Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP16_F_P12
(870 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 69 7e-13
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 57 3e-09
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 52 1e-07
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 45 2e-05
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 44 2e-05
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 44 2e-05
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 32 0.12
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 31 0.21
SPBC14C8.17c |||SAGA complex subunit Spt8 |Schizosaccharomyces p... 26 6.1
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 26 8.0
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 69.3 bits (162), Expect = 7e-13
Identities = 32/71 (45%), Positives = 52/71 (73%), Gaps = 1/71 (1%)
Frame = +1
Query: 172 TEENVLVLSXANFETVI-STTEYILVEFYAPWCGHCKSLAPEYAXAATKLAEEESPIKLA 348
++E+++VL NF+ ++ T+ +LVEFYAPWCGHCK+LAP Y A + + ++S + +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYS-DDSNVVVA 411
Query: 349 KVDATQEQDLA 381
K+DAT E D++
Sbjct: 412 KIDAT-ENDIS 421
Score = 68.1 bits (159), Expect = 2e-12
Identities = 31/70 (44%), Positives = 44/70 (62%)
Frame = +1
Query: 217 VISTTEYILVEFYAPWCGHCKSLAPEYAXAATKLAEEESPIKLAKVDATQEQDLARELRC 396
+I+ + ++V+FYAPWCGHCK+LAPEY AA +L E+ I L +VD T+E DL E
Sbjct: 35 LITADKVLMVKFYAPWCGHCKALAPEYESAADEL--EKDGISLVEVDCTEEGDLCSEYSI 92
Query: 397 ARIPDSQILQ 426
P + +
Sbjct: 93 RGYPTLNVFK 102
Score = 41.1 bits (92), Expect = 2e-04
Identities = 17/40 (42%), Positives = 26/40 (65%), Gaps = 1/40 (2%)
Frame = +2
Query: 389 YGVRGYPTLKFFRNGSPI-DYSGGRQAXDIIXWLKKKTAP 505
Y +RGYPTL F+NG I YSG R+ ++ +++K+ P
Sbjct: 90 YSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLP 129
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/39 (30%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
Frame = +2
Query: 386 SYGVRGYPTLKFFRNG---SPIDYSGGRQAXDIIXWLKK 493
S + G+PT+ FF+ +P+ Y G R D+ ++ K
Sbjct: 421 SVSISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 57.2 bits (132), Expect = 3e-09
Identities = 32/98 (32%), Positives = 48/98 (48%)
Frame = +1
Query: 115 VLIFTAIALLGLALGDEVPTEENVLVLSXANFETVISTTEYILVEFYAPWCGHCKSLAPE 294
+L F AL L V +++ L T+ ++ + L+EFYA WCGHCKSLAP
Sbjct: 5 LLSFVIFALFALVFASGVVELQSLNELE----NTIRASKKGALIEFYATWCGHCKSLAPV 60
Query: 295 YAXAATKLAEEESPIKLAKVDATQEQDLARELRCARIP 408
Y L E+ + + + K+DA D+A + P
Sbjct: 61 YEELGA-LFEDHNDVLIGKIDADTHSDVADKYHITGFP 97
Score = 56.8 bits (131), Expect = 4e-09
Identities = 29/77 (37%), Positives = 43/77 (55%), Gaps = 1/77 (1%)
Frame = +1
Query: 181 NVLVLSXANFETVISTTEY-ILVEFYAPWCGHCKSLAPEYAXAATKLAEEESPIKLAKVD 357
NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Y K+ + E +++ K++
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLG-KVFKNEPNVEIVKIN 199
Query: 358 ATQEQDLARELRCARIP 408
A D+ R A P
Sbjct: 200 ADVFADIGRLHEVASFP 216
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 52.0 bits (119), Expect = 1e-07
Identities = 28/102 (27%), Positives = 43/102 (42%)
Frame = +1
Query: 118 LIFTAIALLGLALGDEVPTEENVLVLSXANFETVISTTEYILVEFYAPWCGHCKSLAPEY 297
L +L+ G N + L+ NF + LV FYAPWCG+CK L P Y
Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70
Query: 298 AXAATKLAEEESPIKLAKVDATQEQDLARELRCARIPDSQIL 423
A+ L P+ DA Q + + + + P +++
Sbjct: 71 QKLASNL-HSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLV 111
