Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP14_F_O02
(844 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 64 3e-11
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 44 4e-05
SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity... 42 8e-05
SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po... 39 8e-04
SPAC22E12.02 |||RNA-binding protein|Schizosaccharomyces pombe|ch... 34 0.029
SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc... 33 0.051
SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccha... 33 0.067
SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 31 0.27
SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces po... 30 0.47
SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|... 29 0.62
SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|c... 29 1.1
SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyc... 27 2.5
SPAC343.15 |||tRNA isopentenyltransferase|Schizosaccharomyces po... 27 3.3
SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomy... 27 4.4
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 26 5.8
SPAC15A10.15 |sgo2||shugoshin Sgo2|Schizosaccharomyces pombe|chr... 26 5.8
SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.8
SPAC4G9.04c |||cleavage and polyadenylation specificity factor |... 26 5.8
SPAC6F6.17 |rif1|tap1, tap11, SPAPJ736.01|telomere length regula... 26 7.7
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 63.7 bits (148), Expect = 3e-11
Identities = 42/112 (37%), Positives = 59/112 (52%), Gaps = 5/112 (4%)
Frame = +1
Query: 220 SHTRAPVVLPSASRAARG---GLAVDDESIPHRPPFIAHISNLPYDVEESAIAELFADLK 390
S T V P SR G G D IP PPF AH+ NL +D+ E+ + + F +
Sbjct: 59 SSTFESVRSPPESRREGGMGSGYQRDAIPIPSEPPFTAHVGNLSFDLTENDLGDFFGE-G 117
Query: 391 VTNLRLPRE--GDRLKGHGYVDFEDRENLIEALNMPDLTIGGRRVRIEVSTP 540
VT++RL + +R +G GYV+FE + L AL + + GR VRI V+ P
Sbjct: 118 VTSIRLVIDPLTERSRGFGYVEFETADTLSAALALSGEDLMGRPVRITVAEP 169
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 43.6 bits (98), Expect = 4e-05
Identities = 25/80 (31%), Positives = 40/80 (50%), Gaps = 3/80 (3%)
Frame = +1
Query: 310 PPFIAHISNLPYDVEESAIAELFADL-KVTNLRLPRE--GDRLKGHGYVDFEDRENLIEA 480
P + NL ++ E ++ F + ++RLP + RLKG GYV F D ++ +
Sbjct: 364 PSDTVFVGNLSFNATEDDLSTAFGGCGDIQSIRLPTDPQSGRLKGFGYVTFSDIDSAKKC 423
Query: 481 LNMPDLTIGGRRVRIEVSTP 540
+ M I GR R++ STP
Sbjct: 424 VEMNGHFIAGRPCRLDFSTP 443
Score = 33.1 bits (72), Expect = 0.051
Identities = 22/75 (29%), Positives = 38/75 (50%), Gaps = 4/75 (5%)
Frame = +1
Query: 328 ISNLPYDVEESAIAELFADL-KVTNLRLPREGD--RLKGHGYVDFEDRENLIEALNMPDL 498
+ L ++V++ + + F + + R+ +G R KG+GYVDFE E A+
Sbjct: 267 VGRLSWNVDDQWLGQEFEEYGTIVGARVIMDGQSGRSKGYGYVDFETPEAAKAAVAANGT 326
Query: 499 -TIGGRRVRIEVSTP 540
I GR V +++S P
Sbjct: 327 KEIDGRMVNLDLSNP 341
>SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity
factor complex subunit Ctf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 363
Score = 42.3 bits (95), Expect = 8e-05
Identities = 23/74 (31%), Positives = 39/74 (52%), Gaps = 4/74 (5%)
Frame = +1
Query: 319 IAHISNLPYDVEESAIAELF---ADLKVTNLRLPREGDRLKGHGYVDFEDRENLIEAL-N 486
+ + N+PYDV E + E+F +K L L E KG+G+ +F D E A+
Sbjct: 8 VVFVGNIPYDVSEQQMTEIFNQVGPVKTFKLVLDPETGSGKGYGFCEFFDSETTAMAVRK 67
Query: 487 MPDLTIGGRRVRIE 528
+ + +G R++R+E
Sbjct: 68 LNNSELGPRKIRVE 81
>SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1014
Score = 39.1 bits (87), Expect = 8e-04
