BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP14_F_G20
(955 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 37 0.005
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 35 0.015
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 32 0.14
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 24 1.7
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 24 4.6
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 24 5.0
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 5.1
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 5.1
SPAC26A3.08 |smb1|smb|Sm snRNP core protein Smb1|Schizosaccharom... 27 5.1
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 26 6.8
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 6.8
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 36.7 bits (81), Expect = 0.005
Identities = 15/24 (62%), Positives = 15/24 (62%)
Frame = +3
Query: 873 PPPPPPPXGXXXXXAXXXPPPPPP 944
PPPPPPP G A PPPPPP
Sbjct: 761 PPPPPPPPG--VAGAGPPPPPPPP 782
Score = 35.5 bits (78), Expect = 0.011
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = -3
Query: 653 PPPPPPGXXGXXPPPP 606
PPPPPPG G PPPP
Sbjct: 763 PPPPPPGVAGAGPPPP 778
Score = 35.1 bits (77), Expect = 0.015
Identities = 14/30 (46%), Positives = 14/30 (46%)
Frame = -1
Query: 652 PPPPPXGXGXXXPPPXXXFFXFXXPPPPPP 563
PPP P G PPP PPPPPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 33.1 bits (72), Expect = 0.059
Identities = 15/30 (50%), Positives = 15/30 (50%)
Frame = -1
Query: 652 PPPPPXGXGXXXPPPXXXFFXFXXPPPPPP 563
PPPPP G PPP PPPPPP
Sbjct: 763 PPPPPPGVAGAGPPP---------PPPPPP 783
Score = 30.7 bits (66), Expect = 0.32
Identities = 15/41 (36%), Positives = 15/41 (36%)
Frame = +1
Query: 823 PXPXPKKXKXXXXXGXXPPPPXPXXGXXXXXRXXXPPPPPP 945
P P P G PPPP P PPPPPP
Sbjct: 746 PAPIPVPPPAPIMGGPPPPPPPPGVAGAG---PPPPPPPPP 783
Score = 30.3 bits (65), Expect = 0.42
Identities = 13/30 (43%), Positives = 13/30 (43%)
Frame = -3
Query: 653 PPPPPPGXXGXXPPPPXXFFXFXXXPPPXP 564
P PPP G PPPP PPP P
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779
Score = 27.9 bits (59), Expect(2) = 0.28
Identities = 14/38 (36%), Positives = 15/38 (39%), Gaps = 7/38 (18%)
Frame = -3
Query: 656 APPPPPPGXXGXXP-------PPPXXFFXFXXXPPPXP 564
+PPPPPP P PPP PPP P
Sbjct: 731 SPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768
Score = 27.9 bits (59), Expect = 2.2
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = -3
Query: 653 PPPPPPGXXGXXPPPPXXFFXFXXXPPPXP 564
PPP P PPPP PPP P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 27.1 bits (57), Expect = 3.9
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +3
Query: 876 PPPPPPXGXXXXXAXXXPPPPPP 944
PPPPPP A P PPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPP 754
Score = 26.6 bits (56), Expect = 5.1
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = -3
Query: 653 PPPPPPGXXGXXPPPPXXFFXFXXXPPPXP 564
PP P G PPPP PPP P
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 21.4 bits (43), Expect(2) = 0.28
Identities = 7/13 (53%), Positives = 7/13 (53%)
Frame = -3
Query: 476 PPPPPXXVXXPPP 438
PPPPP PP
Sbjct: 764 PPPPPGVAGAGPP 776
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 35.1 bits (77), Expect = 0.015
Identities = 14/27 (51%), Positives = 14/27 (51%)
Frame = +3
Query: 873 PPPPPPPXGXXXXXAXXXPPPPPPXKK 953
