Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP15_F_K22
         (900 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|...   130   3e-31
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase...   126   4e-30
SPBC17G9.06c |||N-acetyltransferase |Schizosaccharomyces pombe|c...    28   1.6  
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate...    27   2.7  
SPCC569.08c |ade5|ade8|phosphoribosylglycinamide formyltransfera...    27   3.6  
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy...    26   8.4  

>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
           Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score =  130 bits (313), Expect = 3e-31
 Identities = 82/166 (49%), Positives = 101/166 (60%), Gaps = 4/166 (2%)
 Frame = +3

Query: 315 LFVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 494
           L ++G+ I V +ERDP  I W  +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAPS 
Sbjct: 67  LVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAPSK 126

Query: 495 DAPMFVVGVNLXAYDPSFKVISNASWHHKLSCPTWQRL---FMITLKWLRA**LLFMPQ- 662
           DAPMFVVGVNL  ++PS KVISNAS       P  + +   F I    +         Q 
Sbjct: 127 DAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTATQK 186

Query: 663 LLHRKLLMDLLENYGVMAVVLNKKSFLASTGAAKAVGKVIPAPNGQ 800
            +      D     G  A ++      +STGAAKAVGKVIPA NG+
Sbjct: 187 TVDGPSKKDWRGGRGASANIIP-----SSTGAAKAVGKVIPALNGK 227



 Score =   99 bits (238), Expect = 4e-22
 Identities = 47/64 (73%), Positives = 54/64 (84%), Gaps = 1/64 (1%)
 Frame = +2

Query: 131 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 307
           K+GINGFGRIGR+VLR +I  G  QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + 
Sbjct: 5   KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64

Query: 308 GFLV 319
           G LV
Sbjct: 65  GKLV 68



 Score = 49.2 bits (112), Expect = 8e-07
 Identities = 27/62 (43%), Positives = 31/62 (50%)
 Frame = +2

Query: 587 LPHLAKVIHDNFEMVEGLMXXXXXXXXXXXXXDGPSGKLWRDGRGAQQKIIPGLYWCCQS 766
           L  LAKVI+D F + EGLM             DGPS K WR GRGA   IIP      ++
Sbjct: 157 LAPLAKVINDTFGIEEGLMTTVHATTATQKTVDGPSKKDWRGGRGASANIIPSSTGAAKA 216

Query: 767 CG 772
            G
Sbjct: 217 VG 218


>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
           Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 336

 Score =  126 bits (304), Expect = 4e-30
 Identities = 80/166 (48%), Positives = 100/166 (60%), Gaps = 4/166 (2%)
 Frame = +3

Query: 315 LFVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 494
           L ++GN I V +ERDP  I W  +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAPS 
Sbjct: 67  LVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAPSK 126

Query: 495 DAPMFVVGVNLXAYDPSFKVISNASWHHKLSCPTWQRL---FMITLKWLRA**LLFMPQ- 662
           DAPM+VVGVN   ++PS KVISNAS       P  + +   F I    +         Q 
Sbjct: 127 DAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTATQK 186

Query: 663 LLHRKLLMDLLENYGVMAVVLNKKSFLASTGAAKAVGKVIPAPNGQ 800
            +      D     G  A ++      +STGAAKAVGKVIPA NG+
Sbjct: 187 TVDGPSKKDWRGGRGASANIIP-----SSTGAAKAVGKVIPALNGK 227



 Score =  102 bits (245), Expect = 6e-23
 Identities = 48/64 (75%), Positives = 55/64 (85%), Gaps = 1/64 (1%)
 Frame = +2

Query: 131 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 307
           K+GINGFGRIGR+VLR A + K  QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D
Sbjct: 5   KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64

Query: 308 GFLV 319
           G LV
Sbjct: 65  GKLV 68



 Score = 49.2 bits (112), Expect = 8e-07
 Identities = 27/62 (43%), Positives = 31/62 (50%)
 Frame = +2

Query: 587 LPHLAKVIHDNFEMVEGLMXXXXXXXXXXXXXDGPSGKLWRDGRGAQQKIIPGLYWCCQS 766
           L  LAKVI+D F + EGLM             DGPS K WR GRGA   IIP      ++
Sbjct: 157 LAPLAKVINDTFGIEEGLMTTVHATTATQKTVDGPSKKDWRGGRGASANIIPSSTGAAKA 216

Query: 767 CG 772
            G
Sbjct: 217 VG 218


>SPBC17G9.06c |||N-acetyltransferase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 334

 Score = 28.3 bits (60), Expect = 1.6
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +1

Query: 688 TFWKIMA*WPWCSTKNHSWPLLVLPKLWVR 777
           +FW+I +  PW  T+   WPL  LP   +R
Sbjct: 109 SFWQIPS--PWMYTRCSDWPLSYLPNQVIR 136


>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
           transferase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 453

 Score = 27.5 bits (58), Expect = 2.7
 Identities = 15/45 (33%), Positives = 26/45 (57%)
 Frame = -1

Query: 189 SIEARSTKRPMRPNPLIPIFDMLSASYRKFVE*LTTTTYAQVQDG 55
           ++ A+ TK P+RP  + P   + SA + ++   +  T  AQVQ+G
Sbjct: 179 ALAAKLTK-PIRPLWVTPASRLTSAQFEEYFTVVLVTASAQVQNG 222


>SPCC569.08c |ade5|ade8|phosphoribosylglycinamide
           formyltransferase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 207

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 14/26 (53%), Positives = 18/26 (69%), Gaps = 1/26 (3%)
 Frame = -3

Query: 337 ILLPL-TNKESILNLNTALKTAMGGI 263
           +L+PL TNK  I+NL+ AL  A  GI
Sbjct: 107 VLIPLETNKIGIINLHPALPGAFNGI 132


>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1583

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
 Frame = -3

Query: 325 LTNKESILNLNTALKTAMGGIILEKINHIVK---TDERVIYSDHLSSLFNR 182
           L N+ESIL+L   L +     + E I++IV+   +D R     HLS L  +
Sbjct: 342 LQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQK 392


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,646,752
Number of Sequences: 5004
Number of extensions: 76627
Number of successful extensions: 237
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 235
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)