BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP16_F_C01
(926 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 34 0.025
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.057
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.075
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 32 0.13
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 0.16
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 31 0.23
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 27 0.74
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 29 1.2
SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 28 2.1
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.8
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 26 6.6
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 26 6.6
SPBC800.09 |sum2||G2/M transition checkpoint protein Sum2|Schizo... 26 6.6
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 34.3 bits (75), Expect = 0.025
Identities = 16/48 (33%), Positives = 17/48 (35%)
Frame = +2
Query: 347 PPSXPXXXXXHRGGXGXXPXXXRXPPPPPXPPPXXPAPPXXXPPAXPA 490
PP P + P PPPP PP PP PP PA
Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPA 1737
Score = 31.5 bits (68), Expect = 0.17
Identities = 14/28 (50%), Positives = 14/28 (50%), Gaps = 3/28 (10%)
Frame = +2
Query: 419 PPPPPX---PPPXXPAPPXXXPPAXPAP 493
PPPPP PPP P P P A P P
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 29.5 bits (63), Expect = 0.70
Identities = 13/32 (40%), Positives = 15/32 (46%), Gaps = 2/32 (6%)
Frame = +1
Query: 421 PPPXXAPPPXARP--PXXXPTRPASPXPXXRA 510
PPP PPP + P P P+ P P P A
Sbjct: 1709 PPPMSVPPPPSAPPMPAGPPSAPPPPLPASSA 1740
Score = 27.5 bits (58), Expect = 2.8
Identities = 12/36 (33%), Positives = 13/36 (36%)
Frame = +2
Query: 401 PXXXRXPPPPPXPPPXXPAPPXXXPPAXPAPXXXXG 508
P + PP P P PP PP AP G
Sbjct: 1691 PVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAG 1726
Score = 27.1 bits (57), Expect = 3.8
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +2
Query: 425 PPPXPPPXXPAPPXXXPPAXPAP 493
P P PPP PP PP P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGP 1727
Score = 26.6 bits (56), Expect = 5.0
Identities = 14/36 (38%), Positives = 17/36 (47%), Gaps = 6/36 (16%)
Frame = +1
Query: 421 PPPXXAPPPXARPPXXXP------TRPASPXPXXRA 510
PPP APP A PP P + P+ P P R+
Sbjct: 1715 PPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 33.1 bits (72), Expect = 0.057
Identities = 18/58 (31%), Positives = 20/58 (34%), Gaps = 2/58 (3%)
Frame = +2
Query: 326 PGXXGXGPPSXPXXXXXHRGGXGXXPXXXRXPP--PPPXPPPXXPAPPXXXPPAXPAP 493
P G P+ P R P PP PP PP P+ P P A P P
Sbjct: 393 PAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLP 450
Score = 30.3 bits (65), Expect = 0.40
Identities = 14/38 (36%), Positives = 14/38 (36%)
Frame = +2
Query: 347 PPSXPXXXXXHRGGXGXXPXXXRXPPPPPXPPPXXPAP 460
PPS P G P P PPP P P AP
Sbjct: 450 PPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAP 487
Score = 29.1 bits (62), Expect = 0.93
Identities = 17/56 (30%), Positives = 17/56 (30%)
Frame = +2
Query: 326 PGXXGXGPPSXPXXXXXHRGGXGXXPXXXRXPPPPPXPPPXXPAPPXXXPPAXPAP 493
P PPS P P P P PP PA P P PAP
