Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP17_F_C01
(857 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P31150 Cluster: Rab GDP dissociation inhibitor alpha; n... 97 5e-19
UniRef50_Q5KEK9 Cluster: RAB GDP-dissociation inhibitor, putativ... 77 6e-13
UniRef50_A6SLT8 Cluster: Secretory pathway Rab GDP dissociation ... 70 7e-11
UniRef50_Q00SF8 Cluster: GDP dissociation inhibitor-common tobac... 69 1e-10
UniRef50_UPI00015552EE Cluster: PREDICTED: similar to Rab GDP di... 64 3e-09
UniRef50_A0CDI7 Cluster: Chromosome undetermined scaffold_17, wh... 62 2e-08
UniRef50_Q9GU77 Cluster: GDI; n=2; Giardia intestinalis|Rep: GDI... 61 3e-08
UniRef50_A2G9W5 Cluster: GDP dissociation inhibitor family prote... 57 5e-07
UniRef50_Q4E3K7 Cluster: RAB GDP dissociation inhibitor alpha, p... 50 1e-04
UniRef50_Q6AZH3 Cluster: CHML protein; n=4; Tetrapoda|Rep: CHML ... 38 0.25
UniRef50_A7R9G5 Cluster: Chromosome undetermined scaffold_3816, ... 38 0.32
UniRef50_A2ZN29 Cluster: Putative uncharacterized protein; n=1; ... 35 2.3
>UniRef50_P31150 Cluster: Rab GDP dissociation inhibitor alpha;
n=188; Eukaryota|Rep: Rab GDP dissociation inhibitor
alpha - Homo sapiens (Human)
Length = 447
Score = 97.1 bits (231), Expect = 5e-19
Identities = 53/129 (41%), Positives = 65/129 (50%), Gaps = 1/129 (0%)
Frame = +2
Query: 281 LXXLFAKFXA-PAPDXTYGRGRXWXVXLIPKFXXXXXXXXXXXIHXGVXRYLEFKSXEGX 457
L L+ +F P + GRGR W V LIPKF ++ V RYL+FK EG
Sbjct: 49 LEELYKRFQLLEGPPESMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVVEGS 108
Query: 458 XVYXGGXISKVPVDXKXALASDLMGXFEXXRFRXFLIYVXXFQXXXAXXWXXFDPSTAXX 637
VY GG I KVP ALAS+LMG FE RFR FL++V F + DP T
Sbjct: 109 FVYKGGKIYKVPSTETEALASNLMGMFEKRRFRKFLVFVANFDENDPKTFEGVDPQTTSM 168
Query: 638 QSLYXTFGL 664
+ +Y F L
Sbjct: 169 RDVYRKFDL 177
Score = 52.4 bits (120), Expect = 1e-05
Identities = 26/48 (54%), Positives = 29/48 (60%)
Frame = +1
Query: 136 MXXXYXVXVLGXGLKXCXLSGXLSVSGXXVLXXDRXXXYGGXSASXTP 279
M Y V VLG GL C LSG +SV+G VL DR YGG S+S TP
Sbjct: 1 MDEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESSSITP 48
Score = 39.5 bits (88), Expect = 0.11
Identities = 23/54 (42%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +1
Query: 682 FXGXALALYLDXXYLXXPAIRPFXXSVVF*-SLXKYGXSPXLXPLXGLXAPPXG 840
F G ALALY YL P + ++ SL +YG SP L PL GL P G
Sbjct: 184 FTGHALALYRTDDYLDQPCLETVNRIKLYSESLARYGKSPYLYPLYGLGELPQG 237
