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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP15_F_J04
         (886 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    31   0.17 
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    30   0.38 
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    27   3.6  
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe...    27   3.6  
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    27   4.7  
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    27   4.7  
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||...    27   4.7  
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M...    26   6.2  

>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 513

 Score = 31.5 bits (68), Expect = 0.17
 Identities = 14/43 (32%), Positives = 17/43 (39%)
 Frame = +3

Query: 699 PLXRPPXSXPXRLPEYLSXLSPFXXTVGXSPXPPPXXXSPXXG 827
           PL +PP       PE+    SP   +    P PPP    P  G
Sbjct: 211 PLNQPPSYAASTQPEFQQTTSPIFSSSSTPPPPPPRPSQPTSG 253


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 30.3 bits (65), Expect = 0.38
 Identities = 24/77 (31%), Positives = 28/77 (36%), Gaps = 2/77 (2%)
 Frame = +3

Query: 651 NPDRTIXIPGVSPPXXPLXRPPXSXPXRLPEYLSXLSPFXXTVGXSPXPPPXXXSP--XX 824
           N  RT   P  +PP  P   PP S P   P  L   +P        P PP    +P    
Sbjct: 408 NASRTSTPPVPTPPSLPPSAPP-SLPPSAPPSLPMGAP-----AAPPLPPSAPIAPPLPA 461

Query: 825 GXXSXPPXPGLXXXKPP 875
           G  + PP P      PP
Sbjct: 462 GMPAAPPLPPAAPAPPP 478



 Score = 27.5 bits (58), Expect = 2.7
 Identities = 22/65 (33%), Positives = 22/65 (33%), Gaps = 1/65 (1%)
 Frame = +1

Query: 685 PPRXXPSCAPLXPXPXAYRNTCPXFLPSGKPWXXPXXP-PPXXFPXXRGXXPXPPXXGXX 861
           PP   PS  P  P P A     P  LP G P   P  P  P   P   G    PP     
Sbjct: 420 PPSLPPSAPPSLP-PSA-----PPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAA 473

Query: 862 XXNPP 876
              PP
Sbjct: 474 PAPPP 478



 Score = 26.2 bits (55), Expect = 6.2
 Identities = 16/61 (26%), Positives = 19/61 (31%)
 Frame = +1

Query: 703 SCAPLXPXPXAYRNTCPXFLPSGKPWXXPXXPPPXXFPXXRGXXPXPPXXGXXXXNPPFX 882
           S + L P P   R+     +P          PPP       G  P P        NPP  
Sbjct: 332 SNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAP 391

Query: 883 P 885
           P
Sbjct: 392 P 392


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 20/69 (28%), Positives = 23/69 (33%)
 Frame = +3

Query: 678  GVSPPXXPLXRPPXSXPXRLPEYLSXLSPFXXTVGXSPXPPPXXXSPXXGXXSXPPXPGL 857
            G   P  P+  PP       P  +S  +P       S  PPP       G  S PP P L
Sbjct: 1679 GGMAPAHPVSTPPVRPQSAAPPQMS--APTPPPPPMSVPPPPSAPPMPAGPPSAPPPP-L 1735

Query: 858  XXXKPPXXP 884
                 P  P
Sbjct: 1736 PASSAPSVP 1744


>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 273

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 22/74 (29%), Positives = 24/74 (32%)
 Frame = -3

Query: 875 GGFXXXQPXXGGXGXXPRXWGXXXGGGXXGXXHGXPEGRKXGQVFR*AXGXGXRGAXEGX 696
           GGF       GG    P  +G   G G  G  HG   G   G       G G  G   G 
Sbjct: 188 GGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGG-HGGFGGGPGGFEGGPGGFGGGPGG 246

Query: 695 XRGGNAWYXYSPVG 654
             GG   +   P G
Sbjct: 247 FGGGLGGFGGGPGG 260


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 20/58 (34%), Positives = 22/58 (37%), Gaps = 1/58 (1%)
 Frame = +3

Query: 675  PGVSPPXXPLXRPPXSXPXRLPEYLSXLSPFXXTVGXSPXPPPXXXS-PXXGXXSXPP 845
            P VS P      P  S   +LP   S L P   T    P PPP   + P     S PP
Sbjct: 1036 PTVSVPSPSALSP--SVTPQLPPVSSRLPPVSATRPQIPQPPPVSTALPSSSAVSRPP 1091


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 19/69 (27%), Positives = 25/69 (36%), Gaps = 1/69 (1%)
 Frame = +3

Query: 672  IPGVSPPXXPLXRPPXSXPXRLPEYLSXLSPFXXTVGXSPXPPPXXXSPXXGXXS-XPPX 848
            +P  S    P+ +P    P   P   +   P   +VG  P PPP    P     +  PP 
Sbjct: 1171 VPAPSSGIPPVPKPAAGVPPVPPPSEAPPVP-KPSVGVPPVPPPSTAPPVPTPSAGLPPV 1229

Query: 849  PGLXXXKPP 875
            P      PP
Sbjct: 1230 PVPTAKAPP 1238


>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1461

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 16/55 (29%), Positives = 19/55 (34%)
 Frame = +3

Query: 687 PPXXPLXRPPXSXPXRLPEYLSXLSPFXXTVGXSPXPPPXXXSPXXGXXSXPPXP 851
           PP  P    P   P  +P     + P    +G  P PPP       G    PP P
Sbjct: 733 PPPPPAVIVPTPAPAPIP-----VPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782



 Score = 26.2 bits (55), Expect = 6.2
 Identities = 12/37 (32%), Positives = 14/37 (37%)
 Frame = +3

Query: 774 TVGXSPXPPPXXXSPXXGXXSXPPXPGLXXXKPPXXP 884
           T   +P P P       G    PP PG+    PP  P
Sbjct: 743 TPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779



 Score = 25.8 bits (54), Expect = 8.2
 Identities = 14/42 (33%), Positives = 16/42 (38%)
 Frame = +1

Query: 760 LPSGKPWXXPXXPPPXXFPXXRGXXPXPPXXGXXXXNPPFXP 885
           +P+  P   P  PP    P   G  P PP  G     PP  P
Sbjct: 741 VPTPAPAPIPVPPPA---PIMGGPPPPPPPPGVAGAGPPPPP 779


>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 309

 Score = 26.2 bits (55), Expect = 6.2
 Identities = 20/70 (28%), Positives = 22/70 (31%)
 Frame = +3

Query: 675 PGVSPPXXPLXRPPXSXPXRLPEYLSXLSPFXXTVGXSPXPPPXXXSPXXGXXSXPPXPG 854
           P  S P  P   PP   P   P   S   P    +  S  PP    +P     S P  P 
Sbjct: 139 PPTSAPPRPSIPPPS--PASAPPIPSKAPP----IPSSLPPPAQPAAPVKSPPSAPSLPS 192

Query: 855 LXXXKPPXXP 884
                PP  P
Sbjct: 193 AVPPMPPKVP 202


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,560,682
Number of Sequences: 5004
Number of extensions: 41499
Number of successful extensions: 123
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 92
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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