Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP16_F_F15
         (863 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    30   0.37 
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    30   0.49 
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz...    29   1.1  
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos...    28   2.0  
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch...    28   2.0  
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb...    27   2.6  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    27   4.5  
SPAC222.15 |meu13|SPAC821.01|Tat binding protein 1|Schizosacchar...    26   6.0  
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation...    26   6.0  
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p...    26   7.9  

>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 412

 Score = 30.3 bits (65), Expect = 0.37
 Identities = 27/113 (23%), Positives = 47/113 (41%)
 Frame = +1

Query: 82  PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 261
           P S S   + A    +      L +   V R    F + +EH+     K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229

Query: 262 SKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIER 420
           SKDA     A      S+     +   S + +L   N K++ +L+    ++ R
Sbjct: 230 SKDASSNLTACNRSGSSLSSNFYSSRLSKKTSLASLN-KSRASLQHKIMSLSR 281


>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 168

 Score = 29.9 bits (64), Expect = 0.49
 Identities = 13/46 (28%), Positives = 25/46 (54%)
 Frame = +1

Query: 295 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 432
           KDG+++   +LN FAK L  +    + +A +   +    IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156


>SPBC646.09c |int6|yin6|translation initiation factor
           eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 501

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
 Frame = +1

Query: 274 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 429
           +D + A+ +   SVLQ+L+   + +QG LG   N     AL Q + QN++   E
Sbjct: 79  EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132


>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
           Plc1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 899

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
 Frame = +1

Query: 169 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQQLNAFA 339
           ++   DFFK +    ++ H TL ++ NSL+    +K        +  G   +  +LN   
Sbjct: 56  KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115

Query: 340 KSLQGALGDA 369
           + LQG +  A
Sbjct: 116 R-LQGRMNSA 124


>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 803

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
 Frame = +1

Query: 229 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 390
           T++ +  SL K  D     +  ++  ES L  L      L  A      L D+  KAK  
Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411

Query: 391 LEQSRQNIERTAEE 432
           LEQ+R  +ERT  E
Sbjct: 412 LEQARIELERTQRE 425


>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 631

 Score = 27.5 bits (58), Expect = 2.6
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = -2

Query: 406 ASTVPKPPWPCRSRLRALPG 347
           AST+ K PWP  + L  +PG
Sbjct: 38  ASTLEKEPWPASTALLVMPG 57


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1485

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
 Frame = +1

Query: 175 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 294
           D   +F D++ H K FH   E+  +   +  +K   D  K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706


>SPAC222.15 |meu13|SPAC821.01|Tat binding protein
           1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 216

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 16/56 (28%), Positives = 28/56 (50%)
 Frame = +2

Query: 449 LNVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNEKLAPKIKXAYDDFAK 616
           LN   +   +REK+Q+  +   + S KL    +  V++ +++   K    Y DFAK
Sbjct: 120 LNNSLSPAEIREKIQSIDKEIEETSSKLESLRNGTVKQISKEAMQKTDKNY-DFAK 174


>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
           factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 321

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = +1

Query: 211 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 369
           +K+   + E+Q   +T      DFS   K   +     L+AF K L+  + GDA
Sbjct: 89  SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142


>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 394

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
 Frame = +1

Query: 139 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 315
           C  + +G  +RR  P +++ I      ++ KTL + FN+  + +++  + KA ++G   V
Sbjct: 13  CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72

Query: 316 LQQL 327
             Q+
Sbjct: 73  NNQI 76


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,470,426
Number of Sequences: 5004
Number of extensions: 40398
Number of successful extensions: 158
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 430470850
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)