Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP16_F_P23
(926 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 104 2e-23
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 70 5e-13
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 69 7e-13
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 57 3e-09
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 36 0.011
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 34 0.025
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 33 0.057
SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 33 0.075
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 32 0.100
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 29 0.70
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 29 1.2
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 28 1.6
SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha... 28 2.1
SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 2.8
SPCC1672.09 |||triglyceride lipase-cholesterol esterase |Schizos... 26 6.6
SPCC16C4.10 |||6-phosphogluconolactonase |Schizosaccharomyces po... 26 8.7
SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate syn... 26 8.7
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 104 bits (249), Expect = 2e-23
Identities = 46/144 (31%), Positives = 92/144 (63%)
Frame = +2
Query: 128 EQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINF 307
++++A R+AF +FD + G I ++ ++ ++GQ +ELQ +I+E D + +G I+F
Sbjct: 8 DEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDF 67
Query: 308 DGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLD 487
F + + +++ D E +E++EAF+++D++GNGYIT L +L +L ++LS ++
Sbjct: 68 TEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124
Query: 488 GIXAEIDTDGSGTVDFDEFMEMMT 559
+ E DTDG G ++++EF +++
Sbjct: 125 DMIREADTDGDGVINYEEFSRVIS 148
Score = 26.6 bits (56), Expect = 5.0
Identities = 21/106 (19%), Positives = 43/106 (40%), Gaps = 1/106 (0%)
Frame = +2
Query: 140 AMLRKAFQMFDTTKSGYIDVLKISTIL-NTMGQLFDDSELQALIDENDPENSGKINFDGF 316
A L+ D +G ID + T++ M ++ E++ D + +G I +
Sbjct: 48 AELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEEL 107
Query: 317 CNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAA 454
+H L Q+E+ + R D +G+G I ++++
Sbjct: 108 ----THVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISS 149
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 69.7 bits (163), Expect = 5e-13
Identities = 39/145 (26%), Positives = 80/145 (55%)
Frame = +2
Query: 116 IPDDEQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSG 295
+P +++ +++AF ++D K G I + ++L ++G D+EL L +E
Sbjct: 1 MPASKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GD 56
Query: 296 KINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSN 475
I+ F + S+ L E ++E +E +AFR++D++ +GYI T+ + + L +KLS+
Sbjct: 57 AIDEKKFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSD 113
Query: 476 ADLDGIXAEIDTDGSGTVDFDEFME 550
++ + E D SG+ D+ +F++
Sbjct: 114 NEVQLMVQEADPTNSGSFDYYDFVQ 138
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 69.3 bits (162), Expect = 7e-13
Identities = 34/143 (23%), Positives = 73/143 (51%)
Frame = +2
Query: 128 EQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINF 307
E++ + +AF++FD+ K ID ++ + +G + SE+ ++ + D G +
Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92
Query: 308 DGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLD 487
+ F + + + E D +E+K AF L+D + G I+ L+ + L++ + + +L+
Sbjct: 93 EDFVRVMTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Query: 488 GIXAEIDTDGSGTVDFDEFMEMM 556
