BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP14_F_N22
(926 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.014
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 0.11
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 1.2
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 29 1.2
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 28 1.6
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 23 2.7
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 27 3.8
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 5.0
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 8.7
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 35.1 bits (77), Expect = 0.014
Identities = 18/55 (32%), Positives = 22/55 (40%), Gaps = 2/55 (3%)
Frame = +2
Query: 767 PPPXAPP--RXQXPPWXXKXXARGNXPHAXPGPASXXPXXXXHPPXXXTPPPPPP 925
PPP PP R + P + + P P P S PP + PPPPP
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPP 365
Score = 29.1 bits (62), Expect = 0.93
Identities = 24/108 (22%), Positives = 27/108 (25%), Gaps = 3/108 (2%)
Frame = +2
Query: 611 PXPPPPXXXXNPNXDPPP---SKXVXNXXXXXXXXXXXXXXXXXXXXXXXXPXXXPPPXA 781
P PPPP + PPP S+ V N P P
Sbjct: 361 PPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTP 420
Query: 782 PPRXQXPPWXXKXXARGNXPHAXPGPASXXPXXXXHPPXXXTPPPPPP 925
P P A + P P P PP P PP
Sbjct: 421 PSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPP 468
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 26.6 bits (56), Expect(2) = 0.11
Identities = 13/33 (39%), Positives = 15/33 (45%)
Frame = -3
Query: 663 GGGSXLGFXXXXGGGGXGLXGRVXGGGGXPXGW 565
G G G GGG G G + G GG P G+
Sbjct: 229 GPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGF 261
Score = 25.8 bits (54), Expect = 8.7
Identities = 16/34 (47%), Positives = 16/34 (47%), Gaps = 2/34 (5%)
Frame = -3
Query: 660 GGSXLGFXXXXGG--GGXGLXGRVXGGGGXPXGW 565
GG GF GG GG G G GG G P GW
Sbjct: 241 GGGPGGFGGGLGGFGGGPGGFGGGPGGHGGP-GW 273
Score = 24.2 bits (50), Expect(2) = 0.11
Identities = 17/52 (32%), Positives = 18/52 (34%)
Frame = -3
Query: 924 GGGGGGVXXXGGWXXXXGXXXAGPGWAXGXXPRAXXFXXQGGXCXRGGAXGG 769
G GGG GG+ G GPG G GG GG GG
Sbjct: 184 GHNGGGF---GGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGG 232
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/55 (32%), Positives = 20/55 (36%)
Frame = +2
Query: 755 PXXXPPPXAPPRXQXPPWXXKXXARGNXPHAXPGPASXXPXXXXHPPXXXTPPPP 919
P P P P PP R + P P PAS P PP + PPP
Sbjct: 124 PPSAPAPPTPQSELRPP--TSAPPRPSIP--PPSPASAPPIPSKAPPIPSSLPPP 174
Score = 25.8 bits (54), Expect = 8.7
Identities = 21/84 (25%), Positives = 24/84 (28%), Gaps = 5/84 (5%)
Frame = +2
Query: 428 PXXGXPPPXXGTGXAGXXGPTQXXIPHPXDGGXPGXPKKAXXXXA-----XQPCGXXXXX 592
P PP P + IP P P P KA + QP
Sbjct: 125 PSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSP 184
Query: 593 XTRPXXPXPPPPXXXXNPNXDPPP 664
+ P P PP P PPP
Sbjct: 185 PSAPSLPSAVPPMP---PKVPPPP 205
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 28.7 bits (61), Expect = 1.2
Identities = 19/52 (36%), Positives = 21/52 (40%)
Frame = -3
Query: 924 GGGGGGVXXXGGWXXXXGXXXAGPGWAXGXXPRAXXFXXQGGXCXRGGAXGG 769
GG GG GG+ G G G A G +GG RGGA GG
Sbjct: 9 GGRGGSRGGRGGFNGGRGGFGGGRGGARG----GGRGGARGGRGGRGGARGG 56
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.3 bits (60), Expect = 1.6
Identities = 10/21 (47%), Positives = 10/21 (47%)
Frame = +2
Query: 602 PXXPXPPPPXXXXNPNXDPPP 664
P P PPPP P PPP
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPP 26
Score = 26.2 bits (55), Expect = 6.6
Identities = 9/18 (50%), Positives = 10/18 (55%)
Frame = +2
Query: 611 PXPPPPXXXXNPNXDPPP 664
P PPPP P+ PPP
Sbjct: 10 PPPPPPPGFEPPSQPPPP 27
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 23.4 bits (48), Expect(2) = 2.7
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = +2
Query: 767 PPPXAPPRXQXPP 805
PPP APP PP
Sbjct: 1716 PPPSAPPMPAGPP 1728
Score = 22.2 bits (45), Expect(2) = 2.7
Identities = 8/18 (44%), Positives = 8/18 (44%)
Frame = +2
Query: 611 PXPPPPXXXXNPNXDPPP 664
P PPPP P PP
Sbjct: 1705 PTPPPPPMSVPPPPSAPP 1722
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 27.1 bits (57), Expect = 3.8
Identities = 12/25 (48%), Positives = 13/25 (52%)
Frame = +2
Query: 851 PGPASXXPXXXXHPPXXXTPPPPPP 925
P A+ P HP TPPPPPP
Sbjct: 892 PNDATSLPTIITHP----TPPPPPP 912
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 26.6 bits (56), Expect = 5.0
Identities = 18/54 (33%), Positives = 18/54 (33%), Gaps = 2/54 (3%)
Frame = +2
Query: 770 PPXAPPRXQXPPWXXKXXARGNXPHAXPGPASXXPXXXXHPPXXXT--PPPPPP 925
PP PP P A P P P P PP PPPPPP
Sbjct: 732 PPPPPPAVIVPT-----PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 25.8 bits (54), Expect = 8.7
Identities = 16/53 (30%), Positives = 17/53 (32%)
Frame = +1
Query: 766 PPPXGPPPXTXXPLXTKXXRPRXXPXRPXGARXXXXPXXXPPXRXXDPPPPXT 924
P P PPP T P T + P R PP PP P T
Sbjct: 236 PAPPPPPPPTLPPQSTNTSQ-LPMPSRNVNNLGSQVNIPPPPATPSQPPRPPT 287
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,690,759
Number of Sequences: 5004
Number of extensions: 18414
Number of successful extensions: 167
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 40
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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