Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP14_F_D13
(816 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 43 6e-05
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 40 6e-04
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.048
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.4
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 3.2
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 4.2
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 27 4.2
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 26 7.4
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 25 9.7
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 42.7 bits (96), Expect = 6e-05
Identities = 28/77 (36%), Positives = 28/77 (36%), Gaps = 1/77 (1%)
Frame = -2
Query: 674 GXGGGGGXXXXPPPPPXXXXXXGGGGXXKXXGGGXXXXXXXXXXFWGG-GGXXXXPPXFF 498
G GG GG PPP P GG G GG F GG GG P F
Sbjct: 189 GFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFG 248
Query: 497 FXXGGGGGGXXXFFXPP 447
GG GGG F P
Sbjct: 249 GGLGGFGGGPGGFGGGP 265
Score = 27.9 bits (59), Expect = 1.8
Identities = 17/46 (36%), Positives = 17/46 (36%)
Frame = -3
Query: 610 GGGGXXKKXGGGGXXPPPXKKXXFGGGGGXXXXPXFFFXXXGGGGG 473
GGG G GG P P FGG GG GG GG
Sbjct: 187 GGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGG 232
Score = 27.5 bits (58), Expect = 2.4
Identities = 14/28 (50%), Positives = 14/28 (50%)
Frame = -1
Query: 681 FXGXGGGGGGXXXSPPPPXXGXXXGGGG 598
F G GGG GG PPP G G GG
Sbjct: 190 FGGFGGGSGG-----PPPGPGGFGGFGG 212
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 39.5 bits (88), Expect = 6e-04
Identities = 25/74 (33%), Positives = 25/74 (33%)
Frame = +2
Query: 473 PPPPPPXXKKKXGXXXXXPPPPKXXFFXXXXXXXXXXXFFXXPPPPXXXPXXGGGGXXXX 652
PPPPPP P PP PPPP P G G
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGG-------------PPPPPPPPGVAGAGP--- 775
Query: 653 PPPPPPXPKXXGGG 694
PPPPPP P GG
Sbjct: 776 PPPPPPPPAVSAGG 789
Score = 39.1 bits (87), Expect = 7e-04
Identities = 15/32 (46%), Positives = 15/32 (46%)
Frame = +3
Query: 573 PPPPXFXXXPPPPXXXPXGGGGGXXXXPPPPP 668
PPP PPPP P G G PPPPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 37.5 bits (83), Expect = 0.002
Identities = 25/68 (36%), Positives = 25/68 (36%)
Frame = +1
Query: 472 PPPPPPXXKKKXGGXXXXPPPPQXXFFFXGGGXXPPPXXFXXPPPPXXXXXXGGGGGXXX 651
PPPPPP P PP G PPP PPPP G G
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIM----GGPPPP-----PPPP-------GVAGAGP 775
Query: 652 XPPPPPXP 675
PPPPP P
Sbjct: 776 PPPPPPPP 783
Score = 31.1 bits (67), Expect = 0.20
Identities = 15/40 (37%), Positives = 15/40 (37%)
Frame = +1
Query: 421 GGXPXFXXGGGXKKXXXPPPPPPXXKKKXGGXXXXPPPPQ 540
GG P G PPPPPP GG P PQ
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQ 798
Score = 30.7 bits (66), Expect = 0.26
Identities = 14/41 (34%), Positives = 14/41 (34%)
Frame = +3
Query: 573 PPPPXFXXXPPPPXXXPXGGGGGXXXXPPPPPXTXKXXGGG 695
PPPP P P P PPPPP G G
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAG 774
Score = 29.9 bits (64), Expect = 0.45
Identities = 16/45 (35%), Positives = 16/45 (35%)
Frame = +1
Query: 472 PPPPPPXXKKKXGGXXXXPPPPQXXFFFXGGGXXPPPXXFXXPPP 606
PPPPPP G PPPP GG P P P
Sbjct: 762 PPPPPPPG---VAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEPEP 803
Score = 29.5 bits (63), Expect = 0.60
Identities = 17/63 (26%), Positives = 17/63 (26%)
Frame = -3
Query: 697 PPPPXXFXVXGGGGGXXXXPPPPPXGXXXGGGGXXKKXGGGGXXPPPXKKXXFGGGGGXX 518
PPPP V PPP P G G PPP GG
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRY 792
Query: 517 XXP 509
P
Sbjct: 793 YAP 795
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 33.1 bits (72), Expect = 0.048
Identities = 18/54 (33%), Positives = 20/54 (37%), Gaps = 8/54 (14%)
Frame = +1
Query: 472 PPPPPPXXKKKXG--------GXXXXPPPPQXXFFFXGGGXXPPPXXFXXPPPP 609
PPPPPP ++ G PPPP G PP PPPP
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPP 364
Score = 33.1 bits (72), Expect = 0.048
Identities = 22/75 (29%), Positives = 22/75 (29%)
Frame = +1
