Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP17_F_P09
(881 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 150 2e-37
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 134 2e-32
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 132 6e-32
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 114 2e-26
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 102 8e-23
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 46 5e-06
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 150 bits (364), Expect = 2e-37
Identities = 81/147 (55%), Positives = 89/147 (60%)
Frame = +2
Query: 296 GXVXGIDLGXXYSCVGVYXNGRVXXXANDXGXRITPSYVAFTQDGXRLXGXAAKNQLTXN 475
G V GIDLG YSCV V NGRV AND G RITPSYVAFT+D RL G AAKNQ N
Sbjct: 35 GTVIGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTED-ERLVGEAAKNQAPSN 93
Query: 476 PENXVFDAKRLIGREWXXQTVXXDXXXXXXXXXXXXXXXXXXXXXXXGXXVFAPEXISAM 655
PEN +FD KRLIGR++ +T+ D F PE ISAM
Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153
Query: 656 VLTKMXXXAEAYLGKXVTHAVVTVPAY 736
+L+KM AEAYLGK VTHAVVTVPAY
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAY 180
Score = 43.6 bits (98), Expect = 4e-05
Identities = 20/29 (68%), Positives = 23/29 (79%)
Frame = +3
Query: 756 APKDAGTISGLXVXXLIXEPTAXAIAYGL 842
A KDAGTI+GL V ++ EPTA AIAYGL
Sbjct: 188 ATKDAGTIAGLNVIRIVNEPTAAAIAYGL 216
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 134 bits (324), Expect = 2e-32
Identities = 75/143 (52%), Positives = 81/143 (56%)
Frame = +2
Query: 308 GIDLGXXYSCVGVYXNGRVXXXANDXGXRITPSYVAFTQDGXRLXGXAAKNQLTXNPENX 487
GIDLG YSCVG + N RV AND G R TPSYVAFT D RL G AAKNQ+ NP N
Sbjct: 6 GIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHNT 64
Query: 488 VFDAKRLIGREWXXQTVXXDXXXXXXXXXXXXXXXXXXXXXXXGXXVFAPEXISAMVLTK 667
+FDAKRLIGR++ V D F PE IS+MVL K
Sbjct: 65 IFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVLMK 124
Query: 668 MXXXAEAYLGKXVTHAVVTVPAY 736
M AEAYLG VT AVVTVPAY
Sbjct: 125 MRETAEAYLGGKVTDAVVTVPAY 147
Score = 41.5 bits (93), Expect = 2e-04
Identities = 20/29 (68%), Positives = 22/29 (75%)
Frame = +3
Query: 756 APKDAGTISGLXVXXLIXEPTAXAIAYGL 842
A KDAG I+GL V +I EPTA AIAYGL
Sbjct: 155 ATKDAGLIAGLNVLRIINEPTAAAIAYGL 183
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 132 bits (319), Expect = 6e-32
Identities = 74/143 (51%), Positives = 80/143 (55%)
Frame = +2
Query: 308 GIDLGXXYSCVGVYXNGRVXXXANDXGXRITPSYVAFTQDGXRLXGXAAKNQLTXNPENX 487
GIDLG YSCVG + N RV AND G R TPSYVAFT D RL G AAKNQ+ NP N
Sbjct: 6 GIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHNT 64
Query: 488 VFDAKRLIGREWXXQTVXXDXXXXXXXXXXXXXXXXXXXXXXXGXXVFAPEXISAMVLTK 667
+FDAKRLIGR + V D F PE IS+MVL K
Sbjct: 65 IFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLK 124
Query: 668 MXXXAEAYLGKXVTHAVVTVPAY 736
M AEA+LG VT AVVTVPAY
Sbjct: 125 MRESAEAFLGGKVTDAVVTVPAY 147
Score = 41.5 bits (93), Expect = 2e-04
