Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP14_F_J09
         (1042 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe...    39   0.001
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr...    38   0.002
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M...    33   0.066
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p...    33   0.066
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    28   1.9  
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1...    25   2.4  
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||...    27   3.3  
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    27   4.4  

>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 273

 Score = 38.7 bits (86), Expect = 0.001
 Identities = 29/83 (34%), Positives = 29/83 (34%), Gaps = 2/83 (2%)
 Frame = -2

Query: 513 GXXGGRXGGGXXGXXXXGARXXGXGRGXXGXPGGRGGXGWXXGXXP--PTPXPGGXGXAG 340
           G  GG  GG   G    G      G G     GG GG G   G     P    GG G  G
Sbjct: 191 GGFGGGSGGPPPGPGGFGGFGGFGGEGHHH--GGHGGFGGGPGGFEGGPGGFGGGPGGFG 248

Query: 339 GXXGGRGXGXXXXGGPRXGXRXP 271
           G  GG G G    GG   G   P
Sbjct: 249 GGLGGFGGGPGGFGGGPGGHGGP 271



 Score = 35.1 bits (77), Expect = 0.016
 Identities = 25/64 (39%), Positives = 25/64 (39%), Gaps = 4/64 (6%)
 Frame = -2

Query: 438 RGXXGXPGGRGGXGWXXGXXPPTPXP----GGXGXAGGXXGGRGXGXXXXGGPRXGXRXP 271
           RG  G  GG GG G   G  PP P      GG G  G   GG G      GG   G    
Sbjct: 183 RGHNG--GGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGF 240

Query: 270 XGGP 259
            GGP
Sbjct: 241 GGGP 244



 Score = 34.3 bits (75), Expect = 0.029
 Identities = 19/42 (45%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
 Frame = -2

Query: 513 GXXGGRXG-GGXXGXXXXGARXXGXGRGXXGX-PGGRGGXGW 394
           G  GG  G GG  G    G    G G G  G  PGG GG GW
Sbjct: 232 GFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGGPGW 273



 Score = 32.3 bits (70), Expect = 0.12
 Identities = 20/49 (40%), Positives = 20/49 (40%), Gaps = 2/49 (4%)
 Frame = -1

Query: 427 GXPGGXXGGGLGXGXXAPDPRXRGXGXRGGXXGRAGX--GXGVXGGAPG 287
           G  GG  GG  G G   P P   G G  GG  G      G G  GG PG
Sbjct: 184 GHNGGGFGG-FGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPG 231


>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 305

 Score = 37.9 bits (84), Expect = 0.002
 Identities = 29/80 (36%), Positives = 31/80 (38%), Gaps = 1/80 (1%)
 Frame = -2

Query: 513 GXXGGRXGG-GXXGXXXXGARXXGXGRGXXGXPGGRGGXGWXXGXXPPTPXPGGXGXAGG 337
           G  GGR G  G  G    G    G GRG     GGRGG              GG G  GG
Sbjct: 6   GSRGGRGGSRGGRGGFNGGRGGFGGGRG-GARGGGRGG------------ARGGRGGRGG 52

Query: 336 XXGGRGXGXXXXGGPRXGXR 277
             GGRG      GG + G +
Sbjct: 53  ARGGRGGSSGGRGGAKGGAK 72



 Score = 31.1 bits (67), Expect = 0.27
 Identities = 22/56 (39%), Positives = 22/56 (39%), Gaps = 1/56 (1%)
 Frame = -2

Query: 426 GXPGGRGGXGWXXGXXPPTPXPGGXGXA-GGXXGGRGXGXXXXGGPRXGXRXPXGG 262
           G  G RGG G   G        GG G A GG  GG   G    GG R G     GG
Sbjct: 10  GRGGSRGGRGGFNGGR--GGFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGG 63



 Score = 30.7 bits (66), Expect = 0.35
 Identities = 19/47 (40%), Positives = 19/47 (40%)
 Frame = -1

Query: 427 GXPGGXXGGGLGXGXXAPDPRXRGXGXRGGXXGRAGXGXGVXGGAPG 287
           G  GG  GG  G G         G G RGG  G  G   G  GGA G
Sbjct: 26  GGFGGGRGGARGGGRGGA---RGGRGGRGGARGGRGGSSGGRGGAKG 69



 Score = 30.7 bits (66), Expect = 0.35
 Identities = 19/45 (42%), Positives = 20/45 (44%)
 Frame = -1

