Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP14_F_M02
(865 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 227 1e-60
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 219 5e-58
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 119 5e-28
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 104 2e-23
SPAPB1A11.01 ||SPAPB24D3.11|membrane transporter|Schizosaccharom... 29 1.1
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.9
SPBC1718.05 |trs31||TRAPP complex subunit Trs31 |Schizosaccharom... 26 7.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.9
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 227 bits (556), Expect = 1e-60
Identities = 97/133 (72%), Positives = 114/133 (85%)
Frame = +2
Query: 167 HVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVF 346
HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+VPR+++
Sbjct: 8 HVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIY 67
Query: 347 VDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLAD 526
VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD
Sbjct: 68 VDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127
Query: 527 QCTGLQGFLIFHS 565
C+GLQGFL+FHS
Sbjct: 128 NCSGLQGFLVFHS 140
Score = 33.9 bits (74), Expect = 0.030
Identities = 13/24 (54%), Positives = 20/24 (83%)
Frame = +3
Query: 600 LLMEXLSVDYGXKSKLEXAIYPAP 671
LL+E L+++Y KSKL+ ++YPAP
Sbjct: 152 LLLERLAMEYTKKSKLQFSVYPAP 175
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 219 bits (534), Expect = 5e-58
Identities = 97/138 (70%), Positives = 116/138 (84%), Gaps = 5/138 (3%)
Frame = +2
Query: 167 HVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHV 331
HVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSETG GK V
Sbjct: 8 HVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFV 66
Query: 332 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 511
PR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RI
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 512 RKLADQCTGLQGFLIFHS 565
R++AD C+GLQGFL+FHS
Sbjct: 127 RRMADNCSGLQGFLVFHS 144
Score = 35.1 bits (77), Expect = 0.013
Identities = 13/24 (54%), Positives = 20/24 (83%)
Frame = +3
Query: 600 LLMEXLSVDYGXKSKLEXAIYPAP 671
LL+E L+++YG KS L+ ++YPAP
Sbjct: 156 LLLERLNMEYGKKSNLQFSVYPAP 179
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 119 bits (287), Expect = 5e-28
Identities = 58/132 (43%), Positives = 78/132 (59%)
Frame = +2
Query: 173 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 352
GQ G Q+G A W EHG+ G T + N +F+E GK+VPRAV VD
Sbjct: 10 GQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVD 67
Query: 353 LEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQC 532
LEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 533 TGLQGFLIFHSL 568
LQGF + HSL
Sbjct: 128 DALQGFQLTHSL 139
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 104 bits (249), Expect = 2e-23
Identities = 63/183 (34%), Positives = 100/183 (54%), Gaps = 3/183 (1%)
Frame = +2
Query: 173 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 352
GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ +++PRA+ +D
Sbjct: 11 GQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILID 68
Query: 353 LEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLAD 526
LEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D I + AD
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127
Query: 527 QCTGLQGFLIFHSLRWRYRLWV-HFLIDGASLR*LRXEV*TGVRHLPRXSRFPLAVVEPY 703
L+GF + HS+ + FL++ + R + + T P VV+PY
Sbjct: 128 GSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQT-YSVFPNSQSVSDVVVQPY 186
Query: 704 NSI 712
NS+
Sbjct: 187 NSL 189
>SPAPB1A11.01 ||SPAPB24D3.11|membrane
transporter|Schizosaccharomyces pombe|chr 1|||Manual
Length = 495
Score = 28.7 bits (61), Expect = 1.1
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +2
Query: 545 GFLIFHSLRWRYRLWVHFLIDGASL 619
GF+ S+ WR+ W+ ++ G SL
Sbjct: 185 GFIAGSSISWRWEFWILLMLSGVSL 209
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.9
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 225 STASSLMARCPQTRPSGVETILSTLSSARPELAST 329
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPBC1718.05 |trs31||TRAPP complex subunit Trs31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 209
Score = 25.8 bits (54), Expect = 7.9
Identities = 16/59 (27%), Positives = 30/59 (50%), Gaps = 1/59 (1%)
Frame = +2
Query: 422 ITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIF-HSLRWRYRLWVH 595
++G ++ Y +G+++V+LV+ R R + T + G L + HS W+Y H
Sbjct: 61 VSGIQEFEEKLNEHGYRVGQKLVELVVWRERNPKRE-TRILGILQYIHSSVWKYLFGKH 118
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.9
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 244 WPDAHRQDHRGWRRFFQHFLQR 309
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,268,021
Number of Sequences: 5004
Number of extensions: 63519
Number of successful extensions: 176
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 430470850
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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