BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP14_F_G16
(921 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 37 0.005
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 36 0.008
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.075
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 3.7
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 3.7
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 6.5
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 26 8.6
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 36.7 bits (81), Expect = 0.005
Identities = 27/91 (29%), Positives = 30/91 (32%)
Frame = +1
Query: 649 STRAPPNXXPXXPKRGXXXGXFFXKXPPPPPXKXXXKTPPPXXXGGGTPPXXKXKTPPXX 828
ST PP P R K PPPP PPP G PP +
Sbjct: 286 STSKPP--LPPPSSRVSAAALAANKKRPPPP-------PPPSRRNRGKPPIGNGSSNSSL 336
Query: 829 XPPKKPPXXXPXXXPXPRRXKXKXXPPXPPP 921
PP PP P + + PP PPP
Sbjct: 337 PPPPPPPRSNAAGS-IPLPPQGRSAPPPPPP 366
Score = 35.9 bits (79), Expect = 0.008
Identities = 26/92 (28%), Positives = 31/92 (33%), Gaps = 3/92 (3%)
Frame = +1
Query: 655 RAPPNXXPXXPKRGXXX---GXFFXKXPPPPPXKXXXKTPPPXXXGGGTPPXXKXKTPPX 825
R PP P RG G PPPPP PP G P + ++ P
Sbjct: 310 RPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPP-------PPRSNAAGSIPLPPQGRSAPP 362
Query: 826 XXPPKKPPXXXPXXXPXPRRXKXKXXPPXPPP 921
PP+ P P + PP PPP
Sbjct: 363 PPPPRSAPSTGRQPPPLSSS-RAVSNPPAPPP 393
Score = 32.3 bits (70), Expect = 0.100
Identities = 18/64 (28%), Positives = 21/64 (32%)
Frame = +1
Query: 727 PPPPPXKXXXKTPPPXXXGGGTPPXXKXKTPPXXXPPKKPPXXXPXXXPXPRRXKXKXXP 906
PP PP ++ P G + TPP PP PP P P P
Sbjct: 388 PPAPPPAIPGRSAPALPPLGNA---SRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAP 444
Query: 907 PXPP 918
PP
Sbjct: 445 AAPP 448
Score = 27.5 bits (58), Expect = 2.8
Identities = 11/35 (31%), Positives = 13/35 (37%)
Frame = +3
Query: 810 KNPPXXXPPXKTPXXXPPLXXXPPXXKXKTXPPPP 914
K PP PP + PP+ PPPP
Sbjct: 309 KRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPP 343
Score = 26.2 bits (55), Expect = 6.5
Identities = 21/91 (23%), Positives = 21/91 (23%), Gaps = 4/91 (4%)
Frame = +1
Query: 661 PPNXXPXXPKRGXXX----GXFFXKXPPPPPXKXXXKTPPPXXXGGGTPPXXKXKTPPXX 828
PP P P R G PP P P PP P
Sbjct: 388 PPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAP 447
Query: 829 XPPKKPPXXXPXXXPXPRRXKXKXXPPXPPP 921
P P P P P PPP
Sbjct: 448 PLPPSAPIAPPLPAGMPAAPPLPPAAPAPPP 478
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 35.9 bits (79), Expect = 0.008
Identities = 26/86 (30%), Positives = 28/86 (32%)
Frame = +1
Query: 664 PNXXPXXPKRGXXXGXFFXKXPPPPPXKXXXKTPPPXXXGGGTPPXXKXKTPPXXXPPKK 843
P+ P PK PP K PP G PP K P PP
Sbjct: 1136 PSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPK---PAAGVPPVP 1192
Query: 844 PPXXXPXXXPXPRRXKXKXXPPXPPP 921
PP P P P+ PP PPP
Sbjct: 1193 PPSEAP---PVPK--PSVGVPPVPPP 1213
Score = 34.3 bits (75), Expect = 0.025
Identities = 19/64 (29%), Positives = 19/64 (29%), Gaps = 1/64 (1%)
Frame = +1
Query: 733 PPPXKXXXKTPPPXXXGGGTP-PXXKXKTPPXXXPPKKPPXXXPXXXPXPRRXKXKXXPP 909
PP K PP G P P PP P PP P P PP
Sbjct: 1121 PPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPP 1180
Query: 910 XPPP 921
P P
Sbjct: 1181 VPKP 1184
Score = 30.7 bits (66), Expect = 0.30
Identities = 19/64 (29%), Positives = 19/64 (29%), Gaps = 1/64 (1%)
