A PPP R + T P P P P P P PP S P +
Sbjct: 360 APPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPAL--PPLGNASRTSTPPV 417
Query: 831 XXXPXXPSRXXPXPXXXPPPT 893
P P P PP+
Sbjct: 418 PTPPSLPPSAPPSLPPSAPPS 438
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 36.3 bits (80), Expect = 0.006
Identities = 17/51 (33%), Positives = 19/51 (37%)
Frame = +2
Query: 749 PPXXPPXXPXPXPSPXPXRVXXPSXXXXXXXXPPXPXXPPPXXPXXXNPPP 901
PP PP P P+P P V P+ PP P P PPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 26.6 bits (56), Expect = 4.9
Identities = 14/51 (27%), Positives = 14/51 (27%)
Frame = +3
Query: 735 PXXPXPXXPPXXXPXXXPPPXPXGSXXXPPXLXXXPXXPSRXXPXPXXXPP 887
P P P P P P P PP P P P PP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 35.9 bits (79), Expect = 0.008
Identities = 26/90 (28%), Positives = 26/90 (28%), Gaps = 1/90 (1%)
Frame = +3
Query: 642 PXTPAXPPPXXRXSC-TLXPXXPXFXPXXXXXPXXPXPXXPPXXXPXXXPPPXPXGSXXX 818
P PA P P T P P P P P P PP P
Sbjct: 125 PSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAP-PIPSKAPPIPSSLPPPAQPAAPVKS 183
Query: 819 PPXLXXXPXXPSRXXPXPXXXPPPTXXPXP 908
PP P PS P P PPP P
Sbjct: 184 PPSA---PSLPSAVPPMPPKVPPPPLSQAP 210
Score = 28.3 bits (60), Expect = 1.6
Identities = 18/58 (31%), Positives = 19/58 (32%), Gaps = 2/58 (3%)
Frame = +2
Query: 737 PXPXPPXXPPXXPX--PXPSPXPXRVXXPSXXXXXXXXPPXPXXPPPXXPXXXNPPPL 904
P P P PP P PS P + P P PP P PPPL
Sbjct: 150 PPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVP-PPPL 206
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 33.9 bits (74), Expect = 0.032
Identities = 24/71 (33%), Positives = 24/71 (33%)
Frame = -3
Query: 907 GXGXXVGGGXXXGXGXXRXGXXGXXXXXGGXXXDPXGXGGGXXXGXXXGGXXGXGXXGXX 728
G G G G G G G G GG P G GGG G GG G G G
Sbjct: 203 GPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGG--PGGFGGGLGGFG-GGPG 259
Query: 727 XXXGXXXGXXG 695
G G G
Sbjct: 260 GFGGGPGGHGG 270
Score = 26.6 bits (56), Expect = 4.9
Identities = 18/57 (31%), Positives = 18/57 (31%), Gaps = 2/57 (3%)
Frame = -1
Query: 900 GGGLXXXGXXGGGXXGXGGXXXXXXXXXGXXTRXGXGEGXGXGXXG--GXXGGXGXG 736
GGG G GG G G G G G G G G GG G G
Sbjct: 187 GGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGG 243
Score = 25.8 bits (54), Expect = 8.5
Identities = 19/71 (26%), Positives = 19/71 (26%)
Frame = -3
Query: 907 GXGXXVGGGXXXGXGXXRXGXXGXXXXXGGXXXDPXGXGGGXXXGXXXGGXXGXGXXGXX 728
G G GG G G G G G GGG G G G G
Sbjct: 189 GFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFG 248
Query: 727 XXXGXXXGXXG 695
G G G
Sbjct: 249 GGLGGFGGGPG 259
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 32.7 bits (71), Expect = 0.074
Identities = 25/90 (27%), Positives = 28/90 (31%), Gaps = 4/90 (4%)
Frame = +3
Query: 651 PAXPPPXXRXSCTLXPXXPXFXPXXXXXPXXPXPXX--PPXXXPXXXPPPXPXGSXXXPP 824
P+ PP + S P P P P P PP P PP P + PP
Sbjct: 1136 PSGAPPVPKPSVAAPPV-----PAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPP 1190
Query: 825 XLXXX--PXXPSRXXPXPXXXPPPTXXPXP 908
P P P PP T P P
Sbjct: 1191 VPPPSEAPPVPKPSVGVPPVPPPSTAPPVP 1220
Score = 29.1 bits (62), Expect = 0.91