+ E D D G ++ EF+ +M
Sbjct: 150 AMIEEFDLDQDGEINEQEFIAIM 172
Score = 41.1 bits (92), Expect = 2e-04
Identities = 20/74 (27%), Positives = 38/74 (51%)
Frame = +2
Query: 338 LEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIXAEIDTDG 517
L E E +Q++ EAF+L+D + + I L+ + AL +++ I + D G
Sbjct: 27 LRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTG 86
Query: 518 SGTVDFDEFMEMMT 559
G + ++F+ +MT
Sbjct: 87 KGYLQMEDFVRVMT 100
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 57.2 bits (132), Expect = 3e-09
Identities = 42/150 (28%), Positives = 76/150 (50%), Gaps = 6/150 (4%)
Frame = +2
Query: 131 QKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFD 310
+++ +RK F D +SG ID + +I + S L +++DE + G ++F
Sbjct: 21 EEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDE---DGGGDVDFQ 76
Query: 311 GFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAAL------DDKLS 472
F N S F + E ++LK AF++YD + +GYI+ L +L + +D+L
Sbjct: 77 EFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQ 133
Query: 473 NADLDGIXAEIDTDGSGTVDFDEFMEMMTG 562
+D E+D D G + F+EF ++++G
Sbjct: 134 QI-VDKTIMEVDKDRDGKISFEEFKDIVSG 162
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 35.5 bits (78), Expect = 0.011
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = +2
Query: 368 QELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIXAEID 508
QELKEAF L D++G+G I +K +L +L+ S ++ + I+
Sbjct: 48 QELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 34.3 bits (75), Expect = 0.025
Identities = 17/60 (28%), Positives = 35/60 (58%)
Frame = +2
Query: 377 KEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIXAEIDTDGSGTVDFDEFMEMM 556
K+AF L+DR G G I +++ ++L A + A++ EI++ VD ++F++++
Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI----TEIESTLPAEVDMEQFLQVL 64
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 33.1 bits (72), Expect = 0.057
Identities = 22/65 (33%), Positives = 32/65 (49%), Gaps = 1/65 (1%)
Frame = +2
Query: 362 MQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIXAEIDTDG-SGTVDFD 538
M +E +EAF L+D GYI L+ A L + L+ L +D G +G V +
Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQ---LMLDLAGTNGKVSRE 66
Query: 539 EFMEM 553
EF E+
Sbjct: 67 EFAEL 71
Score = 32.7 bits (71), Expect = 0.075
Identities = 17/61 (27%), Positives = 30/61 (49%)
Frame = +2
Query: 152 KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIAS 331
+AF +FD T GYID + +G+ +LQ ++D +GK++ + F +
Sbjct: 16 EAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDL--AGTNGKVSREEFAELWI 73
Query: 332 H 334
H
Sbjct: 74 H 74
>SPBC11G11.02c |end3||actin cortical patch component End3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 375
Score = 32.7 bits (71), Expect = 0.075
Identities = 22/55 (40%), Positives = 29/55 (52%)
Frame = +2
Query: 380 EAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIXAEIDTDGSGTVDFDEF 544
E FR + E NGY++ S +L + KLS+ L+ I D D G DFDEF
Sbjct: 11 EIFRGLNPE-NGYLSGSKAAGVLRS--SKLSSDKLEKIWDLADIDDDGMFDFDEF 62
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 32.3 bits (70), Expect = 0.100
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +2
Query: 344 EEDAEAMQQELKEAFRLYDREGNGYITTSTLKE 442
++D M Q LKE Y+ E N IT+ TLKE
Sbjct: 522 KDDLTEMNQRLKEQIESYENEVNSEITSRTLKE 554
>SPAC23C11.01 |||ER membrane protein, ICE2
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 29.5 bits (63), Expect = 0.70
Identities = 17/46 (36%), Positives = 27/46 (58%)
Frame = +2
Query: 200 LKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHF 337
+ +ST+ +T + DDS L+ +ID+ PE GK NI++HF
