Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP16_F_N15
         (888 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar...   107   2e-24
SPAC1F5.10 |||ATP-dependent RNA helicase, eIF4A related|Schizosa...    73   4e-14
SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyc...    54   4e-08
SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccha...    46   1e-05
SPAC17G6.14c |uap56||ATP-dependent RNA helicase Uap56|Schizosacc...    39   0.001
SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac...    38   0.001
SPAC823.08c |||ATP-dependent RNA helicase Rrp3 |Schizosaccharomy...    38   0.003
SPCC1494.06c |||ATP-dependent RNA helicase Dbp9 |Schizosaccharom...    37   0.003
SPAC1F7.02c |||ATP-dependent RNA helicase Has1 |Schizosaccharomy...    36   0.006
SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S...    34   0.024
SPAC30D11.03 |drs1||ATP-dependent RNA helicase Drs1 |Schizosacch...    33   0.072
SPBP8B7.16c |dbp2||ATP-dependent RNA helicase Dbp2|Schizosacchar...    32   0.13 
SPCP1E11.03 |mug170||arrestin|Schizosaccharomyces pombe|chr 3|||...    30   0.51 
SPCC10H11.01 |prp11||ATP-dependent RNA helicase Prp11|Schizosacc...    29   0.88 
SPCC777.06c |||hydrolase |Schizosaccharomyces pombe|chr 3|||Manual     27   4.7  
SPBC530.12c |pdf1||palmitoyl protein thioesterase-dolichol pyrop...    27   4.7  

>SPAC1006.07 |||translation initiation factor
           eIF4A|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 392

 Score =  107 bits (258), Expect = 2e-24
 Identities = 75/190 (39%), Positives = 96/190 (50%), Gaps = 1/190 (0%)
 Frame = +3

Query: 213 DQVVETFDDMNLKEELLRGIYAYGFEKPSAIQQRAIMP-WHPRDAMLSLKAQSGTGKNCY 389
           D+V++TFDDMNLK ELLRGIYAYGFE+PSAIQQRAIMP    RD +   +AQSGTGK   
Sbjct: 15  DEVIDTFDDMNLKPELLRGIYAYGFERPSAIQQRAIMPILGERDVL--AQAQSGTGKTAT 72

Query: 390 FSLYRFYNKSIQAFVNVKL*SWAPTESWAHQFRKVVIAXG*SLEMLNAMPGIGGHQCP*R 569
           FS+     K   +    +    APT   A Q +KVV+A G  L  +     IGG      
Sbjct: 73  FSI-SVLQKIDTSLKQCQALILAPTRELAQQIQKVVVALG-DLMNVECHACIGGTLVRDD 130

Query: 570 YSPLXXXXXXXXXXXXXCI*YDNXSCRFMPPPIXLFWXXXXXXXXXXXXFKDQIHDVFKM 749
            + L                +D    R +P      +            FKDQI+D+F++
Sbjct: 131 MAALQAGVHVVVGTPGRV--HDMIQRRALPTDAVQMFVLDEADEMLSRGFKDQIYDIFQL 188

Query: 750 LSADVQVILL 779
           L    QV+LL
Sbjct: 189 LPPTAQVVLL 198



 Score = 26.2 bits (55), Expect = 6.2
 Identities = 17/39 (43%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
 Frame = +1

Query: 535 CLALVG-TNVREDIXHWXVVFMWWWGTPXPVYDMITRRA 648
           C A +G T VR+D+           GTP  V+DMI RRA
Sbjct: 118 CHACIGGTLVRDDMAALQAGVHVVVGTPGRVHDMIQRRA 156


>SPAC1F5.10 |||ATP-dependent RNA helicase, eIF4A
           related|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 394

 Score = 73.3 bits (172), Expect = 4e-14
 Identities = 49/107 (45%), Positives = 66/107 (61%), Gaps = 3/107 (2%)
 Frame = +3

Query: 198 LTPTGD-QVVETFDDMNLKEELLRGIYAYGFEKPSAIQQRAIMP-WHPRDAMLSLKAQSG 371
           LT + D   V +F++MNLKE+LLRGIYAYG+E PSA+Q RAI+     RD +   +AQSG
Sbjct: 11  LTTSEDVNAVSSFEEMNLKEDLLRGIYAYGYETPSAVQSRAIIQICKGRDVI--AQAQSG 68

Query: 372 TGKNCYFSLYRFYNKSIQAFV-NVKL*SWAPTESWAHQFRKVVIAXG 509
           TGK   FS+     +SI   V + +    +PT   A Q + VV+A G
Sbjct: 69  TGKTATFSIGIL--QSIDLSVRDTQALILSPTRELAVQIQNVVLALG 113


>SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 503

 Score = 53.6 bits (123), Expect = 4e-08
 Identities = 36/106 (33%), Positives = 59/106 (55%), Gaps = 2/106 (1%)
 Frame = +3

