Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP15_F_G23
(927 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 105 1e-23
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 83 4e-17
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 67 3e-12
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 54 2e-08
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 50 5e-07
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 50 5e-07
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 40 4e-04
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 33 0.043
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 31 0.30
SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyce... 26 8.7
SPBC14C8.17c |||SAGA complex subunit Spt8 |Schizosaccharomyces p... 26 8.7
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 105 bits (251), Expect = 1e-23
Identities = 48/105 (45%), Positives = 70/105 (66%), Gaps = 1/105 (0%)
Frame = +1
Query: 193 LSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLXKVDATQEQ 372
++K +I+ + ++V+FYAPWCGHCK+LAPEY AA +L E+ I L +VD T+E
Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADEL--EKDGISLVEVDCTEEG 84
Query: 373 DLXXSYXVRGYPTLKFFRNGSPI-XYSGGRQADDIIXWLKKKTGP 504
DL Y +RGYPTL F+NG I YSG R+ D ++ +++K+ P
Sbjct: 85 DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLP 129
Score = 87.0 bits (206), Expect = 3e-18
Identities = 43/111 (38%), Positives = 72/111 (64%), Gaps = 4/111 (3%)
Frame = +1
Query: 172 TEENVLXLSKANFETVI-STTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLX 348
++E+++ L NF+ ++ T+ +LVEFYAPWCGHCK+LAP Y K A + + ++S + +
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYS-DDSNVVVA 411
Query: 349 KVDATQEQDLXXSYXVRGYPTLKFFRNG---SPIXYSGGRQADDIIXWLKK 492
K+DAT E D+ S + G+PT+ FF+ +P+ Y G R +D+ ++ K
Sbjct: 412 KIDAT-ENDI--SVSISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 83.4 bits (197), Expect = 4e-17
Identities = 39/98 (39%), Positives = 60/98 (61%), Gaps = 2/98 (2%)
Frame = +1
Query: 214 TVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLXKVDATQEQDLXXSYX 393
T+ ++ + L+EFYA WCGHCKSLAP Y + L E+ + + + K+DA D+ Y
Sbjct: 34 TIRASKKGALIEFYATWCGHCKSLAPVYEELGA-LFEDHNDVLIGKIDADTHSDVADKYH 92
Query: 394 VRGYPTLKFF-RNGS-PIXYSGGRQADDIIXWLKKKTG 501
+ G+PTL +F +GS P+ YS R D + ++ +KTG
Sbjct: 93 ITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVSEKTG 130
Score = 79.8 bits (188), Expect = 5e-16
Identities = 40/111 (36%), Positives = 63/111 (56%), Gaps = 4/111 (3%)
Frame = +1
Query: 181 NVLXLSKANFETVISTTEY-ILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLXKVD 357
NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Y + K+ + E +++ K++
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY-ETLGKVFKNEPNVEIVKIN 199
Query: 358 ATQEQDLXXSYXVRGYPTLKFF---RNGSPIXYSGGRQADDIIXWLKKKTG 501
A D+ + V +PT+KFF P Y G R + +I ++ KK+G
Sbjct: 200 ADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 67.3 bits (157), Expect = 3e-12
Identities = 31/79 (39%), Positives = 42/79 (53%)
Frame = +1
Query: 181 NVLXLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLXKVDA 360
N + L+ NF + LV FYAPWCG+CK L P Y K A+ L P+ DA
Sbjct: 32 NTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTYQKLASNL-HSLLPVTAVDCDA 90
Query: 361 TQEQDLXXSYXVRGYPTLK 417
Q + + Y V+G+PT+K
Sbjct: 91 DQNRAVCSQYQVQGFPTIK 109
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 54.4 bits (125), Expect = 2e-08
Identities = 24/76 (31%), Positives = 42/76 (55%)
Frame = +1
Query: 214 TVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLXKVDATQEQDLXXSYX 393
+ I + Y+ V+ YA WCG CK+++P +++ A+K A + KV+ +++ +
Sbjct: 14 STIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYASPK--FVFAKVNVDEQRQIASGLG 71