>UniRef50_Q5KEK9 Cluster: RAB GDP-dissociation inhibitor, putative;
n=13; Eukaryota|Rep: RAB GDP-dissociation inhibitor,
putative - Cryptococcus neoformans (Filobasidiella
neoformans)
Length = 551
Score = 77.0 bits (181), Expect = 6e-13
Identities = 47/131 (35%), Positives = 59/131 (45%), Gaps = 2/131 (1%)
Frame = +2
Query: 281 LXXLFAKFXAPAP--DXTYGRGRXWXVXLIPKFXXXXXXXXXXXIHXGVXRYLEFKSXEG 454
L L+ KF P + GR R + V LIPKF +H V RYLEFK G
Sbjct: 147 LTQLYQKFRGTPPPENLQLGRDRDYAVDLIPKFILSSGELTRMLVHTDVTRYLEFKVIAG 206
Query: 455 XXVYXGGXISKVPVDXKXALASDLMGXFEXXRFRXFLIYVXXFQXXXAXXWXXFDPSTAX 634
VY G ISKVP A+ S LMG FE R R F Y+ ++ D +
Sbjct: 207 SYVYRDGKISKVPSTEMEAVKSPLMGLFEKRRARNFFQYLQNWKEEDPATHQGLDINKCP 266
Query: 635 XQSLYXTFGLD 667
+ +Y FGL+
Sbjct: 267 MKDVYTKFGLE 277
Score = 49.6 bits (113), Expect = 1e-04
Identities = 26/45 (57%), Positives = 26/45 (57%)
Frame = +1
Query: 136 MXXXYXVXVLGXGLKXCXLSGXLSVSGXXVLXXDRXXXYGGXSAS 270
M Y V VLG GL C LSG LSV G VL DR YGG SAS
Sbjct: 100 MDEEYDVIVLGTGLTECILSGLLSVDGQKVLHMDRNDYYGGDSAS 144
>UniRef50_A6SLT8 Cluster: Secretory pathway Rab GDP dissociation
inhibitor; n=2; Sclerotiniaceae|Rep: Secretory pathway
Rab GDP dissociation inhibitor - Botryotinia fuckeliana
B05.10
Length = 471
Score = 70.1 bits (164), Expect = 7e-11
Identities = 40/121 (33%), Positives = 53/121 (43%), Gaps = 4/121 (3%)
Frame = +2
Query: 317 PDXTYGRGRXWXVXLIPKFXXXXXXXXXXXIHXGVXRYLEFKSXEGXXVYXG----GXIS 484
P YGR W + L+PK + V RYLEF+S G V G ++
Sbjct: 67 PWKKYGRANDWNIDLVPKLLMSSGELTNILVSTDVTRYLEFRSVAGSYVQQGTGPKAMVA 126
Query: 485 KVPVDXKXALASDLMGXFEXXRFRXFLIYVXXFQXXXAXXWXXFDPSTAXXQSLYXTFGL 664
KVP D AL S LMG FE R + FL ++ F + ST + +Y FGL
Sbjct: 127 KVPSDAGEALRSSLMGIFEKRRMKSFLEWIGTFDAADPATHKGLNMSTCTMKDIYDKFGL 186
Query: 665 D 667
+
Sbjct: 187 E 187
Score = 47.6 bits (108), Expect = 4e-04
Identities = 24/41 (58%), Positives = 25/41 (60%)
Frame = +1
Query: 148 YXVXVLGXGLKXCXLSGXLSVSGXXVLXXDRXXXYGGXSAS 270
Y V VLG GL C LSG LSV G VL DR YGG +AS
Sbjct: 8 YDVVVLGTGLTECVLSGVLSVKGQKVLHIDRNDHYGGEAAS 48
>UniRef50_Q00SF8 Cluster: GDP dissociation inhibitor-common tobacco;
n=1; Ostreococcus tauri|Rep: GDP dissociation
inhibitor-common tobacco - Ostreococcus tauri
Length = 432
Score = 69.3 bits (162), Expect = 1e-10
Identities = 38/113 (33%), Positives = 55/113 (48%), Gaps = 1/113 (0%)
Frame = +2
Query: 329 YGRGRXWXVXLIPKFXXXXXXXXXXXIHXGVXRYLEFKSXEGXXVYX-GGXISKVPVDXK 505
