Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP15_F_O13
(883 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 173 4e-44
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 173 4e-44
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 122 7e-29
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 74 2e-14
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 69 1e-12
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 57 4e-09
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 56 9e-09
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 36 0.006
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.031
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.031
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.031
SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schi... 31 0.29
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 30 0.50
SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24 famil... 29 0.66
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 28 2.0
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 27 3.5
SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 26 8.2
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 173 bits (420), Expect = 4e-44
Identities = 87/130 (66%), Positives = 104/130 (80%), Gaps = 1/130 (0%)
Frame = +3
Query: 120 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 299
MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR
Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60
Query: 300 KDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEV 476
DEQ+R +TIKSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEV
Sbjct: 61 ADEQERGVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEV 115
Query: 477 TAALRVTDGA 506
TAALRVTDGA
Sbjct: 116 TAALRVTDGA 125
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 173 bits (420), Expect = 4e-44
Identities = 87/130 (66%), Positives = 104/130 (80%), Gaps = 1/130 (0%)
Frame = +3
Query: 120 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 299
MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR
Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60
Query: 300 KDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEV 476
DEQ+R +TIKSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEV
Sbjct: 61 ADEQERGVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEV 115
Query: 477 TAALRVTDGA 506
TAALRVTDGA
Sbjct: 116 TAALRVTDGA 125
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 122 bits (294), Expect = 7e-29
Identities = 63/123 (51%), Positives = 89/123 (72%)
Frame = +3
Query: 138 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 317
+++ + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R
Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66
Query: 318 CITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVT 497
IT+KS+AIS+FF++ I+ D++ + EK +LINLIDSPGHVDFSSEV++A R+
Sbjct: 67 GITMKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLC 118
Query: 498 DGA 506
DGA
Sbjct: 119 DGA 121
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 74.1 bits (174), Expect = 2e-14
Identities = 49/134 (36%), Positives = 71/134 (52%), Gaps = 7/134 (5%)
Frame = +3
Query: 126 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA--GETRF-- 287
N + E DKKR IRN+ + AH+D GK+T T+ ++ G I G+
Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGA 100
Query: 288 -TDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDF 464
D + E+++ ITI+S A +E + N Q+ EK + IN+ID+PGH+DF
Sbjct: 101 KMDFMELEREKGITIQSAATHCTWERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDF 158
Query: 465 SSEVTAALRVTDGA 506
+ EV ALRV DGA
Sbjct: 159 TIEVERALRVLDGA 172
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 68.5 bits (160), Expect = 1e-12
Identities = 42/120 (35%), Positives = 65/120 (54%)
Frame = +3
Query: 144 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCI 323
+RG+ + +RN +VIAH+DHGKSTL+D ++ G+I +F D + E+ R I
Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLDKLEVERRRGI 106
Query: 324 TIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDG 503
T+K+ SM + + +L+NLID+PGHVDF +EV +L +G
Sbjct: 107 TVKAQTCSMIYYYH----------------GQSYLLNLIDTPGHVDFRAEVMHSLAACEG 150
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 56.8 bits (131), Expect = 4e-09
Identities = 44/115 (38%), Positives = 59/115 (51%), Gaps = 3/115 (2%)
Frame = +3
Query: 171 NIRNMSVIAHVDHGKSTLTDSLVSKAGI---IAGARAGETRFTDTRKDEQDRCITIKSTA 341
+IRN+ +IAH+D GK+TLT+ ++ G G+T D E+ R ITI S A
Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDT-VMDYLPAERQRGITINSAA 85
Query: 342 ISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 506
IS T +QR INLID+PGH DF+ EV ++ V DGA
Sbjct: 86 IS------------FTWRNQR--------INLIDTPGHADFTFEVERSVAVLDGA 120
