Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP14_F_N20
         (852 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF015267-1|AAC38959.1|  461|Apis mellifera elongation factor-1al...   401   e-114
X52884-1|CAA37066.1|  461|Apis mellifera elongation factor 1 alp...   383   e-108
AY208278-1|AAO48970.1|  274|Apis mellifera elongation factor 1-a...   330   1e-92
EF013389-1|ABK54743.1|  172|Apis mellifera elongation factor 1-a...   298   4e-83
AF069739-1|AAC63272.2|  690|Apis mellifera translation initiatio...    35   8e-04
AM050259-1|CAJ18340.1|  683|Apis mellifera putative H3K9 methylt...    24   1.5  
AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase pro...    23   3.6  
D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.    23   4.7  

>AF015267-1|AAC38959.1|  461|Apis mellifera elongation factor-1alpha
           F2 protein.
          Length = 461

 Score =  401 bits (987), Expect = e-114
 Identities = 191/209 (91%), Positives = 195/209 (93%)
 Frame = +3

Query: 180 GHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLQAERERGITIDIALWKFETSK 359
           GHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL+AERERGITIDIALWKFETSK
Sbjct: 25  GHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSK 84

Query: 360 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 539
           YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG
Sbjct: 85  YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 144

Query: 540 VKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIXKIGYXPAAVAFVPISGWHGDXMLEP 719
           VKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYI KIGY PAAVAFVPISGWHGD MLE 
Sbjct: 145 VKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEV 204

Query: 720 STKMPWFKGWXGGXVRXAKLTGKCLIEXL 806
           S+KMPWFKGW     +  K+ GKCLIE L
Sbjct: 205 SSKMPWFKGWTVER-KEGKVEGKCLIEAL 232



 Score = 54.8 bits (126), Expect = 1e-09
 Identities = 25/27 (92%), Positives = 25/27 (92%)
 Frame = +2

Query: 107 MGKEKTHINIVVIGHVDSGKSTTTGSL 187
           MGKEK HINIVVIGHVDSGKSTTTG L
Sbjct: 1   MGKEKIHINIVVIGHVDSGKSTTTGHL 27


>X52884-1|CAA37066.1|  461|Apis mellifera elongation factor 1 alpha
           protein.
          Length = 461

 Score =  383 bits (942), Expect = e-108
 Identities = 186/226 (82%), Positives = 193/226 (85%), Gaps = 2/226 (0%)
 Frame = +3

Query: 180 GHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLQAERERGITIDIALWKFETSK 359
           GHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL+AERERGITIDIALWKFET+K
Sbjct: 25  GHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAK 84

Query: 360 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 539
           YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISKNGQTREHALLAFTLG
Sbjct: 85  YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLG 144

Query: 540 VKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIXKIGYXPAAVAFVPISGWHGDXMLEP 719
           VKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYI KIGY  A+VAFVPISGWHGD MLEP
Sbjct: 145 VKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEP 204

Query: 720 STKMPWFKGWXGGXVRXAKLTGKCLIEXLXC--HPGQPGPXPLTKP 851
           S K PW+KGW     +     GK LIE L     P +P    L  P
Sbjct: 205 SPKTPWYKGWKVER-KDGNADGKTLIEALDAILPPSRPTDKALRLP 249



 Score = 54.8 bits (126), Expect = 1e-09
 Identities = 25/27 (92%), Positives = 25/27 (92%)
 Frame = +2

Query: 107 MGKEKTHINIVVIGHVDSGKSTTTGSL 187
           MGKEK HINIVVIGHVDSGKSTTTG L
Sbjct: 1   MGKEKIHINIVVIGHVDSGKSTTTGHL 27


>AY208278-1|AAO48970.1|  274|Apis mellifera elongation factor
           1-alpha protein.
          Length = 274

 Score =  330 bits (810), Expect = 1e-92
 Identities = 158/176 (89%), Positives = 162/176 (92%)
 Frame = +3

Query: 279 WVLDKLQAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 458
           WVLDKL+AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1   WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60

Query: 459 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 638
           AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY
Sbjct: 61  AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120

Query: 639 IXKIGYXPAAVAFVPISGWHGDXMLEPSTKMPWFKGWXGGXVRXAKLTGKCLIEXL 806
           I KIGY PAAVAFVPISGWHGD MLE S+KMPWFKGW     +  K+ GKCLIE L
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVER-KEGKVEGKCLIEAL 175


>EF013389-1|ABK54743.1|  172|Apis mellifera elongation factor
           1-alpha protein.
          Length = 172

 Score =  298 bits (732), Expect = 4e-83
 Identities = 143/160 (89%), Positives = 146/160 (91%)
 Frame = +3

Query: 327 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 506
           DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1   DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60

Query: 507 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIXKIGYXPAAVAFVPI 686
           REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYI KIGY PAAVAFVPI
Sbjct: 61  REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120

Query: 687 SGWHGDXMLEPSTKMPWFKGWXGGXVRXAKLTGKCLIEXL 806
           SGWHGD MLE S+KMPWFKGW     +  K+ GKCLIE L
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVER-KEGKVEGKCLIEAL 159


>AF069739-1|AAC63272.2|  690|Apis mellifera translation initiation
           factor 2 protein.
          Length = 690

 Score = 35.1 bits (77), Expect = 8e-04
 Identities = 28/85 (32%), Positives = 38/85 (44%)
 Frame = +3

Query: 366 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 545
           VT +D PGH  FI     G    D  VL+VAA  G  E       QT +   +A    V 
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246

Query: 546 QLIVGVNKMDSTEPPYSEPRFEEIK 620
            +IV +NK+D       + ++E  K
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAK 271



 Score = 26.2 bits (55), Expect = 0.38
 Identities = 10/23 (43%), Positives = 15/23 (65%)
 Frame = +2

Query: 119 KTHINIVVIGHVDSGKSTTTGSL 187
           K H  + ++GHVD GK+T   +L
Sbjct: 143 KRHPIVTIMGHVDHGKTTLLDAL 165


>AM050259-1|CAJ18340.1|  683|Apis mellifera putative H3K9
           methyltransferase protein.
          Length = 683

 Score = 24.2 bits (50), Expect = 1.5
 Identities = 11/15 (73%), Positives = 11/15 (73%)
 Frame = +2

Query: 128 INIVVIGHVDSGKST 172
           INI  IGHV  GKST
Sbjct: 43  INIGTIGHVAHGKST 57


>AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase
           protein.
          Length = 567

 Score = 23.0 bits (47), Expect = 3.6
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = +3

Query: 351 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 452
           T KYY    D P +  FIKN+   ++ +D   LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327


>D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.
          Length = 567

 Score = 22.6 bits (46), Expect = 4.7
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = +3

Query: 351 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 452
           T KYY    D P +  FIKN+   ++ +D   L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 229,628
Number of Sequences: 438
Number of extensions: 4626
Number of successful extensions: 22
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 27431202
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)