YGR + + + LIPK+ + GV +Y++F++ +G V GG I KVP + K
Sbjct: 71 YGRYQDYNIDLIPKYIMGNGLLTKVLVKTGVHQYIQFRAGDGSFVVGKGGKIHKVPANDK 130
Query: 506 XALASDLMGXFEXXRFRXFLIYVXXFQXXXAXXWXXFDPSTAXXQSLYXTFGL 664
AL S LMG FE R R F ++V F ++ + LY FGL
Sbjct: 131 EALRSSLMGMFEKLRARSFFVFVQNFVETDPSTHGGYNLHRMPARELYEKFGL 183
Score = 41.9 bits (94), Expect = 0.020
Identities = 24/46 (52%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +1
Query: 136 MXXXYXVXVLGXGLKXCXLSGXLS-VSGXXVLXXDRXXXYGGXSAS 270
M Y V VLG GLK C ++G LS V VL DR YGG SAS
Sbjct: 1 MDQTYDVVVLGTGLKECLVAGVLSAVERMKVLHVDRNDYYGGESAS 46
>UniRef50_UPI00015552EE Cluster: PREDICTED: similar to Rab GDP
dissociation inhibitor alpha (Rab GDI alpha) (Guanosine
diphosphate dissociation inhibitor 1) (GDI-1), partial;
n=1; Ornithorhynchus anatinus|Rep: PREDICTED: similar to
Rab GDP dissociation inhibitor alpha (Rab GDI alpha)
(Guanosine diphosphate dissociation inhibitor 1)
(GDI-1), partial - Ornithorhynchus anatinus
Length = 562
Score = 64.5 bits (150), Expect = 3e-09
Identities = 35/78 (44%), Positives = 41/78 (52%), Gaps = 1/78 (1%)
Frame = +2
Query: 290 LFAKFXAP-APDXTYGRGRXWXVXLIPKFXXXXXXXXXXXIHXGVXRYLEFKSXEGXXVY 466
L+ +F P P + GRGR W V LIPKF ++ V RYL+FK EG VY
Sbjct: 214 LYKRFELPEGPPESMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVVEGSFVY 273
Query: 467 XGGXISKVPVDXKXALAS 520
GG I KVP ALAS
Sbjct: 274 KGGKIYKVPSTETEALAS 291
>UniRef50_A0CDI7 Cluster: Chromosome undetermined scaffold_17, whole
genome shotgun sequence; n=2; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_17,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 469
Score = 61.7 bits (143), Expect = 2e-08
Identities = 36/95 (37%), Positives = 46/95 (48%), Gaps = 10/95 (10%)
Frame = +2
Query: 332 GRGRXWXVXLIPKFXXXXXXXXXXXIHXGVXRYLEFKSXEGXXVYX----------GGXI 481
G+ R W + LIPKF + V RYLE+K+ +G V+ GG I
Sbjct: 72 GQNRDWNIDLIPKFVMANGQLVKILLKTKVARYLEWKAIDGTYVFQMKEPGLFSKGGGKI 131
Query: 482 SKVPVDXKXALASDLMGXFEXXRFRXFLIYVXXFQ 586
KVP AL SDLMG FE R + FL YV ++
Sbjct: 132 EKVPATASEALKSDLMGMFEKRRCQKFLAYVSNYE 166
Score = 41.5 bits (93), Expect = 0.026
Identities = 21/41 (51%), Positives = 22/41 (53%)
Frame = +1
Query: 148 YXVXVLGXGLKXCXLSGXLSVSGXXVLXXDRXXXYGGXSAS 270
Y V V G GL C LSG LS+ G V DR YGG AS
Sbjct: 11 YDVVVCGTGLIECILSGLLSMEGKRVFHMDRNPYYGGEGAS 51
Score = 37.1 bits (82), Expect = 0.57
