BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP15_F_E09
(905 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 34 0.032
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 25 0.44
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.91
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 1.2
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 28 1.6
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 24 2.0
SPBC660.05 |||conserved fungal protein|Schizosaccharomyces pombe... 28 2.1
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.8
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 3.7
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 24 4.7
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 4.8
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 26 8.5
SPAC26A3.08 |smb1|smb|Sm snRNP core protein Smb1|Schizosaccharom... 26 8.5
SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyc... 26 8.5
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 33.9 bits (74), Expect = 0.032
Identities = 15/31 (48%), Positives = 15/31 (48%), Gaps = 4/31 (12%)
Frame = +1
Query: 532 PPPPPXP----PXKXPPPPXXRGGGXXXPPP 612
P PPP P P PPPP G G PPP
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 29.9 bits (64), Expect = 0.52
Identities = 17/53 (32%), Positives = 20/53 (37%), Gaps = 5/53 (9%)
Frame = +1
Query: 532 PPPPPX-----PPXKXPPPPXXRGGGXXXPPPXRGGKXXXPXKKGGXTXXKKI 675
PPPPP PP PPPP GG P + + T +KI
Sbjct: 764 PPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEPEPKIDETSLTEEQKI 816
Score = 29.5 bits (63), Expect = 0.69
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +3
Query: 498 PPPPXKXXFXXSPPPPXPP---XKXPPPP 575
P PP PPPP PP PPPP
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPP 778
Score = 28.7 bits (61), Expect = 1.2
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +3
Query: 498 PPPPXKXXFXXSPPPPXPP 554
PPPP PPPP PP
Sbjct: 763 PPPPPPGVAGAGPPPPPPP 781
Score = 28.3 bits (60), Expect = 1.6
Identities = 12/25 (48%), Positives = 13/25 (52%)
Frame = +3
Query: 498 PPPPXKXXFXXSPPPPXPPXKXPPP 572
PPPP + PPP PP PPP
Sbjct: 762 PPPPPPPGVAGAGPPPPPP---PPP 783
Score = 27.9 bits (59), Expect = 2.1
Identities = 13/36 (36%), Positives = 13/36 (36%)
Frame = +1
Query: 532 PPPPPXPPXKXPPPPXXRGGGXXXPPPXRGGKXXXP 639
P P P P PP P G PPP G P
Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777
Score = 26.6 bits (56), Expect = 4.8
Identities = 13/38 (34%), Positives = 13/38 (34%)
Frame = +1
Query: 538 PPPXPPXKXPPPPXXRGGGXXXPPPXRGGKXXXPXKKG 651
PPP PP P P P P GG P G
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPG 769
Score = 26.2 bits (55), Expect = 6.4
Identities = 13/33 (39%), Positives = 14/33 (42%), Gaps = 7/33 (21%)
Frame = +3
Query: 498 PPPPXKXXFXXSP-------PPPXPPXKXPPPP 575
PPPP +P PPP P PPPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPP 764
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 24.6 bits (51), Expect(2) = 0.44
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = +1
Query: 532 PPPPPXPPXKXPP 570
P PPP PP PP
Sbjct: 236 PAPPPPPPPTLPP 248
Score = 23.8 bits (49), Expect(2) = 0.44
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = +1
Query: 523 FXXPPPPPXPP 555
+ PPPPP PP
Sbjct: 187 YNPPPPPPPPP 197
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.1 bits (62), Expect = 0.91
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +1
Query: 535 PPPPXPPXKXPPP---PXXRGGGXXXPPP 612
P PP PP PPP P G PPP
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPP 1733
Score = 29.1 bits (62), Expect = 0.91
Identities = 13/27 (48%), Positives = 13/27 (48%), Gaps = 1/27 (3%)
Frame = +3
Query: 498 PPPPXKXXFXXSPPP-PXPPXKXPPPP 575
PPPP S PP P P PPPP
