Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP16_F_A04
(776 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 33 0.060
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.24
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.74
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 3.0
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 23 6.2
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 25 9.2
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 32.7 bits (71), Expect = 0.060
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +1
Query: 661 TPPPPXPXXPPPQXXXPXXXKXXXPPPPP 747
TPPPP PPP P PPPP
Sbjct: 1706 TPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 30.3 bits (65), Expect = 0.32
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = +3
Query: 663 PPPPXPXXPPPXXXXXXXXXKXXPPPPPXP 752
PPPP PPP PPPP P
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 30.7 bits (66), Expect = 0.24
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +1
Query: 664 PPPPXPXXPPPQXXXPXXXKXXXPPPPP 747
PP P PPP P PPPPP
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 30.3 bits (65), Expect = 0.32
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = +3
Query: 663 PPPPXPXXPPPXXXXXXXXXKXXPPPPPXP 752
PP P PPP PPPPP P
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 27.9 bits (59), Expect = 1.7
Identities = 13/32 (40%), Positives = 13/32 (40%), Gaps = 3/32 (9%)
Frame = +3
Query: 666 PPPXPXX---PPPXXXXXXXXXKXXPPPPPXP 752
PPP P PPP PPPPP P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 27.1 bits (57), Expect = 3.0
Identities = 12/28 (42%), Positives = 12/28 (42%), Gaps = 1/28 (3%)
Frame = +1
Query: 667 PPPXPXXP-PPQXXXPXXXKXXXPPPPP 747
PPP P PP P PPPPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPP 779
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.1 bits (62), Expect = 0.74
Identities = 19/65 (29%), Positives = 20/65 (30%)
Frame = +1
Query: 553 PPPPPXXFFFXIYSPPAGGXGXGXKKXFFFSXGXXKTPPPPXPXXPPPQXXXPXXXKXXX 732
PPPPP PP G S PPPP P +
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGS---------SNSSLPPPPPPPRSNAAGSIPLPPQGRSAP 361
Query: 733 PPPPP 747
PPPPP
Sbjct: 362 PPPPP 366
Score = 25.8 bits (54), Expect = 6.9
Identities = 10/28 (35%), Positives = 11/28 (39%)
Frame = +3
Query: 663 PPPPXPXXPPPXXXXXXXXXKXXPPPPP 746
PPPP P + PPPPP
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPP 365
Score = 25.4 bits (53), Expect = 9.2
Identities = 18/71 (25%), Positives = 20/71 (28%)
Frame = +1
Query: 553 PPPPPXXFFFXIYSPPAGGXGXGXKKXFFFSXGXXKTPPPPXPXXPPPQXXXPXXXKXXX 732
PPP P + SPP + PP P P K
Sbjct: 253 PPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPP 312
Query: 733 PPPPPXXXXXG 765
PPPPP G
Sbjct: 313 PPPPPSRRNRG 323
Score = 25.4 bits (53), Expect = 9.2
Identities = 14/41 (34%), Positives = 14/41 (34%), Gaps = 5/41 (12%)
Frame = +1
Query: 658 KTPPPPXPXX-----PPPQXXXPXXXKXXXPPPPPXXXXXG 765
K PPPP P PP PPPPP G
Sbjct: 309 KRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAG 349
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.1 bits (57), Expect = 3.0
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +1
Query: 679 PXXPPPQXXXPXXXKXXXPPPPP 747
P PPP P PPPPP
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPP 28
Score = 26.2 bits (55), Expect = 5.2
Identities = 10/25 (40%), Positives = 10/25 (40%)
Frame = +3
Query: 678 PXXPPPXXXXXXXXXKXXPPPPPXP 752
P PPP PPPPP P
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 23.4 bits (48), Expect(2) = 6.2
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +3
Query: 660 NPPPPXPXXPPP 695
NPPPP P PPP
Sbjct: 188 NPPPPPP--PPP 197
Score = 20.6 bits (41), Expect(2) = 6.2
Identities = 8/23 (34%), Positives = 8/23 (34%)
Frame = +3
Query: 678 PXXPPPXXXXXXXXXKXXPPPPP 746
P PP PPPPP
Sbjct: 221 PEIPPTYTPKQADPLPAPPPPPP 243
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 25.4 bits (53), Expect = 9.2
Identities = 12/27 (44%), Positives = 12/27 (44%)
Frame = -1
Query: 746 GGGGGXXFXXXXGXXXXGGGXXGXGGG 666
GG GG G GGG G GGG
Sbjct: 224 GGFGGGPGGFEGGPGGFGGGPGGFGGG 250
Score = 25.4 bits (53), Expect = 9.2
Identities = 12/27 (44%), Positives = 12/27 (44%)
Frame = -1
Query: 746 GGGGGXXFXXXXGXXXXGGGXXGXGGG 666
GG GG G GGG G GGG
Sbjct: 238 GGFGGGPGGFGGGLGGFGGGPGGFGGG 264
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,192,152
Number of Sequences: 5004
Number of extensions: 36364
Number of successful extensions: 243
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 40
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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