BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP14_F_I01
(801 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 34 0.020
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.25
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.33
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 30 0.33
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.58
SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 28 1.8
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 4.1
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 5.4
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 7.2
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 23 8.2
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 34.3 bits (75), Expect = 0.020
Identities = 32/142 (22%), Positives = 34/142 (23%), Gaps = 5/142 (3%)
Frame = +2
Query: 389 APKPXXPXEFXPXPGPPKXXPSPXXPXXGPXTPPP---XGGXXPXXXXXPXXXXXPXXGG 559
AP P P P P P P P PPP P P G
Sbjct: 1031 APPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSG 1090
Query: 560 DPXXGGPXXXXPXXPXXXXXLSXPXAPPPXXPAXXXPPXXXXP--XXPXXXXXXPPXXXX 733
P P P P ++ P P P P P P P
Sbjct: 1091 IPPVPKPSVAAP--PVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVA 1148
Query: 734 XPXXXXXRXXPXXPXPXPXXPP 799
P P P P PP
Sbjct: 1149 APPVPAPSGAPPVPKPSVAAPP 1170
Score = 33.1 bits (72), Expect = 0.047
Identities = 25/111 (22%), Positives = 26/111 (23%)
Frame = +2
Query: 389 APKPXXPXEFXPXPGPPKXXPSPXXPXXGPXTPPPXGGXXPXXXXXPXXXXXPXXGGDPX 568
AP P P P P P P P P P P P
Sbjct: 1130 APPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVP 1189
Query: 569 XGGPXXXXPXXPXXXXXLSXPXAPPPXXPAXXXPPXXXXPXXPXXXXXXPP 721
P P P + P PPP P P P PP
Sbjct: 1190 PVPPPSEAPPVPKPS--VGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPP 1238
Score = 28.3 bits (60), Expect = 1.3
Identities = 26/105 (24%), Positives = 28/105 (26%)
Frame = +1
Query: 385 NGPETPXPPXIXPPPXXPQXGPFPPXXXXGAXNPPPXGGGXXXXXXXPXXXXXPXXGGGP 564
+ P P P PP P P P GA + PP P G P
Sbjct: 1030 DAPPIPVP-STAPPVPIPTSTPPVPKSSSGAPSAPP-----PVPAPSSEIPSIPAPSGAP 1083
Query: 565 XXXGPXXXXPXXXPXXXXPLXXXGXPPXXPRXXXPPXPXXXXXPP 699
P P P P P P PP P PP
Sbjct: 1084 PVPAPSGIPPVPKPSVAAP------PVPKPSVAVPPVPAPSGAPP 1122
Score = 27.9 bits (59), Expect = 1.8
Identities = 26/108 (24%), Positives = 26/108 (24%), Gaps = 5/108 (4%)
Frame = +1
Query: 391 PETPXPPXIXPPPXXPQXGPFPPXXXXGAXN-PPPXGGGXXXXXXXPXXXXXPXXGGGPX 567
P P P PP P P P A P P G P P
Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPP 1199
Query: 568 XXGPXXXXPXXXP---XXXXPLXXXGXPP-XXPRXXXPPXPXXXXXPP 699
P P P P G PP P PP P P
Sbjct: 1200 VPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAP 1247
Score = 27.1 bits (57), Expect = 3.1
Identities = 20/93 (21%), Positives = 21/93 (22%)
Frame = +2
Query: 392 PKPXXPXEFXPXPGPPKXXPSPXXPXXGPXTPPPXGGXXPXXXXXPXXXXXPXXGGDPXX 571
P P P P P P P G PP P P P
Sbjct: 1160 PVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPV 1219
Query: 572 GGPXXXXPXXPXXXXXLSXPXAPPPXXPAXXXP 670
P P P AP P+ P
Sbjct: 1220 PTPSAGLPPVPVPTAKAPPVPAPSSEAPSVSTP 1252
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 30.7 bits (66), Expect = 0.25
Identities = 17/63 (26%), Positives = 19/63 (30%)
Frame = +1
Query: 373 EMXLNGPETPXPPXIXPPPXXPQXGPFPPXXXXGAXNPPPXGGGXXXXXXXPXXXXXPXX 552
++ L P P P I P P PP G PPP G P P
Sbjct: 726 KLLLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAV 785
Query: 553 GGG 561
G
Sbjct: 786 SAG 788
Score = 25.8 bits (54), Expect = 7.2
Identities = 18/58 (31%), Positives = 19/58 (32%)
Frame = +2
Query: 485 PPPXGGXXPXXXXXPXXXXXPXXGGDPXXGGPXXXXPXXPXXXXXLSXPXAPPPXXPA 658
PPP P P P P GGP P P + P PPP PA
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPA----PIMGGP---PPPPPPPGVAGAGPPPPPPPPPA 784
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.3 bits (65), Expect = 0.33
Identities = 15/56 (26%), Positives = 16/56 (28%)
Frame = +2
Query: 629 PXAPPPXXPAXXXPPXXXXPXXPXXXXXXPPXXXXXPXXXXXRXXPXXPXPXPXXP 796
P + PP P PP P P PP P P P P P
Sbjct: 1686 PVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
Score = 27.1 bits (57), Expect = 3.1
Identities = 12/33 (36%), Positives = 13/33 (39%)
Frame = +1
Query: 400 PXPPXIXPPPXXPQXGPFPPXXXXGAXNPPPXG 498
P PP P P P P PP A + P G
Sbjct: 1715 PPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPG 1747
Score = 26.2 bits (55), Expect = 5.4
Identities = 12/42 (28%), Positives = 13/42 (30%)