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 28.3 bits (60), Expect = 1.6
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = +3
Query: 531 SPPPPXPPXKXPPPP 575
+P PP PP PPPP
Sbjct: 1704 APTPPPPPMSVPPPP 1718
Score = 26.6 bits (56), Expect = 4.8
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = +1
Query: 532 PPPPPXPPXKXPPPPXXRGGGXXXPPP 612
PPPP P PP G PPP
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 26.2 bits (55), Expect = 6.4
Identities = 10/26 (38%), Positives = 10/26 (38%)
Frame = +1
Query: 535 PPPPXPPXKXPPPPXXRGGGXXXPPP 612
PP P P PPPP P P
Sbjct: 1721 PPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/22 (54%), Positives = 12/22 (54%), Gaps = 3/22 (13%)
Frame = +1
Query: 532 PPPPPXP---PXKXPPPPXXRG 588
PPPPP P P PPPP G
Sbjct: 10 PPPPPPPGFEPPSQPPPPPPPG 31
Score = 27.5 bits (58), Expect = 2.8
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = +1
Query: 541 PPXPPXKXPPPPXXRGGGXXXPPPXRG 621
PP P PPPP PPP G
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPPG 31
Score = 27.5 bits (58), Expect = 2.8
Identities = 14/28 (50%), Positives = 15/28 (53%), Gaps = 2/28 (7%)
Frame = +3
Query: 498 PPPPXKXXFXXSPPPPX--PPXKXPPPP 575
PPPP PPPP PP + PPPP
Sbjct: 9 PPPP--------PPPPGFEPPSQPPPPP 28
Score = 27.5 bits (58), Expect = 2.8
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +3
Query: 498 PPPPXKXXFXXSPPPPXPP 554
PPPP PPPP PP
Sbjct: 12 PPPPPGFEPPSQPPPPPPP 30
Score = 26.6 bits (56), Expect = 4.8
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +3
Query: 498 PPPPXKXXFXXSPPPPXPP 554
PPPP S PPP PP
Sbjct: 11 PPPPPPGFEPPSQPPPPPP 29
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +1
Query: 535 PPPPX---PPXKXPPPPXXRGGGXXXPPP 612
PPPP P PPPP G PPP
Sbjct: 208 PPPPMHHKPGEHMPPPPMHHEPGEHMPPP 236
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +1
Query: 535 PPPPX---PPXKXPPPPXXRGGGXXXPPP 612
PPPP P PPPP G PPP
Sbjct: 221 PPPPMHHEPGEHMPPPPMHHEPGEHMPPP 249
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +1
Query: 535 PPPPX---PPXKXPPPPXXRGGGXXXPPP 612
PPPP P PPPP G PPP
Sbjct: 234 PPPPMHHEPGEHMPPPPMHHEPGEHMPPP 262
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +1
Query: 535 PPPPX---PPXKXPPPPXXRGGGXXXPPP 612
PPPP P PPPP G PPP
Sbjct: 247 PPPPMHHEPGEHMPPPPMHHEPGEHMPPP 275
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +1
Query: 535 PPPPX---PPXKXPPPPXXRGGGXXXPPP 612
PPPP P PPPP G PPP
Sbjct: 260 PPPPMHHEPGEHMPPPPMHHEPGEHMPPP 288
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +1
Query: 535 PPPPX---PPXKXPPPPXXRGGGXXXPPP 612
PPPP P PPPP G PPP
Sbjct: 273 PPPPMHHEPGEHMPPPPMHHEPGEHMPPP 301
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +1
Query: 535 PPPPX---PPXKXPPPPXXRGGGXXXPPP 612
PPPP P PPPP G PPP
Sbjct: 286 PPPPMHHEPGEHMPPPPMHHEPGEHMPPP 314
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +1
Query: 535 PPPPX---PPXKXPPPPXXRGGGXXXPPP 612
PPPP P PPPP G PPP
Sbjct: 299 PPPPMHHEPGEHMPPPPMHHEPGEHMPPP 327
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +1
Query: 535 PPPPX---PPXKXPPPPXXRGGGXXXPPP 612
PPPP P PPPP G PPP
Sbjct: 312 PPPPMHHEPGEHMPPPPMHHEPGEHMPPP 340
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 23.8 bits (49), Expect(2) = 2.0
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +1
Query: 538 PPPXPPXKXPPPPXXRGGGXXXPP 609
PPP PP PPP GG P
Sbjct: 945 PPPPPP---PPPLVSAAGGKFVSP 965
Score = 23.4 bits (48), Expect(2) = 5.6
Identities = 9/24 (37%), Positives = 9/24 (37%)
Frame = +1
Query: 541 PPXPPXKXPPPPXXRGGGXXXPPP 612
P PP PPPP G P
Sbjct: 942 PAFPPPPPPPPPLVSAAGGKFVSP 965
Score = 22.2 bits (45), Expect(2) = 2.0
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +1
Query: 523 FXXPPPPPXP 552
F PPPPP P
Sbjct: 944 FPPPPPPPPP 953
Score = 21.0 bits (42), Expect(2) = 5.6
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +1