Query: 445 GGGXKKXXXPPPPPPXXKKKXGGXXXXPPPPQXXFFFXGGGXXPPPXXFXXPPPPXXXXX 624
G G PPPPPP G P PPQ G PPP P
Sbjct: 328 GNGSSNSSLPPPPPPPRSNAAGS---IPLPPQ------GRSAPPPPPPRSAPSTGRQPPP 378
Query: 625 XGGGGGXXXXPPPPP 669
P PPP
Sbjct: 379 LSSSRAVSNPPAPPP 393
Score = 30.3 bits (65), Expect = 0.34
Identities = 14/36 (38%), Positives = 15/36 (41%), Gaps = 4/36 (11%)
Frame = +2
Query: 599 PPPPXXX----PXXGGGGXXXXPPPPPPXPKXXGGG 694
PPPP P G G PPPPP P+ G
Sbjct: 314 PPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAG 349
Score = 28.3 bits (60), Expect = 1.4
Identities = 16/51 (31%), Positives = 16/51 (31%), Gaps = 4/51 (7%)
Frame = -3
Query: 697 PPPPXXFX----VXGGGGGXXXXPPPPPXGXXXGGGGXXKKXGGGGXXPPP 557
PPPP G G PPPPP G G PPP
Sbjct: 314 PPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPP 364
Score = 26.6 bits (56), Expect = 4.2
Identities = 18/73 (24%), Positives = 20/73 (27%), Gaps = 1/73 (1%)
Frame = +2
Query: 476 PPPPPXXKKKXGXXXXXP-PPPKXXFFXXXXXXXXXXXFFXXPPPPXXXPXXGGGGXXXX 652
P PPP G P PP+ PPP
Sbjct: 251 PAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKR 310
Query: 653 PPPPPPXPKXXGG 691
PPPPPP + G
Sbjct: 311 PPPPPPPSRRNRG 323
Score = 25.8 bits (54), Expect = 7.4
Identities = 14/63 (22%), Positives = 14/63 (22%)
Frame = -3
Query: 697 PPPPXXFXVXGGGGGXXXXPPPPPXGXXXGGGGXXKKXGGGGXXPPPXKKXXFGGGGGXX 518
PPP PPPPP G PPP G
Sbjct: 293 PPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIP 352
Query: 517 XXP 509
P
Sbjct: 353 LPP 355
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 1.4
Identities = 14/31 (45%), Positives = 14/31 (45%)
Frame = +3
Query: 573 PPPPXFXXXPPPPXXXPXGGGGGXXXXPPPP 665
PPPP PPPP P G PPPP
Sbjct: 1707 PPPPPM-SVPPPPSAPPM--PAGPPSAPPPP 1734
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.1 bits (57), Expect = 3.2
Identities = 10/22 (45%), Positives = 11/22 (50%)
Frame = +1
Query: 472 PPPPPPXXKKKXGGXXXXPPPP 537
PPPPPP + PPPP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30
Score = 26.6 bits (56), Expect = 4.2
Identities = 12/24 (50%), Positives = 12/24 (50%)
Frame = +2
Query: 599 PPPPXXXPXXGGGGXXXXPPPPPP 670
PPPP P G PPPPPP
Sbjct: 9 PPPPPPPP---GFEPPSQPPPPPP 29
Score = 25.8 bits (54), Expect = 7.4
Identities = 15/36 (41%), Positives = 15/36 (41%)
Frame = +3
Query: 573 PPPPXFXXXPPPPXXXPXGGGGGXXXXPPPPPXTXK 680
PPPP PPPP P PPPPP K
Sbjct: 9 PPPP-----PPPPGFEPPS-----QPPPPPPPGYVK 34
Score = 22.2 bits (45), Expect(2) = 7.9
Identities = 9/23 (39%), Positives = 10/23 (43%)
Frame = +1
Query: 421 GGXPXFXXGGGXKKXXXPPPPPP 489
G P G + PPPPPP
Sbjct: 7 GNPPPPPPPPGFEPPSQPPPPPP 29
Score = 21.4 bits (43), Expect(2) = 7.9
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = +1
Query: 472 PPPPPPXXKKK 504
PPPPP KK+
Sbjct: 26 PPPPPGYVKKR 36
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 26.6 bits (56), Expect = 4.2
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +2
Query: 473 PPPPPPXXKKKXGXXXXXPPPPK 541
PPPPP PPPPK
Sbjct: 908 PPPPPTASMTASAPAIASPPPPK 930
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 26.6 bits (56), Expect = 4.2
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = +2
Query: 653 PPPPPPXPKXXGGGG 697
PPPPPP P GG
Sbjct: 946 PPPPPPPPLVSAAGG 960
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 25.8 bits (54), Expect = 7.4
Identities = 9/19 (47%), Positives = 9/19 (47%)
Frame = +2
Query: 620 PXXGGGGXXXXPPPPPPXP 676
P G PPPPPP P
Sbjct: 295 PSSGDSANGGLPPPPPPPP 313
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 25.4 bits (53), Expect = 9.7
Identities = 10/25 (40%), Positives = 10/25 (40%)
Frame = +2
Query: 602 PPPXXXPXXGGGGXXXXPPPPPPXP 676
PP P PPPPPP P
Sbjct: 173 PPSAAAPATSLPSDYNPPPPPPPPP 197
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.150 0.512
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,485,006
Number of Sequences: 5004
Number of extensions: 48346
Number of successful extensions: 308
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 42
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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