BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP15_F_N24
(1246 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.19
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.33
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.77
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 29 1.0
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 1.8
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 2.8
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 28 3.1
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 31.9 bits (69), Expect = 0.19
Identities = 16/40 (40%), Positives = 16/40 (40%), Gaps = 4/40 (10%)
Frame = +2
Query: 1136 LXXPLPTXP----PTPTXXPXXTTLXPPXXXSPPPPPXPP 1243
L P P P PTP P P PPPPP PP
Sbjct: 729 LKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768
Score = 31.9 bits (69), Expect = 0.19
Identities = 14/35 (40%), Positives = 15/35 (42%), Gaps = 2/35 (5%)
Frame = +2
Query: 1145 PLPTXPPTPTXXPXXTTLXPP--XXXSPPPPPXPP 1243
P+P PP P PP PPPPP PP
Sbjct: 748 PIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 26.6 bits (56), Expect = 7.2
Identities = 12/30 (40%), Positives = 13/30 (43%)
Frame = +2
Query: 1145 PLPTXPPTPTXXPXXTTLXPPXXXSPPPPP 1234
P+ PP P P PP PPPPP
Sbjct: 756 PIMGGPPPPPPPPGVAGAGPPPP--PPPPP 783
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.1 bits (67), Expect = 0.33
Identities = 13/31 (41%), Positives = 14/31 (45%)
Frame = +2
Query: 1148 LPTXPPTPTXXPXXTTLXPPXXXSPPPPPXP 1240
LP PP P + PP S PPPP P
Sbjct: 336 LPPPPPPPRSNAAGSIPLPPQGRSAPPPPPP 366
Score = 29.9 bits (64), Expect = 0.77
Identities = 13/30 (43%), Positives = 14/30 (46%)
Frame = +2
Query: 1151 PTXPPTPTXXPXXTTLXPPXXXSPPPPPXP 1240
P PP P P L PP +PPP P P
Sbjct: 454 PIAPPLPAGMPAAPPL-PPAAPAPPPAPAP 482
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.9 bits (64), Expect = 0.77
Identities = 13/31 (41%), Positives = 14/31 (45%)
Frame = +2
Query: 1151 PTXPPTPTXXPXXTTLXPPXXXSPPPPPXPP 1243
PT PP P P + PP PP P PP
Sbjct: 1705 PTPPPPPMSVPPPPSA-PPMPAGPPSAPPPP 1734
Score = 28.7 bits (61), Expect = 1.8
Identities = 12/32 (37%), Positives = 12/32 (37%)
Frame = +2
Query: 1145 PLPTXPPTPTXXPXXTTLXPPXXXSPPPPPXP 1240
P P PP P P S PPPP P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 29.5 bits (63), Expect = 1.0
Identities = 17/42 (40%), Positives = 17/42 (40%)
Frame = -1
Query: 673 GGGGGGXXGGGXRLDXHXXXGGGGXRGXTXGIKXGV*GEXXG 548
GGG GG GGG G GG RG G G G G
Sbjct: 29 GGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGGRGGAKGG 70
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 309
Score = 28.7 bits (61), Expect = 1.8
Identities = 13/33 (39%), Positives = 16/33 (48%), Gaps = 1/33 (3%)
Frame = +2
Query: 1145 PLPTXPP-TPTXXPXXTTLXPPXXXSPPPPPXP 1240
P P+ PP +P P + PP S PPP P
Sbjct: 145 PRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQP 177
Score = 27.1 bits (57), Expect = 5.4
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = +2
Query: 1145 PLPTXPPTPTXXPXXTTLXPPXXXSPPPPP 1234
P PPTP T PP PPP P
Sbjct: 125 PSAPAPPTPQSELRPPTSAPPRPSIPPPSP 154
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 26.6 bits (56), Expect = 7.2
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +2
Query: 1151 PTXPPTPTXXPXXTTLXPPXXXSPPPPP 1234
P PP P P PP PPPPP
Sbjct: 6 PGNPPPPPPPPG---FEPPSQPPPPPPP 30
Score = 23.8 bits (49), Expect(2) = 2.8
Identities = 7/9 (77%), Positives = 8/9 (88%)
Frame = +2
Query: 1217 SPPPPPXPP 1243
+PPPPP PP
Sbjct: 8 NPPPPPPPP 16
Score = 22.2 bits (45), Expect(2) = 2.8
Identities = 7/11 (63%), Positives = 7/11 (63%)
Frame = +2
Query: 1202 PPXXXSPPPPP 1234
PP PPPPP
Sbjct: 5 PPGNPPPPPPP 15
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.9 bits (59), Expect = 3.1
Identities = 13/33 (39%), Positives = 14/33 (42%)
Frame = +2
Query: 1145 PLPTXPPTPTXXPXXTTLXPPXXXSPPPPPXPP 1243
P P+ P P T P PPPPP PP
Sbjct: 167 PPPSFQPPSAAAP--ATSLPSDYNPPPPPPPPP 197
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,238,114
Number of Sequences: 5004
Number of extensions: 11227
Number of successful extensions: 135
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 36
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 96
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 677341880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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