Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP15_F_E06
(891 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 186 4e-48
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 97 3e-21
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 95 1e-20
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 94 2e-20
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 59 7e-10
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 56 7e-09
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 47 4e-06
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 33 0.041
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.51
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 29 1.2
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.3
SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 26 6.3
SPBC19C7.08c |||leucine carboxyl methyltransferase|Schizosacchar... 26 8.3
SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1 |... 26 8.3
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 186 bits (453), Expect = 4e-48
Identities = 81/88 (92%), Positives = 86/88 (97%)
Frame = +3
Query: 246 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHP 425
+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP
Sbjct: 43 IMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP 102
Query: 426 VLLTEAPLNPKANREKMTQIMFETFNSP 509
LLTEAPLNPK+NREKMTQI+FETFN+P
Sbjct: 103 CLLTEAPLNPKSNREKMTQIIFETFNAP 130
Score = 93.9 bits (223), Expect = 3e-20
Identities = 47/57 (82%), Positives = 50/57 (87%)
Frame = +1
Query: 508 PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYQGYALAHAHPPVWDLAGXDL 678
PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIY+GYAL HA + DLAG DL
Sbjct: 130 PAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRL-DLAGRDL 185
Score = 84.2 bits (199), Expect = 2e-17
Identities = 35/41 (85%), Positives = 40/41 (97%)
Frame = +2
Query: 122 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG 244
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHG 42
Score = 29.5 bits (63), Expect = 0.67
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = +2
Query: 680 TDYLMKILTEXGYSF 724
TDYLMKIL E GY+F
Sbjct: 186 TDYLMKILMERGYTF 200
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 97.1 bits (231), Expect = 3e-21
Identities = 45/89 (50%), Positives = 60/89 (67%), Gaps = 1/89 (1%)
Frame = +3
Query: 246 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEH 422
VM QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + + +L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 423 PVLLTEAPLNPKANREKMTQIMFETFNSP 509
P+LLTE PL N+EK+ + +ET N P
Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVP 135
Score = 62.1 bits (144), Expect = 1e-10
Identities = 26/45 (57%), Positives = 37/45 (82%)
Frame = +1
Query: 508 PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYQGYALAHA 642
PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY G+++ A
Sbjct: 135 PALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSA 179
Score = 52.8 bits (121), Expect = 6e-08
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +2
Query: 128 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 241
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 95.1 bits (226), Expect = 1e-20
Identities = 39/82 (47%), Positives = 60/82 (73%)
Frame = +3
Query: 267 KDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 446
KD VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 447 LNPKANREKMTQIMFETFNSPG 512
+NP ANREKM + MFE + G
Sbjct: 110 MNPVANREKMCETMFERYGFGG 131
Score = 66.9 bits (156), Expect = 4e-12
Identities = 32/57 (56%), Positives = 42/57 (73%)
Frame = +1
Query: 514 MYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYQGYALAHAHPPVWDLAGXDLNR 684
+YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+Y+ L H + D+AG D R
Sbjct: 132 VYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRL-DVAGRDATR 187
Score = 46.8 bits (106), Expect = 4e-06
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +2
Query: 134 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 232
A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 94.3 bits (224), Expect = 2e-20
Identities = 48/113 (42%), Positives = 65/113 (57%), Gaps = 4/113 (3%)
Frame = +3
Query: 186 ATTRPAPSSRPSWVALATRXVMVGMGQK---DSYVGDEAQSKRGI-LTLKYPIEHGIITN 353
A++ PA SS+PS++A + D ++G++A K +L YPI HG I N
Sbjct: 42 ASSGPAVSSKPSYMASKGSGHLSSKRATEDLDFFIGNDALKKASAGYSLDYPIRHGQIEN 101
Query: 354 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPG 512
WD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE+FN G
Sbjct: 102 WDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNCAG 154
Score = 47.2 bits (107), Expect = 3e-06
Identities = 26/63 (41%), Positives = 40/63 (63%), Gaps = 8/63 (12%)
Frame = +1
Query: 514 MYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTVPIYQGYALAHAHPPVWDLAG 669
+Y+A+QAVL+L AS + TG V+DSGDGV+H +P+ +GY + + + LAG
Sbjct: 155 LYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSIKTM-PLAG 213
Query: 670 XDL 678
D+
Sbjct: 214 RDV 216
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +2