PPPPPPP G PPPP KK
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPPGYVKK 35
Score = 33.1 bits (72), Expect = 0.059
Identities = 14/27 (51%), Positives = 14/27 (51%)
Frame = +3
Query: 864 GXXPPPPPPPXGXXXXXAXXXPPPPPP 944
G PPPPPPP PPPPPP
Sbjct: 7 GNPPPPPPPP---GFEPPSQPPPPPPP 30
Score = 29.5 bits (63), Expect = 0.73
Identities = 12/27 (44%), Positives = 13/27 (48%)
Frame = +1
Query: 874 PPPPXPXXGXXXXXRXXXPPPPPPXKK 954
PPPP P G + PPPP KK
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPPGYVKK 35
Score = 29.5 bits (63), Expect = 0.73
Identities = 15/35 (42%), Positives = 16/35 (45%)
Frame = -1
Query: 652 PPPPPXGXGXXXPPPXXXFFXFXXPPPPPPXXKKK 548
PPPPP G PP PPPPP KK+
Sbjct: 11 PPPPPPGFEPPSQPP---------PPPPPGYVKKR 36
Score = 29.1 bits (62), Expect = 0.97
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -3
Query: 653 PPPPPPGXXGXXPPPP 606
PPPPPPG PPP
Sbjct: 11 PPPPPPGFEPPSQPPP 26
Score = 28.7 bits (61), Expect = 1.3
Identities = 13/31 (41%), Positives = 13/31 (41%)
Frame = -3
Query: 641 PPGXXGXXPPPPXXFFXFXXXPPPXPXXXKK 549
PPG PPPP PPP P KK
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPPGYVKK 35
Score = 28.7 bits (61), Expect = 1.3
Identities = 15/35 (42%), Positives = 15/35 (42%)
Frame = -1
Query: 652 PPPPPXGXGXXXPPPXXXFFXFXXPPPPPPXXKKK 548
PPPPP G P PPPPPP KK
Sbjct: 9 PPPPPPPPGFEPPSQP--------PPPPPPGYVKK 35
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.9 bits (69), Expect = 0.14
Identities = 13/30 (43%), Positives = 13/30 (43%)
Frame = -1
Query: 652 PPPPPXGXGXXXPPPXXXFFXFXXPPPPPP 563
PPPPP PP PPPPPP
Sbjct: 313 PPPPPSRRNRGKPPIGNGSSNSSLPPPPPP 342
Score = 30.7 bits (66), Expect = 0.32
Identities = 14/31 (45%), Positives = 15/31 (48%), Gaps = 7/31 (22%)
Frame = +3
Query: 873 PPPPP-------PPXGXXXXXAXXXPPPPPP 944
PPPPP PP G + PPPPPP
Sbjct: 313 PPPPPSRRNRGKPPIGNGSSNSSLPPPPPPP 343
Score = 28.7 bits (61), Expect = 1.3
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = -3
Query: 653 PPPPPPGXXGXXPPPPXXFFXFXXXPPPXP 564
PPPPPP PP PPP P
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPP 341
Score = 28.7 bits (61), Expect = 1.3
Identities = 13/30 (43%), Positives = 13/30 (43%), Gaps = 6/30 (20%)
Frame = +3
Query: 873 PPPPPPPXGXXXXX------AXXXPPPPPP 944
PPPPPPP PPPPPP
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPP 366
Score = 27.5 bits (58), Expect = 2.9
Identities = 13/32 (40%), Positives = 13/32 (40%), Gaps = 2/32 (6%)
Frame = -1
Query: 652 PPPPPXGXGXXX--PPPXXXFFXFXXPPPPPP 563
PPPPP PPP PP PPP
Sbjct: 362 PPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPP 393
Score = 26.6 bits (56), Expect = 5.1
Identities = 14/34 (41%), Positives = 14/34 (41%), Gaps = 3/34 (8%)
Frame = -3
Query: 656 APPPPPP---GXXGXXPPPPXXFFXFXXXPPPXP 564
APPPPPP G PPP P P P
Sbjct: 360 APPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPP 393
Score = 25.8 bits (54), Expect = 9.0
Identities = 14/32 (43%), Positives = 14/32 (43%), Gaps = 2/32 (6%)
Frame = -3
Query: 653 PPPPPP--GXXGXXPPPPXXFFXFXXXPPPXP 564
PPPPPP G P PP PPP P
Sbjct: 338 PPPPPPRSNAAGSIPLPPQG----RSAPPPPP 365
Score = 25.8 bits (54), Expect = 9.0
Identities = 14/32 (43%), Positives = 14/32 (43%), Gaps = 2/32 (6%)
Frame = -1
Query: 652 PPPPPXGX--GXXXPPPXXXFFXFXXPPPPPP 563
PPPPP G PP PPPPPP
Sbjct: 339 PPPPPRSNAAGSIPLPPQGR----SAPPPPPP 366
Score = 25.8 bits (54), Expect = 9.0
