Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP14_F_E16
(822 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 89 5e-19
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 84 2e-17
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 59 9e-10
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 47 3e-06
SPAC1F12.05 |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.6
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 89.4 bits (212), Expect = 5e-19
Identities = 40/59 (67%), Positives = 47/59 (79%)
Frame = +2
Query: 257 PQTRTSGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDXVRTGTYRQLFHPEQLITG 433
P+T S D F+TFFSETG GK+VPR+++VDLEP V+D VRTG YR LFHPEQLITG
Sbjct: 38 PET-ASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITG 95
Score = 77.4 bits (182), Expect = 2e-15
Identities = 35/53 (66%), Positives = 40/53 (75%), Gaps = 4/53 (7%)
Frame = +1
Query: 148 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQM----PTDKNIGGWRRFF 294
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + + GG+ FF
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFF 53
Score = 48.4 bits (110), Expect = 1e-06
Identities = 22/41 (53%), Positives = 28/41 (68%)
Frame = +3
Query: 408 FIQNNLLLVKEXAANNYARGHYTIGKEIVNLXLXKIRKPXD 530
F L+ KE A+NNYARGHYT+GKE+V+ KIR+ D
Sbjct: 87 FHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 84.2 bits (199), Expect = 2e-17
Identities = 34/50 (68%), Positives = 42/50 (84%)
Frame = +2
Query: 284 DDSFNTFFSETGAGKHVPRAVFVDLEPTVVDXVRTGTYRQLFHPEQLITG 433
+D F TFFSETG GK VPR+++VDLEP V+D VRTG Y+ LFHPEQ++TG
Sbjct: 50 NDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTG 99
Score = 77.0 bits (181), Expect = 3e-15
Identities = 34/41 (82%), Positives = 36/41 (87%)
Frame = +1
Query: 148 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKN 270
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ +
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENS 40
Score = 48.0 bits (109), Expect = 2e-06
Identities = 20/41 (48%), Positives = 29/41 (70%)
Frame = +3
Query: 408 FIQNNLLLVKEXAANNYARGHYTIGKEIVNLXLXKIRKPXD 530
F ++ KE A+NNYARGHYT+GKE+++ L +IR+ D
Sbjct: 91 FHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMAD 131
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 58.8 bits (136), Expect = 9e-10
Identities = 26/60 (43%), Positives = 36/60 (60%)
Frame = +2
Query: 269 TSGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDXVRTGTYRQLFHPEQLITG*GXCG 448
TS + N +F+E GK+VPRAV VDLEP +D V++G + LF P+ +I G G
Sbjct: 39 TSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAG 98
Score = 34.7 bits (76), Expect = 0.016
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +1
Query: 148 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDG 249
MRE + + GQ G Q+G A W EHG+ G
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAG 34
Score = 31.9 bits (69), Expect = 0.11
Identities = 13/38 (34%), Positives = 23/38 (60%)
Frame = +3
Query: 408 FIQNNLLLVKEXAANNYARGHYTIGKEIVNLXLXKIRK 521
F +N++ + A N +A+GHYT G E+ + L +R+
Sbjct: 85 FRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRR 122
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 47.2 bits (107), Expect = 3e-06
Identities = 18/49 (36%), Positives = 33/49 (67%)
Frame = +2
Query: 281 GDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDXVRTGTYRQLFHPEQLI 427
G D + FF ++ +++PRA+ +DLEP VV+ + + TY L++PE ++
Sbjct: 44 GVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENIL 92
Score = 46.8 bits (106), Expect = 4e-06
Identities = 19/35 (54%), Positives = 24/35 (68%)
Frame = +1
Query: 151 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQM 255
RE I++ GQ G QIG+ W+ CLEHGI PDG +
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTL 37
>SPAC1F12.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 377
Score = 26.2 bits (55), Expect = 5.6
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = -2
Query: 278 PPMFLSVGIWPSGWMPCSRQ*SSQQALPIWTPAWPT 171
PPMF SVG P + +R SS +P+ P+ PT
Sbjct: 339 PPMFNSVGPSPPAYEQVAR--SSPTDIPLPPPSCPT 372
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +2
Query: 137 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 259
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,788,705
Number of Sequences: 5004
Number of extensions: 52950
Number of successful extensions: 126
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 402440190
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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