Identities = 20/79 (25%), Positives = 21/79 (26%)
Frame = +3
Query: 651 PAXPPPXXRXSCTLXPXXPXFXPXXXXXPXXPXPXXPPXXXPXXXPPPXPXGSXXXPPXL 830
P PP + S P P P P P PP P S PP
Sbjct: 1046 PTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPP-- 1103
Query: 831 XXXPXXPSRXXPXPXXXPP 887
P P P PP
Sbjct: 1104 VPKPSVAVPPVPAPSGAPP 1122
Score = 27.9 bits (59), Expect = 2.1
Identities = 21/88 (23%), Positives = 25/88 (28%), Gaps = 2/88 (2%)
Frame = +3
Query: 651 PAXPPPXXRXSCTLXPXXPXFXPXXXXXPXXPXPXXPPXXXPXXXPPPXPX--GSXXXPP 824
P+ PP + S P P P PP P PP P G+ P
Sbjct: 1088 PSGIPPVPKPSVAAPPVPK---PSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPK 1144
Query: 825 XLXXXPXXPSRXXPXPXXXPPPTXXPXP 908
P P+ P P P P
Sbjct: 1145 PSVAAPPVPAPSGAPPVPKPSVAAPPVP 1172
Score = 27.1 bits (57), Expect = 3.7
Identities = 14/50 (28%), Positives = 14/50 (28%)
Frame = +2
Query: 749 PPXXPPXXPXPXPSPXPXRVXXPSXXXXXXXXPPXPXXPPPXXPXXXNPP 898
PP PP P P P P P PP P PP
Sbjct: 1189 PPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPP 1238
Score = 26.6 bits (56), Expect = 4.9
Identities = 16/61 (26%), Positives = 17/61 (27%)
Frame = +3
Query: 705 PXFXPXXXXXPXXPXPXXPPXXXPXXXPPPXPXGSXXXPPXLXXXPXXPSRXXPXPXXXP 884
P P P P PP P PP P + PP PS P
Sbjct: 1199 PVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVSTPRSSVPS 1258
Query: 885 P 887
P
Sbjct: 1259 P 1259
Score = 26.2 bits (55), Expect = 6.4
Identities = 21/90 (23%), Positives = 21/90 (23%), Gaps = 1/90 (1%)
Frame = +3
Query: 642 PXTPAXPPPXXRXSCTLXPXXPXFXPXXXXXPXXPXPXXPPXXXPXXXPP-PXPXGSXXX 818
P PP S P P P P P PP P P G
Sbjct: 1035 PVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPV 1094
Query: 819 PPXLXXXPXXPSRXXPXPXXXPPPTXXPXP 908
P P P P P P P
Sbjct: 1095 PKPSVAAPPVPKPSVAVPPVPAPSGAPPVP 1124
Score = 25.8 bits (54), Expect = 8.5
Identities = 21/92 (22%), Positives = 27/92 (29%), Gaps = 3/92 (3%)
Frame = +3
Query: 642 PXTPAXPPPXXRXSCTLXPXXPXFXPXXXXXPXXPXPXXPPXXXPXXXPPPXPXGSXXXP 821
P + PP + + + P P P P PP PP P S P
Sbjct: 1172 PAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTA----PPVPTPSAGLP 1227
Query: 822 P---XLXXXPXXPSRXXPXPXXXPPPTXXPXP 908
P P P+ P P + P P
Sbjct: 1228 PVPVPTAKAPPVPAPSSEAPSVSTPRSSVPSP 1259
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 31.9 bits (69), Expect = 0.13
Identities = 19/55 (34%), Positives = 19/55 (34%), Gaps = 1/55 (1%)
Frame = -1
Query: 903 RGGGLXXXGXXGGGXXGXGGXXXXXXXXXGXXTRXG-XGEGXGXGXXGGXXGGXG 742
RGG G GG G GG R G G G G GG GG G
Sbjct: 11 RGGSRGGRGGFNGGRGGFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGGRG 65
Score = 27.1 bits (57), Expect = 3.7
Identities = 14/40 (35%), Positives = 14/40 (35%)
Frame = -3
Query: 907 GXGXXVGGGXXXGXGXXRXGXXGXXXXXGGXXXDPXGXGG 788
G G GG G G R G G GG G GG
Sbjct: 27 GFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGGRGG 66
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.5 bits (68), Expect = 0.17
Identities = 18/53 (33%), Positives = 21/53 (39%), Gaps = 2/53 (3%)
Frame = +2
Query: 752 PXXPPXXPXPXP-SPXPXRVXXPSXXXXXXXXPPXPXXPP-PXXPXXXNPPPL 904