Identities = 19/54 (35%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
Frame = +1
Query: 682 FXGXALALYLDXXYLXXPAIRPFXXSVVF*-SLXKYGXSPXLXPLXGLXAPPXG 840
F G A+AL+ + +L PAI+ ++ S+ +YG SP + P+ GL P G
Sbjct: 199 FIGHAVALFSNDLFLDKPAIQTIEKIKLYMDSIGRYGDSPFIYPIYGLGGIPEG 252
>UniRef50_Q9GU77 Cluster: GDI; n=2; Giardia intestinalis|Rep: GDI -
Giardia lamblia (Giardia intestinalis)
Length = 476
Score = 61.3 bits (142), Expect = 3e-08
Identities = 35/105 (33%), Positives = 44/105 (41%)
Frame = +2
Query: 272 LPRLXXLFAKFXAPAPDXTYGRGRXWXVXLIPKFXXXXXXXXXXXIHXGVXRYLEFKSXE 451
L +L F + + P +G+ W + LIPKF H YLEF
Sbjct: 51 LSQLYSFFGESLSSIP-AEFGKDNEWSIDLIPKFILSSGDLFYMLRHVDCLHYLEFGRVA 109
Query: 452 GXXVYXGGXISKVPVDXKXALASDLMGXFEXXRFRXFLIYVXXFQ 586
G VY G I +VP K AL S LMG FE R Y+ F+
Sbjct: 110 GAFVYNNGVIHRVPATTKQALDSKLMGLFEKKRMANLFEYITSFE 154
Score = 37.1 bits (82), Expect = 0.57
Identities = 20/41 (48%), Positives = 22/41 (53%)
Frame = +1
Query: 148 YXVXVLGXGLKXCXLSGXLSVSGXXVLXXDRXXXYGGXSAS 270
+ VLG GLK +S LSV G VL DR YGG AS
Sbjct: 8 FDAIVLGTGLKEGIVSALLSVHGRKVLHIDRNDFYGGDCAS 48
>UniRef50_A2G9W5 Cluster: GDP dissociation inhibitor family protein;
n=1; Trichomonas vaginalis G3|Rep: GDP dissociation
inhibitor family protein - Trichomonas vaginalis G3
Length = 439
Score = 57.2 bits (132), Expect = 5e-07
Identities = 30/84 (35%), Positives = 39/84 (46%)
Frame = +2
Query: 332 GRGRXWXVXLIPKFXXXXXXXXXXXIHXGVXRYLEFKSXEGXXVYXGGXISKVPVDXKXA 511
G R W + LIPKF IH V L F+ G V G + KVP + K A
Sbjct: 65 GPNREWNIDLIPKFIMADGKLVKALIHTKVNESLNFQFIAGSYVLSNGKVDKVPSNAKEA 124
Query: 512 LASDLMGXFEXXRFRXFLIYVXXF 583
LA+ L+G FE + FL +V +
Sbjct: 125 LATSLVGFFEKRHLKNFLEFVADY 148
Score = 45.2 bits (102), Expect = 0.002
Identities = 23/45 (51%), Positives = 24/45 (53%)
Frame = +1
Query: 136 MXXXYXVXVLGXGLKXCXLSGXLSVSGXXVLXXDRXXXYGGXSAS 270
M Y V G G K C LSG LSV+G VL DR YGG AS
Sbjct: 1 MEEKYDVIACGTGFKECLLSGLLSVAGKHVLHVDRNDFYGGECAS 45
>UniRef50_Q4E3K7 Cluster: RAB GDP dissociation inhibitor alpha,
putative; n=4; Trypanosomatidae|Rep: RAB GDP
dissociation inhibitor alpha, putative - Trypanosoma
cruzi
Length = 445
Score = 49.6 bits (113), Expect = 1e-04
Identities = 35/129 (27%), Positives = 49/129 (37%), Gaps = 1/129 (0%)
Frame = +2
Query: 281 LXXLFAKFXAPAPDXTYGRGRXWXVXLIPKFXXXXXXXXXXXIHXGVXRY-LEFKSXEGX 457
L L+ KF AP + GR + V LIPK + RY +EF +
