Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP14_F_D21
(887 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr 3... 35 0.018
SPAC26A3.06 |||methyltransferase |Schizosaccharomyces pombe|chr ... 32 0.095
SPBC21C3.07c |||actin binding methyltransferase |Schizosaccharom... 31 0.17
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S... 31 0.29
SPBC1347.09 |||hexaprenyldihydroxybenzoate methyltransferase|Sch... 28 1.5
SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydro... 28 2.0
SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Sc... 27 4.7
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 26 8.2
>SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 260
Score = 34.7 bits (76), Expect = 0.018
Identities = 31/116 (26%), Positives = 50/116 (43%), Gaps = 1/116 (0%)
Frame = +1
Query: 127 YQKSNCYPRRDA-VDVLTEFLPKFKWKEGKKRIFDIGCADGSVTNIISAFCNNDYEVFEA 303
Y + Y R + V LT+ + K + D+GC DG +TN + + C + +
Sbjct: 5 YWSAKDYQRNASFVPKLTKDIVKRINLSSSDELLDLGCGDGVLTNELVSQCRRVVGIDAS 64
Query: 304 CDINEQAVKYATEYYGTDKMIFRVMDIEGQLPKEMNGKFDNVFSFYTLQWIKNQEK 471
D+ + A + Y +++D ++P E FD VFS L WI Q K
Sbjct: 65 PDMIKAARELGLNAYVIPGE--KLLD-ASEIPSE---SFDVVFSNAALHWIMRQPK 114
>SPAC26A3.06 |||methyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 268
Score = 32.3 bits (70), Expect = 0.095
Identities = 26/89 (29%), Positives = 37/89 (41%)
Frame = +1
Query: 205 EGKKRIFDIGCADGSVTNIISAFCNNDYEVFEACDINEQAVKYATEYYGTDKMIFRVMDI 384
EG I DIGC G T I + V DI+ + A E + + + D+
Sbjct: 46 EGPSFILDIGCGSGISTQI----GESQGHVVVGMDISPSMLSVALESQEIEGDLL-LCDM 100
Query: 385 EGQLPKEMNGKFDNVFSFYTLQWIKNQEK 471
+P G FD V S +QW+ N +K
Sbjct: 101 GTGVPFRP-GTFDGVISISAIQWLLNADK 128
>SPBC21C3.07c |||actin binding methyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 281
Score = 31.5 bits (68), Expect = 0.17
Identities = 33/142 (23%), Positives = 57/142 (40%), Gaps = 7/142 (4%)
Frame = +1
Query: 151 RRDAVDVLTEFLPKFKWKEGKKRIFDIGCADG-SVTNIISAFCNNDYEVFEACDINEQAV 327
RR E L K G+K I +IGC G ++ I+ N++ ++F A D +E+A+
Sbjct: 98 RRWIAQEFPELLDLLKEDAGEKSILEIGCGAGNTIWPILKENKNSNLKIF-AVDYSEKAI 156
Query: 328 KYATE--YYGTDKMIFRVMDIEGQ--LPKEMNGKFDNVFSFYTLQWIK--NQEKAFQNIY 489
+ Y V D+ G L D + + + ++A +N+Y
Sbjct: 157 DVVKQNPLYDAKFCSASVWDLAGSDLLRSIEEASIDAITLIFCFSALSPDQWQQAIENLY 216
Query: 490 DLMTKDGECLLTLLARIPVYSL 555
L+ G L R+ + L
Sbjct: 217 RLLKPGGLILFRDYGRLDLTQL 238
>SPBC336.05c |||S-adenosylmethionine-
dependentmethyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 378
Score = 30.7 bits (66), Expect = 0.29
Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = +1
Query: 214 KRIFDIGCADGSVTNIISAFCNNDY--EVFEACDINEQAVKYATE 342
+ + DIGC D + + CN+ E DINEQ+++ ATE
Sbjct: 29 RSLLDIGCGDARFLSYLVP-CNDQVPIEFLAGIDINEQSIERATE 72
>SPBC1347.09 |||hexaprenyldihydroxybenzoate
methyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 284
Score = 28.3 bits (60), Expect = 1.5
Identities = 21/117 (17%), Positives = 45/117 (38%), Gaps = 4/117 (3%)
Frame = +1
Query: 199 WKEGKKRIFDIGCADGSVTNIISAFCNNDYEVFEACDI----NEQAVKYATEYYGTDKMI 366
WK+ I D C G ++ + +C + + D+ NE+ K +
Sbjct: 74 WKKSGMSILDFACGTGLISQHLFPYCKQIVGIDVSQDMVDVYNEKFRKMNIPKERACAYV 133
Query: 367 FRVMDIEGQLPKEMNGKFDNVFSFYTLQWIKNQEKAFQNIYDLMTKDGECLLTLLAR 537
+ D++G + + +FD V IK+ ++ + L+ +G + L +
Sbjct: 134 LSLDDLDGNGDEPFSTEFDAVVCSMAYHHIKDLQEVTNKLSKLLKPNGRLFVADLIK 190
>SPAC23H4.10c |thi4||thiamine-phosphate
dipyrophosphorylase/hydroxyethylthiazole kinase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 518
Score = 27.9 bits (59), Expect = 2.0
Identities = 15/45 (33%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
Frame = +1
Query: 160 AVDVLTEFLPKFKWKEGKKRIFDI---GCADGSVTNIISAFCNND 285
AVDV+++ + K G + I GC+ GSV + ++ C ND
Sbjct: 405 AVDVISDGNSTYVIKNGNPLLGQITASGCSLGSVMGVTASICQND 449
>SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 785
Score = 26.6 bits (56), Expect = 4.7
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -3
Query: 495 QVVNVLKCFLLILDPLQRIERENVIKLPIH 406
Q+ ++K F DPL +IE+ V+ PIH
Sbjct: 520 QMSQLVKNFYAFEDPLLQIEKSLVLNKPIH 549
>SPAC23A1.04c |mnl1||alpha mannosidase-like
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 25.8 bits (54), Expect = 8.2
Identities = 24/65 (36%), Positives = 33/65 (50%)
Frame = +1
Query: 445 LQWIKNQEKAFQNIYDLMTKDGECLLTLLARIPVYSLFNALKDTDTWGYLLKDINQFISP 624
+Q KN KA + L+T DGE + L SLFN +K LLK+ N F+S
Sbjct: 697 VQQAKNTNKA--GLLILIT-DGEFINPLENIHSQNSLFNWVKPYIPPTILLKNENNFVSK 753
Query: 625 YHDVS 639
+ +VS
Sbjct: 754 WANVS 758
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,312,636
Number of Sequences: 5004
Number of extensions: 67840
Number of successful extensions: 200
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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