BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP17_F_B20
(928 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.057
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 33 0.075
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 32 0.100
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.13
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 31 0.23
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.93
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.8
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 27 3.8
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 5.0
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 5.0
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 6.6
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 33.1 bits (72), Expect = 0.057
Identities = 28/114 (24%), Positives = 31/114 (27%), Gaps = 5/114 (4%)
Frame = -3
Query: 620 PXXPPP---TXXXPXPPPXXPXXXPXFXXXXXXXPKXXXPPPPXXXXX--FXPXQKXXPP 456
P PPP T P P P K PPP +K PP
Sbjct: 254 PPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPPP 313
Query: 455 PXPPXKXKKNXPPXXPXFSPPXXPXTXXXXXXXXXXXXXXXXXXXLSXXPPPPP 294
P PP + + PP S P S PPPPP
Sbjct: 314 PPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGR-SAPPPPPP 366
Score = 29.1 bits (62), Expect = 0.93
Identities = 20/70 (28%), Positives = 21/70 (30%), Gaps = 5/70 (7%)
Frame = -1
Query: 589 PXPPPXXPXXXXXFXXXXKXXPKXXXPP---PPXXPXPXPPXKXXXPPPXPXKXKKKTPP 419
P PPP P + PP PP P PP PP PP
Sbjct: 389 PAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPP 448
Query: 418 L--XXPXFPP 395
L P PP
Sbjct: 449 LPPSAPIAPP 458
Score = 28.7 bits (61), Expect = 1.2
Identities = 16/61 (26%), Positives = 17/61 (27%)
Frame = -1
Query: 580 PPXXPXXXXXFXXXXKXXPKXXXPPPPXXPXPXPPXKXXXPPPXPXKXKKKTPPLXXPXF 401
PP P P+ PPP P P PPP PP P
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPR-SAPSTGRQPPPLSSSRAVSNPPAPPPAI 395
Query: 400 P 398
P
Sbjct: 396 P 396
Score = 27.9 bits (59), Expect = 2.1
Identities = 18/73 (24%), Positives = 20/73 (27%)
Frame = -1
Query: 610 PPXXXXXPXPPPXXPXXXXXFXXXXKXXPKXXXPPPPXXPXPXPPXKXXXPPPXPXKXKK 431
PP P PPP PP P P PP +
Sbjct: 354 PPQGRSAPPPPPPRSAPSTG-RQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRT 412
Query: 430 KTPPLXXPXFPPP 392
TPP+ P PP
Sbjct: 413 STPPVPTPPSLPP 425
Score = 27.5 bits (58), Expect = 2.8
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = -2
Query: 306 PSPPTXXXFFTPPXPPLXXXPPPPPP 229
P PP + P PP PPPPP
Sbjct: 340 PPPPRSNAAGSIPLPPQGRSAPPPPP 365
Score = 26.6 bits (56), Expect = 5.0
Identities = 29/138 (21%), Positives = 32/138 (23%), Gaps = 2/138 (1%)
Frame = -2
Query: 639 PXSXXXXXXPPPXXXXXXXPPXXXXXXPXFFXXXXXXPQKXXXPPPPXXXXLXPXXKXXX 460
P S P P PP P PP P
Sbjct: 343 PRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPA 402
Query: 459 PPPSPXKXKKKXPPXSXLXFPPPXTXHKXPXTXXXXXXXXXXXXPXSFXFXP-SPPTXXX 283
PP + PP PP P + P P +PP
Sbjct: 403 LPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAG 462
Query: 282 F-FTPPXPPLXXXPPPPP 232
PP PP PPP P
Sbjct: 463 MPAAPPLPPAAPAPPPAP 480
