BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP16_F_K06
(914 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 2.8
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 3.7
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 27 3.7
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 4.9
SPAC821.08c |slp1||sleepy homolog Slp1|Schizosaccharomyces pombe... 27 4.9
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 23 7.4
SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy... 26 8.6
SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizo... 26 8.6
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.5 bits (58), Expect = 2.8
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +3
Query: 702 PLXPXPPXXPPTPPPXPPXXPLP 770
PL P P PP P P PLP
Sbjct: 448 PLPPSAPIAPPLPAGMPAAPPLP 470
Score = 27.5 bits (58), Expect = 2.8
Identities = 10/23 (43%), Positives = 11/23 (47%)
Frame = +3
Query: 702 PLXPXPPXXPPTPPPXPPXXPLP 770
P+ P P P PP PP P P
Sbjct: 454 PIAPPLPAGMPAAPPLPPAAPAP 476
Score = 26.6 bits (56), Expect = 4.9
Identities = 11/22 (50%), Positives = 12/22 (54%), Gaps = 1/22 (4%)
Frame = +3
Query: 702 PLXPXPPXXPPT-PPPXPPXXP 764
P P PP PP+ PP PP P
Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAP 436
Score = 26.6 bits (56), Expect = 4.9
Identities = 12/25 (48%), Positives = 12/25 (48%), Gaps = 2/25 (8%)
Frame = +3
Query: 702 PLXPXPPXXPPTPP--PXPPXXPLP 770
PL P PP PP P PP P P
Sbjct: 458 PLPAGMPAAPPLPPAAPAPPPAPAP 482
Score = 23.8 bits (49), Expect(2) = 6.3
Identities = 8/15 (53%), Positives = 8/15 (53%)
Frame = +3
Query: 711 PXPPXXPPTPPPXPP 755
P PP PPP PP
Sbjct: 352 PLPPQGRSAPPPPPP 366
Score = 20.6 bits (41), Expect(2) = 6.3
Identities = 7/14 (50%), Positives = 7/14 (50%)
Frame = +3
Query: 729 PPTPPPXPPXXPLP 770
PP PPP P P
Sbjct: 388 PPAPPPAIPGRSAP 401
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.1 bits (57), Expect = 3.7
Identities = 13/31 (41%), Positives = 15/31 (48%), Gaps = 2/31 (6%)
Frame = +3
Query: 702 PLXPXPPXXPPT--PPPXPPXXPLPRKHXRP 788
P P P PP+ PPP PP RK+ P
Sbjct: 11 PPPPPPGFEPPSQPPPPPPPGYVKKRKNKTP 41
Score = 26.2 bits (55), Expect = 6.5
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +3
Query: 717 PPXXPPTPPPXPPXXP 764
PP PP PPP P P
Sbjct: 5 PPGNPPPPPPPPGFEP 20
Score = 26.2 bits (55), Expect = 6.5
Identities = 12/25 (48%), Positives = 12/25 (48%), Gaps = 2/25 (8%)
Frame = +3
Query: 702 PLXPXPPXXPP--TPPPXPPXXPLP 770
P P PP PP PP PP P P
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 27.1 bits (57), Expect = 3.7
Identities = 18/51 (35%), Positives = 24/51 (47%)
Frame = +1
Query: 394 CSXCSIMPKILXVSTKXHATPEST*TXXCSYTPTTXLLSSASDPAQLRSTW 546
CS S+ P + +TK T T T + TPTT +S + AQ S W
Sbjct: 922 CSDGSLSPNTV-TTTKATTTFTPTPTTTTTPTPTTTSATSTNVIAQCGSAW 971
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 4.9
Identities = 12/31 (38%), Positives = 12/31 (38%)
Frame = +3
Query: 702 PLXPXPPXXPPTPPPXPPXXPLPRKHXRPXL 794
P P PP P PP PP P P L
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPL 1735
>SPAC821.08c |slp1||sleepy homolog Slp1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 488
Score = 26.6 bits (56), Expect = 4.9
Identities = 17/62 (27%), Positives = 31/62 (50%), Gaps = 1/62 (1%)
Frame = -1
Query: 338 GKESDLVHHHEIXVGFHVCVAVLAGLDVEVLGDFVVLSFIADLVNVVE-KRQNLLLLFDE 162
G S +HHH++ + H + L G EV G L++ +D + + N++ ++D
Sbjct: 279 GSRSGAIHHHDVRIANHQ-IGTLQGHSSEVCG----LAWRSDGLQLASGGNDNVVQIWDA 333
Query: 161 RS 156
RS
Sbjct: 334 RS 335
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 23.4 bits (48), Expect(2) = 7.4
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +3
Query: 702 PLXPXPPXXPPTPPPXPPXXPLP 770
P P P PTP P P P P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPP 754
Score = 20.6 bits (41), Expect(2) = 7.4
Identities = 7/15 (46%), Positives = 7/15 (46%)
Frame = +3
Query: 867 PXXPTXXXPXXPPPP 911
P P P PPPP
Sbjct: 753 PPAPIMGGPPPPPPP 767
>SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 603
Score = 25.8 bits (54), Expect = 8.6
Identities = 15/52 (28%), Positives = 28/52 (53%)
Frame = +3
Query: 102 LPSLSASTVPEFKTTPVDAAFVEKQKKILSLFYNVNEISYEAEYYKVAQDFN 257
LPS ++S+VP+ +T+ + + +I SL N E+S ++ Q +N
Sbjct: 86 LPSATSSSVPQSRTSELSSTNSIPTPRITSL--NPEELSRRERAQRLLQTYN 135
>SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 944
Score = 25.8 bits (54), Expect = 8.6
Identities = 17/46 (36%), Positives = 21/46 (45%)
Frame = -2
Query: 169 STNAASTGVVLNSGTVLAESEGNKPR*NQDSLHGLSRRSELSNLKE 32
STNA S + GTV A G +P S S + LSNL +
Sbjct: 72 STNAQSNIATPSPGTVAAAISGIQPPPKTPSSDSPSLENSLSNLND 117
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,618,378
Number of Sequences: 5004
Number of extensions: 44076
Number of successful extensions: 219
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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