Sbjct: 429 PSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAP 484
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/24 (50%), Positives = 12/24 (50%)
Frame = +2
Query: 422 PPPPXPPPXXPAPPXXXPPAXPAP 493
P P PP PAPP PA AP
Sbjct: 464 PAAPPLPPAAPAPPPAPAPAPAAP 487
Score = 27.5 bits (58), Expect = 2.8
Identities = 17/56 (30%), Positives = 18/56 (32%), Gaps = 9/56 (16%)
Frame = +2
Query: 347 PPSXPXXXXXHRGGXGXXPXXXRXPPPPP---------XPPPXXPAPPXXXPPAXP 487
PP P G P PPPPP PPP + PPA P
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPP 392
Score = 26.2 bits (55), Expect = 6.6
Identities = 19/61 (31%), Positives = 22/61 (36%), Gaps = 13/61 (21%)
Frame = +2
Query: 347 PPSXPXXXXXHRG----GXGXXPXXXRXPPPPPX---------PPPXXPAPPXXXPPAXP 487
PP P +RG G G PPPPP PP APP P + P
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAP 370
Query: 488 A 490
+
Sbjct: 371 S 371
Score = 26.2 bits (55), Expect = 6.6
Identities = 21/75 (28%), Positives = 22/75 (29%), Gaps = 4/75 (5%)
Frame = +1
Query: 286 AXPXXGAXPPQXXPRXXXXXXXQXSXEXSXXPGGXGXXPXGXXXXPP----PXXAPPPXA 453
A P G P S S P P G PP APP A
Sbjct: 402 ALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPA 461
Query: 454 RPPXXXPTRPASPXP 498
P P PA+P P
Sbjct: 462 GMPAAPPLPPAAPAP 476
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 32.7 bits (71), Expect = 0.075
Identities = 13/24 (54%), Positives = 13/24 (54%)
Frame = +2
Query: 419 PPPPPXPPPXXPAPPXXXPPAXPA 490
PPPPP PP A P PP PA
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPA 784
Score = 30.7 bits (66), Expect = 0.30
Identities = 14/27 (51%), Positives = 14/27 (51%), Gaps = 4/27 (14%)
Frame = +2
Query: 422 PPPPXPPPXX----PAPPXXXPPAXPA 490
PPPP PPP P PP PPA A
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPAVSA 787
Score = 29.1 bits (62), Expect = 0.93
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +2
Query: 425 PPPXPPPXXPAPPXXXPPAXPAPXXXXG 508
PPP PPP A PP P P G
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPAVSAG 788
Score = 26.6 bits (56), Expect = 5.0
Identities = 13/31 (41%), Positives = 13/31 (41%), Gaps = 1/31 (3%)
Frame = +2
Query: 419 PPPPPXP-PPXXPAPPXXXPPAXPAPXXXXG 508
P P P P P PAP PP P P G
Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAG 772
Score = 26.6 bits (56), Expect(2) = 0.98
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = +2
Query: 419 PPPPPXPPPXXPA 457
PPPPP PPP A
Sbjct: 775 PPPPPPPPPAVSA 787
Score = 26.2 bits (55), Expect = 6.6
Identities = 13/28 (46%), Positives = 14/28 (50%), Gaps = 1/28 (3%)
Frame = +2
Query: 413 RXPPPPPXPPPXXPAP-PXXXPPAXPAP 493
+ PPPPP P P P P P PAP
Sbjct: 730 KSPPPPP-PAVIVPTPAPAPIPVPPPAP 756
Score = 26.2 bits (55), Expect = 6.6
Identities = 15/38 (39%), Positives = 15/38 (39%), Gaps = 7/38 (18%)
Frame = +2
Query: 401 PXXXRXPPPPPXP----PPXXPAPP---XXXPPAXPAP 493
P P PPP P PP P PP PP P P
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 20.6 bits (41), Expect(2) = 0.98
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = +2
Query: 326 PGXXGXGPPSXP 361
PG G GPP P
Sbjct: 768 PGVAGAGPPPPP 779
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 31.9 bits (69), Expect = 0.13
Identities = 12/27 (44%), Positives = 13/27 (48%)
