BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP14_F_K13
(870 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 39 0.001
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 0.024
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 33 0.040
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 33 0.070
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.86
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.5
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 28 1.5
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 27 2.6
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 4.6
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 38.7 bits (86), Expect = 0.001
Identities = 33/141 (23%), Positives = 34/141 (24%), Gaps = 3/141 (2%)
Frame = +3
Query: 396 PPPPXXXXXGGGXXXXXXXFFXPPPPPXKKXXXX---PPPXXGXXFXXKKXXXPPPPPXF 566
PPPP G PPPPP + PPP P PPP
Sbjct: 339 PPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNP---PAPPPAI 395
Query: 567 FXXKTXXXKXXXXXSXPXHXXXXHXPSPXXXXPPPPPPXXXXXXPXXFFXXPXXPPXPPX 746
S PS PP PP P P PP P
Sbjct: 396 PGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPI 455
Query: 747 XXPXXXXXXPXXPXPXXXXXP 809
P P P P
Sbjct: 456 APPLPAGMPAAPPLPPAAPAP 476
Score = 32.7 bits (71), Expect = 0.070
Identities = 36/161 (22%), Positives = 39/161 (24%), Gaps = 3/161 (1%)
Frame = +2
Query: 395 PPPPXXGXXXGGXXXXXXXFFXPPPPPXXKXXXX---PPPXXXXXFXXKKXXXPPPPPXX 565
PPPP G PPPPP PPP + PP PP
Sbjct: 339 PPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSS----RAVSNPPAPPPA 394
Query: 566 FXXKNXQXXKXXXXFXXXXXXXXXXXLPPXXXPPPPPPPXXXXXXXXFFFXPPXXPXPPX 745
++ PP PP PP P P P
Sbjct: 395 IPGRSAPALPPLGN-------ASRTSTPPVPTPPSLPPSAP----------PSLPPSAPP 437
Query: 746 XXPXXXXXXXPXPXPXPXXXPXPXXXXXPXXPXXXXPXPPP 868
P P P P P P P PPP
Sbjct: 438 SLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPP 478
Score = 31.9 bits (69), Expect = 0.12
Identities = 25/121 (20%), Positives = 28/121 (23%)
Frame = +1
Query: 394 PPPPXXXXXXGGGXXXXXXXFXXPPPPXXKKXXXPPPXXXXXXXXKKKXXXPPPPXXFFX 573
PPPP G PPPP + + P PP
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPG 397
Query: 574 KKPTXXKXXXXXPXXXTXXXXTXPPPXXXXPPPPPPXXXXXXPXLFFXXPXXPPPPXXXP 753
+ T T P PP PP P P PP P
Sbjct: 398 RSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAP 457
Query: 754 P 756
P
Sbjct: 458 P 458
Score = 30.7 bits (66), Expect = 0.28
Identities = 13/33 (39%), Positives = 15/33 (45%)
Frame = +3
Query: 462 PPPPPXKKXXXXPPPXXGXXFXXKKXXXPPPPP 560
PPPPP ++ PP G PPPPP
Sbjct: 313 PPPPPSRRNRGKPP--IGNGSSNSSLPPPPPPP 343
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 29.5 bits (63), Expect = 0.65
Identities = 18/71 (25%), Positives = 19/71 (26%), Gaps = 6/71 (8%)
Frame = +1
Query: 547 PPPPXXFFXKKPTXXKXXXXXPXXXTXXXXTXPPP------XXXXPPPPPPXXXXXXPXL 708
PPPP P P PPP PPPPPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRY 792
Query: 709 FFXXPXXPPPP 741
+ P P P
Sbjct: 793 YAPAPQAEPEP 803
Score = 29.1 bits (62), Expect = 0.86
Identities = 14/49 (28%), Positives = 14/49 (28%)
Frame = +2
Query: 722 PXXPXPPXXXPXXXXXXXPXPXPXPXXXPXPXXXXXPXXPXXXXPXPPP 868
P P P P P P P P P P P PPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 28.3 bits (60), Expect = 1.5
Identities = 12/32 (37%), Positives = 12/32 (37%)
Frame = +3
Query: 462 PPPPPXKKXXXXPPPXXGXXFXXKKXXXPPPP 557
PPP P PPP G PPPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 27.9 bits (59), Expect(2) = 0.024
Identities = 12/33 (36%), Positives = 12/33 (36%)
Frame = +2
Query: 461 PPPPPXXKXXXXPPPXXXXXFXXKKXXXPPPPP 559
P PPP PPP PPPPP
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 27.1 bits (57), Expect = 3.5
Identities = 16/56 (28%), Positives = 17/56 (30%)
Frame = +1
Query: 574 KKPTXXKXXXXXPXXXTXXXXTXPPPXXXXPPPPPPXXXXXXPXLFFXXPXXPPPP 741
K P P PP PPPPP P + P PPPP
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPP----PPPGVAGAGPPPPPPP 781
Score = 27.1 bits (57), Expect = 3.5
Identities = 13/48 (27%), Positives = 13/48 (27%)
Frame = +3
Query: 642 PSPXXXXPPPPPPXXXXXXPXXFFXXPXXPPXPPXXXPXXXXXXPXXP 785
P P P P P P P PP PP P P
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 25.8 bits (54), Expect = 8.0
Identities = 11/32 (34%), Positives = 11/32 (34%)
Frame = +2
Query: 464 PPPPXXKXXXXPPPXXXXXFXXKKXXXPPPPP 559