Query: 222 VETFDDMNLKEELLRGIYAYGFEKPSAIQQRA--IMPWHPRDAMLSLKAQSGTGKNCYFS 395
           V++F+++ LK ELL+GIY+  F+KPS IQ++A  ++  +P   M+  ++QSGTGK   F+
Sbjct: 113 VKSFEELELKPELLKGIYSMKFQKPSKIQEKALPLLLSNPPRNMIG-QSQSGTGKTAAFA 171

Query: 396 LYRFYNKSIQAFVNVKL*SWAPTESWAHQFRKVVIAXG*SLEMLNA 533
           L    ++   +    +    AP+   A Q   VV   G   E+  A
Sbjct: 172 L-TMLSRVDASVPKPQAICLAPSRELARQIMDVVTEMGKYTEVKTA 216


>SPBC776.09 |ste13||ATP-dependent RNA helicase
           Ste13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 485

 Score = 45.6 bits (103), Expect = 1e-05
 Identities = 25/55 (45%), Positives = 35/55 (63%), Gaps = 1/55 (1%)
 Frame = +3

Query: 231 FDDMNLKEELLRGIYAYGFEKPSAIQQRAI-MPWHPRDAMLSLKAQSGTGKNCYF 392
           F+D  LK ELL GI+  GFE+PS IQ+ +I +    RD +   +A++GTGK   F
Sbjct: 46  FEDYYLKRELLMGIFEAGFERPSPIQEESIPIALSGRDIL--ARAKNGTGKTAAF 98


>SPAC17G6.14c |uap56||ATP-dependent RNA helicase
           Uap56|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 434

 Score = 38.7 bits (86), Expect = 0.001
 Identities = 25/56 (44%), Positives = 29/56 (51%)
 Frame = +3

Query: 231 FDDMNLKEELLRGIYAYGFEKPSAIQQRAIMPWHPRDAMLSLKAQSGTGKNCYFSL 398
           F D  LK ELLR I   GFE PS +QQ  I P       +  +A+SG GK   F L
Sbjct: 53  FRDFLLKPELLRAITDSGFEHPSEVQQVCI-PQSILGTDVLCQAKSGMGKTAVFVL 107


>SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 848

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 31/98 (31%), Positives = 47/98 (47%), Gaps = 2/98 (2%)
 Frame = +3

Query: 210 GDQVVETFDDMNLKEELLRGIYAYGFEKPSAIQQRAI-MPWHPRDAMLSLKAQSGTGKNC 386
           G+     F  M L + LLR I+  GF+ P+ IQ++ I +    RD +    A++G+GK  
Sbjct: 64  GNGKASNFQSMGLNQTLLRAIFKKGFKAPTPIQRKTIPLLLEGRDVV--GMARTGSGKTA 121

Query: 387 YFSLYRF-YNKSIQAFVNVKL*SWAPTESWAHQFRKVV 497
            F +    + KS  A  N +    +P    A Q  KVV
Sbjct: 122 AFVIPMIEHLKSTLANSNTRALILSPNRELALQTVKVV 159


>SPAC823.08c |||ATP-dependent RNA helicase Rrp3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 465

 Score = 37.5 bits (83), Expect = 0.003
 Identities = 31/105 (29%), Positives = 50/105 (47%), Gaps = 3/105 (2%)
 Frame = +3

Query: 213 DQVVETFDDMNLKEELLRGIYAYGFEKPSAIQQRAIMPWHPRDAMLSLKAQSGTGKNCYF 392
           ++  +TF ++ + +EL       GF+ P+ IQQ AI     +  ++ L AQ+G+GK   F
Sbjct: 42  NEAPKTFKELGVIDELCEACEKLGFKTPTPIQQEAIPVVLNKRDVIGL-AQTGSGKTAAF 100

Query: 393 SL---YRFYNKSIQAFVNVKL*SWAPTESWAHQFRKVVIAXG*SL 518
           +L      +N     F  V     APT   A+Q  +   A G S+
Sbjct: 101 ALPVIQELWNNPSPFFAVV----LAPTRELAYQISEQFEAIGGSI 141


>SPCC1494.06c |||ATP-dependent RNA helicase Dbp9
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 595

 Score = 37.1 bits (82), Expect = 0.003
 Identities = 20/56 (35%), Positives = 30/56 (53%)
 Frame = +3

Query: 225 ETFDDMNLKEELLRGIYAYGFEKPSAIQQRAIMPWHPRDAMLSLKAQSGTGKNCYF 392
           +TF D NL   L R I+   FEKP+++Q   I P       L  +A++G+GK   +
Sbjct: 14  KTFSDFNLDPRLQRAIHKCEFEKPTSVQSETI-PLALEGKDLVAQARTGSGKTAAY 68


>SPAC1F7.02c |||ATP-dependent RNA helicase Has1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 578

 Score = 36.3 bits (80), Expect = 0.006
 Identities = 21/66 (31%), Positives = 34/66 (51%)
 Frame = +3