Query: 394 VRGYPTLKFFRNGSPI 441
V+ PT FF NG I
Sbjct: 72 VKAMPTFVFFENGKQI 87
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 50.0 bits (114), Expect = 5e-07
Identities = 27/82 (32%), Positives = 39/82 (47%)
Frame = +1
Query: 196 SKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLXKVDATQEQD 375
S ++ T IS + +V+FYA WCG CK L P KL+E+ V+A + D
Sbjct: 24 SFGDYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQNQKASFIAVNADKFSD 79
Query: 376 LXXSYXVRGYPTLKFFRNGSPI 441
+ V PT+ FR G +
Sbjct: 80 IAQKNGVYALPTMVLFRKGQEL 101
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 50.0 bits (114), Expect = 5e-07
Identities = 21/80 (26%), Positives = 42/80 (52%)
Frame = +1
Query: 202 ANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLXKVDATQEQDLX 381
+ F++++ + ++V+F+A WCG CK++AP++ + + S KVD Q ++
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF----EQFSNTYSDATFIKVDVDQLSEIA 64
Query: 382 XSYXVRGYPTLKFFRNGSPI 441
V P+ ++NG I
Sbjct: 65 AEAGVHAMPSFFLYKNGEKI 84
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 40.3 bits (90), Expect = 4e-04
Identities = 27/91 (29%), Positives = 44/91 (48%), Gaps = 2/91 (2%)
Frame = +1
Query: 166 VPTEENVLXLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAE--EESPI 339
V T + + L+ + E+ +S + +++Y P CG CK L P + K E E S
Sbjct: 22 VQTLVSGVPLTDNDLESEVSKGTWF-IKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNF 80
Query: 340 KLXKVDATQEQDLXXSYXVRGYPTLKFFRNG 432
+VD ++E L +R PTL ++NG
Sbjct: 81 HFGEVDCSKE--LSSCANIRAVPTLYLYQNG 109
Score = 33.9 bits (74), Expect = 0.033
Identities = 17/108 (15%), Positives = 46/108 (42%)
Frame = +1
Query: 169 PTEENVLXLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLX 348
PT + A+ + ++ E ++FY+ C C ++ + A ++ + +
Sbjct: 278 PTGTSKALALDADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRM---RGKLNVA 334
Query: 349 KVDATQEQDLXXSYXVRGYPTLKFFRNGSPIXYSGGRQADDIIXWLKK 492
++ + Y ++ +PT FF+ + + Y G D++ + ++
Sbjct: 335 HINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEE 382
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 33.5 bits (73), Expect = 0.043
Identities = 16/59 (27%), Positives = 28/59 (47%)
Frame = +1
Query: 232 EYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLXKVDATQEQDLXXSYXVRGYP 408
+ IL+ FYAPW CK + + + A++ K++A + D+ S+ V P
Sbjct: 21 QIILLNFYAPWAAPCKQMNQVF----DQFAKDTKNAVFLKIEAEKFSDIAESFDVNAVP 75
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 30.7 bits (66), Expect = 0.30
Identities = 12/61 (19%), Positives = 31/61 (50%)
Frame = +1
Query: 271 HCKSLAPEYAKAATKLAEEESPIKLXKVDATQEQDLXXSYXVRGYPTLKFFRNGSPIXYS 450
HC+ A ++ + +K+ +V+ +E+++ + ++ +PT + F+ I Y+
Sbjct: 208 HCEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYN 267
Query: 451 G 453
G
Sbjct: 268 G 268
>SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 747
Score = 25.8 bits (54), Expect = 8.7
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = -2
Query: 116 NTHCDIIGTFLL 81
NTHCDI+ +FLL
Sbjct: 275 NTHCDIVTSFLL 286
>SPBC14C8.17c |||SAGA complex subunit Spt8 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 526
Score = 25.8 bits (54), Expect = 8.7
Identities = 15/39 (38%), Positives = 17/39 (43%)
Frame = +1
Query: 397 RGYPTLKFFRNGSPIXYSGGRQADDIIXWLKKKTGPPAC 513
RG L NG I YS Q + LKK T P +C
Sbjct: 199 RGLWVLSGVNNGDIILYSTRHQEGYPVTSLKKHTAPVSC 237
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,719,349
Number of Sequences: 5004
Number of extensions: 42842
Number of successful extensions: 106
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 93
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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