BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP18_F_B17
(948 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 31 0.31
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.55
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 1.3
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 28 2.2
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 2.9
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 8.9
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 30.7 bits (66), Expect = 0.31
Identities = 21/63 (33%), Positives = 21/63 (33%)
Frame = -1
Query: 558 GGGXXXPPPXXFXXGGGXXXGGGGXKKXXPPXXGXXPXGGXXPXGGGXXKKXXXXXGGAP 379
GGG PPP GG GG G G P GG GG GG
Sbjct: 194 GGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGP-GGFEGGPGGFGGGPGGFGGGLG 252
Query: 378 XFG 370
FG
Sbjct: 253 GFG 255
Score = 29.9 bits (64), Expect = 0.55
Identities = 24/82 (29%), Positives = 25/82 (30%), Gaps = 5/82 (6%)
Frame = -3
Query: 571 FXXXGGGXXXPPPXXFXXGGGXXXGG-----GGXXKKXPPPXGXXXXGGGASXGGGXXXK 407
F GGG PPP GG GG GG P G GG G G
Sbjct: 190 FGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGG 249
Query: 406 XXXXXWGGPXFWXXXRPNFXGP 341
GGP + GP
Sbjct: 250 GLGGFGGGPGGFGGGPGGHGGP 271
Score = 27.5 bits (58), Expect = 2.9
Identities = 26/87 (29%), Positives = 27/87 (31%), Gaps = 2/87 (2%)
Frame = -3
Query: 517 GGGXXXGGGGXXKKXPPPXGXXXXGGGASXGGGXXXKXXXXXWGGPXFWXXXRPNFXG-- 344
GGG GGG PPP G G G G GGP + F G
Sbjct: 187 GGGFGGFGGG--SGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGP 244
Query: 343 PFFXGXKKXFFXPPXXLGGEXKIXXGP 263
F G F P GG GP
Sbjct: 245 GGFGGGLGGFGGGPGGFGGGPGGHGGP 271
Score = 27.1 bits (57), Expect = 3.9
Identities = 22/73 (30%), Positives = 22/73 (30%), Gaps = 4/73 (5%)
Frame = -2
Query: 575 FXXXXXGGGXPPPPPXXXXG----GGXXXXGGGXXKKXPPPXGXXXPXGGXXPXGGGXXK 408
F G G PPP P G GG GG P G GG GG
Sbjct: 190 FGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGG 249
Query: 407 KXPXPXGGPPFLG 369
GGP G
Sbjct: 250 GLGGFGGGPGGFG 262
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.9 bits (64), Expect = 0.55
Identities = 18/64 (28%), Positives = 20/64 (31%), Gaps = 1/64 (1%)
Frame = -2
Query: 545 PPPPPXXXXGGGXXXXGGGXXKKXPPPXGXXXPXGGXXPXGGGXXKKXPXPXGGPPFL-G 369
PPPPP G PPP P G P + P PP + G
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPG 397
Query: 368 XSXP 357
S P
Sbjct: 398 RSAP 401
Score = 27.9 bits (59), Expect = 2.2
Identities = 18/66 (27%), Positives = 18/66 (27%), Gaps = 3/66 (4%)
Frame = -2
Query: 545 PPPPPXXXXGGGXXXXGGGXXKK---XPPPXGXXXPXGGXXPXGGGXXKKXPXPXGGPPF 375
PPPPP G G G PPP G G P P P
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPS 371
Query: 374 LGXSXP 357
G P
Sbjct: 372 TGRQPP 377
Score = 26.6 bits (56), Expect = 5.1
Identities = 15/46 (32%), Positives = 16/46 (34%)
Frame = +3
Query: 420 PPPXXAPPPXXXXPXGGGXFFXXPPPPXXXPPPXXXXXGGGXXXPP 557
PPP + P G G PPP PPP G PP
Sbjct: 313 PPPPPSRRNRGKPPIGNGSSNSSLPPP---PPPPRSNAAGSIPLPP 355
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 28.7 bits (61), Expect = 1.3
Identities = 19/60 (31%), Positives = 19/60 (31%)
Frame = +2
Query: 380 GAPPXXXXXFXXXPPPXGXXPPXGXXPXXGGXFFXXPPPPXXXPPPXXKXXGGGXXXPPP 559
GAPP P P P G P P PP PP K G PPP
Sbjct: 1157 GAPPVPKPSVAAPPVPA---PSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPP 1213
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 27.9 bits (59), Expect = 2.2
Identities = 17/49 (34%), Positives = 17/49 (34%), Gaps = 2/49 (4%)
Frame = +3
Query: 420 PPPXXAPPPXXXXPXGGGXFFXXPPPPX--XXPPPXXXXXGGGXXXPPP 560
PPP PPP P PPP PPP G PPP
Sbjct: 732 PPP---PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777
Score = 27.5 bits (58), Expect = 2.9
Identities = 17/50 (34%), Positives = 17/50 (34%), Gaps = 3/50 (6%)
Frame = +2
Query: 419 PPPX---GXXPPXGXXPXXGGXFFXXPPPPXXXPPPXXKXXGGGXXXPPP 559
PPP G PP P G PPP PPP G P P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGA----GPPPPPPPPPAVSAGGSRYYAPAP 797
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.5 bits (58), Expect = 2.9
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +3
Query: 420 PPPXXAPPPXXXXPXGGGXFFXXPPP 497
PPP PPP P G PPP
Sbjct: 1709 PPPMSVPPPPSAPPMPAGPPSAPPPP 1734
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 25.8 bits (54), Expect = 8.9
Identities = 11/31 (35%), Positives = 11/31 (35%)
Frame = +2
Query: 422 PPXGXXPPXGXXPXXGGXFFXXPPPPXXXPP 514
PP PP P PPPP PP
Sbjct: 167 PPPSFQPPSAAAPATSLPSDYNPPPPPPPPP 197
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,099,548
Number of Sequences: 5004
Number of extensions: 31359
Number of successful extensions: 153
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 96
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 483319012
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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