Frame = +1
Query: 733 PPPXKXXXKTPPPXXXGGGTP-PXXKXKTPPXXXPPKKPPXXXPXXXPXPRRXKXKXXPP 909
PP K PP G P P PP P PP P P PP
Sbjct: 1102 PPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPP-VPAPSGAPP 1160
Query: 910 XPPP 921
P P
Sbjct: 1161 VPKP 1164
Score = 30.3 bits (65), Expect = 0.40
Identities = 20/67 (29%), Positives = 20/67 (29%), Gaps = 2/67 (2%)
Frame = +1
Query: 727 PPPPPXKXXXKTPPPXXXGGGTPPXXKXKT--PPXXXPPKKPPXXXPXXXPXPRRXKXKX 900
P P P PPP PP K PP P PP P P
Sbjct: 1180 PVPKPAAGVPPVPPPSE----APPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAK 1235
Query: 901 XPPXPPP 921
PP P P
Sbjct: 1236 APPVPAP 1242
Score = 28.7 bits (61), Expect = 1.2
Identities = 19/64 (29%), Positives = 19/64 (29%), Gaps = 1/64 (1%)
Frame = +1
Query: 733 PPPXKXXXKTPPPXXXGGGTPPXXKXKT-PPXXXPPKKPPXXXPXXXPXPRRXKXKXXPP 909
PP K K PP PP T PP P PP P PP
Sbjct: 1012 PPVPKLSSKAPPVPLPSADAPPIPVPSTAPPVPIPTSTPP------VPKSSSGAPSAPPP 1065
Query: 910 XPPP 921
P P
Sbjct: 1066 VPAP 1069
Score = 28.7 bits (61), Expect = 1.2
Identities = 21/87 (24%), Positives = 22/87 (25%), Gaps = 1/87 (1%)
Frame = +1
Query: 664 PNXXPXXPKRGXXXGXFFXKXPPPPPXKXXXKTPPPXXXGGGTP-PXXKXKTPPXXXPPK 840
P+ P PK PP K PP G P P PP P
Sbjct: 1117 PSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSS 1176
Query: 841 KPPXXXPXXXPXPRRXKXKXXPPXPPP 921
P P PP P P
Sbjct: 1177 GIPPVPKPAAGVPPVPPPSEAPPVPKP 1203
Score = 28.7 bits (61), Expect = 1.2
Identities = 17/64 (26%), Positives = 18/64 (28%)
Frame = +1
Query: 730 PPPPXKXXXKTPPPXXXGGGTPPXXKXKTPPXXXPPKKPPXXXPXXXPXPRRXKXKXXPP 909
PP P P P G P + PP P P P P PP
Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPP 1228
Query: 910 XPPP 921
P P
Sbjct: 1229 VPVP 1232
Score = 27.1 bits (57), Expect = 3.7
Identities = 19/68 (27%), Positives = 20/68 (29%), Gaps = 6/68 (8%)
Frame = +1
Query: 736 PPXKXXXKTPPPXXXGGGTP------PXXKXKTPPXXXPPKKPPXXXPXXXPXPRRXKXK 897
PP TPP G P P + P P PP P P P
Sbjct: 1041 PPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIP-PVPKPSV 1099
Query: 898 XXPPXPPP 921
PP P P
Sbjct: 1100 AAPPVPKP 1107
Score = 26.2 bits (55), Expect = 6.5
Identities = 24/92 (26%), Positives = 25/92 (27%), Gaps = 1/92 (1%)
Frame = +1
Query: 649 STRAPPNXXPXXPKRGXXXGXFFXKXPPPPPXKXXXKTPPPXXXGGGTPPXXKXKT-PPX 825
S AP P PK PP T PP TPP K + P
Sbjct: 1003 SPAAPLARVPPVPKLSSKAPPVPLPSADAPPIPVP-STAPPVPIPTSTPPVPKSSSGAPS 1061
Query: 826 XXPPKKPPXXXPXXXPXPRRXKXKXXPPXPPP 921
PP P P P P PP
Sbjct: 1062 APPPVPAPSSEIPSIPAPSGAPPVPAPSGIPP 1093
Score = 26.2 bits (55), Expect = 6.5
Identities = 18/64 (28%), Positives = 18/64 (28%), Gaps = 2/64 (3%)
Frame = +1
Query: 736 PPXKXXXKTPPPXXXGGGTPPXXKXKT--PPXXXPPKKPPXXXPXXXPXPRRXKXKXXPP 909
PP PP PP K PP P PP P P PP
Sbjct: 1083 PPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPP-VPVPSGAPP 1141
Query: 910 XPPP 921
P P
Sbjct: 1142 VPKP 1145
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 32.7 bits (71), Expect = 0.075
Identities = 17/53 (32%), Positives = 17/53 (32%)
Frame = +1
Query: 721 KXPPPPPXKXXXKTPPPXXXGGGTPPXXKXKTPPXXXPPKKPPXXXPXXXPXP 879
K PPPPP TP P P PP PP P P P
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 31.9 bits (69), Expect = 0.13
Identities = 21/62 (33%), Positives = 22/62 (35%), Gaps = 3/62 (4%)
Frame = +1
