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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP15_F_B06
         (895 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit...    72   1e-13
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po...    66   8e-12
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta...    28   2.1  
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ...    27   2.7  
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch...    27   3.6  
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces...    27   3.6  
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ...    26   6.3  
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo...    26   6.3  
SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog...    26   8.3  

>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 72.1 bits (169), Expect = 1e-13
 Identities = 48/159 (30%), Positives = 81/159 (50%), Gaps = 4/159 (2%)
 Frame = +3

Query: 180 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN----ESLRGGDLATQARVWQW 347
           N   D   KFP  K+P F   DG   L+E+ AIA+Y+A+     +L G     +A+V Q+
Sbjct: 38  NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQY 96

Query: 348 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERXT 527
            S+++SEL  A    + P +    +++Q  + A++ +       D  L ++T+LV  R T
Sbjct: 97  CSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLT 156

Query: 528 LADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQ 644
           LAD+     L     +VL  S  +   ++ R++ T+ HQ
Sbjct: 157 LADIFFTCFLKFGATYVLTKSYLAKYTHIYRYYQTIYHQ 195


>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 220

 Score = 65.7 bits (153), Expect = 8e-12
 Identities = 51/203 (25%), Positives = 93/203 (45%), Gaps = 8/203 (3%)
 Frame = +3

Query: 60  MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 239
           M  G LY++  N R    L  A+     V +   +       S D   KFP  K+P F  
Sbjct: 1   MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIG 57

Query: 240 ADGKVLLTESNAIAYYVAN------ESLRGGDLATQARVWQWASWSDSELL-PASCA-WV 395
           ADG  L      + Y+         E L   +   +A + +W  + + +++ P +   WV
Sbjct: 58  ADGFELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWV 117

Query: 396 FPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERXTLADVIVFSTLLHAFQH 575
             + G + + ++  + + +  + +LK+ +  +  RT+LV +R TLAD+   S L   F  
Sbjct: 118 GMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADLFFGSLLRIFFNS 177

Query: 576 VLDPSVRSSLINVQRWFLTVAHQ 644
           ++D   R  L ++ R+++T+ HQ
Sbjct: 178 IIDEKTRKELPHLTRYYITMFHQ 200


>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 450

 Score = 27.9 bits (59), Expect = 2.1
 Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
 Frame = +3

Query: 477 LDGHLLTRTFLVTERX-TLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQ 644
           LD  L + TF+  +   ++AD+ V++ + H++   L       L NV RWF  + HQ
Sbjct: 128 LDEFLKSSTFIAQDSGISVADLAVYARI-HSYICGLSAKEGYKLNNVCRWFDFIQHQ 183


>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 267

 Score = 27.5 bits (58), Expect = 2.7
 Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
 Frame = +1

Query: 316 IWLPKPVSGSGHHGLTVNYCL-LPVLGSSLTLV 411
           +W+P PV  SG    T+  C+ LP  G  +T V
Sbjct: 23  LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55


>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
           Eme1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 738

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 16/62 (25%), Positives = 30/62 (48%)
 Frame = +3

Query: 87  PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 266
           P  F++ KA +  QY+          +FG  +K ++F K+    ++  F S +  +L+  
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611

Query: 267 SN 272
           SN
Sbjct: 612 SN 613


>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 442

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 13/31 (41%), Positives = 16/31 (51%)
 Frame = +1

Query: 454 TYWPP*KYWTDIFSHAPSLLPRXSHLPMSLS 546
           TY PP  Y  D+FS APS+         S+S
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287


>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 369

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = -1

Query: 256 STFPSALSNAGTFPAGNFFKKSSDL 182
           +TFP A +  GTFP G  F   S L
Sbjct: 52  ATFPWAQAPVGTFPIGQLFDPVSGL 76


>SPBC16A3.11 |eso1||sister chromatid cohesion protein
           Eso1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 872

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 12/29 (41%), Positives = 18/29 (62%)
 Frame = +3

Query: 267 SNAIAYYVANESLRGGDLATQARVWQWAS 353
           S + A Y+ NE+LR G +   +R+W  AS
Sbjct: 780 STSSAVYIKNENLRKGFVLGISRIWVSAS 808


>SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 982

 Score = 25.8 bits (54), Expect = 8.3
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = +1

Query: 364 VNYCLLPVLGSSLTLVSCNSTNRML 438
           V + L+P LG  L + SCN+  R L
Sbjct: 259 VTHALVPELGLQLAMASCNALLRYL 283


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,388,367
Number of Sequences: 5004
Number of extensions: 68236
Number of successful extensions: 207
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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