BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP16_F_P09
(885 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 33 0.054
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.29
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 29 0.67
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 24 0.72
SPAC56E4.07 |||N-acetyltransferase |Schizosaccharomyces pombe|ch... 29 0.88
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 23 1.5
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.0
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 28 2.0
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 3.6
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 27 3.6
SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S... 27 4.7
SPAC20H4.05c |||adducin|Schizosaccharomyces pombe|chr 1|||Manual 27 4.7
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 26 8.2
SPBP8B7.16c |dbp2||ATP-dependent RNA helicase Dbp2|Schizosacchar... 26 8.2
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 33.1 bits (72), Expect = 0.054
Identities = 13/22 (59%), Positives = 14/22 (63%), Gaps = 3/22 (13%)
Frame = +3
Query: 816 PPPPPPXPX---PXRPPPPXXP 872
PPPPPP P P +PPPP P
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 30.7 bits (66), Expect = 0.29
Identities = 11/19 (57%), Positives = 11/19 (57%)
Frame = +3
Query: 816 PPPPPPXPXPXRPPPPXXP 872
PPPPPP PPPP P
Sbjct: 763 PPPPPPGVAGAGPPPPPPP 781
Score = 28.3 bits (60), Expect = 1.5
Identities = 11/22 (50%), Positives = 11/22 (50%)
Frame = +3
Query: 819 PPPPPXPXPXRPPPPXXPRSXA 884
P PPP P PPPP P A
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVA 771
Score = 27.9 bits (59), Expect = 2.0
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +3
Query: 816 PPPPPPXPXPXRPPPPXXP 872
PPPPPP PPP P
Sbjct: 762 PPPPPPPGVAGAGPPPPPP 780
Score = 27.9 bits (59), Expect = 2.0
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +3
Query: 816 PPPPPPXPXPXRPPPPXXP 872
PPPPP PPPP P
Sbjct: 764 PPPPPGVAGAGPPPPPPPP 782
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 29.5 bits (63), Expect = 0.67
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +3
Query: 816 PPPPPPXPXPXRPPPPXXPRS 878
PPPPPP P PP P S
Sbjct: 1882 PPPPPPMALPKAGPPSAAPTS 1902
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 24.2 bits (50), Expect(2) = 0.72
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = +3
Query: 819 PPPPPXPXPXRPP 857
P PPP P P PP
Sbjct: 236 PAPPPPPPPTLPP 248
Score = 23.4 bits (48), Expect(2) = 0.72
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = +3
Query: 816 PPPPPPXP 839
PPPPPP P
Sbjct: 189 PPPPPPPP 196
>SPAC56E4.07 |||N-acetyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 235
Score = 29.1 bits (62), Expect = 0.88
Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 2/39 (5%)
Frame = +3
Query: 183 QYEDKFLYFAYGSNLLAKRIHINNPT--AIFYSTAKLQD 293
+Y FLY+ YG+ ++ KR H N A Y T L++
Sbjct: 105 KYYSNFLYWWYGAQIVQKRFHYNELQYGAALYKTGLLKN 143
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 23.4 bits (48), Expect(2) = 1.5
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = +3
Query: 816 PPPPPPXP 839
PPPPPP P
Sbjct: 907 PPPPPPLP 914
Score = 23.0 bits (47), Expect(2) = 5.4
Identities = 7/12 (58%), Positives = 8/12 (66%)
Frame = +3
Query: 816 PPPPPPXPXPXR 851
P PPPP P P +
Sbjct: 905 PTPPPPPPLPVK 916
Score = 23.0 bits (47), Expect(2) = 1.5
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +3
Query: 819 PPPPPXPXP 845
PPPPP P P
Sbjct: 945 PPPPPPPPP 953
Score = 21.4 bits (43), Expect(2) = 5.4
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = +3
Query: 828 PPXPXPXRPPPP 863
P P P PPPP
Sbjct: 942 PAFPPPPPPPPP 953
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 2.0
Identities = 13/26 (50%), Positives = 13/26 (50%), Gaps = 3/26 (11%)
Frame = +3
Query: 816 PPPPPPXPXPXRP---PPPXXPRSXA 884
PPPP P P P PPP P S A
Sbjct: 1715 PPPPSAPPMPAGPPSAPPPPLPASSA 1740
Score = 26.6 bits (56), Expect = 4.7
Identities = 12/23 (52%), Positives = 12/23 (52%), Gaps = 3/23 (13%)
Frame = +3
Query: 819 PPPPPX---PXPXRPPPPXXPRS 878
PPPPP P P PP P P S
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPS 1729
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 27.9 bits (59), Expect = 2.0
Identities = 11/18 (61%), Positives = 11/18 (61%), Gaps = 2/18 (11%)
Frame = +3
Query: 816 PPPPPPXPXP--XRPPPP 863
PPPPPP P P P PP
Sbjct: 5 PPPPPPAPAPAAAAPAPP 22
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.1 bits (57), Expect = 3.6
Identities = 11/18 (61%), Positives = 11/18 (61%), Gaps = 2/18 (11%)
Frame = +3
Query: 816 PPPPPP--XPXPXRPPPP 863
PPPPPP P R PPP
Sbjct: 361 PPPPPPRSAPSTGRQPPP 378
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 27.1 bits (57), Expect = 3.6
Identities = 11/19 (57%), Positives = 11/19 (57%)
Frame = -3
Query: 874 RGXXGGGGRXGXGXGGGGG 818
RG GGG R G G G GG
Sbjct: 159 RGGFGGGSRGGFGGGSRGG 177
>SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein
Sad1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 26.6 bits (56), Expect = 4.7
Identities = 16/53 (30%), Positives = 27/53 (50%)
Frame = -3
Query: 415 LFKSLXFISQRSPHTYSXGSSTIVAALPFQSSVYMLKSNR*SCNLAVL*NIAV 257
L K+ +++ S T+S SS + A P Q+ + +KSN + N L + V
Sbjct: 431 LGKARYSLTEDSIQTFSFESSNYIVAEPIQNVILKIKSNWGNPNYTCLYQVRV 483
>SPAC20H4.05c |||adducin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 221
Score = 26.6 bits (56), Expect = 4.7
Identities = 13/39 (33%), Positives = 15/39 (38%)
Frame = +3
Query: 273 STAKLQDYRLDFNMYTELWNGNAATIVEDPXEYVWGDLW 389
+TA D L+ A IV D Y WGD W
Sbjct: 156 NTAHESDLHDSLQEAINLYPDTCAVIVRDHGIYCWGDTW 194
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 25.8 bits (54), Expect = 8.2
Identities = 8/10 (80%), Positives = 8/10 (80%)
Frame = +3
Query: 816 PPPPPPXPXP 845
PPPPPP P P
Sbjct: 356 PPPPPPMPAP 365
>SPBP8B7.16c |dbp2||ATP-dependent RNA helicase
Dbp2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 25.8 bits (54), Expect = 8.2
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = -3
Query: 874 RGXXGGGGRXGXGXGGGGGG 815
RG GGG R GGG GG
Sbjct: 22 RGRYGGGYRNNYSRGGGRGG 41
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,808,491
Number of Sequences: 5004
Number of extensions: 52062
Number of successful extensions: 292
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 234
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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