Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP18_F_C03
(880 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 86 7e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 42 2e-04
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 40 5e-04
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 40 6e-04
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 85.8 bits (203), Expect = 7e-18
Identities = 45/91 (49%), Positives = 51/91 (56%)
Frame = +3
Query: 384 AVRSGAYGXLFRPDXFVFGXSGAGNXWAKGXYXEGXXXXXXXXXXXRXXCXXXXCLQXFX 563
AV+SG +G LFRPD ++G SGAGN WAKG Y EG R LQ F
Sbjct: 75 AVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQ 134
Query: 564 LTHSLXGXXRFRXGXPSLISKIREEXPDRIM 656
LTHSL G G L+SKIREE PDR+M
Sbjct: 135 LTHSLGGGTGSGMG-TLLLSKIREEYPDRMM 164
Score = 62.1 bits (144), Expect = 1e-10
Identities = 31/71 (43%), Positives = 40/71 (56%)
Frame = +2
Query: 155 VXLXAGXCGXXXGAXFWEXXSXXXGIDPTGVYRGXSXLQLERXSVYYXXASVATAXXGGX 334
V + AG CG GA FW + G+D G+Y G S Q ER +VY+ A+ GG
Sbjct: 5 VHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAA------GGK 58
Query: 335 XVPRAILLDLE 367
VPRA+L+DLE
Sbjct: 59 YVPRAVLVDLE 69
Score = 40.3 bits (90), Expect = 4e-04
Identities = 20/51 (39%), Positives = 24/51 (47%)
Frame = +1
Query: 673 SPRPKYQXPXVXPYNPFSXSPXLVENTXXTYCXDNEGLYXIXYXXXKVPXP 825
+P PK V PYN LVEN+ T+C DNE L I K+ P
Sbjct: 170 APAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSP 220
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 41.5 bits (93), Expect = 2e-04
Identities = 22/71 (30%), Positives = 33/71 (46%)
Frame = +2
Query: 155 VXLXAGXCGXXXGAXFWEXXSXXXGIDPTGVYRGXSXLQLERXSVYYXXASVATAXXGGX 334
+ L AG CG G+ FW+ GI P G + ++R V++ +
Sbjct: 6 ITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSD------DTR 59
Query: 335 XVPRAILLDLE 367
+PRAIL+DLE
Sbjct: 60 YIPRAILIDLE 70
Score = 33.9 bits (74), Expect = 0.031
Identities = 23/92 (25%), Positives = 35/92 (38%), Gaps = 2/92 (2%)
Frame = +3
Query: 387 VRSGAYGXLFRPDXFVF--GXSGAGNXWAKGXYXEGXXXXXXXXXXXRXXCXXXXCLQXF 560
+ S YG L+ P+ + GAGN WA G Y L+ F
Sbjct: 77 ILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGF 135
Query: 561 XLTHSLXGXXRFRXGXPSLISKIREEXPDRIM 656
L HS+ G G L+ ++ + P +I+
Sbjct: 136 SLLHSIAGGTGSGLG-SFLLERLNDRYPKKII 166
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 39.9 bits (89), Expect = 5e-04
Identities = 22/66 (33%), Positives = 27/66 (40%)
Frame = +3
Query: 387 VRSGAYGXLFRPDXFVFGXSGAGNXWAKGXYXEGXXXXXXXXXXXRXXCXXXXCLQXFXL 566
VR+G Y LF P+ V G A N +A+G Y G R LQ F +
Sbjct: 82 VRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLV 141
Query: 567 THSLXG 584
HS G
Sbjct: 142 FHSFGG 147
Score = 30.7 bits (66), Expect = 0.29
Identities = 14/50 (28%), Positives = 21/50 (42%)
Frame = +1
Query: 676 PRPKYQXPXVXPYNPFSXSPXLVENTXXTYCXDNEGLYXIXYXXXKVPXP 825
P P+ V PYN + ++N+ T+ DNE Y I + P
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERP 226
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/71 (25%), Positives = 26/71 (36%)
Frame = +2
Query: 155 VXLXAGXCGXXXGAXFWEXXSXXXGIDPTGVYRGXSXLQLERXSVYYXXASVATAXXGGX 334
+ + G G G WE GI P G S + + + + G
Sbjct: 5 ISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSETGQGK 64
Query: 335 XVPRAILLDLE 367
VPR+I +DLE
Sbjct: 65 FVPRSIYVDLE 75
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 39.5 bits (88), Expect = 6e-04
Identities = 21/66 (31%), Positives = 27/66 (40%)
Frame = +3
Query: 387 VRSGAYGXLFRPDXFVFGXSGAGNXWAKGXYXEGXXXXXXXXXXXRXXCXXXXCLQXFXL 566
VR+G Y LF P+ + G A N +A+G Y G R LQ F +
Sbjct: 78 VRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLV 137
Query: 567 THSLXG 584
HS G
Sbjct: 138 FHSFGG 143
Score = 26.6 bits (56), Expect = 4.7
Identities = 13/50 (26%), Positives = 19/50 (38%)
Frame = +1
Query: 676 PRPKYQXPXVXPYNPFSXSPXLVENTXXTYCXDNEGLYXIXYXXXKVPXP 825
P P+ V PYN + ++ T+ DNE Y I + P
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERP 222
Score = 25.8 bits (54), Expect = 8.1
Identities = 18/71 (25%), Positives = 26/71 (36%)
Frame = +2
Query: 155 VXLXAGXCGXXXGAXFWEXXSXXXGIDPTGVYRGXSXLQLERXSVYYXXASVATAXXGGX 334
+ + G G G WE GI P G + Q ++ + G
Sbjct: 5 ISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGG----FSTFFSETGQGK 60
Query: 335 XVPRAILLDLE 367
VPR+I +DLE
Sbjct: 61 YVPRSIYVDLE 71
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,345,465
Number of Sequences: 5004
Number of extensions: 11507
Number of successful extensions: 23
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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