Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP17_F_L02
(888 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 79 6e-16
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 79 6e-16
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 64 3e-11
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 42 2e-04
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 34 0.031
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 33 0.041
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 31 0.22
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 29 0.88
SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 26 8.2
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 79.4 bits (187), Expect = 6e-16
Identities = 46/92 (50%), Positives = 53/92 (57%)
Frame = +2
Query: 239 SLVSKXXXXXXXXXXXXRFTDTRKDEQXRCITIKSTXISMFFELEEKDLVFITNPDQXEK 418
SLV K RF DTR DEQ R +TIKST IS+F E+ + D+ + P
Sbjct: 38 SLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISLFAEMTDDDMKDMKEPADGTD 97
Query: 419 SEKGFLIXLIDSPGXVDFSSEVXXXLRVTDGA 514
FL+ LIDSPG VDFSSEV LRVTDGA
Sbjct: 98 ----FLVNLIDSPGHVDFSSEVTAALRVTDGA 125
Score = 60.9 bits (141), Expect = 2e-10
Identities = 33/83 (39%), Positives = 45/83 (54%)
Frame = +3
Query: 516 LXVVDCVSGVCVQTEXVLXQAIAEXIKPILFMXKMXRAXXXXXXXXXXXXQXFQXIVXNV 695
L VVD + GVCVQTE VL QA+ E I+P++ + K+ RA Q F +V +V
Sbjct: 126 LVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVESV 185
Query: 696 XVIIXXYXDDGGPMGEVXVXPXK 764
V+I Y D +G+ V P K
Sbjct: 186 NVVISTYYDK--VLGDCQVFPDK 206
Score = 38.7 bits (86), Expect = 0.001
Identities = 16/18 (88%), Positives = 18/18 (100%)
Frame = +1
Query: 181 LRNMSVLAHVDHGKSTLT 234
+RNMSV+AHVDHGKSTLT
Sbjct: 19 VRNMSVIAHVDHGKSTLT 36
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 79.4 bits (187), Expect = 6e-16
Identities = 46/92 (50%), Positives = 53/92 (57%)
Frame = +2
Query: 239 SLVSKXXXXXXXXXXXXRFTDTRKDEQXRCITIKSTXISMFFELEEKDLVFITNPDQXEK 418
SLV K RF DTR DEQ R +TIKST IS+F E+ + D+ + P
Sbjct: 38 SLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISLFAEMTDDDMKDMKEPADGTD 97
Query: 419 SEKGFLIXLIDSPGXVDFSSEVXXXLRVTDGA 514
FL+ LIDSPG VDFSSEV LRVTDGA
Sbjct: 98 ----FLVNLIDSPGHVDFSSEVTAALRVTDGA 125
Score = 60.9 bits (141), Expect = 2e-10
Identities = 33/83 (39%), Positives = 45/83 (54%)
Frame = +3
Query: 516 LXVVDCVSGVCVQTEXVLXQAIAEXIKPILFMXKMXRAXXXXXXXXXXXXQXFQXIVXNV 695
L VVD + GVCVQTE VL QA+ E I+P++ + K+ RA Q F +V +V
Sbjct: 126 LVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVESV 185
Query: 696 XVIIXXYXDDGGPMGEVXVXPXK 764
V+I Y D +G+ V P K
Sbjct: 186 NVVISTYYDK--VLGDCQVFPDK 206
Score = 38.7 bits (86), Expect = 0.001
Identities = 16/18 (88%), Positives = 18/18 (100%)
Frame = +1
Query: 181 LRNMSVLAHVDHGKSTLT 234
+RNMSV+AHVDHGKSTLT
Sbjct: 19 VRNMSVIAHVDHGKSTLT 36
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 63.7 bits (148), Expect = 3e-11
Identities = 38/75 (50%), Positives = 50/75 (66%)
Frame = +2
Query: 290 RFTDTRKDEQXRCITIKSTXISMFFELEEKDLVFITNPDQXEKSEKGFLIXLIDSPGXVD 469
RF D R+DE R IT+KS+ IS+FF++ I+ D+ ++ EK +LI LIDSPG VD
Sbjct: 55 RFLDFREDEITRGITMKSSAISLFFKV-------ISQNDE-KRVEKDYLINLIDSPGHVD 106
