Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP16_F_F10
         (890 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ...   102   8e-23
SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd...    87   4e-18

>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 937

 Score =  102 bits (244), Expect = 8e-23
 Identities = 65/199 (32%), Positives = 92/199 (46%)
 Frame = +2

Query: 89  MAQIISGIEVAGSIXNDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYXXXXXXXXXX 268
           MA ++ G  +A  +  +LR+Q++ ++S    F   L I+QVGGREDSNVY          
Sbjct: 1   MALLLEGTSLARKVREELREQISSIKSVDPYFNVSLKIIQVGGREDSNVYVRMKTRAANE 60

Query: 269 XXXXXXHIRLPRDITEIELLXKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDK 448
                 H+  P DITE +LL  I   NE P+VHGIIVQ+PL +   I+   +T+AV+P+K
Sbjct: 61  AGISCEHVNFPEDITEYDLLLAIKGFNEDPTVHGIIVQLPLPAH--INEQIITEAVAPEK 118

Query: 449 XVDXLNTINEGXXAVXDLSXFIPLXLQPGAWNSLRXPXSLYLXKMW*CSGGVPLWVXRCQ 628
            VD     N G     +           G    L+        K     G   +      
Sbjct: 119 DVDGFCETNLGKLTKREGQPLFTACTPKGIMCILKHYGINVQGKHAVVIGRSNIVGRPMS 178

Query: 629 SCXNXNTPTVTVCHSKTKN 685
                   TVT+CHSKT++
Sbjct: 179 ILLEKANATVTLCHSKTES 197



 Score = 35.1 bits (77), Expect = 0.014
 Identities = 19/55 (34%), Positives = 31/55 (56%), Gaps = 3/55 (5%)
 Frame = +1

Query: 508 IYSLXTPAGCVELIKXTXXTIPXKNVVVLRRSPIVGTPVSELLKXXH---XYCHS 663
           +++  TP G + ++K     +  K+ VV+ RS IVG P+S LL+  +     CHS
Sbjct: 139 LFTACTPKGIMCILKHYGINVQGKHAVVIGRSNIVGRPMSILLEKANATVTLCHS 193


>SPBC2G2.08 |ade9||C-1-
           tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr
           ogenase/methylenetetrahydrofolatecyclohydrolase/formylte
           trahydrofolatesynthetase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 969

 Score = 86.6 bits (205), Expect = 4e-18
 Identities = 59/198 (29%), Positives = 88/198 (44%)
 Frame = +2

Query: 92  AQIISGIEVAGSIXNDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYXXXXXXXXXXX 271
           A I++G  +A  +   + ++V  L++K   F+P LAIVQVG REDSNVY           
Sbjct: 33  ANILNGKLLARKLQRSVAEEVQALKAKDRNFKPALAIVQVGKREDSNVYVRMKEKAARLV 92

Query: 272 XXXXXHIRLPRDITEIELLXKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKX 451
                +   P  I    LL ++  LN+  +VHG++VQ+PL     ++   VT++++P K 
Sbjct: 93  GIDFKYCPFPETIQMPALLHELKKLNDDHTVHGVLVQLPLPKH--LNERTVTESITPPKD 150

Query: 452 VDXLNTINEGXXAVXDLSXFIPLXLQPGAWNSLRXPXSLYLXKMW*CSGGVPLWVXRCQS 631
           VD     N G  A  D +         G    L+              G   +       
Sbjct: 151 VDGFGAFNIGLLAKNDATPIHYPCTPKGIMELLKDNKISVAGLNAVVLGRSDIVGNPISY 210

Query: 632 CXNXNTPTVTVCHSKTKN 685
               +  TVTVCHSKTK+
Sbjct: 211 LLRKDNATVTVCHSKTKD 228



 Score = 41.5 bits (93), Expect = 2e-04
 Identities = 22/50 (44%), Positives = 29/50 (58%), Gaps = 3/50 (6%)
 Frame = +1

Query: 523 TPAGCVELIKXTXXTIPXKNVVVLRRSPIVGTPVSELLKXXH---XYCHS 663
           TP G +EL+K    ++   N VVL RS IVG P+S LL+  +     CHS
Sbjct: 175 TPKGIMELLKDNKISVAGLNAVVLGRSDIVGNPISYLLRKDNATVTVCHS 224


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,586,893
Number of Sequences: 5004
Number of extensions: 39509
Number of successful extensions: 77
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 64
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 75
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)