Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP18_F_C04
(888 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 168 8e-43
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 84 3e-17
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 80 5e-16
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 76 8e-15
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 47 3e-06
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 44 4e-05
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 38 0.002
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 29 0.88
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.2
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 8.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 168 bits (409), Expect = 8e-43
Identities = 77/97 (79%), Positives = 83/97 (85%)
Frame = +2
Query: 242 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHP 421
+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP
Sbjct: 43 IMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP 102
Query: 422 VLLTEAPLNPKGQQGRR*PXIMFETFNSPXHVRRIQA 532
LLTEAPLNPK + + I+FETFN+P IQA
Sbjct: 103 CLLTEAPLNPKSNREKM-TQIIFETFNAPAFYVAIQA 138
Score = 84.2 bits (199), Expect = 2e-17
Identities = 35/41 (85%), Positives = 40/41 (97%)
Frame = +1
Query: 118 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG 240
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHG 42
Score = 78.6 bits (185), Expect = 1e-15
Identities = 36/45 (80%), Positives = 38/45 (84%)
Frame = +3
Query: 507 PXMYVASRPXLSLYASGRTXGIVLDSGDGVSHTVPIYXGYALPHA 641
P YVA + LSLYASGRT GIVLDSGDGV+HTVPIY GYALPHA
Sbjct: 130 PAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHA 174
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 83.8 bits (198), Expect = 3e-17
Identities = 43/91 (47%), Positives = 59/91 (64%), Gaps = 2/91 (2%)
Frame = +2
Query: 242 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEH 418
VM QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + + +L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 419 PVLLTEAPL-NPKGQQGRR*PXIMFETFNSP 508
P+LLTE PL N + ++ + +ET N P
Sbjct: 107 PLLLTEPPLANIRNKE--KIAEYFYETLNVP 135
Score = 58.8 bits (136), Expect = 1e-09
Identities = 25/52 (48%), Positives = 37/52 (71%)
Frame = +3
Query: 507 PXMYVASRPXLSLYASGRTXGIVLDSGDGVSHTVPIYXGYALPHAHPPSGLG 662
P + + +P L+LYAS RT GIVL+ GDG++H+VPIY G+++P A +G
Sbjct: 135 PALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIG 186
Score = 52.8 bits (121), Expect = 6e-08
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +1
Query: 124 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 237
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 79.8 bits (188), Expect = 5e-16
Identities = 46/121 (38%), Positives = 64/121 (52%), Gaps = 4/121 (3%)
Frame = +2
Query: 182 ATTRPAPSSRPSWVALATRXVMVGMGQK---DSYVGDEAQSKRGI-LTLKYPIEHGIITN 349
A++ PA SS+PS++A + D ++G++A K +L YPI HG I N
Sbjct: 42 ASSGPAVSSKPSYMASKGSGHLSSKRATEDLDFFIGNDALKKASAGYSLDYPIRHGQIEN 101
Query: 350 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKGQQGRR*PXIMFETFNSPXHVRRIQ 529
WD ME+ W + + LR PE+H LLTE PLNP + IMFE+FN +Q
Sbjct: 102 WDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPP-ENRENTAEIMFESFNCAGLYIAVQ 160
Query: 530 A 532
A
Sbjct: 161 A 161
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +1
Query: 136 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 219
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
Score = 34.3 bits (75), Expect = 0.024
Identities = 12/22 (54%), Positives = 17/22 (77%)
Frame = +3
Query: 567 GIVLDSGDGVSHTVPIYXGYAL 632
G V+DSGDGV+H +P+ GY +
Sbjct: 181 GTVVDSGDGVTHIIPVAEGYVI 202
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 75.8 bits (178), Expect = 8e-15
Identities = 29/63 (46%), Positives = 48/63 (76%)
Frame = +2
Query: 263 KDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 442
KD VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 443 LNP 451
+NP
Sbjct: 110 MNP 112
Score = 53.6 bits (123), Expect = 4e-08
Identities = 23/42 (54%), Positives = 30/42 (71%)
Frame = +3
Query: 513 MYVASRPXLSLYASGRTXGIVLDSGDGVSHTVPIYXGYALPH 638
+YVA + LSLYA G + G+V+DSGDGV+H VP+Y L H
Sbjct: 132 VYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNH 173
Score = 46.8 bits (106), Expect = 4e-06
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +1
Query: 130 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 228
A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 47.2 bits (107), Expect = 3e-06
Identities = 25/86 (29%), Positives = 48/86 (55%), Gaps = 1/86 (1%)
Frame = +2
Query: 254 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 430
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 431 TEAPLNPKGQQGRR*PXIMFETFNSP 508
TE NP + ++FE +N+P
Sbjct: 121 TEPLANPTYVRSTM-TELLFELYNAP 145
Score = 37.5 bits (83), Expect = 0.003
Identities = 21/58 (36%), Positives = 34/58 (58%)
Frame = +1
Query: 58 AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 231
A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 43.6 bits (98), Expect = 4e-05
Identities = 21/67 (31%), Positives = 34/67 (50%)
Frame = +2
Query: 308 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKGQQGRR*PXIM 487
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP + + M
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKT-LETM 121
Query: 488 FETFNSP 508
FE+ P
Sbjct: 122 FESLRCP 128
Score = 41.5 bits (93), Expect = 2e-04
Identities = 23/60 (38%), Positives = 35/60 (58%), Gaps = 2/60 (3%)
Frame = +1
Query: 121 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGPERLLRG*RGPE 294
D+V+A+V+D GS + GF+G+D P+ V PS G + DGR +G E + + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYIYKSNPGME 64
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/42 (30%), Positives = 21/42 (50%)
Frame = +3
Query: 507 PXMYVASRPXLSLYASGRTXGIVLDSGDGVSHTVPIYXGYAL 632
P Y+A + + +ASG+ ++D G S IY G+ L
Sbjct: 128 PATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 37.9 bits (84), Expect = 0.002
Identities = 20/66 (30%), Positives = 34/66 (51%), Gaps = 2/66 (3%)
Frame = +2
Query: 263 KDSYVGDEAQSKRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 436
++ YV DE Q I + +K +GII +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 437 APLNPK 454
NP+
Sbjct: 109 PSWNPQ 114
Score = 34.3 bits (75), Expect = 0.024
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +1
Query: 121 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 222
+++ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 29.1 bits (62), Expect = 0.88
Identities = 14/39 (35%), Positives = 19/39 (48%)
Frame = +3
Query: 507 PXMYVASRPXLSLYASGRTXGIVLDSGDGVSHTVPIYXG 623
P +A P + LYA G G+V+D G + PI G
Sbjct: 176 PGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDG 214
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.2
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -3
Query: 346 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHHXPG 233
GDD+VLD V +D L L AH D H G
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAG 713
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 25.8 bits (54), Expect = 8.2
Identities = 15/41 (36%), Positives = 18/41 (43%), Gaps = 1/41 (2%)
Frame = +2
Query: 713 SRPPPPLTXXSXVXSRXPPAMFPLXFXXXXA-PXCPPPPLP 832
S PPPP + R PP PL + P PPP +P
Sbjct: 359 SAPPPPPPRSAPSTGRQPP---PLSSSRAVSNPPAPPPAIP 396
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,054,364
Number of Sequences: 5004
Number of extensions: 58873
Number of successful extensions: 194
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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