Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP15_F_H16
         (923 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   311   8e-86
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   307   1e-84
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   154   1e-38
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   130   4e-31
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   8.6  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   8.6  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  311 bits (764), Expect = 8e-86
 Identities = 149/248 (60%), Positives = 180/248 (72%)
 Frame = +3

Query: 150 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 329
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 330 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 509
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 510 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTX 689
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQVST 
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 690 VVEPYXSILTTXTTLEHSDCAFMGRQ*SHL*HLXP*SRH*APT*TNLKSSHRTXGSSITG 869
           VVEPY S+LTT  TL+ +DC FM    S             P+  NL        SSIT 
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240

Query: 870 SLKFXGAL 893
           SL+F G+L
Sbjct: 241 SLRFEGSL 248



 Score = 48.8 bits (111), Expect = 1e-06
 Identities = 27/54 (50%), Positives = 29/54 (53%)
 Frame = +1

Query: 760 VDNEAIYDIXRRNLDIERPPKPT*NRLIGXMAPXSLVL*NSTGXXNVALPXFQT 921
           VDNE+ YDI RRNLDIERP     NRLI  +           G  NV L  FQT
Sbjct: 204 VDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQT 257


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  307 bits (754), Expect = 1e-84
 Identities = 151/253 (59%), Positives = 183/253 (72%), Gaps = 5/253 (1%)
 Frame = +3

Query: 150 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 314
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 315 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 494
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 495 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 674
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 675 QVSTXVVEPYXSILTTXTTLEHSDCAFMGRQ*SHL*HLXP*SRH*APT*TNLKSSHRTXG 854
           QVST VVEPY S+LTT  TL++SDC FM    +             PT  NL        
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239

Query: 855 SSITGSLKFXGAL 893
           SSIT SL+F G+L
Sbjct: 240 SSITASLRFAGSL 252



 Score = 50.0 bits (114), Expect = 5e-07
 Identities = 28/54 (51%), Positives = 29/54 (53%)
 Frame = +1

Query: 760 VDNEAIYDIXRRNLDIERPPKPT*NRLIGXMAPXSLVL*NSTGXXNVALPXFQT 921
           VDNEA YDI RRNLDIERP     NRLI  +           G  NV L  FQT
Sbjct: 208 VDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQT 261


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  154 bits (374), Expect = 1e-38
 Identities = 77/202 (38%), Positives = 111/202 (54%)
 Frame = +3

Query: 150 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 329
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 330 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 509
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 510 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTX 689
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PAP+ S  
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 690 VVEPYXSILTTXTTLEHSDCAF 755
           VVEPY + L+    +E+SD  F
Sbjct: 179 VVEPYNATLSMHQLVENSDETF 200


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  130 bits (313), Expect = 4e-31
 Identities = 71/191 (37%), Positives = 109/191 (57%), Gaps = 3/191 (1%)
 Frame = +3

Query: 153 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 332
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 333 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 506
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 507 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 683
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179

Query: 684 TXVVEPYXSIL 716
             VV+PY S+L
Sbjct: 180 DVVVQPYNSLL 190


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 8.6
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +1

Query: 229 STASSLMARCPQTRPSGVETILSTLSSARPELAST 333
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 8.6
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +2

Query: 248 WPDAHRQDHRGWRRFFQHFLQR 313
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,544,639
Number of Sequences: 5004
Number of extensions: 71119
Number of successful extensions: 181
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 175
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 467341524
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)