Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP16_F_I13
         (838 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|...   122   8e-29
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase...   119   4e-28

>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
           Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score =  122 bits (293), Expect = 8e-29
 Identities = 58/85 (68%), Positives = 70/85 (82%)
 Frame = +1

Query: 304 LLVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 483
           L+++G+ I V +ERDP  I W  +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAPS 
Sbjct: 67  LVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAPSK 126

Query: 484 DAPMXVVGVNLXAYDPSFKVISNAS 558
           DAPM VVGVNL  ++PS KVISNAS
Sbjct: 127 DAPMFVVGVNLEKFNPSEKVISNAS 151



 Score = 98.3 bits (234), Expect = 1e-21
 Identities = 46/63 (73%), Positives = 53/63 (84%), Gaps = 1/63 (1%)
 Frame = +3

Query: 120 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 296
           K+GINGFGRIGR+VLR +I  G  QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + 
Sbjct: 5   KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64

Query: 297 GFL 305
           G L
Sbjct: 65  GKL 67



 Score = 44.0 bits (99), Expect = 3e-05
 Identities = 32/79 (40%), Positives = 35/79 (44%)
 Frame = +3

Query: 576 LPHLAKVIHDNFEIVEGLMXXXXXXXXXXXXXDGPSXKLWXDAPWCSTKHHSXPXLGAAQ 755
           L  LAKVI+D F I EGLM             DGPS K W      S  +      GAA+
Sbjct: 157 LAPLAKVINDTFGIEEGLMTTVHATTATQKTVDGPSKKDWRGGRGASA-NIIPSSTGAAK 215

Query: 756 XCGXXYPLPPNGTLTGMPF 812
             G   P   NG LTGM F
Sbjct: 216 AVGKVIP-ALNGKLTGMAF 233


>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
           Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 336

 Score =  119 bits (287), Expect = 4e-28
 Identities = 57/85 (67%), Positives = 69/85 (81%)
 Frame = +1

Query: 304 LLVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 483
           L+++GN I V +ERDP  I W  +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAPS 
Sbjct: 67  LVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAPSK 126

Query: 484 DAPMXVVGVNLXAYDPSFKVISNAS 558
           DAPM VVGVN   ++PS KVISNAS
Sbjct: 127 DAPMYVVGVNEEKFNPSEKVISNAS 151



 Score =  101 bits (241), Expect = 2e-22
 Identities = 47/63 (74%), Positives = 54/63 (85%), Gaps = 1/63 (1%)
 Frame = +3

Query: 120 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 296
           K+GINGFGRIGR+VLR A + K  QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D
Sbjct: 5   KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64

Query: 297 GFL 305
           G L
Sbjct: 65  GKL 67



 Score = 44.0 bits (99), Expect = 3e-05
 Identities = 32/79 (40%), Positives = 35/79 (44%)
 Frame = +3

Query: 576 LPHLAKVIHDNFEIVEGLMXXXXXXXXXXXXXDGPSXKLWXDAPWCSTKHHSXPXLGAAQ 755
           L  LAKVI+D F I EGLM             DGPS K W      S  +      GAA+
Sbjct: 157 LAPLAKVINDTFGIEEGLMTTVHATTATQKTVDGPSKKDWRGGRGASA-NIIPSSTGAAK 215

Query: 756 XCGXXYPLPPNGTLTGMPF 812
             G   P   NG LTGM F
Sbjct: 216 AVGKVIP-ALNGKLTGMAF 233


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,259,872
Number of Sequences: 5004
Number of extensions: 64460
Number of successful extensions: 197
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 412451140
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)