Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP16_F_L06
(911 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 1.2
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 2.8
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 3.5
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.9
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 4.9
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 6.5
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 6.5
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 6.5
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 8.5
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 8.5
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 26 8.5
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.7 bits (61), Expect = 1.2
Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 2/51 (3%)
Frame = +1
Query: 739 PPXPPPXPPPXPXXGSRPXAGLXXQXPVXFGIP--XPXXHLLPXXXPAQPP 885
PP PP PP G+ L P+ +P P LP PA PP
Sbjct: 428 PPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPP 478
Score = 27.1 bits (57), Expect = 3.7
Identities = 21/60 (35%), Positives = 23/60 (38%), Gaps = 2/60 (3%)
Frame = +1
Query: 736 PPPXPPPXPPPXPXXGSRPXAGLXXQXPVXFGIP--XPXXHLLPXXXPAQPPTXNARRXP 909
PP PP PP P S P + L P +P P LP PA PP A P
Sbjct: 420 PPSLPPSAPPSLPP--SAPPS-LPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAP 476
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 27.5 bits (58), Expect = 2.8
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +1
Query: 736 PPPXPPPXPPPXPXXGSRP 792
P P P P PPP P G P
Sbjct: 744 PAPAPIPVPPPAPIMGGPP 762
Score = 27.1 bits (57), Expect = 3.7
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +1
Query: 739 PPXPPPXPPPXPXXGSR 789
PP PPP PP GSR
Sbjct: 775 PPPPPPPPPAVSAGGSR 791
Score = 26.2 bits (55), Expect = 6.5
Identities = 20/65 (30%), Positives = 22/65 (33%), Gaps = 6/65 (9%)
Frame = +1
Query: 721 LXLXXPPPXPP------PXPPPXPXXGSRPXAGLXXQXPVXFGIPXPXXHLLPXXXPAQP 882
L L PPP PP P P P P P G P G+ P P P
Sbjct: 727 LLLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAG----PPPPPPPP 782
Query: 883 PTXNA 897
P +A
Sbjct: 783 PAVSA 787
Score = 25.8 bits (54), Expect = 8.5
Identities = 16/47 (34%), Positives = 20/47 (42%), Gaps = 3/47 (6%)
Frame = +1
Query: 661 PPPXQ*PVXIRXXPTLXKYXLXLXXPPPX---PPPXPPPXPXXGSRP 792
PPP V + PT + + P P PPP PPP G+ P
Sbjct: 732 PPPPPPAVIV---PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGP 775
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.2 bits (55), Expect = 6.5
Identities = 17/59 (28%), Positives = 22/59 (37%)
Frame = +1
Query: 727 LXXPPPXPPPXPPPXPXXGSRPXAGLXXQXPVXFGIPXPXXHLLPXXXPAQPPTXNARR 903
L PPP PPP PP S+ + + P P P +PPT + R
Sbjct: 235 LPAPPPPPPPTLPPQSTNTSQLPMPSRNVNNLGSQVNIPPPPATP-SQPPRPPTNASTR 292
Score = 23.0 bits (47), Expect(2) = 3.5
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +1
Query: 739 PPXPPPXPP 765
PP PPP PP
Sbjct: 189 PPPPPPPPP 197
Score = 23.0 bits (47), Expect(2) = 9.6
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +1
Query: 742 PXPPPXPPP 768
P PPP PPP
Sbjct: 189 PPPPPPPPP 197
Score = 22.2 bits (45), Expect(2) = 3.5
Identities = 7/11 (63%), Positives = 7/11 (63%)
Frame = +1
Query: 742 PXPPPXPPPXP 774
P P P PPP P
Sbjct: 234 PLPAPPPPPPP 244
Score = 20.6 bits (41), Expect(2) = 9.6
Identities = 9/25 (36%), Positives = 11/25 (44%)
Frame = +1
Query: 748 PPPXPPPXPXXGSRPXAGLXXQXPV 822
P P PPP P P + Q P+
Sbjct: 234 PLPAPPPPPPPTLPPQSTNTSQLPM 258
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.9
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +1
Query: 175 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 294
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 4.9
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +1
Query: 169 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 258
++ DFFK + ++ H TL ++ NSL+
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 26.2 bits (55), Expect = 6.5
Identities = 19/64 (29%), Positives = 29/64 (45%)
Frame = +1
Query: 82 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 261
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 262 SKDA 273
SKDA
Sbjct: 230 SKDA 233
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 26.2 bits (55), Expect = 6.5
Identities = 15/37 (40%), Positives = 15/37 (40%)
Frame = -1
Query: 773 GXGGGXGGGXGGGXXRXRXYFXXVGXXRIXTGHXFGG 663
G GGG GG GGG R G R G GG
Sbjct: 27 GFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGG 63
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 6.5
Identities = 14/50 (28%), Positives = 23/50 (46%)
Frame = +1
Query: 211 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGSA 360
+K+ + E+Q +T DFS K + L+AF K L+ S+
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASS 138
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 25.8 bits (54), Expect = 8.5
Identities = 14/47 (29%), Positives = 18/47 (38%), Gaps = 3/47 (6%)
Frame = +1
Query: 661 PPPXQ*PVXIRXXPTLXKYXLXLXXPPPXPPPXP---PPXPXXGSRP 792
PPP Q ++ P+ + PP PP P P SRP
Sbjct: 172 PPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPPLSQAPVANTSSRP 218
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 8.5
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +1
Query: 139 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 315
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 316 LQQL 327
Q+
Sbjct: 73 NNQI 76
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 25.8 bits (54), Expect = 8.5
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = -1
Query: 818 GXXXXRPAXGREPXXGXGGGXGGGXGGG 735
G R G G GGG GG GGG
Sbjct: 146 GRGGSRGGFGGNSRGGFGGGSRGGFGGG 173
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,460,911
Number of Sequences: 5004
Number of extensions: 40872
Number of successful extensions: 251
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 462505890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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