Identities = 19/73 (26%), Positives = 39/73 (53%), Gaps = 1/73 (1%)
Frame = +1
Query: 325 HISNLPYDVEESAIAELFADL-KVTNLRLPREGDRLKGHGYVDFEDRENLIEALNMPDLT 501
+++N+ + V E + F D +V ++R+P+ ++ KG GYV ++ AL+
Sbjct: 760 YVTNIDFKVNEKDVETFFRDYGQVESVRIPKRFNQHKGFGYVVMTTNQDAENALSAAGKQ 819
Query: 502 IGGRRVRIEVSTP 540
+G R + + +S P
Sbjct: 820 LGNRVLNVVLSKP 832
>SPAC22E12.02 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 219
Score = 33.9 bits (74), Expect = 0.029
Identities = 26/95 (27%), Positives = 48/95 (50%), Gaps = 6/95 (6%)
Frame = +1
Query: 271 GGLAVDDESIPHRPP--FIAHISNLPYDVEESAIAELFAD---LKVTNLRLPREGDRLKG 435
GG +D ++ P F + NL DV + ++ + F++ L T + REG + +G
Sbjct: 13 GGQVWEDPTLLEWDPNHFRLFVGNLGNDVNDESLYQAFSEYPSLVKTKVVRDREG-KTRG 71
Query: 436 HGYVDFEDRENLIEALNMPD-LTIGGRRVRIEVST 537
G+V F+D + ++A + IG R V++ +T
Sbjct: 72 FGFVSFKDSDQFLKAWREKNGKYIGSRPVKLSRAT 106
>SPAC328.05 |||RNA-binding protein involved in export of
mRNAs|Schizosaccharomyces pombe|chr 1|||Manual
Length = 434
Score = 33.1 bits (72), Expect = 0.051
Identities = 27/81 (33%), Positives = 39/81 (48%), Gaps = 6/81 (7%)
Frame = +1
Query: 301 PHRP-PFIAHISNLPYDVEESAIAELFADL-KVTNLRLPRE-GDRLKGHGYVDFE---DR 462
PH P ++ NLP+ + + +LF D+ V R+ E R KG G V FE D
Sbjct: 274 PHGPCSDCIYVGNLPWATSDRNLLDLFTDIGSVIRARIAYEPTGRSKGFGVVQFENENDA 333
Query: 463 ENLIEALNMPDLTIGGRRVRI 525
+ IE LN GGR +++
Sbjct: 334 ASSIEKLN--GYRYGGRPLQL 352
>SPBC660.15 |||mRNA cleavage factor complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 474
Score = 32.7 bits (71), Expect = 0.067
Identities = 19/74 (25%), Positives = 36/74 (48%), Gaps = 3/74 (4%)
Frame = +1
Query: 328 ISNLPYDVEESAIAELFADL-KVTNLRLPREGD--RLKGHGYVDFEDRENLIEALNMPDL 498
+ +P D E F +V + L + D R +G G+V +E+ + ++ P +
Sbjct: 251 VGGVPGDCTEEEFRNFFNQFGRVLDATLMMDKDTGRPRGFGFVTYENESAVEATMSQPYI 310
Query: 499 TIGGRRVRIEVSTP 540
TI G+ V ++ +TP
Sbjct: 311 TIHGKPVEVKRATP 324
>SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 674
Score = 30.7 bits (66), Expect = 0.27
Identities = 25/79 (31%), Positives = 44/79 (55%), Gaps = 4/79 (5%)
Frame = +1
Query: 304 HRPPFIAHISNLPYDVEESAIAEL-FADL-KVTNLRLPREGD-RLKGHGYVDFEDRENLI 474
+RP I I NLP+ +++ E F+ KV +++P +G R+ G +V +DR+
Sbjct: 105 NRPRLI--IRNLPWSIKKPQHLEPHFSKFGKVREIKIPTKGGGRMCGFAFVWMKDRKAAE 162
Query: 475 EALNMPDLT-IGGRRVRIE 528
EA+N + T I GR + ++
Sbjct: 163 EAMNSLNGTEIDGRPIAVD 181
>SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 833
Score = 29.9 bits (64), Expect = 0.47
Identities = 14/54 (25%), Positives = 27/54 (50%), Gaps = 3/54 (5%)
Frame = +1
Query: 328 ISNLPYDVEESAIAELFA---DLKVTNLRLPREGDRLKGHGYVDFEDRENLIEA 480
+ NL Y E + LF L+ ++ + ++ + KG Y+DF D ++ + A
Sbjct: 327 LRNLTYSCAEDDLKSLFGPFGQLEQVHMPIDKKTNNPKGFAYIDFHDADDAVRA 380
Score = 25.8 bits (54), Expect = 7.7
Identities = 10/35 (28%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
Frame = +1
Query: 433 GHGYVDFEDRENLIEALN-MPDLTIGGRRVRIEVS 534
G G+V+F+D+ + + A++ M + G ++ I++S
Sbjct: 666 GFGFVEFKDKASAVAAMHAMNGFVLDGHKLEIKLS 700
>SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 330
Score = 29.5 bits (63), Expect = 0.62
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = -2
Query: 132 CDVGFDPFCLL*LLACSGHIKRLGL 58
CD G D L L AC+GH K LG+
Sbjct: 23 CDPGHDDVVALTLAACAGHCKILGV 47
>SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 489
Score = 28.7 bits (61), Expect = 1.1
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -2