Query: 427 GXPGGXXGGGLGXGXXAPDPRXRGXGXRGGXXGRAGXGXGVXGGA 293
           G  GG  GGG G    A   R    G RGG  G +G   G  GGA
Sbjct: 30  GGRGGARGGGRGG---ARGGRGGRGGARGGRGGSSGGRGGAKGGA 71



 Score = 27.9 bits (59), Expect = 2.5
 Identities = 15/32 (46%), Positives = 15/32 (46%), Gaps = 3/32 (9%)
 Frame = -2

Query: 363 PGGXGXAGGXXGGRG---XGXXXXGGPRXGXR 277
           PG  G  GG  GGRG    G    GG R G R
Sbjct: 5   PGSRGGRGGSRGGRGGFNGGRGGFGGGRGGAR 36


>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 500

 Score = 33.1 bits (72), Expect = 0.066
 Identities = 23/59 (38%), Positives = 23/59 (38%)
 Frame = -2

Query: 438 RGXXGXPGGRGGXGWXXGXXPPTPXPGGXGXAGGXXGGRGXGXXXXGGPRXGXRXPXGG 262
           R   G  GGRGG G   G        GG G  GG  G RG G    G P  G   P  G
Sbjct: 444 RTGGGSRGGRGGFGGRGGF-------GGRGGFGGGRG-RGRGGARSGNPNRGSVAPFSG 494


>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
           protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 194

 Score = 33.1 bits (72), Expect = 0.066
 Identities = 20/56 (35%), Positives = 22/56 (39%), Gaps = 2/56 (3%)
 Frame = -2

Query: 438 RGXXGXPGGRGGXGWXXGXXPPTPXPGGXGXAGGXXGGR--GXGXXXXGGPRXGXR 277
           +G    P GRGG G   G    +    G    GG  GG   G G    GG R G R
Sbjct: 128 KGARNGPAGRGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRGGFGGGSRGGSRGGFR 183


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 28.3 bits (60), Expect = 1.9
 Identities = 11/21 (52%), Positives = 12/21 (57%)
 Frame = +1

Query: 394 PAXPPXXPRAPXXPPPXSXAP 456
           PA PP  P AP  PP  + AP
Sbjct: 464 PAAPPLPPAAPAPPPAPAPAP 484


>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1841

 Score = 24.6 bits (51), Expect(2) = 2.4
 Identities = 8/12 (66%), Positives = 9/12 (75%)
 Frame = +1

Query: 952 PPXPPXPPPXLS 987
           PP PP PPP +S
Sbjct: 945 PPPPPPPPPLVS 956



 Score = 21.4 bits (43), Expect(2) = 2.4
 Identities = 7/12 (58%), Positives = 7/12 (58%)
 Frame = +1

Query: 943 GXXPPXPPXPPP 978
           G  P  PP PPP
Sbjct: 939 GVMPAFPPPPPP 950


>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1461

 Score = 27.5 bits (58), Expect = 3.3
 Identities = 14/37 (37%), Positives = 14/37 (37%)
 Frame = +1

Query: 349 PXAPGXGGRGXXTPXPAXPPXXPRAPXXPPPXSXAPG 459
           P AP  GG     P P      P  P  PPP   A G
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGG 789



 Score = 26.2 bits (55), Expect = 7.6
 Identities = 9/15 (60%), Positives = 10/15 (66%)
 Frame = +1

Query: 943 GXXPPXPPXPPPXLS 987
           G  PP PP PPP +S
Sbjct: 772 GAGPPPPPPPPPAVS 786


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 27.1 bits (57), Expect = 4.4
 Identities = 10/19 (52%), Positives = 10/19 (52%)
 Frame = +3

Query: 267  RXGXGPXPGAPPXTPXPXP 323
            R   GP P APP  P P P
Sbjct: 956  RKASGPRPAAPPSIPPPLP 974



 Score = 26.2 bits (55), Expect = 7.6
 Identities = 12/44 (27%), Positives = 13/44 (29%)
 Frame = +3

Query: 288  PGAPPXTPXPXPALPXXXXXXXXXXXXXXXAXXPXPSPPPXXPP 419
            P APP  P P   +P                  P P P    PP
Sbjct: 1060 PSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPP 1103


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.316    0.148    0.526 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,144,075
Number of Sequences: 5004
Number of extensions: 12201
Number of successful extensions: 117
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 93
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 545231778
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)