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 32.7 bits (71), Expect = 0.075
Identities = 16/37 (43%), Positives = 17/37 (45%), Gaps = 2/37 (5%)
Frame = -1
Query: 526 PKXXXPPPPXXPXPXPPXKXXXPPP--XPXKXKKKTP 422
P PPPP P PP + PPP K K KTP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPPGYVKKRKNKTP 41
Score = 30.7 bits (66), Expect = 0.30
Identities = 14/37 (37%), Positives = 15/37 (40%)
Frame = -3
Query: 527 PKXXXPPPPXXXXXFXPXQKXXPPPXPPXKXKKNXPP 417
P PPPP P Q PPP K +KN P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPPGYVKKRKNKTP 41
Score = 29.1 bits (62), Expect = 0.93
Identities = 13/26 (50%), Positives = 13/26 (50%)
Frame = -2
Query: 306 PSPPTXXXFFTPPXPPLXXXPPPPPP 229
P PP F PP P PPPPPP
Sbjct: 9 PPPPPPPPGFEPPSQP----PPPPPP 30
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 32.3 bits (70), Expect = 0.100
Identities = 24/77 (31%), Positives = 25/77 (32%)
Frame = +1
Query: 409 GXXGGXFFFXFXGGXGGGXXFXXGXKXXXXWGGGGXXFLGXXXXXXKKXGXXXGXXGGGX 588
G GG F F GG GG G +GG G G G G G G
Sbjct: 184 GHNGGGFG-GFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGG 242
Query: 589 GXXXVGGGXXGXXXXXG 639
G GGG G G
Sbjct: 243 GPGGFGGGLGGFGGGPG 259
Score = 29.9 bits (64), Expect = 0.53
Identities = 19/63 (30%), Positives = 19/63 (30%)
Frame = +3
Query: 423 GVFFXFXXGXGGGXXFLXGGKGXGXXGGGGXXXFGXXXXXXXKXWXXXGXXGGGXGXXXX 602
G F GGG GG G G GG FG G GG G
Sbjct: 177 GNLFHHRGHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGG 236
Query: 603 XGG 611
GG
Sbjct: 237 PGG 239
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 31.9 bits (69), Expect = 0.13
Identities = 18/56 (32%), Positives = 18/56 (32%), Gaps = 1/56 (1%)
Frame = -1
Query: 583 PPPXXPXXXXXFXXXXKXXPKXXXPPPPXXPXPXP-PXKXXXPPPXPXKXKKKTPP 419
P P P K K PPPP P P P PPP P PP
Sbjct: 710 PSPLLPDVSDTVEEQQKLLLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPP 765
Score = 30.3 bits (65), Expect = 0.40
Identities = 19/59 (32%), Positives = 19/59 (32%)
Frame = -3
Query: 620 PXXPPPTXXXPXPPPXXPXXXPXFXXXXXXXPKXXXPPPPXXXXXFXPXQKXXPPPXPP 444
P PPP P P P P P PPPP P PPP PP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPP----PPPPPGVAGAGPPP---PPPPPP 783
Score = 29.1 bits (62), Expect = 0.93
Identities = 11/26 (42%), Positives = 13/26 (50%)
Frame = -2
Query: 306 PSPPTXXXFFTPPXPPLXXXPPPPPP 229
P+P PP P + PPPPPP
Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPP 767
Score = 27.5 bits (58), Expect = 2.8
Identities = 17/49 (34%), Positives = 17/49 (34%), Gaps = 4/49 (8%)
Frame = -1
Query: 526 PKXXXPPPPXXPXPXPPXK----XXXPPPXPXKXKKKTPPLXXPXFPPP 392
P P P P P PP PPP P PP P PPP
Sbjct: 737 PAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP--PPP 783
Score = 26.6 bits (56), Expect = 5.0
Identities = 13/31 (41%), Positives = 13/31 (41%), Gaps = 5/31 (16%)
Frame = -2
Query: 306 PSPPTXXXFFTPPXPP-----LXXXPPPPPP 229
P PP PP PP PPPPPP
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 25.0 bits (52), Expect(2) = 2.1
Identities = 11/34 (32%), Positives = 11/34 (32%)
Frame = -3
Query: 509 PPPXXXXXFXPXQKXXPPPXPPXKXKKNXPPXXP 408