Query: 570 ASTSSHSTVIWSRHLNSY 517
AS +S ST+ W HLNSY
Sbjct: 250 ASFASQSTIAWQSHLNSY 267
>SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 434
Score = 27.5 bits (58), Expect = 2.5
Identities = 22/71 (30%), Positives = 30/71 (42%)
Frame = +1
Query: 316 FIAHISNLPYDVEESAIAELFADLKVTNLRLPREGDRLKGHGYVDFEDRENLIEALNMPD 495
F+ +I DV + +F +K E R KG YVDF + E + ALN
Sbjct: 318 FVKNIPPETSDVSLKSAMSIFGPVKAI------EFARRKGTAYVDFVNHECVQLALNKKT 371
Query: 496 LTIGGRRVRIE 528
L I + IE
Sbjct: 372 LQINNATLNIE 382
>SPAC343.15 |||tRNA isopentenyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 434
Score = 27.1 bits (57), Expect = 3.3
Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = +1
Query: 355 ESAIAELFADLKVTNLRLPREGDRLKGHGYVD-FEDRENLIEALNM-PDLTIG 507
+S I FAD V RL + D++ HG VD + ++L E+ PD T G
Sbjct: 201 KSLIFWAFADSLVLMPRLDKRVDKMLSHGLVDEIKSMKSLAESEKFSPDFTRG 253
>SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 436
Score = 26.6 bits (56), Expect = 4.4
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +2
Query: 551 EWDEVDALIEIGTMTLSVQWVTGAPDPALYP 643
++ ++DA I++ LS+ W P P L P
Sbjct: 356 QFSDIDAWIQVACPRLSIDWGYAFPAPLLTP 386
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 26.2 bits (55), Expect = 5.8
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 530 CLLQMTVEWDEVDALIEIGTMTLSVQWVTGAPDP 631
CL + +W ++I T+ LS+Q + GAP+P
Sbjct: 86 CLSTLKKDWSPA---LQIRTVLLSIQALMGAPNP 116
>SPAC15A10.15 |sgo2||shugoshin Sgo2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 647
Score = 26.2 bits (55), Expect = 5.8
Identities = 23/78 (29%), Positives = 33/78 (42%), Gaps = 13/78 (16%)
Frame = +1
Query: 235 PVVLPSASRAARGGLAVDDESIPHRPPFIAHISNL---PYDVEESAIAELFADL------ 387
P+ L S ++ G + P RPP + I N PY++E S + D
Sbjct: 456 PMRLSSEPPSSNGFSIAHPNNSPLRPPSLQGILNAEDRPYEIEPSRSSFATNDTGSYNNL 515
Query: 388 ----KVTNLRLPREGDRL 429
VTNL+ P E DR+
Sbjct: 516 ELLSSVTNLKSPNENDRV 533
>SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 262
Score = 26.2 bits (55), Expect = 5.8
Identities = 23/86 (26%), Positives = 40/86 (46%), Gaps = 1/86 (1%)
Frame = -3
Query: 383 SANNSAIALSSTS*GRLEMCAINGGRCGIDSSSTAKP-PRAAREAEGSTTGARVCDAYPK 207
SA++S + S++S G + + + S+ST + A S G +Y
Sbjct: 178 SASSSVSSSSASSSGSIS--SADAKTVSASSNSTISGFSTSTTSASSSAAGNSSSSSYTS 235
Query: 206 FSVSSSAAFAQLLVVAIVVGSAIMLV 129
+S + S AQL V A + +A+ML+
Sbjct: 236 YSGAVSNGVAQLSVAACMGIAALMLI 261
>SPAC4G9.04c |||cleavage and polyadenylation specificity factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 638
Score = 26.2 bits (55), Expect = 5.8
Identities = 15/47 (31%), Positives = 23/47 (48%)
Frame = +1
Query: 295 SIPHRPPFIAHISNLPYDVEESAIAELFADLKVTNLRLPREGDRLKG 435
S+ PP + +N E S+I LFA+L+ L P G + +G
Sbjct: 336 SLSSGPPASLYNANALTPEESSSIDSLFANLQAAGLVPPSAGGKSQG 382
>SPAC6F6.17 |rif1|tap1, tap11, SPAPJ736.01|telomere length regulator
protein Rif1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1400
Score = 25.8 bits (54), Expect = 7.7
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +2
Query: 515 EYELRCLLQMTVEWDEVDALIEIGTMTLS 601
EY LR L++ + WD V L + GT+ L+
Sbjct: 710 EYHLRFGLKLMLLWDCVSPLSDDGTLVLA 738
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,898,972
Number of Sequences: 5004
Number of extensions: 55442
Number of successful extensions: 175
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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