Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP16_F_I23
(872 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 54 2e-08
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 51 2e-07
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 46 9e-06
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.010
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 34 0.030
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 26 6.1
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 54.4 bits (125), Expect = 2e-08
Identities = 31/87 (35%), Positives = 46/87 (52%)
Frame = +1
Query: 199 FQEAFQLFDSRGDGKIHVAXIGDALXALGQNPTXSDVKKCTLHLKPDERISFEVFLPIYQ 378
+++AF LFD G G+I IGD L A GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 379 AISKARSGDTANDFIEGLRHFDKMAMG 459
+ +F++G + FDK A G
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATG 92
Score = 25.8 bits (54), Expect = 8.1
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +1
Query: 196 EFQEAFQLFDSRGDGKIHVAXIGDALXALGQ 288
EF + FQ+FD G I V + L +LG+
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGE 108
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 50.8 bits (116), Expect = 2e-07
Identities = 31/96 (32%), Positives = 49/96 (51%), Gaps = 2/96 (2%)
Frame = +1
Query: 178 SEDQLAEFQEAFQLFDSRGDGKIHVAXIGDALXALGQNPTXSDVKKCTLHLKPDER--IS 351
+++Q+AEF+EAF LFD DG I +G + +LGQ+PT ++++ + D I
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 352 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKMAMG 459
F FL + K + D + E + FDK G
Sbjct: 67 FTEFLTM--MARKMKDTDNEEEVREAFKVFDKDGNG 100
Score = 31.1 bits (67), Expect = 0.21
Identities = 15/42 (35%), Positives = 24/42 (57%)
Frame = +1
Query: 184 DQLAEFQEAFQLFDSRGDGKIHVAXIGDALXALGQNPTXSDV 309
D E +EAF++FD G+G I V + L +LG+ + +V
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEV 123
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 45.6 bits (103), Expect = 9e-06
Identities = 28/90 (31%), Positives = 48/90 (53%)
Frame = +1
Query: 178 SEDQLAEFQEAFQLFDSRGDGKIHVAXIGDALXALGQNPTXSDVKKCTLHLKPDERISFE 357
S++Q E +EAF L+D DG I + +G L +LG N T +++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 358 VFLPIYQAISKARSGDTANDFIEGLRHFDK 447
F+ +K R ++ ++I+ R FDK
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDK 89
Score = 32.3 bits (70), Expect = 0.093
Identities = 16/65 (24%), Positives = 32/65 (49%)
Frame = +1
Query: 196 EFQEAFQLFDSRGDGKIHVAXIGDALXALGQNPTXSDVKKCTLHLKPDERISFEVFLPIY 375
E+ +AF++FD G I A D + LG+ + ++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 376 QAISK 390
+ ++K
Sbjct: 139 RIMAK 143
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.5 bits (78), Expect = 0.010
Identities = 19/46 (41%), Positives = 26/46 (56%)
Frame = +1
Query: 178 SEDQLAEFQEAFQLFDSRGDGKIHVAXIGDALXALGQNPTXSDVKK 315
+E+Q + EAF+LFDS D I + A+ ALG N S+V K
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLK 77
Score = 27.5 bits (58), Expect = 2.6
Identities = 18/65 (27%), Positives = 29/65 (44%), Gaps = 2/65 (3%)
Frame = +1
Query: 184 DQLAEFQEAFQLFDSRGDGKIHVAXIGDALXALGQNPTXSDVKKC--TLHLKPDERISFE 357
D L E + AF+LFD GKI + + L +N +++ L D I+ +
Sbjct: 107 DPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQ 166
Query: 358 VFLPI 372
F+ I
Sbjct: 167 EFIAI 171
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 33.9 bits (74), Expect = 0.030
Identities = 26/97 (26%), Positives = 39/97 (40%)
Frame = +1
Query: 169 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAXIGDALXALGQNPTXSDVKKCTLHLKPDERI 348
A + Q+ E +EAF L D GDG I + L +L Q+ + + + P I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--I 97
Query: 349 SFEVFLPIYQAISKARSGDTANDFIEGLRHFDKMAMG 459
+ FL ++ S ND +E FD G
Sbjct: 98 NLAAFLTAMGSMLCRIS--PRNDLLEAFSTFDDTQSG 132
Score = 29.5 bits (63), Expect = 0.65
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 205 EAFQLFDSRGDGKIHVAXIGDALXALGQNPTXSDVK 312
EAF FD GKI ++ + DAL ++G +V+
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVE 156
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 26.2 bits (55), Expect = 6.1
Identities = 18/64 (28%), Positives = 29/64 (45%)
Frame = +1
Query: 196 EFQEAFQLFDSRGDGKIHVAXIGDALXALGQNPTXSDVKKCTLHLKPDERISFEVFLPIY 375
E +EAF LFD G I + + LG+N T ++ + ++S E F ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAELW 72
Query: 376 QAIS 387
IS
Sbjct: 73 IHIS 76
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,776,256
Number of Sequences: 5004
Number of extensions: 47923
Number of successful extensions: 122
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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