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 44.8 bits (101), Expect = 2e-05
Identities = 20/73 (27%), Positives = 39/73 (53%)
Frame = +1
Query: 190 VLSXANFETVISTTEYILVEFYAPWCGHCKSLAPEYAXAATKLAEEESPIKLAKVDATQE 369
V + F++++ + ++V+F+A WCG CK++AP++ + + S KVD Q
Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF----EQFSNTYSDATFIKVDVDQL 60
Query: 370 QDLARELRCARIP 408
++A E +P
Sbjct: 61 SEIAAEAGVHAMP 73
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 44.4 bits (100), Expect = 2e-05
Identities = 21/73 (28%), Positives = 41/73 (56%)
Frame = +1
Query: 190 VLSXANFETVISTTEYILVEFYAPWCGHCKSLAPEYAXAATKLAEEESPIKLAKVDATQE 369
+ S ++ + I + Y+ V+ YA WCG CK+++P ++ A+K A + AKV+ ++
Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYASPK--FVFAKVNVDEQ 63
Query: 370 QDLARELRCARIP 408
+ +A L +P
Sbjct: 64 RQIASGLGVKAMP 76
Score = 26.2 bits (55), Expect = 6.1
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +2
Query: 392 GVRGYPTLKFFRNGSPIDYSGG 457
GV+ PT FF NG ID G
Sbjct: 71 GVKAMPTFVFFENGKQIDMLTG 92
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 44.4 bits (100), Expect = 2e-05
Identities = 25/82 (30%), Positives = 41/82 (50%)
Frame = +1
Query: 190 VLSXANFETVISTTEYILVEFYAPWCGHCKSLAPEYAXAATKLAEEESPIKLAKVDATQE 369
V S ++ T IS + +V+FYA WCG CK L P KL+E+ V+A +
Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQNQKASFIAVNADKF 77
Query: 370 QDLARELRCARIPDSQILQEWQ 435
D+A++ +P + ++ Q
Sbjct: 78 SDIAQKNGVYALPTMVLFRKGQ 99
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 31.9 bits (69), Expect = 0.12
Identities = 19/69 (27%), Positives = 32/69 (46%), Gaps = 2/69 (2%)
Frame = +1
Query: 193 LSXANFETVISTTEYILVEFYAPWCGHCKSLAPEYAXAATKLAE--EESPIKLAKVDATQ 366
L+ + E+ +S + +++Y P CG CK L P + K E E S +VD ++
Sbjct: 31 LTDNDLESEVSKGTWF-IKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSK 89
Query: 367 EQDLARELR 393
E +R
Sbjct: 90 ELSSCANIR 98
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 31.1 bits (67), Expect = 0.21
Identities = 15/59 (25%), Positives = 27/59 (45%)
Frame = +1
Query: 232 EYILVEFYAPWCGHCKSLAPEYAXAATKLAEEESPIKLAKVDATQEQDLARELRCARIP 408
+ IL+ FYAPW CK + + + A++ K++A + D+A +P
Sbjct: 21 QIILLNFYAPWAAPCKQMNQVF----DQFAKDTKNAVFLKIEAEKFSDIAESFDVNAVP 75
>SPBC14C8.17c |||SAGA complex subunit Spt8 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 526
Score = 26.2 bits (55), Expect = 6.1
Identities = 16/39 (41%), Positives = 18/39 (46%)
Frame = +2
Query: 398 RGYPTLKFFRNGSPIDYSGGRQAXDIIXWLKKKTAPPAC 514
RG L NG I YS Q + LKK TAP +C
Sbjct: 199 RGLWVLSGVNNGDIILYSTRHQEGYPVTSLKKHTAPVSC 237
>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
Srb9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1223
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/41 (31%), Positives = 18/41 (43%)
Frame = +2
Query: 179 KMCSF*VKLTLKL*FQPRSTF*LNSMLHGAATANLWHRNTP 301
K +F L QP T N +L+ + NLW R+ P
Sbjct: 620 KSANFDFSFLKSLDLQPTITLGKNDLLNAILSQNLWFRSLP 660
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,440,837
Number of Sequences: 5004
Number of extensions: 36298
Number of successful extensions: 97
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 89
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 96
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 434475230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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