Query: 745 KXXXKTPPPXXXGGGTPPXXKXKTP---PXXXPPKKPPXXXPXXXPXPRRXKXKXXPPXP 915
K K+PPP PP TP P PP P P P P PP P
Sbjct: 726 KLLLKSPPPP------PPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779
Query: 916 PP 921
PP
Sbjct: 780 PP 781
Score = 29.5 bits (63), Expect = 0.70
Identities = 13/37 (35%), Positives = 14/37 (37%)
Frame = +3
Query: 810 KNPPXXXPPXKTPXXXPPLXXXPPXXKXKTXPPPPXP 920
K+PP P P P PP PPPP P
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPP 766
Score = 25.8 bits (54), Expect = 8.6
Identities = 11/32 (34%), Positives = 11/32 (34%)
Frame = +1
Query: 727 PPPPPXKXXXKTPPPXXXGGGTPPXXKXKTPP 822
PPP P PPP G P PP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.1 bits (57), Expect = 3.7
Identities = 15/50 (30%), Positives = 15/50 (30%)
Frame = +1
Query: 766 PPXXXGGGTPPXXKXKTPPXXXPPKKPPXXXPXXXPXPRRXKXKXXPPXP 915
PP PP TPP PP P P P PP P
Sbjct: 1690 PPVRPQSAAPPQMSAPTPP---PPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 26.6 bits (56), Expect = 4.9
Identities = 14/47 (29%), Positives = 15/47 (31%), Gaps = 1/47 (2%)
Frame = +1
Query: 736 PPXKXXXKTPPPXXXGGGTPPXXKXKTPPXXXP-PKKPPXXXPXXXP 873
PP + PP PP PP P P PP P P
Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 26.6 bits (56), Expect = 4.9
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = +3
Query: 816 PPXXXPPXKTPXXXPPLXXXPPXXKXKTXPPPPXP 920
PP PP P PP+ PP + PPPP P
Sbjct: 1710 PPMSVPP---PPSAPPMPAGPP-----SAPPPPLP 1736
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.1 bits (57), Expect = 3.7
Identities = 10/25 (40%), Positives = 12/25 (48%)
Frame = +3
Query: 846 PXXXPPLXXXPPXXKXKTXPPPPXP 920
P PP PP + + PPPP P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPP 29
Score = 26.2 bits (55), Expect = 6.5
Identities = 12/31 (38%), Positives = 13/31 (41%)
Frame = +1
Query: 727 PPPPPXKXXXKTPPPXXXGGGTPPXXKXKTP 819
PPPPP + PP G K KTP
Sbjct: 11 PPPPPPGFEPPSQPPPPPPPGYVKKRKNKTP 41
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 6.5
Identities = 16/63 (25%), Positives = 17/63 (26%)
Frame = +1
Query: 730 PPPPXKXXXKTPPPXXXGGGTPPXXKXKTPPXXXPPKKPPXXXPXXXPXPRRXKXKXXPP 909
PP PPP P P PP +P P P PP
Sbjct: 139 PPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAA--PVKSPPSAPSLPSAVPP 196
Query: 910 XPP 918
PP
Sbjct: 197 MPP 199
Score = 25.8 bits (54), Expect = 8.6
Identities = 13/34 (38%), Positives = 14/34 (41%), Gaps = 2/34 (5%)
Frame = +3
Query: 816 PPXXXP--PXKTPXXXPPLXXXPPXXKXKTXPPP 911
PP P P K+P P L P K PPP
Sbjct: 172 PPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPP 205
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 25.8 bits (54), Expect = 8.6
Identities = 11/34 (32%), Positives = 14/34 (41%)
Frame = +1
Query: 721 KXPPPPPXKXXXKTPPPXXXGGGTPPXXKXKTPP 822
K P PPP + + P P PP + PP
Sbjct: 47 KKPLPPPTRRLPRKPLPFRSTSLQPPSSQPPAPP 80
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.315 0.147 0.528
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,513,050
Number of Sequences: 5004
Number of extensions: 18208
Number of successful extensions: 148
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 77
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 468512460
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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