PPP P P P PP PP P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPP 765
Score = 21.0 bits (42), Expect(2) = 2.1
Identities = 7/13 (53%), Positives = 7/13 (53%)
Frame = -3
Query: 308 PPPPPHXXXFSPP 270
PPPPP PP
Sbjct: 764 PPPPPGVAGAGPP 776
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 31.1 bits (67), Expect = 0.23
Identities = 20/67 (29%), Positives = 20/67 (29%), Gaps = 3/67 (4%)
Frame = -3
Query: 608 PPTXXXPXP--PPXXPXXXPXFXXXXXXXPKXXXPP-PPXXXXXFXPXQKXXPPPXPPXK 438
PPT P P PP P P P PP P P P PP
Sbjct: 139 PPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMP 198
Query: 437 XKKNXPP 417
K PP
Sbjct: 199 PKVPPPP 205
Score = 29.9 bits (64), Expect = 0.53
Identities = 17/73 (23%), Positives = 18/73 (24%)
Frame = -1
Query: 610 PPXXXXXPXPPPXXPXXXXXFXXXXKXXPKXXXPPPPXXPXPXPPXKXXXPPPXPXKXKK 431
P P P P P P P P P P PP
Sbjct: 133 PQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPS 192
Query: 430 KTPPLXXPXFPPP 392
PP+ PPP
Sbjct: 193 AVPPMPPKVPPPP 205
Score = 26.6 bits (56), Expect = 5.0
Identities = 12/27 (44%), Positives = 13/27 (48%), Gaps = 2/27 (7%)
Frame = -2
Query: 306 PSPPTXXXFFTPP--XPPLXXXPPPPP 232
P+PPT PP PP PPP P
Sbjct: 128 PAPPTPQSELRPPTSAPPRPSIPPPSP 154
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.1 bits (62), Expect = 0.93
Identities = 13/43 (30%), Positives = 14/43 (32%)
Frame = -1
Query: 526 PKXXXPPPPXXPXPXPPXKXXXPPPXPXKXKKKTPPLXXPXFP 398
P+ PP P P PP PPP P P P
Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 28.7 bits (61), Expect = 1.2
Identities = 13/40 (32%), Positives = 13/40 (32%)
Frame = -1
Query: 511 PPPPXXPXPXPPXKXXXPPPXPXKXKKKTPPLXXPXFPPP 392
PPPP P PP P P P P P P
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
Score = 27.1 bits (57), Expect = 3.8
Identities = 17/65 (26%), Positives = 18/65 (27%)
Frame = -3
Query: 578 PXXPXXXPXFXXXXXXXPKXXXPPPPXXXXXFXPXQKXXPPPXPPXKXKKNXPPXXPXFS 399
P P P P+ P PP P P P P PP P S
Sbjct: 1683 PAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSA---PPPPLPASS 1739
Query: 398 PPXXP 384
P P
Sbjct: 1740 APSVP 1744
Score = 27.1 bits (57), Expect = 3.8
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = -2
Query: 276 TPPXPPLXXXPPPPPP 229
TPP PP+ PPP P
Sbjct: 1706 TPPPPPMSVPPPPSAP 1721
Score = 26.6 bits (56), Expect = 5.0
Identities = 13/30 (43%), Positives = 15/30 (50%), Gaps = 4/30 (13%)
Frame = -2
Query: 306 PSPPTXXXFFTPPX--PPLXXXPP--PPPP 229
P+PP PP PP+ PP PPPP
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 25.8 bits (54), Expect = 8.7
Identities = 11/35 (31%), Positives = 11/35 (31%)
Frame = -1
Query: 526 PKXXXPPPPXXPXPXPPXKXXXPPPXPXKXKKKTP 422
P PPPP P PPP P P
Sbjct: 1710 PPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.5 bits (58), Expect = 2.8
Identities = 20/81 (24%), Positives = 21/81 (25%), Gaps = 2/81 (2%)
Frame = -3
Query: 620 PXXPPPTXXXPXPPPXX--PXXXPXFXXXXXXXPKXXXPPPPXXXXXFXPXQKXXPPPXP 447
P P P P P P P P PP P P P P
Sbjct: 1141 PVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAP-P 1199
Query: 446 PXKXKKNXPPXXPXFSPPXXP 384
K PP P + P P
