BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP15_F_B06
(895 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 72 1e-13
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 66 8e-12
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 28 2.1
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ... 27 2.7
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 27 3.6
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 27 3.6
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ... 26 6.3
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo... 26 6.3
SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog... 26 8.3
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 72.1 bits (169), Expect = 1e-13
Identities = 48/159 (30%), Positives = 81/159 (50%), Gaps = 4/159 (2%)
Frame = +3
Query: 180 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN----ESLRGGDLATQARVWQW 347
N D KFP K+P F DG L+E+ AIA+Y+A+ +L G +A+V Q+
Sbjct: 38 NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQY 96
Query: 348 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERXT 527
S+++SEL A + P + +++Q + A++ + D L ++T+LV R T
Sbjct: 97 CSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLT 156
Query: 528 LADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQ 644
LAD+ L +VL S + ++ R++ T+ HQ
Sbjct: 157 LADIFFTCFLKFGATYVLTKSYLAKYTHIYRYYQTIYHQ 195
>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 220
Score = 65.7 bits (153), Expect = 8e-12
Identities = 51/203 (25%), Positives = 93/203 (45%), Gaps = 8/203 (3%)
Frame = +3
Query: 60 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 239
M G LY++ N R L A+ V + + S D KFP K+P F
Sbjct: 1 MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIG 57
Query: 240 ADGKVLLTESNAIAYYVAN------ESLRGGDLATQARVWQWASWSDSELL-PASCA-WV 395
ADG L + Y+ E L + +A + +W + + +++ P + WV
Sbjct: 58 ADGFELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWV 117
Query: 396 FPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERXTLADVIVFSTLLHAFQH 575
+ G + + ++ + + + + +LK+ + + RT+LV +R TLAD+ S L F
Sbjct: 118 GMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADLFFGSLLRIFFNS 177
Query: 576 VLDPSVRSSLINVQRWFLTVAHQ 644
++D R L ++ R+++T+ HQ
Sbjct: 178 IIDEKTRKELPHLTRYYITMFHQ 200
>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 27.9 bits (59), Expect = 2.1
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +3
Query: 477 LDGHLLTRTFLVTERX-TLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQ 644
LD L + TF+ + ++AD+ V++ + H++ L L NV RWF + HQ
Sbjct: 128 LDEFLKSSTFIAQDSGISVADLAVYARI-HSYICGLSAKEGYKLNNVCRWFDFIQHQ 183
>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 267
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = +1
Query: 316 IWLPKPVSGSGHHGLTVNYCL-LPVLGSSLTLV 411
+W+P PV SG T+ C+ LP G +T V
Sbjct: 23 LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55
>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
Eme1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 27.1 bits (57), Expect = 3.6
Identities = 16/62 (25%), Positives = 30/62 (48%)
Frame = +3
Query: 87 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 266
P F++ KA + QY+ +FG +K ++F K+ ++ F S + +L+
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611
Query: 267 SN 272
SN
Sbjct: 612 SN 613
>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 442
Score = 27.1 bits (57), Expect = 3.6
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +1
Query: 454 TYWPP*KYWTDIFSHAPSLLPRXSHLPMSLS 546
TY PP Y D+FS APS+ S+S
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287
>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 369
Score = 26.2 bits (55), Expect = 6.3
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -1
Query: 256 STFPSALSNAGTFPAGNFFKKSSDL 182
+TFP A + GTFP G F S L
Sbjct: 52 ATFPWAQAPVGTFPIGQLFDPVSGL 76
>SPBC16A3.11 |eso1||sister chromatid cohesion protein
Eso1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 26.2 bits (55), Expect = 6.3
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +3
Query: 267 SNAIAYYVANESLRGGDLATQARVWQWAS 353
S + A Y+ NE+LR G + +R+W AS
Sbjct: 780 STSSAVYIKNENLRKGFVLGISRIWVSAS 808
>SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 982
Score = 25.8 bits (54), Expect = 8.3
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +1
Query: 364 VNYCLLPVLGSSLTLVSCNSTNRML 438
V + L+P LG L + SCN+ R L
Sbjct: 259 VTHALVPELGLQLAMASCNALLRYL 283
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,388,367
Number of Sequences: 5004
Number of extensions: 68236
Number of successful extensions: 207
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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