PPP PPP PPPPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 25.0 bits (52), Expect(2) = 0.024
Identities = 9/26 (34%), Positives = 11/26 (42%)
Frame = +2
Query: 662 PPPPPPPXXXXXXXXFFFXPPXXPXP 739
PPPPPPP ++ P P
Sbjct: 776 PPPPPPPPAVSAGGSRYYAPAPQAEP 801
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 33.5 bits (73), Expect = 0.040
Identities = 16/33 (48%), Positives = 16/33 (48%)
Frame = +2
Query: 644 LPPXXXPPPPPPPXXXXXXXXFFFXPPXXPXPP 742
LPP PPPPPPP F PP P PP
Sbjct: 4 LPPGNPPPPPPPPG---------FEPPSQPPPP 27
Score = 27.1 bits (57), Expect = 3.5
Identities = 10/30 (33%), Positives = 13/30 (43%)
Frame = +2
Query: 461 PPPPPXXKXXXXPPPXXXXXFXXKKXXXPP 550
PPPPP + PPP + K+ P
Sbjct: 12 PPPPPGFEPPSQPPPPPPPGYVKKRKNKTP 41
Score = 25.8 bits (54), Expect = 8.0
Identities = 10/26 (38%), Positives = 10/26 (38%)
Frame = +2
Query: 719 PPXXPXPPXXXPXXXXXXXPXPXPXP 796
PP P PP P P P P P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/32 (40%), Positives = 13/32 (40%)
Frame = +3
Query: 648 PXXXXPPPPPPXXXXXXPXXFFXXPXXPPXPP 743
P PPPPPP F P PP PP
Sbjct: 5 PPGNPPPPPPPPG--------FEPPSQPPPPP 28
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 32.7 bits (71), Expect = 0.070
Identities = 24/99 (24%), Positives = 24/99 (24%), Gaps = 1/99 (1%)
Frame = +1
Query: 463 PPPPXXKKXXXP-PPXXXXXXXXKKKXXXPPPPXXFFXKKPTXXKXXXXXPXXXTXXXXT 639
PP P P P K PP P P P
Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPP 1199
Query: 640 XPPPXXXXPPPPPPXXXXXXPXLFFXXPXXPPPPXXXPP 756
P P PP PPP P P P P PP
Sbjct: 1200 VPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPP 1238
Score = 27.5 bits (58), Expect = 2.6
Identities = 27/136 (19%), Positives = 27/136 (19%)
Frame = +1
Query: 463 PPPPXXKKXXXPPPXXXXXXXXKKKXXXPPPPXXFFXKKPTXXKXXXXXPXXXTXXXXTX 642
PP P P PP P P P
Sbjct: 1064 PPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPV 1123
Query: 643 PPPXXXXPPPPPPXXXXXXPXLFFXXPXXPPPPXXXPPXXXXXXPXXXXPXXPXXXXXXX 822
P P PP P P P P P P P P P
Sbjct: 1124 PKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPP------VPKPSVAAPPVPAPSSG 1177
Query: 823 XPPXXPXXXXXXPXPP 870
PP P PP
Sbjct: 1178 IPPVPKPAAGVPPVPP 1193
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.1 bits (62), Expect = 0.86
Identities = 22/75 (29%), Positives = 22/75 (29%)
Frame = -2
Query: 866 GXGXXXXXXGXXGGXXXXXXXXGXXGXXXXGXXXXXXGGXXXGGGGXXGXXKKXXGXXXX 687
G G G G G G G GG G GG G G
Sbjct: 187 GGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGP----GGFGG 242
Query: 686 XXGGGGGGXXXXGGG 642
GG GGG GGG
Sbjct: 243 GPGGFGGGLGGFGGG 257
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 1.5
Identities = 16/52 (30%), Positives = 16/52 (30%)
Frame = +1
Query: 646 PPXXXXPPPPPPXXXXXXPXLFFXXPXXPPPPXXXPPXXXXXXPXXXXPXXP 801
PP P PPPP P P PP P P P P P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPP------PSAPPMPAGPPSAPPPPLPASSAPSVP 1744
Score = 28.3 bits (60), Expect = 1.5
Identities = 14/35 (40%), Positives = 14/35 (40%)
Frame = +1
Query: 637 TXPPPXXXXPPPPPPXXXXXXPXLFFXXPXXPPPP 741
T PPP PPPP P P PPPP
Sbjct: 1706 TPPPPPMSVPPPPSAPPMPAGP------PSAPPPP 1734
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 28.3 bits (60), Expect = 1.5
Identities = 20/67 (29%), Positives = 21/67 (31%)
Frame = -1
Query: 864 GGXGXXXXGXXGXXXXXGXGXXXGXGXGXGXXXXXXXGXXXGGXGXXGGXKKKXXXXXXX 685
GG G G G G G G G G G GG G GG +
Sbjct: 9 GGRGGSRGGRGGFNGGRG-----GFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGG 63
Query: 684 XGGGGGG 664
GG GG
Sbjct: 64 RGGAKGG 70
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 27.5 bits (58), Expect = 2.6
Identities = 13/46 (28%), Positives = 14/46 (30%)
Frame = +2
Query: 545 PPPPPXXFXXKNXQXXKXXXXFXXXXXXXXXXXLPPXXXPPPPPPP 682
PPPPP +P PPPPPPP
Sbjct: 908 PPPPPLPVKTSLNTFSHPDSVNIVANDTSVAGVMPAFPPPPPPPPP 953
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.6 bits (56), Expect = 4.6
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = +2
Query: 644 LPPXXXPPPPPPP 682
LP PPPPPPP
Sbjct: 183 LPSDYNPPPPPPP 195
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,037,965
Number of Sequences: 5004
Number of extensions: 38933
Number of successful extensions: 356
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 39
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 434475230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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