Sbjct: 1200 VPKPSVGVPPVPPPSTAPPVP 1220
Score = 27.1 bits (57), Expect = 3.8
Identities = 16/66 (24%), Positives = 17/66 (25%)
Frame = -1
Query: 589 PXPPPXXPXXXXXFXXXXKXXPKXXXPPPPXXPXPXPPXKXXXPPPXPXKXKKKTPPLXX 410
P P P P PP P PP P K PP+
Sbjct: 1153 PAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPP 1212
Query: 409 PXFPPP 392
P PP
Sbjct: 1213 PSTAPP 1218
Score = 26.6 bits (56), Expect = 5.0
Identities = 19/80 (23%), Positives = 21/80 (26%), Gaps = 1/80 (1%)
Frame = -3
Query: 620 PXXPPPTXXXPXPPPXXPXXXPXFXXXXXXXPKXXXPPPPXXXXXFXPXQKXXPPPXPPX 441
P P P+ P P P P PK PP P PP
Sbjct: 1074 PSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPV 1133
Query: 440 KXKKNXPP-XXPXFSPPXXP 384
PP P + P P
Sbjct: 1134 PVPSGAPPVPKPSVAAPPVP 1153
Score = 26.6 bits (56), Expect = 5.0
Identities = 13/45 (28%), Positives = 14/45 (31%)
Frame = -1
Query: 526 PKXXXPPPPXXPXPXPPXKXXXPPPXPXKXKKKTPPLXXPXFPPP 392
P PP P PP P K PP+ P PP
Sbjct: 1097 PSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPP 1141
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 27.1 bits (57), Expect = 3.8
Identities = 19/65 (29%), Positives = 21/65 (32%)
Frame = +3
Query: 396 GGKXGXXRGGVFFXFXXGXGGGXXFLXGGKGXGXXGGGGXXXFGXXXXXXXKXWXXXGXX 575
G + G G F G GG GG G GGG FG + G
Sbjct: 129 GARNGPAGRGGRGGFRGGRGGSRGGF-GGNSRGGFGGGSRGGFGGGSRGGSRGGFRGGSR 187
Query: 576 GGGXG 590
GG G
Sbjct: 188 GGFRG 192
Score = 25.8 bits (54), Expect = 8.7
Identities = 17/46 (36%), Positives = 17/46 (36%)
Frame = +3
Query: 372 GXCGRXXGGGKXGXXRGGVFFXFXXGXGGGXXFLXGGKGXGXXGGG 509
G GR G G RGG G GGG GG G GG
Sbjct: 136 GRGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRGGFGGGSRGGSRGG 181
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.6 bits (56), Expect = 5.0
Identities = 20/78 (25%), Positives = 22/78 (28%), Gaps = 1/78 (1%)
Frame = -2
Query: 459 PPPSPXKXKKKXPPXSX-LXFPPPXTXHKXPXTXXXXXXXXXXXXPXSFXFXPSPPTXXX 283
PPPS P S + PP P S SP
Sbjct: 167 PPPSFQPPSAAAPATSLPSDYNPPPPPPPPPAVEDQAADANEPDDYYSSGRAVSPEIPPT 226
Query: 282 FFTPPXPPLXXXPPPPPP 229
+ PL PPPPPP
Sbjct: 227 YTPKQADPLPAPPPPPPP 244
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 26.6 bits (56), Expect = 5.0
Identities = 13/39 (33%), Positives = 14/39 (35%), Gaps = 1/39 (2%)
Frame = -1
Query: 508 PPPXXPXPXPPXKXXXP-PPXPXKXKKKTPPLXXPXFPP 395
P P P PP P P P + PP P PP
Sbjct: 42 PAPVSKKPLPPPTRRLPRKPLPFRSTSLQPPSSQPPAPP 80
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 26.2 bits (55), Expect = 6.6
Identities = 17/44 (38%), Positives = 18/44 (40%)
Frame = +3
Query: 381 GRXXGGGKXGXXRGGVFFXFXXGXGGGXXFLXGGKGXGXXGGGG 512
GR GG G RGG G GG GG+G G GG
Sbjct: 24 GRGGFGGGRGGARGGGRGGARGGRGGRGG-ARGGRGGSSGGRGG 66
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.159 0.534
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,430,000
Number of Sequences: 5004
Number of extensions: 24323
Number of successful extensions: 246
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 37
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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