Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP14_F_L08
         (859 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC613.05c |rpl35||60S ribosomal protein L35|Schizosaccharomyce...    60   4e-10
SPAC3H8.06 |aur1||inositol phosphorylceramide synthase |Schizosa...    27   4.5  
SPBC1348.07 |||S. pombe specific DUF999 protein family 6|Schizos...    26   6.0  
SPAC977.06 |||S. pombe specific DUF999 family protein 3|Schizosa...    26   7.9  
SPBPB2B2.07c |||S. pombe specific DUF999 protein family 7|Schizo...    26   7.9  
SPBPB2B2.14c |||S. pombe specific DUF999 protein family 8|Schizo...    26   7.9  
SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor Raf1|S...    26   7.9  

>SPCC613.05c |rpl35||60S ribosomal protein L35|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 122

 Score = 60.1 bits (139), Expect = 4e-10
 Identities = 31/84 (36%), Positives = 45/84 (53%)
 Frame = +3

Query: 99  VKCSELRTKDXXXXXXXXXXXXXXXTNLRVAKVTGGVASKLSKIRVVRKAIARVYIVYHQ 278
           +K  ELR +                 +LRV K+ GG  SKLSKI+  RK IAR+  V ++
Sbjct: 3   LKTFELRKQSQENLAEQLQELRQELASLRVQKIAGGSGSKLSKIKTTRKDIARILTVINE 62

Query: 279 KMKVNLRNHYKNKKYKPLEFKSQE 350
             ++  R  YKNKKY PL+ + ++
Sbjct: 63  SNRLAAREAYKNKKYIPLDLRQKK 86



 Score = 50.4 bits (115), Expect = 3e-07
 Identities = 24/42 (57%), Positives = 32/42 (76%)
 Frame = +1

Query: 334 NLRAKKTRAMRKALTKHEAKIKTRKEIRKKSLFPPRVYAVKA 459
           +LR KKTRA+R+ALT +E   KT K+I+K+  FP R YA+KA
Sbjct: 81  DLRQKKTRAIRRALTPYEQSRKTLKQIKKERYFPLRKYALKA 122


>SPAC3H8.06 |aur1||inositol phosphorylceramide synthase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 422

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 11/32 (34%), Positives = 18/32 (56%), Gaps = 3/32 (9%)
 Frame = -2

Query: 471 IIYSSFNGIDSRW---EERFLSDLFPRLDLCF 385
           +++ +F  + + W   E  FLS +FPR   CF
Sbjct: 246 VVFGAFPSLHAGWAMLEALFLSHVFPRYRFCF 277


>SPBC1348.07 |||S. pombe specific DUF999 protein family
           6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 230

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 21/61 (34%), Positives = 25/61 (40%), Gaps = 2/61 (3%)
 Frame = +3

Query: 216 KLSKIRVVRKAIARVYIVYHQKMKVNLRNHYKNKKYKPL--EFKSQEDPCYAQGSY*TRS 389
           +L K   V K      IV  QK K+N++          L  EF    D CY  G   TRS
Sbjct: 15  ELCKPEKVNKQNLFTNIVKPQKDKINIKTDKIKFFLNNLFTEFSKFHDSCYPDGRISTRS 74

Query: 390 K 392
           K
Sbjct: 75  K 75


>SPAC977.06 |||S. pombe specific DUF999 family protein
           3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 189

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 20/61 (32%), Positives = 25/61 (40%), Gaps = 2/61 (3%)
 Frame = +3

Query: 216 KLSKIRVVRKAIARVYIVYHQKMKVNLRNHYKNKKYKPL--EFKSQEDPCYAQGSY*TRS 389
           +L K   V K      I+  QK K+N++          L  EF    D CY  G   TRS
Sbjct: 15  ELCKPEKVNKQNLFTNIIKPQKDKINIKTDKIKFFLNNLFTEFSKFHDSCYPDGRISTRS 74

Query: 390 K 392
           K
Sbjct: 75  K 75


>SPBPB2B2.07c |||S. pombe specific DUF999 protein family
           7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 165

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 20/61 (32%), Positives = 25/61 (40%), Gaps = 2/61 (3%)
 Frame = +3

Query: 216 KLSKIRVVRKAIARVYIVYHQKMKVNLRNHYKNKKYKPL--EFKSQEDPCYAQGSY*TRS 389
           +L K   V K      I+  QK K+N++          L  EF    D CY  G   TRS
Sbjct: 35  ELCKPEKVNKQNLFTNIIKPQKDKINIKTDKIKFFLNNLFTEFSKFHDSCYPDGRISTRS 94

Query: 390 K 392
           K
Sbjct: 95  K 95


>SPBPB2B2.14c |||S. pombe specific DUF999 protein family
           8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 230

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 21/62 (33%), Positives = 27/62 (43%), Gaps = 3/62 (4%)
 Frame = +3

Query: 216 KLSKIRVVRKAIARVYIVYHQKMKVNLRNHYKNKKYKP---LEFKSQEDPCYAQGSY*TR 386
           +L K   V K      I+  QK K+N++   K K +      EF    D CY  G   TR
Sbjct: 15  ELCKPEKVNKQNLFTNIIKPQKDKINIKTD-KIKFFLDNLFTEFSKFHDSCYPDGRISTR 73

Query: 387 SK 392
           SK
Sbjct: 74  SK 75


>SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor
           Raf1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 638

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 9/35 (25%), Positives = 20/35 (57%)
 Frame = +3

Query: 270 YHQKMKVNLRNHYKNKKYKPLEFKSQEDPCYAQGS 374
           +H +   +L +HYK  +    E+ +  D C+++G+
Sbjct: 277 FHSETIQSLESHYKLNQVGEKEYSTISDLCFSKGN 311


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,055,448
Number of Sequences: 5004
Number of extensions: 28088
Number of successful extensions: 73
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 72
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 73
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)