Sbjct: 48 LEQLYQKFNKGAPPASLGRSHLYNVDLIPKVLMCAGELVKILRCTVIDRYNMEFMLIDNS 107
Query: 458 XVYXGGXISKVPVDXKXALASDLMGXFEXXRFRXFLIYVXXFQXXXAXXWXXFDPSTAXX 637
V G I+KVP AL S LMG FE + ++ + ++
Sbjct: 108 FVIKDGKIAKVPATEAEALMSPLMGFFEKRKAAKLFQFMGNYDPKNPKTHKNYNLHAMTM 167
Query: 638 QSLYXTFGL 664
LY FG+
Sbjct: 168 AQLYKEFGI 176
Score = 46.4 bits (105), Expect = 0.001
Identities = 24/45 (53%), Positives = 25/45 (55%)
Frame = +1
Query: 136 MXXXYXVXVLGXGLKXCXLSGXLSVSGXXVLXXDRXXXYGGXSAS 270
M Y V G GL C LSG LSV+G VL DR YGG SAS
Sbjct: 1 MEESYDAVVCGTGLTECVLSGLLSVNGYKVLHVDRNPYYGGESAS 45
>UniRef50_Q6AZH3 Cluster: CHML protein; n=4; Tetrapoda|Rep: CHML
protein - Xenopus laevis (African clawed frog)
Length = 643
Score = 38.3 bits (85), Expect = 0.25
Identities = 22/83 (26%), Positives = 32/83 (38%)
Frame = +2
Query: 338 GRXWXVXLIPKFXXXXXXXXXXXIHXGVXRYLEFKSXEGXXVYXGGXISKVPVDXKXALA 517
GR + + L+ KF I V RY EFK+ Y G I +VP A
Sbjct: 213 GRRFNIDLVAKFLYSRGLLIELLIKSNVSRYTEFKNVTRILTYHDGKIEQVPCSRADVFA 272
Query: 518 SDLMGXFEXXRFRXFLIYVXXFQ 586
S + E FL++ ++
Sbjct: 273 SKQLSMVEKRILMKFLMHYVDYE 295
>UniRef50_A7R9G5 Cluster: Chromosome undetermined scaffold_3816,
whole genome shotgun sequence; n=2; Eukaryota|Rep:
Chromosome undetermined scaffold_3816, whole genome
shotgun sequence - Vitis vinifera (Grape)
Length = 36
Score = 37.9 bits (84), Expect = 0.32
Identities = 19/31 (61%), Positives = 20/31 (64%)
Frame = +1
Query: 136 MXXXYXVXVLGXGLKXCXLSGXLSVSGXXVL 228
M Y V VLG GLK C LSG LSV+G VL
Sbjct: 1 MDEEYDVIVLGTGLKECILSGILSVNGLKVL 31
>UniRef50_A2ZN29 Cluster: Putative uncharacterized protein; n=1;
Oryza sativa (indica cultivar-group)|Rep: Putative
uncharacterized protein - Oryza sativa subsp. indica
(Rice)
Length = 153
Score = 35.1 bits (77), Expect = 2.3
Identities = 18/31 (58%), Positives = 18/31 (58%)
Frame = +1
Query: 136 MXXXYXVXVLGXGLKXCXLSGXLSVSGXXVL 228
M Y V VLG GL C LSG LSV G VL
Sbjct: 1 MDEEYDVIVLGTGLMECILSGLLSVDGLKVL 31
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 427,338,330
Number of Sequences: 1657284
Number of extensions: 4140624
Number of successful extensions: 3871
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 3798
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3867
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 75833093035
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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