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 55.6 bits (128), Expect = 9e-09
Identities = 38/119 (31%), Positives = 59/119 (49%), Gaps = 1/119 (0%)
Frame = +3
Query: 150 GMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGI-IAGARAGETRFTDTRKDEQDRCIT 326
G++ ++R+ V H+ HGKS L D LV + R+TDT E++R ++
Sbjct: 132 GLLTGTDDVRSFIVAGHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMS 191
Query: 327 IKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDG 503
IKST +++ D + K+ F ID+PGHVDF EV A + ++DG
Sbjct: 192 IKSTPLTLAVS------------DMKGKT---FAFQCIDTPGHVDFVDEVAAPMAISDG 235
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 36.3 bits (80), Expect = 0.006
Identities = 21/61 (34%), Positives = 32/61 (52%)
Frame = +3
Query: 162 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTA 341
KK ++ N+ I HVDHGK+TLT ++ + A + D +E+ R ITI S
Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITISSAH 108
Query: 342 I 344
+
Sbjct: 109 V 109
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 33.9 bits (74), Expect = 0.031
Identities = 16/32 (50%), Positives = 20/32 (62%)
Frame = +3
Query: 156 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 251
M K++ N+ VI HVD GKST T L+ K G
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 33.9 bits (74), Expect = 0.031
Identities = 16/32 (50%), Positives = 20/32 (62%)
Frame = +3
Query: 156 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 251
M K++ N+ VI HVD GKST T L+ K G
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 33.9 bits (74), Expect = 0.031
Identities = 16/32 (50%), Positives = 20/32 (62%)
Frame = +3
Query: 156 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 251
M K++ N+ VI HVD GKST T L+ K G
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32
>SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit
Mcm5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 30.7 bits (66), Expect = 0.29
Identities = 28/117 (23%), Positives = 52/117 (44%), Gaps = 1/117 (0%)
Frame = +3
Query: 135 VDEIRGMMDKKR-NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQ 311
+DE M D+ R I + K+ +T L S+ ++A A R+ D + +
Sbjct: 435 IDEFDKMRDEDRVAIHEAMEQQTISIAKAGITTILNSRTSVLAAANPIFGRYDDMKTPGE 494
Query: 312 DRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTA 482
+ I +ST +S F D++FI + E ++ ++I+ ++ SSE A
Sbjct: 495 N--IDFQSTILSRF------DMIFIVKDEHDETKDRNIARHVINLHTNLQESSETLA 543
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 29.9 bits (64), Expect = 0.50
Identities = 23/67 (34%), Positives = 34/67 (50%), Gaps = 4/67 (5%)
Frame = +3
Query: 183 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR----FTDTRKDEQDRCITIKSTAISM 350
++++ HVDHGK+TL D+ K+ I + G T+ FT D+ + IT T M
Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQKIGAFT-VPFDKGSKFITFLDTPGHM 231
Query: 351 FFELEEK 371
FE K
Sbjct: 232 AFEAMRK 238
>SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 195
Score = 29.5 bits (63), Expect = 0.66
Identities = 16/54 (29%), Positives = 27/54 (50%)
Frame = -3
Query: 176 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQLPYASNGVRFEKGXNLKEFPIVS 15
DIPL + P D + RE + RW + +L + Y ++ F G + + F +S
Sbjct: 102 DIPLRLIQPYDPLSRETVYMRWKELAMLDKTVDYQNHNQSFPFGISYEGFYYIS 155
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.9 bits (59), Expect = 2.0
Identities = 26/92 (28%), Positives = 39/92 (42%)
Frame = +3
Query: 180 NMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAISMFFE 359
N+ I HVD GKSTL +++ G++ R E + E + S A+ E
Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV-DKRTMEK--IEREAKEAGKESWYLSWALDSTSE 296
Query: 360 LEEKDLVFITNPDQREKSEKGFLINLIDSPGH 455
EK E + F +L+D+PGH
Sbjct: 297 EREKGKTVEVGRAYFETEHRRF--SLLDAPGH 326
>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
2|||Manual
Length = 207
Score = 27.1 bits (57), Expect = 3.5
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = -1
Query: 469 EEKSTCPGESIKLIKKPFSLFSRWSGFVMNTKSFSS-SSKNIEMAVDLMVMQRSCSSLRV 293
EE + P + K KK S F+ T +S S++NI+ A+DL+ + S S ++
Sbjct: 66 EEMESLPSKGGKGSKKAAKKNSSLDAFLNETPQTASYSARNIDDALDLLSLNNSSSKDKI 125
>SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting
endonuclease Cce1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 258
Score = 25.8 bits (54), Expect = 8.2
Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = -1
Query: 421 PFSLFSRWSGFVMNTK-SFSSSSKNIEMAVDLMVMQR 314
P S +S W+ V+NTK SFS ++M +L+ Q+
Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELIDGQK 203
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,012,881
Number of Sequences: 5004
Number of extensions: 56517
Number of successful extensions: 172
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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