Query: 195 HLTPTGDQVVETFDDMNLKEELLRGIYAYGFEKPSAIQQRAIMPWHPRDAMLSLKAQSGT 374
           +L  +    +E F D+ L E + + I   GFE  + IQ+R+I P      +L   A++G+
Sbjct: 79  NLNASSTSDIEKFSDLQLSENIQKAIKEMGFETMTEIQKRSIPPLLAGRDVLG-AAKTGS 137

Query: 375 GKNCYF 392
           GK   F
Sbjct: 138 GKTLAF 143


>SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 535

 Score = 34.3 bits (75), Expect = 0.024
 Identities = 16/42 (38%), Positives = 24/42 (57%)
 Frame = +3

Query: 282 GFEKPSAIQQRAIMPWHPRDAMLSLKAQSGTGKNCYFSLYRF 407
           GFEK + +QQR +    P +    ++A++GTGK   F L  F
Sbjct: 57  GFEKMTPVQQRVLNEVFPNEENAVVQAKTGTGKTLAFLLVAF 98


>SPAC30D11.03 |drs1||ATP-dependent RNA helicase Drs1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 754

 Score = 32.7 bits (71), Expect = 0.072
 Identities = 19/55 (34%), Positives = 28/55 (50%)
 Frame = +3

Query: 228 TFDDMNLKEELLRGIYAYGFEKPSAIQQRAIMPWHPRDAMLSLKAQSGTGKNCYF 392
           +F  MNL   +L+G+   GFE P+ IQ + I P       +   A +G+GK   F
Sbjct: 260 SFQSMNLSRPILKGLSNLGFEVPTQIQDKTI-PLALLGKDIVGAAVTGSGKTAAF 313


>SPBP8B7.16c |dbp2||ATP-dependent RNA helicase
           Dbp2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 550

 Score = 31.9 bits (69), Expect = 0.13
 Identities = 21/60 (35%), Positives = 33/60 (55%), Gaps = 1/60 (1%)
 Frame = +3

Query: 222 VETFDDMNLKEELLRGIYAYGFEKPSAIQQRA-IMPWHPRDAMLSLKAQSGTGKNCYFSL 398
           V TF++      +L+ +   GFE P+ IQQ+A  M    RD M+ + A +G+GK   + L
Sbjct: 121 VTTFEEAGFPNYVLKEVKQLGFEAPTPIQQQAWPMAMSGRD-MVGISA-TGSGKTLSYCL 178


>SPCP1E11.03 |mug170||arrestin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 426

 Score = 29.9 bits (64), Expect = 0.51
 Identities = 19/84 (22%), Positives = 39/84 (46%), Gaps = 9/84 (10%)
 Frame = -3

Query: 343 ASLGCQGIIARCWIAEGFS---------KP*AYMPLNNSSLRFMSSKVSTT*SPVGVKCP 191
           AS+G +G+  +C    GF           P  Y       L ++SS ++ T     ++C 
Sbjct: 172 ASIGNKGLFLKCSCKVGFPFSFIVPLDIGPGTYHSSKLELLYYISSTLTLTSLDQNIRCT 231

Query: 190 RVHSRRSIVTTLDPSMAHFWNPLA 119
           R    + ++T++  ++A  +NP++
Sbjct: 232 RCTIPKRVITSMHNNIAELYNPIS 255


>SPCC10H11.01 |prp11||ATP-dependent RNA helicase
           Prp11|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1014

 Score = 29.1 bits (62), Expect = 0.88
 Identities = 18/62 (29%), Positives = 33/62 (53%)
 Frame = +3

Query: 222 VETFDDMNLKEELLRGIYAYGFEKPSAIQQRAIMPWHPRDAMLSLKAQSGTGKNCYFSLY 401
           V ++    L  + +  I + G+EKP++IQ +AI        ++ + A++G+GK   F L 
Sbjct: 417 VTSWSQCGLSAQTISVINSLGYEKPTSIQAQAIPAITSGRDVIGV-AKTGSGKTIAFLLP 475

Query: 402 RF 407
            F
Sbjct: 476 MF 477


>SPCC777.06c |||hydrolase |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 301

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 10/13 (76%), Positives = 11/13 (84%)
 Frame = +3

Query: 171 DLREWTLGHLTPT 209
           DLREWTLG L P+
Sbjct: 110 DLREWTLGFLQPS 122


>SPBC530.12c |pdf1||palmitoyl protein thioesterase-dolichol
           pyrophosphate phosphatase fusion 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 603

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 17/64 (26%), Positives = 31/64 (48%)
 Frame = +3

Query: 102 KKVRRLARGFQKWAIEGSRVVTMDLREWTLGHLTPTGDQVVETFDDMNLKEELLRGIYAY 281
           ++V++L +G    A+   RV   +  +   G+L    DQ+ E  D +  ++ L  G YA 
Sbjct: 48  QRVQKLTKG----AVYAIRVGDNEFEDIKAGYLGKLEDQLDEVCDLIGNEDSLSNGFYAL 103

Query: 282 GFEK 293
           G  +
Sbjct: 104 GLSQ 107


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,684,302
Number of Sequences: 5004
Number of extensions: 77489
Number of successful extensions: 175
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)