Frame = +2
Query: 428 PPXPPPXXPAPPXXXPPAXPAPXXXXG 508
PP PP P PP PP+ P P G
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPPG 31
Score = 30.7 bits (66), Expect = 0.30
Identities = 11/22 (50%), Positives = 11/22 (50%)
Frame = +2
Query: 422 PPPPXPPPXXPAPPXXXPPAXP 487
PPPP PPP P PP P
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30
Score = 27.1 bits (57), Expect = 3.8
Identities = 10/25 (40%), Positives = 12/25 (48%)
Frame = +1
Query: 424 PPXXAPPPXARPPXXXPTRPASPXP 498
PP PPP P P++P P P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPP 29
Score = 26.2 bits (55), Expect = 6.6
Identities = 11/22 (50%), Positives = 11/22 (50%), Gaps = 1/22 (4%)
Frame = +2
Query: 401 PXXXRXPPPPP-XPPPXXPAPP 463
P PPPPP PP P PP
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPP 27
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.2 bits (55), Expect(2) = 1.3
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +2
Query: 419 PPPPPXPPP 445
PPPPP PPP
Sbjct: 189 PPPPPPPPP 197
Score = 25.4 bits (53), Expect(2) = 0.16
Identities = 11/25 (44%), Positives = 11/25 (44%)
Frame = +2
Query: 419 PPPPPXPPPXXPAPPXXXPPAXPAP 493
P PPP PPP P P P P
Sbjct: 236 PAPPPPPPPTLP-PQSTNTSQLPMP 259
Score = 24.6 bits (51), Expect(2) = 0.16
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = +2
Query: 401 PXXXRXPPPPPXPP 442
P PPPPP PP
Sbjct: 184 PSDYNPPPPPPPPP 197
Score = 24.2 bits (50), Expect(2) = 1.3
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = +2
Query: 422 PPPPXPPPXXPAPP 463
PPPP P P PP
Sbjct: 273 PPPPATPSQPPRPP 286
Score = 22.6 bits (46), Expect(2) = 1.3
Identities = 8/15 (53%), Positives = 8/15 (53%)
Frame = +2
Query: 401 PXXXRXPPPPPXPPP 445
P PPPPP PP
Sbjct: 234 PLPAPPPPPPPTLPP 248
Score = 20.6 bits (41), Expect(2) = 1.3
Identities = 7/15 (46%), Positives = 7/15 (46%)
Frame = +2
Query: 431 PXPPPXXPAPPXXXP 475
P P P P PP P
Sbjct: 234 PLPAPPPPPPPTLPP 248
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 31.1 bits (67), Expect = 0.23
Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 1/31 (3%)
Frame = +2
Query: 401 PXXXRXPP-PPPXPPPXXPAPPXXXPPAXPA 490
P + PP P PPP PA P PP+ P+
Sbjct: 159 PIPSKAPPIPSSLPPPAQPAAPVKSPPSAPS 189
Score = 27.5 bits (58), Expect = 2.8
Identities = 14/29 (48%), Positives = 16/29 (55%), Gaps = 3/29 (10%)
Frame = +1
Query: 421 PPPXXAPP-PXA--RPPXXXPTRPASPXP 498
PP APP P + RPP P RP+ P P
Sbjct: 124 PPSAPAPPTPQSELRPPTSAPPRPSIPPP 152
Score = 27.1 bits (57), Expect = 3.8
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +2
Query: 419 PPPPPXPPPXXPAPPXXXPP 478
P PPP PP PA PP
Sbjct: 3 PAPPPPPPAPAPAAAAPAPP 22
Score = 27.1 bits (57), Expect = 3.8
Identities = 14/35 (40%), Positives = 14/35 (40%), Gaps = 5/35 (14%)
Frame = +2
Query: 401 PXXXRXPPP-----PPXPPPXXPAPPXXXPPAXPA 490
P PPP PP P P P PPA PA
Sbjct: 144 PPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPA 178
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 26.6 bits (56), Expect(2) = 0.74
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = +2
Query: 419 PPPPPXPPPXXPA 457
PPPPP PPP A
Sbjct: 945 PPPPPPPPPLVSA 957
Score = 21.0 bits (42), Expect(2) = 0.74