P P P P S P ++ P+ PP P PP P P PPPL
Sbjct: 1683 PAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPL 1735
Score = 28.3 bits (60), Expect = 1.6
Identities = 15/45 (33%), Positives = 15/45 (33%)
Frame = +2
Query: 761 PPXXPXPXPSPXPXRVXXPSXXXXXXXXPPXPXXPPPXXPXXXNP 895
PP P P P P V P P P PPP P P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGP--PSAPPPPLPASSAP 1741
Score = 26.2 bits (55), Expect = 6.4
Identities = 16/58 (27%), Positives = 16/58 (27%)
Frame = +3
Query: 735 PXXPXPXXPPXXXPXXXPPPXPXGSXXXPPXLXXXPXXPSRXXPXPXXXPPPTXXPXP 908
P P PP PPP S PP P P P P P P
Sbjct: 1691 PVRPQSAAPPQMSAPTPPPPPM--SVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
Score = 25.8 bits (54), Expect = 8.5
Identities = 18/63 (28%), Positives = 18/63 (28%), Gaps = 2/63 (3%)
Frame = +3
Query: 642 PXTPAXPPPXXRXSCTLXPXXPXFXPXXXXXPXXPXPXXPPXXX--PXXXPPPXPXGSXX 815
P P PP R P P P P PP P PPP P S
Sbjct: 1683 PAHPVSTPPV-RPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
Query: 816 XPP 824
P
Sbjct: 1742 SVP 1744
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.1 bits (57), Expect = 3.7
Identities = 14/48 (29%), Positives = 14/48 (29%)
Frame = +2
Query: 737 PXPXPPXXPPXXPXPXPSPXPXRVXXPSXXXXXXXXPPXPXXPPPXXP 880
P P PP PP P P R PP PP P
Sbjct: 239 PPPPPPTLPPQSTNTSQLPMPSRNVNNLGSQVNIPPPPATPSQPPRPP 286
Score = 25.8 bits (54), Expect = 8.5
Identities = 11/31 (35%), Positives = 12/31 (38%)
Frame = +2
Query: 779 PXPSPXPXRVXXPSXXXXXXXXPPXPXXPPP 871
P PS P P+ PP P PPP
Sbjct: 167 PPPSFQPPSAAAPATSLPSDYNPPPPPPPPP 197
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 331
Score = 26.2 bits (55), Expect = 6.4
Identities = 14/45 (31%), Positives = 14/45 (31%)
Frame = +2
Query: 764 PXXPXPXPSPXPXRVXXPSXXXXXXXXPPXPXXPPPXXPXXXNPP 898
P P P P P P PP P P P P PP
Sbjct: 99 PEEPLPREPPLPNE---PVPEEPLPGEPPLPDEPVPEEPLPGEPP 140
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 26.2 bits (55), Expect = 6.4
Identities = 12/40 (30%), Positives = 13/40 (32%)
Frame = +1
Query: 760 PPXXPPAXXLPPXLXGXXPXXXXSPXXPXXXPXXXPXPXP 879
PP PP LPP + P P P P P
Sbjct: 522 PPMVPPGMALPPGMPAPFPGYPAVPAMPGIPGATAPPGAP 561
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 25.8 bits (54), Expect = 8.5
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = -2
Query: 401 ASTVPKPPWPCRSRLRALPWRLLAKALSCCSTDSEPSFQAL 279
AST+ K PWP + L +P + + CS+ +E ++ +
Sbjct: 38 ASTLEKEPWPASTALLVMPG---GRDMGYCSSFNETIYRKI 75
>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 25.8 bits (54), Expect = 8.5
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +2
Query: 359 DANGKAKEALEQSRXNIERTXEE 427
D+ KAK LEQ+R +ERT E
Sbjct: 403 DSIKKAKTELEQARIELERTQRE 425
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,216,489
Number of Sequences: 5004
Number of extensions: 35792
Number of successful extensions: 255
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 96
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 460503700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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