Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP16_F_O09
         (877 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|...   136   3e-33
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase...   133   4e-32

>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
           Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score =  136 bits (330), Expect = 3e-33
 Identities = 65/96 (67%), Positives = 76/96 (79%)
 Frame = +1

Query: 319 LVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 498
           LV++G+ I V +ERDP  I W  +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAPS 
Sbjct: 67  LVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAPSK 126

Query: 499 DAPMFVVGVNLXAYDPSFKVISNASCTTKLSCPTCK 606
           DAPMFVVGVNL  ++PS KVISNASCTT    P  K
Sbjct: 127 DAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAK 162



 Score = 98.3 bits (234), Expect = 1e-21
 Identities = 46/63 (73%), Positives = 53/63 (84%), Gaps = 1/63 (1%)
 Frame = +3

Query: 135 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 311
           K+GINGFGRIGR+VLR +I  G  QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + 
Sbjct: 5   KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64

Query: 312 GFL 320
           G L
Sbjct: 65  GKL 67



 Score = 73.3 bits (172), Expect = 4e-14
 Identities = 44/84 (52%), Positives = 47/84 (55%)
 Frame = +2

Query: 584 NCLAPLAKVIHDNFEIVEGLMXXXXXXXXXXXXXDGPSGKLWRDGRGCSXKHHSWPLLGA 763
           NCLAPLAKVI+D F I EGLM             DGPS K WR GRG S         GA
Sbjct: 155 NCLAPLAKVINDTFGIEEGLMTTVHATTATQKTVDGPSKKDWRGGRGASANIIP-SSTGA 213

Query: 764 GQSCG*XYPLALNGXLTGMAFXVP 835
            ++ G   P ALNG LTGMAF VP
Sbjct: 214 AKAVGKVIP-ALNGKLTGMAFRVP 236


>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
           Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 336

 Score =  133 bits (321), Expect = 4e-32
 Identities = 63/96 (65%), Positives = 75/96 (78%)
 Frame = +1

Query: 319 LVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 498
           LV++GN I V +ERDP  I W  +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAPS 
Sbjct: 67  LVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAPSK 126

Query: 499 DAPMFVVGVNLXAYDPSFKVISNASCTTKLSCPTCK 606
           DAPM+VVGVN   ++PS KVISNASCTT    P  K
Sbjct: 127 DAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAK 162



 Score =  101 bits (241), Expect = 2e-22
 Identities = 47/63 (74%), Positives = 54/63 (85%), Gaps = 1/63 (1%)
 Frame = +3

Query: 135 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 311
           K+GINGFGRIGR+VLR A + K  QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D
Sbjct: 5   KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64

Query: 312 GFL 320
           G L
Sbjct: 65  GKL 67



 Score = 73.3 bits (172), Expect = 4e-14
 Identities = 44/84 (52%), Positives = 47/84 (55%)
 Frame = +2

Query: 584 NCLAPLAKVIHDNFEIVEGLMXXXXXXXXXXXXXDGPSGKLWRDGRGCSXKHHSWPLLGA 763
           NCLAPLAKVI+D F I EGLM             DGPS K WR GRG S         GA
Sbjct: 155 NCLAPLAKVINDTFGIEEGLMTTVHATTATQKTVDGPSKKDWRGGRGASANIIP-SSTGA 213

Query: 764 GQSCG*XYPLALNGXLTGMAFXVP 835
            ++ G   P ALNG LTGMAF VP
Sbjct: 214 AKAVGKVIP-ALNGKLTGMAFRVP 236


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,649,314
Number of Sequences: 5004
Number of extensions: 76675
Number of successful extensions: 220
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)