Identities = 7/14 (50%), Positives = 7/14 (50%)
Frame = +2
Query: 401 PXXXRXPPPPPXPP 442
P P PPP PP
Sbjct: 899 PTIITHPTPPPPPP 912
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 28.7 bits (61), Expect = 1.2
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = -3
Query: 486 GXAGGXXXGGAGXXGGGXGGGGGXRXXXG 400
G GG G G GGG GG G R G
Sbjct: 23 GGRGGFGGGRGGARGGGRGGARGGRGGRG 51
Score = 26.6 bits (56), Expect = 5.0
Identities = 13/27 (48%), Positives = 13/27 (48%)
Frame = -3
Query: 492 GAGXAGGXXXGGAGXXGGGXGGGGGXR 412
G G A G GGA GG GG G R
Sbjct: 31 GRGGARGGGRGGARGGRGGRGGARGGR 57
Score = 26.2 bits (55), Expect = 6.6
Identities = 14/32 (43%), Positives = 14/32 (43%), Gaps = 1/32 (3%)
Frame = -3
Query: 492 GAGXAGGXXXGGAGXXGGGXGGG-GGXRXXXG 400
G G G G G GG GGG GG R G
Sbjct: 17 GRGGFNGGRGGFGGGRGGARGGGRGGARGGRG 48
Score = 25.8 bits (54), Expect = 8.7
Identities = 13/27 (48%), Positives = 14/27 (51%), Gaps = 2/27 (7%)
Frame = -3
Query: 492 GAGXAGGXXXGGAGXXGGGXGG--GGG 418
G+ G GG G GGG GG GGG
Sbjct: 13 GSRGGRGGFNGGRGGFGGGRGGARGGG 39
>SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1372
Score = 27.9 bits (59), Expect = 2.1
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = +2
Query: 413 RXPPPPPXPPPXXPAP 460
+ PPP P PPP P P
Sbjct: 804 KAPPPAPLPPPAPPLP 819
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.5 bits (58), Expect = 2.8
Identities = 16/35 (45%), Positives = 16/35 (45%), Gaps = 4/35 (11%)
Frame = +1
Query: 406 GXXXXPPPXXAPP---PXAR-PPXXXPTRPASPXP 498
G PPP APP P A PP PT A P P
Sbjct: 1206 GVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVP 1240
Score = 27.1 bits (57), Expect = 3.8
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +2
Query: 419 PPPPPXPPPXXPAPPXXXPPAXP 487
P PPP P P P PP P
Sbjct: 1190 PVPPPSEAPPVPKPSVGVPPVPP 1212
Score = 26.2 bits (55), Expect = 6.6
Identities = 11/25 (44%), Positives = 11/25 (44%)
Frame = +2
Query: 419 PPPPPXPPPXXPAPPXXXPPAXPAP 493
P PPP P P P PP P P
Sbjct: 1209 PVPPPSTAPPVPTPSAGLPPV-PVP 1232
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 26.2 bits (55), Expect = 6.6
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +2
Query: 419 PPPPPXPPP 445
PPPPP PPP
Sbjct: 306 PPPPPPPPP 314
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 26.2 bits (55), Expect = 6.6
Identities = 12/28 (42%), Positives = 12/28 (42%), Gaps = 1/28 (3%)
Frame = +2
Query: 413 RXPPPPPXPPPXXPAP-PXXXPPAXPAP 493
R PPPPP P P P P P P
Sbjct: 355 RPPPPPPMPAPIYNVPNVPTVPTVSPNP 382
>SPBC800.09 |sum2||G2/M transition checkpoint protein
Sum2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 26.2 bits (55), Expect = 6.6
Identities = 16/53 (30%), Positives = 18/53 (33%)
Frame = +2
Query: 323 TPGXXGXGPPSXPXXXXXHRGGXGXXPXXXRXPPPPPXPPPXXPAPPXXXPPA 481
TP PP+ P + G PP P P P AP PPA
Sbjct: 82 TPSAPPVQPPNDPAIIGSNSGQYNWNQAQTAQPPQPVQPNPYG-APYQQAPPA 133
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 835,826
Number of Sequences: 5004
Number of extensions: 10890
Number of successful extensions: 323
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 50
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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