Query: 532 PPPPPXPPXK 561
PPPPP P K
Sbjct: 907 PPPPPPLPVK 916
>SPBC660.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 143
Score = 27.9 bits (59), Expect = 2.1
Identities = 17/38 (44%), Positives = 17/38 (44%), Gaps = 3/38 (7%)
Frame = -2
Query: 637 GXXFSPPXGGGGXXP--PPRXXXGG-GGXFXGGXGGGG 533
G F P GGG P P GG GG GG GG G
Sbjct: 104 GPLFGPRIGGGHHGPLHGPHGGFGGRGGGRMGGRGGRG 141
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.5 bits (58), Expect = 2.8
Identities = 11/25 (44%), Positives = 12/25 (48%)
Frame = +1
Query: 538 PPPXPPXKXPPPPXXRGGGXXXPPP 612
PP PP + PP P G PPP
Sbjct: 1189 PPVPPPSEAPPVPKPSVGVPPVPPP 1213
Score = 27.1 bits (57), Expect = 3.7
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +1
Query: 535 PPPPXPPXKXPPPPXXRGGGXXXPPP 612
PP P P PP P G PPP
Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPPVPPP 1194
Score = 25.8 bits (54), Expect = 8.5
Identities = 11/25 (44%), Positives = 11/25 (44%), Gaps = 1/25 (4%)
Frame = +1
Query: 541 PPXP-PXKXPPPPXXRGGGXXXPPP 612
PP P P PP P G PPP
Sbjct: 1041 PPVPIPTSTPPVPKSSSGAPSAPPP 1065
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.1 bits (57), Expect = 3.7
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = +3
Query: 498 PPPPXKXXFXXSPPPPXPPXKXPPPP 575
PPPP + S P P PPPP
Sbjct: 339 PPPPPRSNAAGSIPLPPQGRSAPPPP 364
Score = 27.1 bits (57), Expect = 3.7
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +3
Query: 498 PPPPXKXXFXXSPPPPXPPXKXPPPP 575
PPPP P PP PPPP
Sbjct: 340 PPPPRSNAAGSIPLPPQGRSAPPPPP 365
Score = 25.8 bits (54), Expect = 8.5
Identities = 10/27 (37%), Positives = 11/27 (40%)
Frame = +1
Query: 532 PPPPPXPPXKXPPPPXXRGGGXXXPPP 612
PPPPP + PP PPP
Sbjct: 313 PPPPPSRRNRGKPPIGNGSSNSSLPPP 339
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 23.8 bits (49), Expect(2) = 4.7
Identities = 9/18 (50%), Positives = 9/18 (50%)
Frame = +1
Query: 532 PPPPPXPPXKXPPPPXXR 585
PPPPP PP P R
Sbjct: 370 PPPPPPPPELLNHSPKSR 387
Score = 21.0 bits (42), Expect(2) = 4.7
Identities = 6/10 (60%), Positives = 7/10 (70%)
Frame = +1
Query: 523 FXXPPPPPXP 552
+ PPPPP P
Sbjct: 368 YSPPPPPPPP 377
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 26.6 bits (56), Expect = 4.8
Identities = 19/48 (39%), Positives = 19/48 (39%), Gaps = 1/48 (2%)
Frame = -2
Query: 637 GXXFSPPXGGGGXXPPPRXXXGGGGXFXG-GXGGGGEXKKXLFXGGGG 497
G F GG G PP GG G F G G GG F GG G
Sbjct: 187 GGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGG---FGGGPG 231
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 25.8 bits (54), Expect = 8.5
Identities = 15/32 (46%), Positives = 15/32 (46%)
Frame = -2
Query: 628 FSPPXGGGGXXPPPRXXXGGGGXFXGGXGGGG 533
FS P GGG R GG G F G G GG
Sbjct: 440 FSTPRTGGGSR-GGRGGFGGRGGFGGRGGFGG 470
>SPAC26A3.08 |smb1|smb|Sm snRNP core protein
Smb1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 147
Score = 25.8 bits (54), Expect = 8.5
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = -3
Query: 624 PPPXGGGXGXPPP 586
PPP G G G PPP
Sbjct: 130 PPPAGFGRGAPPP 142
>SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 379
Score = 25.8 bits (54), Expect = 8.5
Identities = 17/44 (38%), Positives = 17/44 (38%), Gaps = 3/44 (6%)
Frame = -2
Query: 610 GGGXXPPPRXXXGGGGXFXG--GXGGGGEXKKXLFXGG-GGXXF 488
G PPP G G F G G G GG GG GG F
Sbjct: 72 GNAAPPPPGAEGGPGAGFGGFPGAGPGGARTFHFNMGGPGGAQF 115
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,546,519
Number of Sequences: 5004
Number of extensions: 20262
Number of successful extensions: 335
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 47
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 458501510
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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