BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP16_F_M04
(870 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 31 0.28
SPAC821.08c |slp1||sleepy homolog Slp1|Schizosaccharomyces pombe... 27 2.6
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 27 3.5
SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po... 27 3.5
SPBC660.05 |||conserved fungal protein|Schizosaccharomyces pombe... 26 6.1
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 23 6.7
SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 26 8.0
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 30.7 bits (66), Expect = 0.28
Identities = 17/49 (34%), Positives = 27/49 (55%)
Frame = +3
Query: 171 YDPFSPYVRESMLDTHSLWSNLANEMQHLDDMMKELSLKFPSIINEGRV 317
Y+ P ++S + +L S L LDD+ K L+L FP++I + RV
Sbjct: 153 YELPDPETKKSKIK--ALQSQLVRVNGELDDLQKHLTLSFPTLIAKSRV 199
>SPAC821.08c |slp1||sleepy homolog Slp1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 488
Score = 27.5 bits (58), Expect = 2.6
Identities = 12/49 (24%), Positives = 25/49 (51%)
Frame = +3
Query: 636 LTSAWRQPRRPMRSPKAVXATTYAVXIRDDAGVLADSHINLIQIXNVRI 782
L + +P+RP+ +P T + D G++ D ++NL+ N+ +
Sbjct: 145 LRTQHNRPQRPVVTPAKRRFNTTPERVLDAPGIIDDYYLNLLDWSNLNV 193
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.1 bits (57), Expect = 3.5
Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +1
Query: 124 PRHSTTMARHIGRITITT-PSVLTFGKACWTHI 219
P H+TT R I +I I PS+LT G +C HI
Sbjct: 553 PVHATT--RFIAQIAILELPSILTTGYSCVMHI 583
>SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 851
Score = 27.1 bits (57), Expect = 3.5
Identities = 16/65 (24%), Positives = 31/65 (47%)
Frame = +3
Query: 198 ESMLDTHSLWSNLANEMQHLDDMMKELSLKFPSIINEGRVEGDKYQISIHLPGYEQKDIN 377
E + + +W+++ +MQ D ++L F I N G++ Q +I L G + ++
Sbjct: 104 EGSTNVNEVWNDITEDMQSQDFSTEDLKQLFLLIFNNGKLR-TLLQNAIVLLGQQTTNVA 162
Query: 378 VKAKN 392
K N
Sbjct: 163 SKKLN 167
>SPBC660.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 143
Score = 26.2 bits (55), Expect = 6.1
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = -2
Query: 866 GXGGXGGGXXGGXGG 822
G GG GGG GG GG
Sbjct: 125 GFGGRGGGRMGGRGG 139
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 22.6 bits (46), Expect(2) = 6.7
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +1
Query: 832 PPXXPPPXPP 861
PP PPP PP
Sbjct: 20 PPSQPPPPPP 29
Score = 21.4 bits (43), Expect(2) = 6.7
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = +1
Query: 823 PPXPPXXPPPXP 858
PP P PPP P
Sbjct: 5 PPGNPPPPPPPP 16
>SPCC1450.11c |cek1||serine/threonine protein kinase
Cek1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1338
Score = 25.8 bits (54), Expect = 8.0
Identities = 23/77 (29%), Positives = 39/77 (50%), Gaps = 7/77 (9%)
Frame = +3
Query: 216 HSLWSNLANEMQHLDD--MMKELSL-KFPSI-INEGRVEGDKYQISIHLPGY---EQKDI 374
H++ N A E ++D ++K +S F S+ + + R GD + I I + + I
Sbjct: 573 HTIVPNSAPEHPSINDYKILKPISKGAFGSVYLAQKRTTGDYFAIKILKKSNMIAKNQVI 632
Query: 375 NVKAKNGVLMVQANSAF 425
NV+A+ +LM Q S F
Sbjct: 633 NVRAERAILMSQGESPF 649
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,211,038
Number of Sequences: 5004
Number of extensions: 62130
Number of successful extensions: 235
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 434475230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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