Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP16_F_P15
(895 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 64 3e-11
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 63 6e-11
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 49 8e-07
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 29 0.67
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 28 1.6
SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr... 27 3.6
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 3.6
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 63.7 bits (148), Expect = 3e-11
Identities = 41/136 (30%), Positives = 68/136 (50%)
Frame = +1
Query: 127 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 306
AFS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 307 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTXXXXXXXX 486
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDEL---LKGVPV 124
Query: 487 XXXMIPYAAFLKKVMA 534
M+ Y F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 62.9 bits (146), Expect = 6e-11
Identities = 41/128 (32%), Positives = 65/128 (50%), Gaps = 3/128 (2%)
Frame = +1
Query: 85 MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 258
M+ + D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60
Query: 259 XXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 435
FL + ++ KD D E+ E K++DK+ NG + ELTH L +LGE+L
Sbjct: 61 GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118
Query: 436 DDSEVAEV 459
EVA++
Sbjct: 119 SQEEVADM 126
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 49.2 bits (112), Expect = 8e-07
Identities = 30/118 (25%), Positives = 56/118 (47%)
Frame = +1
Query: 97 SKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 276
SK + AF +YD + G I ++G +LR+L N T A +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 277 XXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEV 450
F+ + K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV
Sbjct: 64 MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEV 116
Score = 27.1 bits (57), Expect = 3.6
Identities = 12/43 (27%), Positives = 27/43 (62%)
Frame = +1
Query: 334 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVT 462
++ E LYD +++GL+ + + L +LG + D+E+A+++
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLS 51
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 29.5 bits (63), Expect = 0.67
Identities = 27/134 (20%), Positives = 52/134 (38%), Gaps = 1/134 (0%)
Frame = +1
Query: 52 RPGGNRQTPHKMSDLSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIX 231
R GG ++++ + D+ A F ++D + ID L +RAL N + +
Sbjct: 20 RLGGYAPLRVEITEEQRQDINEA---FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVL 76
Query: 232 XXXXXXXXXXXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTH 408
F+ + ++ ++D E+ +L+D +E G + L
Sbjct: 77 KILRDFDKTGKGYLQMEDFVRVMTEKIVERDP--LEEIKRAFELFDDDETGKISLRNLRR 134
Query: 409 TLLALGEKLDDSEV 450
L E +DD E+
Sbjct: 135 VAKELNENIDDQEL 148
Score = 26.6 bits (56), Expect = 4.8
Identities = 14/55 (25%), Positives = 28/55 (50%)
Frame = +1
Query: 295 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEV 459
Y+ + + + +D E KL+D +++ + EL + ALG + SEV ++
Sbjct: 24 YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKI 78
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 28.3 bits (60), Expect = 1.6
Identities = 31/157 (19%), Positives = 63/157 (40%)
Frame = +2
Query: 26 GILKIXASEDREGTDKHHTK*ATSARTTLKGRLSPSQSTTLKAKARSMPSTLAIS*ERST 205
G+L+ S + + ++ + ++ SPS S++ + + S S+ + S S+
Sbjct: 121 GVLQTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSS 180
Query: 206 QTPHWQPSRNSVVQRRRARSCSHSKSSFPSTAKQRKTKTRERMKISWNV*SCMTRTKTVS 385
+ S +S + S S S SS P T+ + + S + S + +
Sbjct: 181 SSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSSSSSSSRPSSSSSF 240
Query: 386 CLALSSHTRFLH*VRSLTTVKSLKSQXTAWTLKTMTA 496
+SS T + ++T S S T+ + + TA
Sbjct: 241 ITTMSSSTF----ISTVTVTPSSSSSSTSSEVPSSTA 273
>SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 178
Score = 27.1 bits (57), Expect = 3.6
Identities = 22/97 (22%), Positives = 43/97 (44%), Gaps = 2/97 (2%)
Frame = +2
Query: 59 EGTD-KHHTK*ATSARTTLKGRLSPSQSTTLKAKAR-SMPSTLAIS*ERSTQTPHWQPSR 232
E TD ++HT T A+ KG P++ KAK + ++ +S Q P++
Sbjct: 38 ERTDYRNHTSCMTEAQRYQKGLYRPTKKELKKAKMNGNAVNSKELSPNTDNQNTPAGPTK 97
Query: 233 NSVVQRRRARSCSHSKSSFPSTAKQRKTKTRERMKIS 343
+S+ + + + SK S+ ++ + +IS
Sbjct: 98 HSLDENEKDKENKKSKKETVSSPAEQLLALTQNQEIS 134
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 3.6
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +1
Query: 85 MSDLSKNDVER-ASFAFSIYDFEGKGKID 168
+++L DV R SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,651,786
Number of Sequences: 5004
Number of extensions: 41964
Number of successful extensions: 110
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 106
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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