Query: 140 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 223
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 59.3 bits (137), Expect = 7e-10
Identities = 28/85 (32%), Positives = 51/85 (60%), Gaps = 1/85 (1%)
Frame = +3
Query: 258 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 434
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 435 TEAPLNPKANREKMTQIMFETFNSP 509
TE NP R MT+++FE +N+P
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAP 145
Score = 37.5 bits (83), Expect = 0.003
Identities = 21/58 (36%), Positives = 34/58 (58%)
Frame = +2
Query: 62 AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 235
A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
Score = 29.9 bits (64), Expect = 0.51
Identities = 13/41 (31%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
Frame = +1
Query: 508 PAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYQG 624
P++ I + S Y + + ++GIVL+ G+ SH +P+ G
Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNG 185
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 56.0 bits (129), Expect = 7e-09
Identities = 24/66 (36%), Positives = 36/66 (54%)
Frame = +3
Query: 312 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 491
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 492 ETFNSP 509
E+ P
Sbjct: 123 ESLRCP 128
Score = 41.5 bits (93), Expect = 2e-04
Identities = 23/60 (38%), Positives = 35/60 (58%), Gaps = 2/60 (3%)
Frame = +2
Query: 125 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGPERLLRG*RGPE 298
D+V+A+V+D GS + GF+G+D P+ V PS G + DGR +G E + + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYIYKSNPGME 64
Score = 33.5 bits (73), Expect = 0.041
Identities = 19/58 (32%), Positives = 29/58 (50%)
Frame = +1
Query: 508 PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYQGYALAHAHPPVWDLAGXDLN 681
PA Y+A Q + +ASG+ T ++D G S IY G+ L + V +G +N
Sbjct: 128 PATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGY-QVQHFSGNAIN 184
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 46.8 bits (106), Expect = 4e-06
Identities = 24/83 (28%), Positives = 44/83 (53%), Gaps = 2/83 (2%)
Frame = +3
Query: 267 KDSYVGDEAQSKRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 440
++ YV DE Q I + +K +GII +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 441 APLNPKANREKMTQIMFETFNSP 509
NP++ R+++ + FE + P
Sbjct: 109 PSWNPQSVRQQIMEAAFEQLHVP 131
Score = 34.3 bits (75), Expect = 0.024
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +2
Query: 125 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 226
+++ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 33.5 bits (73), Expect = 0.041
Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 4/66 (6%)
Frame = +3
Query: 327 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 494
PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 495 TFNSPG 512
PG
Sbjct: 172 ECQVPG 177
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +1
Query: 508 PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYQG 624
P +A + ++ LYA G G+V+D G + PI G
Sbjct: 176 PGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDG 214
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 29.9 bits (64), Expect = 0.51
Identities = 14/43 (32%), Positives = 26/43 (60%), Gaps = 2/43 (4%)
Frame = +3
Query: 387 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSP 509
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 28.7 bits (61), Expect = 1.2
Identities = 13/30 (43%), Positives = 21/30 (70%), Gaps = 1/30 (3%)
Frame = +3
Query: 429 LLTEAPLNPKANREKMTQIMFETFN-SPGH 515
+L APL+ + +KM +++FET+N S GH
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYNESDGH 210
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.3
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -2
Query: 350 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHHXPG 237
GDD+VLD V +D L L AH D H G
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAG 713
>SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 238
Score = 26.2 bits (55), Expect = 6.3
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = -2
Query: 554 EAYSESTAWMATYMAGGVEGLKHDLGHLLPVGLGVE 447
E +++ A +A Y+A ++GL H + LL LG+E
Sbjct: 155 EGLAKAIAGLAKYVAKAIQGLAHIILSLLLFILGLE 190
>SPBC19C7.08c |||leucine carboxyl
methyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 681
Score = 25.8 bits (54), Expect = 8.3
Identities = 12/28 (42%), Positives = 13/28 (46%)
Frame = -3
Query: 685 VGSSRDQPSPRPEDGRGPERNLGRWVRC 602
VG S QP + R P N G W RC
Sbjct: 40 VGHSYYQPFIQKSPRRSPSVNRGYWTRC 67
>SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 25.8 bits (54), Expect = 8.3
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = -2
Query: 581 SSTIPVVRPEAYSESTAWMATYMAGGVEGLK 489
+ TI V P + WM M GG+EG++
Sbjct: 518 AETIQCVLPIRIEKRVVWMEDGMEGGMEGMQ 548
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,117,988
Number of Sequences: 5004
Number of extensions: 62453
Number of successful extensions: 212
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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