Query: 470 FSSEVXXXLRVTDGA 514
FSSEV R+ DGA
Sbjct: 107 FSSEVSSASRLCDGA 121
Score = 32.3 bits (70), Expect = 0.095
Identities = 12/17 (70%), Positives = 16/17 (94%)
Frame = +1
Query: 181 LRNMSVLAHVDHGKSTL 231
+RN ++LAHVDHGK+TL
Sbjct: 19 IRNFTLLAHVDHGKTTL 35
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 41.5 bits (93), Expect = 2e-04
Identities = 25/67 (37%), Positives = 35/67 (52%)
Frame = +2
Query: 314 EQXRCITIKSTXISMFFELEEKDLVFITNPDQXEKSEKGFLIXLIDSPGXVDFSSEVXXX 493
E+ + ITI+S +E + N Q EK + I +ID+PG +DF+ EV
Sbjct: 108 EREKGITIQSAATHCTWERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDFTIEVERA 165
Query: 494 LRVTDGA 514
LRV DGA
Sbjct: 166 LRVLDGA 172
Score = 27.5 bits (58), Expect = 2.7
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = +1
Query: 181 LRNMSVLAHVDHGKSTLTXLV 243
+RN+ + AH+D GK+T T V
Sbjct: 59 IRNIGISAHIDSGKTTFTERV 79
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 33.9 bits (74), Expect = 0.031
Identities = 23/74 (31%), Positives = 35/74 (47%)
Frame = +2
Query: 290 RFTDTRKDEQXRCITIKSTXISMFFELEEKDLVFITNPDQXEKSEKGFLIXLIDSPGXVD 469
R+TDT E+ R ++IKST +++ + K F ID+PG VD
Sbjct: 177 RYTDTHYLERERVMSIKSTPLTLAVS---------------DMKGKTFAFQCIDTPGHVD 221
Query: 470 FSSEVXXXLRVTDG 511
F EV + ++DG
Sbjct: 222 FVDEVAAPMAISDG 235
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 33.5 bits (73), Expect = 0.041
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = +1
Query: 181 LRNMSVLAHVDHGKSTLTXLV 243
+RN +V+AH+DHGKSTL+ +
Sbjct: 58 VRNWAVIAHIDHGKSTLSDCI 78
Score = 31.5 bits (68), Expect = 0.17
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = +2
Query: 425 KGFLIXLIDSPGXVDFSSEVXXXLRVTDG 511
+ +L+ LID+PG VDF +EV L +G
Sbjct: 122 QSYLLNLIDTPGHVDFRAEVMHSLAACEG 150
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 31.1 bits (67), Expect = 0.22
Identities = 11/27 (40%), Positives = 20/27 (74%)
Frame = +1
Query: 181 LRNMSVLAHVDHGKSTLTXLVGFQGRY 261
+RN+ ++AH+D GK+TLT + + G +
Sbjct: 28 IRNVGIIAHIDAGKTTLTEKMLYYGGF 54
Score = 30.7 bits (66), Expect = 0.29
Identities = 14/26 (53%), Positives = 17/26 (65%)
Frame = +2
Query: 437 IXLIDSPGXVDFSSEVXXXLRVTDGA 514
I LID+PG DF+ EV + V DGA
Sbjct: 95 INLIDTPGHADFTFEVERSVAVLDGA 120
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 29.1 bits (62), Expect = 0.88
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +1
Query: 187 NMSVLAHVDHGKSTLTXLV 243
N+ + HVDHGK+TLT +
Sbjct: 55 NIGTIGHVDHGKTTLTAAI 73
>SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting
endonuclease Cce1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 258
Score = 25.8 bits (54), Expect = 8.2
Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = -1
Query: 429 PFSLFSXWSGFVMNTK-SFSSSSKNIEMXVDLMVMQR 322
P S +S W+ V+NTK SFS ++M +L+ Q+
Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELIDGQK 203
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,952,741
Number of Sequences: 5004
Number of extensions: 24287
Number of successful extensions: 71
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 59
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 68
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -