Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP16_F_J02
(882 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1685.10 |rps27||40S ribosomal protein S27|Schizosaccharomyce... 139 6e-34
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 36 0.010
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 35 0.018
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 33 0.054
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.054
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 24 0.93
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 28 1.5
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 28 1.5
SPAC30D11.14c |||RNA-binding protein |Schizosaccharomyces pombe|... 28 1.5
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 28 2.0
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 8.2
>SPBC1685.10 |rps27||40S ribosomal protein S27|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 83
Score = 139 bits (336), Expect = 6e-34
Identities = 60/82 (73%), Positives = 70/82 (85%)
Frame = +2
Query: 86 MPLAIDLLHPSPASERRKHKLKRLVPHPNSYFMDVKCPGCYKITTVFSHAQRVVVCAGCS 265
M LA+DLL+PS SE RKHKLK+LV P S+FMDVKCPGC+ ITTVFSHAQ VV+C C+
Sbjct: 1 MVLAVDLLNPSHESEMRKHKLKQLVQGPRSFFMDVKCPGCFNITTVFSHAQTVVICGSCA 60
Query: 266 TILCQPTGGRARLTEGCSFRRK 331
++LCQPTGG+ARL EGCSFRRK
Sbjct: 61 SVLCQPTGGKARLMEGCSFRRK 82
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 35.5 bits (78), Expect = 0.010
Identities = 12/18 (66%), Positives = 12/18 (66%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPP 530
PPPP PP PPPP PP
Sbjct: 13 PPPPGFEPPSQPPPPPPP 30
Score = 33.5 bits (73), Expect = 0.041
Identities = 14/27 (51%), Positives = 14/27 (51%)
Frame = +3
Query: 468 GKXPPPPXXPPPXXPPPPXPPXXXXPG 548
G PPPP PPP PP PP PG
Sbjct: 7 GNPPPPP--PPPGFEPPSQPPPPPPPG 31
Score = 31.1 bits (67), Expect = 0.22
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +3
Query: 495 PPPXXPPPPXPPXXXXPGAGXPPXXEXY 578
PP PPPP PP P PP Y
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPPGY 32
Score = 27.9 bits (59), Expect = 2.0
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +3
Query: 483 PPXXPPPXXPPPPXPPXXXXPGAGXP 560
PP PPP PPP P P P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 34.7 bits (76), Expect = 0.018
Identities = 25/71 (35%), Positives = 27/71 (38%), Gaps = 1/71 (1%)
Frame = +3
Query: 432 SI*ALQIKAFSKGKXP-PPPXXPPPXXPPPPXPPXXXXPGAGXPPXXEXYXAPXPX*NXW 608
S+ A + A S G P P P P PPP P P G PP AP P
Sbjct: 1165 SVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAP-PVPTPS 1223
Query: 609 HXLXPPPXXTA 641
L P P TA
Sbjct: 1224 AGLPPVPVPTA 1234
Score = 27.9 bits (59), Expect = 2.0
Identities = 17/52 (32%), Positives = 18/52 (34%), Gaps = 1/52 (1%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPPXXEXYXA-PXPX*NXWHXLXPPP 629
P P PP P P P AG PP A P P + PPP
Sbjct: 1162 PKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPP 1213
Score = 26.6 bits (56), Expect = 4.7
Identities = 31/131 (23%), Positives = 38/131 (29%), Gaps = 4/131 (3%)
Frame = +3
Query: 462 SKGKXPPPPXXPPPXXPPPPXPPXXXXP----GAGXPPXXEXYXAPXPX*NXWHXLXPPP 629
S G PP P P P P P +G PP + A P + P P
Sbjct: 1056 SSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVP 1115
Query: 630 XXTAGKKXLFLXXXRPLXPLXVPXXNPXQPX*XHXXASIXSSPXXAXVXXPPXXXPXKXN 809
+ P P+ VP P P S+ + P A PP P
Sbjct: 1116 APSGAPPVPKPSVAAP--PVPVPSGAPPVP-----KPSVAAPPVPAPSGAPPVPKPSVAA 1168
Query: 810 SPHXPPRXQXP 842
P P P
Sbjct: 1169 PPVPAPSSGIP 1179
Score = 26.2 bits (55), Expect = 6.2
Identities = 35/145 (24%), Positives = 39/145 (26%), Gaps = 1/145 (0%)
Frame = +3
Query: 450 IKAFSKGKXPPPPXXPPPXXPPPPXPPXXXXPGAGXPPXXEXYXAPXPX*NXWHXLXPPP 629
I A S P P PP P P P PP AP P P P
Sbjct: 1076 IPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAP-PVPKPSVAAPPVP 1134
Query: 630 XXTAGKKXLFLXXXRPLXPLXVPXXNPXQPX*XHXXASIXSSPXXA-XVXXPPXXXPXKX 806
+ P P+ P P P S+ + P A PP P
Sbjct: 1135 VPSGAPPVPKPSVAAP--PVPAPSGAPPVP-----KPSVAAPPVPAPSSGIPPVPKPAAG 1187
Query: 807 NSPHXPPRXQXPXGFLGXXFPXPPP 881
P PP P P PP
Sbjct: 1188 VPPVPPPSEAPPVPKPSVGVPPVPP 1212
Score = 25.8 bits (54), Expect = 8.2
Identities = 14/35 (40%), Positives = 14/35 (40%), Gaps = 1/35 (2%)
Frame = +3
Query: 462 SKGKXP-PPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
S G P PPP PP P P P A PP
Sbjct: 1204 SVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPP 1238
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 33.1 bits (72), Expect = 0.054
Identities = 14/37 (37%), Positives = 15/37 (40%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPPXXEXYXAP 587
P PP P PPP PP P + PP AP
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/30 (43%), Positives = 13/30 (43%), Gaps = 1/30 (3%)