Identities = 20/29 (68%), Positives = 22/29 (75%)
Frame = +3
Query: 756 APKDAGTISGLXVXXLIXEPTAXAIAYGL 842
A KDAG I+GL V +I EPTA AIAYGL
Sbjct: 155 ATKDAGLIAGLNVLRIINEPTAAAIAYGL 183
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 114 bits (274), Expect = 2e-26
Identities = 66/143 (46%), Positives = 73/143 (51%)
Frame = +2
Query: 308 GIDLGXXYSCVGVYXNGRVXXXANDXGXRITPSYVAFTQDGXRLXGXAAKNQLTXNPENX 487
GIDLG YSCV V+ V ND G R TPS+VAFT+ RL G AAKNQ NP N
Sbjct: 10 GIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTET-ERLVGDAAKNQAAMNPRNT 68
Query: 488 VFDAKRLIGREWXXQTVXXDXXXXXXXXXXXXXXXXXXXXXXXGXXVFAPEXISAMVLTK 667
VFDAKRLIGR + D F + ISAMVLTK
Sbjct: 69 VFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVLTK 128
Query: 668 MXXXAEAYLGKXVTHAVVTVPAY 736
M +EA L K V AV+TVPAY
Sbjct: 129 MKEISEAKLNKRVEKAVITVPAY 151
Score = 42.3 bits (95), Expect = 9e-05
Identities = 20/30 (66%), Positives = 23/30 (76%)
Frame = +3
Query: 753 SAPKDAGTISGLXVXXLIXEPTAXAIAYGL 842
+A KDAG I+GL V +I EPTA AIAYGL
Sbjct: 158 AATKDAGAIAGLNVLRIINEPTAAAIAYGL 187
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 102 bits (244), Expect = 8e-23
Identities = 60/147 (40%), Positives = 70/147 (47%)
Frame = +2
Query: 296 GXVXGIDLGXXYSCVGVYXNGRVXXXANDXGXRITPSYVAFTQDGXRLXGXAAKNQLTXN 475
G V GIDLG SC+ + AN G R TPS VAFT+DG RL G +AK Q N
Sbjct: 50 GPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVIN 109
Query: 476 PENXVFDAKRLIGREWXXQTVXXDXXXXXXXXXXXXXXXXXXXXXXXGXXVFAPEXISAM 655
PEN F KRLIGR + V D ++P I
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGDAWLEAR---GKTYSPSQIGGF 166
Query: 656 VLTKMXXXAEAYLGKXVTHAVVTVPAY 736
+L+KM A YLGK V +AVVTVPAY
Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAY 193
Score = 37.1 bits (82), Expect = 0.003
Identities = 17/29 (58%), Positives = 21/29 (72%)
Frame = +3
Query: 756 APKDAGTISGLXVXXLIXEPTAXAIAYGL 842
A K AG I+GL V ++ EPTA A+AYGL
Sbjct: 201 ATKAAGAIAGLNVLRVVNEPTAAALAYGL 229
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 46.4 bits (105), Expect = 5e-06
Identities = 38/146 (26%), Positives = 57/146 (39%), Gaps = 1/146 (0%)
Frame = +2
Query: 302 VXGIDLGXXYSCVGVYXNGRVXXXANDXGXRITPSYVAFTQDGXRLXGXAAKNQLTXNPE 481
V GID G + + V N + N+ R TPS V++ + R G AAK+ N
Sbjct: 7 VVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERS-RFLGEAAKSAEASNFR 65
Query: 482 NXVFDAKRLIGREWXXQTV-XXDXXXXXXXXXXXXXXXXXXXXXXXGXXVFAPEXISAMV 658
N V KRL GR + + + F+ + A
Sbjct: 66 NTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAY 125
Query: 659 LTKMXXXAEAYLGKXVTHAVVTVPAY 736
TK+ AEA L V+ V+++PA+
Sbjct: 126 FTKIKAIAEAELIGSVSDVVISIPAW 151
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,782,657
Number of Sequences: 5004
Number of extensions: 19939
Number of successful extensions: 34
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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