Sbjct: 12 ISVSTVEDTSFEAMDDSILE-IIDDEAPEIQGKPK----ANISTHF 52
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 28.7 bits (61), Expect = 1.2
Identities = 29/116 (25%), Positives = 44/116 (37%)
Frame = +2
Query: 209 STILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAF 388
ST + + ++ D+ Q+ +D +S D NI + E+ + Q F
Sbjct: 256 STKSSKLDEISADNNAQSAVDNK--YSSPPQTIDPQANIKALITPEDRSNFYQ-----LF 308
Query: 389 RLYDREGNGYITTSTLKEILAALDDKLSNADLDGIXAEIDTDGSGTVDFDEFMEMM 556
D E GYI A L + +L I +DT G +D DEF M
Sbjct: 309 SKIDNENKGYIVGGEAVPFFMA--SHLDSEELARIWDTVDTQDRGYIDKDEFAVAM 362
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 28.3 bits (60), Expect = 1.6
Identities = 27/112 (24%), Positives = 49/112 (43%), Gaps = 1/112 (0%)
Frame = +2
Query: 125 DEQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSEL-QALIDENDPENSGKI 301
D++++ K F F SG+++ + I D S + + + D + +G I
Sbjct: 23 DKKELQQWYKGF--FKDCPSGHLNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYI 80
Query: 302 NFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAAL 457
+F F S E + +L AF+LYD + NG I+ + I+ A+
Sbjct: 81 DFKEFICALSVTSRGE----LNDKLIWAFQLYDLDNNGLISYDEMLRIVDAI 128
>SPBC14F5.12c |cbh2||centromere binding protein
Cbh2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 27.9 bits (59), Expect = 2.1
Identities = 19/81 (23%), Positives = 40/81 (49%), Gaps = 3/81 (3%)
Frame = +2
Query: 194 DVLKISTILNTM-GQLFDDSELQALIDENDP--ENSGKINFDGFCNIASHFLEEEDAEAM 364
+ ++I+ L + G+ D ++ + + +P ENS N D +A+ FL+E + E
Sbjct: 402 ETIRIAVNLQELIGKYLGDKDIFQIENFINPIEENSADTNDDIVNQVAAQFLDEREFETD 461
Query: 365 QQELKEAFRLYDREGNGYITT 427
++E + + L ++ I T
Sbjct: 462 EEEEESQYLLSTKDAINAINT 482
>SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 418
Score = 27.5 bits (58), Expect = 2.8
Identities = 15/68 (22%), Positives = 29/68 (42%)
Frame = +2
Query: 266 IDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEI 445
+ E + F F + ++ +D A+ Q + +F +R G G I T ++
Sbjct: 343 VSEESQVEPNTLIFQNFSSPSTSLSAPKDTPAVIQSINTSFLNNERAGRGNIKTVDQNQL 402
Query: 446 LAALDDKL 469
+ + DKL
Sbjct: 403 INRIVDKL 410
>SPCC1672.09 |||triglyceride lipase-cholesterol esterase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 467
Score = 26.2 bits (55), Expect = 6.6
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = +3
Query: 93 PILDVQHGFLMMNKRWPCCVKHSRCL 170
P++ HG LM ++ W C V CL
Sbjct: 127 PVVYCHHGLLMNSEVWVCNVDPRNCL 152
>SPCC16C4.10 |||6-phosphogluconolactonase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 257
Score = 25.8 bits (54), Expect = 8.7
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +2
Query: 62 SHTDSVLVTAANP*RATWIPDDEQKMAMLRKAFQMF 169
S S L+T N R +W DDE + R + ++F
Sbjct: 220 SKLPSALITRNNLTRTSWFVDDEASANLERSSLKVF 255
>SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate
synthase Lsp1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.7
Identities = 20/100 (20%), Positives = 41/100 (41%), Gaps = 5/100 (5%)
Frame = +2
Query: 266 IDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQ---ELKEAFRLYDREGNGYITTSTL 436
I +N P + IN + ++ EE E KE LY+++ G++ +
Sbjct: 181 IMKNSPMPAEPINKENINQLSRSLFYEEGKEFWDDYDIAAKEILSLYEQDPEGFVNPYSK 240
Query: 437 KEILAALDDKLSNADLDGIXAEI--DTDGSGTVDFDEFME 550
+ A + + + G+ A+ D D + +EF++
Sbjct: 241 RGAAALANSENNKGSEAGVSAKTNPDMDSDSSAIVNEFLK 280
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,625,088
Number of Sequences: 5004
Number of extensions: 47434
Number of successful extensions: 155
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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