Frame = +3
Query: 477 PPPPXXPP-PXXPPPPXPPXXXXPGAGXPP 563
PPPP PP P PP PP A P
Sbjct: 1715 PPPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744
Score = 27.5 bits (58), Expect = 2.7
Identities = 20/67 (29%), Positives = 20/67 (29%)
Frame = +3
Query: 441 ALQIKAFSKGKXPPPPXXPPPXXPPPPXPPXXXXPGAGXPPXXEXYXAPXPX*NXWHXLX 620
AL K F G P P PP P PP P PP P
Sbjct: 1672 ALAAKLFG-GMAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSA 1730
Query: 621 PPPXXTA 641
PPP A
Sbjct: 1731 PPPPLPA 1737
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 33.1 bits (72), Expect = 0.054
Identities = 15/29 (51%), Positives = 15/29 (51%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
P PP P PPPP PP GAG PP
Sbjct: 750 PVPPPAPIMGGPPPP-PPPPGVAGAGPPP 777
Score = 30.7 bits (66), Expect = 0.29
Identities = 15/39 (38%), Positives = 15/39 (38%)
Frame = +3
Query: 447 QIKAFSKGKXPPPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
Q K K PPPP P P P P P G PP
Sbjct: 724 QQKLLLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPP 762
Score = 30.7 bits (66), Expect = 0.29
Identities = 17/42 (40%), Positives = 17/42 (40%), Gaps = 4/42 (9%)
Frame = +3
Query: 480 PPPXXPPPXX----PPPPXPPXXXXPGAGXPPXXEXYXAPXP 593
PPP PPP PPPP PP G Y AP P
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPAVSAGG-----SRYYAPAP 797
Score = 26.6 bits (56), Expect = 4.7
Identities = 17/48 (35%), Positives = 19/48 (39%)
Frame = +3
Query: 738 ASIXSSPXXAXVXXPPXXXPXKXNSPHXPPRXQXPXGFLGXXFPXPPP 881
A I +P A + PP P P PP P G G P PPP
Sbjct: 738 AVIVPTPAPAPIPVPPPA-PIMGGPPPPPP----PPGVAGAGPPPPPP 780
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 24.2 bits (50), Expect(2) = 0.93
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +3
Query: 480 PPPXXPPPXXPPPPXPP 530
P P PPP PPP PP
Sbjct: 234 PLPAPPPP--PPPTLPP 248
Score = 23.0 bits (47), Expect(2) = 0.93
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +3
Query: 477 PPPPXXPPP 503
PPPP PPP
Sbjct: 189 PPPPPPPPP 197
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
PPPP P PP PP PG PP
Sbjct: 208 PPPPMHHKPGEHMPP-PPMHHEPGEHMPP 235
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
PPPP P PP PP PG PP
Sbjct: 221 PPPPMHHEPGEHMPP-PPMHHEPGEHMPP 248
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
PPPP P PP PP PG PP
Sbjct: 234 PPPPMHHEPGEHMPP-PPMHHEPGEHMPP 261
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
PPPP P PP PP PG PP
Sbjct: 247 PPPPMHHEPGEHMPP-PPMHHEPGEHMPP 274
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
PPPP P PP PP PG PP
Sbjct: 260 PPPPMHHEPGEHMPP-PPMHHEPGEHMPP 287
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
PPPP P PP PP PG PP
Sbjct: 273 PPPPMHHEPGEHMPP-PPMHHEPGEHMPP 300
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
PPPP P PP PP PG PP
Sbjct: 286 PPPPMHHEPGEHMPP-PPMHHEPGEHMPP 313
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
PPPP P PP PP PG PP
Sbjct: 299 PPPPMHHEPGEHMPP-PPMHHEPGEHMPP 326
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
PPPP P PP PP PG PP
Sbjct: 312 PPPPMHHEPGEHMPP-PPMHHEPGEHMPP 339
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 28.3 bits (60), Expect = 1.5
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +3
Query: 477 PPPPXXPPPXXPPPPXPPXXXXPGAGXP 560
PPPP P PP P P AG P
Sbjct: 1883 PPPPPMALPKAGPPSAAPTSALPPAGPP 1910
>SPAC30D11.14c |||RNA-binding protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 28.3 bits (60), Expect = 1.5
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +3
Query: 498 PPXXPPPPXPPXXXXPGAGXPP 563
PP P PP PP P PP
Sbjct: 129 PPSAPAPPLPPSSEGPAVDIPP 150
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 331
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +3
Query: 480 PPPXXPPPXXPPPPXPPXXXXPGAGXPP 563
P P P P PP P P P G PP
Sbjct: 113 PVPEEPLPGEPPLPDEPVPEEPLPGEPP 140
Score = 25.8 bits (54), Expect = 8.2
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +3
Query: 486 PXXPPPXXPPPPXPPXXXXPGAGXPP 563
P P P PP P P P G PP
Sbjct: 99 PEEPLPREPPLPNEPVPEEPLPGEPP 124
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 25.8 bits (54), Expect = 8.2
Identities = 14/45 (31%), Positives = 17/45 (37%), Gaps = 1/45 (2%)
Frame = +3
Query: 462 SKGKXPPPPXXPPPXXPPPPXPPXXXXPGA-GXPPXXEXYXAPXP 593
++GK P PPPP PP G+ PP P P
Sbjct: 321 NRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPP 365
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,898,951
Number of Sequences: 5004
Number of extensions: 52724
Number of successful extensions: 351
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 225
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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