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = -3
Query: 650 PPPPPGXXGXXPPPPXXFFXFXXXPPP 570
P PP G PPPP PPP
Sbjct: 352 PLPPQGRSAPPPPPPRSAPSTGRQPPP 378
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 23.8 bits (49), Expect(2) = 1.7
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = +3
Query: 873 PPPPPPP 893
PPPPPPP
Sbjct: 189 PPPPPPP 195
Score = 22.6 bits (46), Expect(2) = 1.7
Identities = 9/23 (39%), Positives = 9/23 (39%)
Frame = +3
Query: 876 PPPPPPXGXXXXXAXXXPPPPPP 944
P PP PPPPPP
Sbjct: 221 PEIPPTYTPKQADPLPAPPPPPP 243
Score = 22.6 bits (46), Expect(2) = 7.8
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -3
Query: 656 APPPPPP 636
APPPPPP
Sbjct: 237 APPPPPP 243
Score = 21.4 bits (43), Expect(2) = 7.8
Identities = 7/14 (50%), Positives = 7/14 (50%)
Frame = -3
Query: 647 PPPPGXXGXXPPPP 606
PPPP P PP
Sbjct: 273 PPPPATPSQPPRPP 286
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 23.8 bits (49), Expect(2) = 4.6
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = +3
Query: 873 PPPPPPP 893
PPPPPPP
Sbjct: 945 PPPPPPP 951
Score = 21.0 bits (42), Expect(2) = 4.6
Identities = 6/6 (100%), Positives = 6/6 (100%)
Frame = +3
Query: 927 PPPPPP 944
PPPPPP
Sbjct: 948 PPPPPP 953
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 23.8 bits (49), Expect(2) = 5.0
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = +3
Query: 873 PPPPPPP 893
PPPPPPP
Sbjct: 370 PPPPPPP 376
Score = 21.0 bits (42), Expect(2) = 5.0
Identities = 6/6 (100%), Positives = 6/6 (100%)
Frame = +3
Query: 927 PPPPPP 944
PPPPPP
Sbjct: 372 PPPPPP 377
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 5.1
Identities = 14/33 (42%), Positives = 14/33 (42%), Gaps = 2/33 (6%)
Frame = -3
Query: 656 APPPPPPGXXGXXPP--PPXXFFXFXXXPPPXP 564
AP PPPP PP PP PPP P
Sbjct: 1704 APTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 26.6 bits (56), Expect = 5.1
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +3
Query: 879 PPPPPXGXXXXXAXXXPPPPPP 944
PPPPP A PPPP
Sbjct: 908 PPPPPTASMTASAPAIASPPPP 929
>SPAC26A3.08 |smb1|smb|Sm snRNP core protein
Smb1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 147
Score = 26.6 bits (56), Expect = 5.1
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -1
Query: 649 PPPPXGXGXXXPPP 608
PPPP G G PPP
Sbjct: 129 PPPPAGFGRGAPPP 142
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 26.2 bits (55), Expect = 6.8
Identities = 11/29 (37%), Positives = 11/29 (37%)
Frame = +3
Query: 864 GXXPPPPPPPXGXXXXXAXXXPPPPPPXK 950
G PPPPPPP P P K
Sbjct: 303 GGLPPPPPPPPPSNDFWKDSNEPAPADNK 331
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 26.2 bits (55), Expect = 6.8
Identities = 15/46 (32%), Positives = 15/46 (32%), Gaps = 1/46 (2%)
Frame = -3
Query: 698 GGXGXPXXKXXXXXAPP-PPPPGXXGXXPPPPXXFFXFXXXPPPXP 564
G P K APP P P PPPP PP P
Sbjct: 716 GAVNSPAIKPQVTPAPPTPAPTPAVKHHPPPPPVRSSISPSMPPAP 761
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,673,789
Number of Sequences: 5004
Number of extensions: 50122
Number of successful extensions: 560
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 64
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 309
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 487313384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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