Frame = +1
Query: 619 PLXXXGXPPXXPRXXXPPXPXXXXXPPXXXXXPPXXXXXPPP 744
P PP P+ PP PP PP P P
Sbjct: 1683 PAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMP 1724
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/35 (37%), Positives = 13/35 (37%)
Frame = +2
Query: 389 APKPXXPXEFXPXPGPPKXXPSPXXPXXGPXTPPP 493
AP P P P PP P P P P P P
Sbjct: 1704 APTPPPPP--MSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 30.3 bits (65), Expect = 0.33
Identities = 15/34 (44%), Positives = 16/34 (47%)
Frame = -1
Query: 492 GGGVXGPXXGXXGEGXXLGGPGXGXNSXGXXGFG 391
GGG GP G G G G G G + G GFG
Sbjct: 194 GGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFG 227
Score = 28.3 bits (60), Expect = 1.3
Identities = 21/69 (30%), Positives = 21/69 (30%), Gaps = 1/69 (1%)
Frame = -1
Query: 594 GXXXXGPPXXGSPPXXGXXXXXGXXXXXGXXPPXGGGVXGPXXGXXG-EGXXLGGPGXGX 418
G G G PP G G G GG G G G G GGPG
Sbjct: 189 GFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFG 248
Query: 417 NSXGXXGFG 391
G G G
Sbjct: 249 GGLGGFGGG 257
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.5 bits (63), Expect = 0.58
Identities = 32/138 (23%), Positives = 33/138 (23%), Gaps = 1/138 (0%)
Frame = +1
Query: 388 GPETPXPPXIXPPPXXPQXGPFPPXXXXG-AXNPPPXGGGXXXXXXXPXXXXXPXXGGGP 564
G P PP PP P G PP A + PP P P G
Sbjct: 357 GRSAPPPP---PPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPAL---PPLGNAS 410
Query: 565 XXXGPXXXXPXXXPXXXXPLXXXGXPPXXPRXXXPPXPXXXXXPPXXXXXPPXXXXXPPP 744
P P P P PP P P PP PP P
Sbjct: 411 RTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPL----PPSAPIAPPLPAGMPAA 466
Query: 745 XXXXXXXXXXPPXXXXXP 798
PP P
Sbjct: 467 PPLPPAAPAPPPAPAPAP 484
Score = 27.5 bits (58), Expect = 2.3
Identities = 16/60 (26%), Positives = 17/60 (28%), Gaps = 2/60 (3%)
Frame = +1
Query: 406 PPXIXP--PPXXPQXGPFPPXXXXGAXNPPPXGGGXXXXXXXPXXXXXPXXGGGPXXXGP 579
PP + P PP P P P A PP G P P P P
Sbjct: 428 PPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAP 487
>SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex
subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1522
Score = 27.9 bits (59), Expect = 1.8
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +2
Query: 422 PXPGPPKXXPSPXXPXXGPXTPPP 493
P PG K SP P P PPP
Sbjct: 149 PRPGTVKSSASPFVPNQNPSAPPP 172
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 4.1
Identities = 14/51 (27%), Positives = 14/51 (27%)
Frame = +1
Query: 592 PXXXPXXXXPLXXXGXPPXXPRXXXPPXPXXXXXPPXXXXXPPXXXXXPPP 744
P P P P P P P PP PP PPP
Sbjct: 124 PPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPP 174
Score = 26.6 bits (56), Expect = 4.1
Identities = 28/101 (27%), Positives = 29/101 (28%), Gaps = 1/101 (0%)
Frame = +2
Query: 389 APKPXXPX-EFXPXPGPPKXXPSPXXPXXGPXTPPPXGGXXPXXXXXPXXXXXPXXGGDP 565
AP P P E P P P P P P + PP P P P
Sbjct: 127 APAPPTPQSELRPPTSAP---PRPSIPPPSPASAPPIPSKAP-----PIPSSLPPPA--- 175
Query: 566 XXGGPXXXXPXXPXXXXXLSXPXAPPPXXPAXXXPPXXXXP 688
P P P S P A PP P PP P
Sbjct: 176 QPAAPVKSPPSAP------SLPSAVPPMPPKVPPPPLSQAP 210
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.2 bits (55), Expect = 5.4
Identities = 12/30 (40%), Positives = 13/30 (43%)
Frame = +2
Query: 389 APKPXXPXEFXPXPGPPKXXPSPXXPXXGP 478
AP P P P GPP P+ P GP
Sbjct: 1881 APPPPPPMAL-PKAGPPSAAPTSALPPAGP 1909
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 25.8 bits (54), Expect = 7.2
Identities = 14/40 (35%), Positives = 14/40 (35%)
Frame = +1
Query: 385 NGPETPXPPXIXPPPXXPQXGPFPPXXXXGAXNPPPXGGG 504
N P P PP PP P P PP N P G
Sbjct: 8 NPPPPPPPPGFEPPSQPPP--PPPPGYVKKRKNKTPAQSG 45
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 22.6 bits (46), Expect(2) = 8.2
Identities = 8/20 (40%), Positives = 8/20 (40%)
Frame = +2
Query: 434 PPKXXPSPXXPXXGPXTPPP 493
PP P P P PPP
Sbjct: 224 PPTYTPKQADPLPAPPPPPP 243
Score = 21.0 bits (42), Expect(2) = 8.2
Identities = 7/17 (41%), Positives = 9/17 (52%)
Frame = +2
Query: 389 APKPXXPXEFXPXPGPP 439
AP P ++ P P PP
Sbjct: 178 APATSLPSDYNPPPPPP 194
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,779,899
Number of Sequences: 5004
Number of extensions: 23833
Number of successful extensions: 142
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 31
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 88
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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