BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP14_F_C08
(852 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.051
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 31 0.27
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.84
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.5
SPAC26A3.08 |smb1|smb|Sm snRNP core protein Smb1|Schizosaccharom... 28 1.5
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 2.6
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 4.5
SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyc... 27 4.5
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 5.9
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 33.1 bits (72), Expect = 0.051
Identities = 13/30 (43%), Positives = 13/30 (43%)
Frame = +3
Query: 705 PPPPXSXPPQTXPPPPXXXGGXPPXNXPXP 794
PP P P PPPP G PP P P
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 31.1 bits (67), Expect = 0.21
Identities = 15/39 (38%), Positives = 16/39 (41%), Gaps = 3/39 (7%)
Frame = +3
Query: 687 GGXXXXPPPP---XSXPPQTXPPPPXXXGGXPPXNXPXP 794
GG PPPP + PP PPPP G P P
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797
Score = 29.5 bits (63), Expect = 0.63
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = +3
Query: 705 PPPPXSXPPQTXPPPPXXXGGXPPXNXPXP 794
P P PP PPP G PP P P
Sbjct: 754 PAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 28.3 bits (60), Expect = 1.5
Identities = 19/53 (35%), Positives = 19/53 (35%), Gaps = 5/53 (9%)
Frame = +3
Query: 705 PPPPXSXPPQTXP-----PPPXXXGGXPPXNXPXPXXXXXXXXAAHGGXPXPP 848
PPPP P P PPP G PP P P A G P PP
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPG-------VAGAGPPPPP 779
Score = 28.3 bits (60), Expect = 1.5
Identities = 12/30 (40%), Positives = 14/30 (46%), Gaps = 1/30 (3%)
Frame = +3
Query: 339 PPXGXXGGGPPPPXGXXP-XXGGPXXFFXP 425
PP G G GPPPP P G ++ P
Sbjct: 766 PPPGVAGAGPPPPPPPPPAVSAGGSRYYAP 795
Score = 27.5 bits (58), Expect = 2.6
Identities = 14/34 (41%), Positives = 14/34 (41%)
Frame = +1
Query: 337 PPPXGXXGGXPPPXGGXPXXXGXPXXFXPPXNPP 438
PPP GG PPP P G PP PP
Sbjct: 752 PPPAPIMGGPPPP----PPPPGVAGAGPPPPPPP 781
Score = 27.1 bits (57), Expect = 3.4
Identities = 13/39 (33%), Positives = 14/39 (35%)
Frame = +2
Query: 689 GGXXXPPPPXFXXTXNPXPPPXXGGGVTPXKXPXXXPXP 805
GG PPPP P PPP V+ P P
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/35 (37%), Positives = 13/35 (37%)
Frame = +2
Query: 704 PPPPXFXXTXNPXPPPXXGGGVTPXKXPXXXPXPP 808
PPP P PPP G P P P PP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGP---PPPPPPPP 783
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 30.7 bits (66), Expect = 0.27
Identities = 12/25 (48%), Positives = 12/25 (48%)
Frame = +3
Query: 720 SXPPQTXPPPPXXXGGXPPXNXPXP 794
S PP PPPP G PP P P
Sbjct: 3 SLPPGNPPPPPPPPGFEPPSQPPPP 27
Score = 30.7 bits (66), Expect = 0.27
Identities = 11/22 (50%), Positives = 11/22 (50%)
Frame = +3
Query: 687 GGXXXXPPPPXSXPPQTXPPPP 752
G PPPP PP PPPP
Sbjct: 7 GNPPPPPPPPGFEPPSQPPPPP 28
Score = 29.9 bits (64), Expect = 0.48
Identities = 11/25 (44%), Positives = 12/25 (48%)
Frame = +3
Query: 690 GXXXXPPPPXSXPPQTXPPPPXXXG 764
G PPPP P + PPPP G
Sbjct: 7 GNPPPPPPPPGFEPPSQPPPPPPPG 31
Score = 25.8 bits (54), Expect = 7.8
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +2
Query: 311 PPGXXKKKXPPPGGXXGGXPPP 376
PPG PPPG PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPP 26
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.1 bits (62), Expect = 0.84
Identities = 13/29 (44%), Positives = 14/29 (48%), Gaps = 1/29 (3%)
Frame = +3
Query: 705 PPPPXSXPPQ-TXPPPPXXXGGXPPXNXP 788
PPPP S PP + PP P PP P
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 28.7 bits (61), Expect = 1.1
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = +1
Query: 772 PXXTPPXXXXPPXPXXPPTGGXPPHXP 852
P PP PP P PP PP P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAP 1731
Score = 27.9 bits (59), Expect = 1.9
Identities = 10/24 (41%), Positives = 11/24 (45%)
Frame = +1
Query: 772 PXXTPPXXXXPPXPXXPPTGGXPP 843
P PP PP P PP+ PP
Sbjct: 1711 PMSVPPPPSAPPMPAGPPSAPPPP 1734
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/28 (46%), Positives = 13/28 (46%)
Frame = +3
Query: 705 PPPPXSXPPQTXPPPPXXXGGXPPXNXP 788
PP P PP T PP P G PP P
Sbjct: 1208 PPVP---PPSTAPPVPTPSAGLPPVPVP 1232
Score = 27.9 bits (59), Expect = 1.9
Identities = 15/46 (32%), Positives = 16/46 (34%)
Frame = +3
Query: 711 PPXSXPPQTXPPPPXXXGGXPPXNXPXPXXXXXXXXAAHGGXPXPP 848
PP P PP P G PP P P + G P PP
Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPP--VPPPSEAPPVPKPSVGVPPVPP 1212
Score = 25.8 bits (54), Expect = 7.8
Identities = 16/47 (34%), Positives = 16/47 (34%), Gaps = 2/47 (4%)
Frame = +3
Query: 711 PPXSXPPQTXPPPPXXXGGXP--PXNXPXPXXXXXXXXAAHGGXPXP 845
PP P T PP P G P P P P A G P P
Sbjct: 1041 PPVPIPTST-PPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVP 1086
>SPAC26A3.08 |smb1|smb|Sm snRNP core protein
Smb1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 147
Score = 28.3 bits (60), Expect = 1.5
Identities = 15/40 (37%), Positives = 15/40 (37%), Gaps = 2/40 (5%)
Frame = +2
Query: 263 PXXXGXPXXXXPGGXXPP--GXXKKKXPPPGGXXGGXPPP 376
P G P P G P G PPP G G PPP
Sbjct: 103 PAGRGIPLGQAPVGLAGPVRGVGYTAPPPPAGFGRGAPPP 142
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.5 bits (58), Expect = 2.6
Identities = 12/35 (34%), Positives = 12/35 (34%)
Frame = +3
Query: 690 GXXXXPPPPXSXPPQTXPPPPXXXGGXPPXNXPXP 794
G PPPP P T PP N P P
Sbjct: 357 GRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAP 391
Score = 25.8 bits (54), Expect = 7.8
Identities = 10/24 (41%), Positives = 11/24 (45%)
Frame = +3
Query: 705 PPPPXSXPPQTXPPPPXXXGGXPP 776
PPPP S + P PP PP
Sbjct: 340 PPPPRSNAAGSIPLPPQGRSAPPP 363
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.6 bits (56), Expect = 4.5
Identities = 10/28 (35%), Positives = 11/28 (39%)
Frame = +3
Query: 705 PPPPXSXPPQTXPPPPXXXGGXPPXNXP 788
PPPP P+ PP PP P
Sbjct: 1883 PPPPPMALPKAGPPSAAPTSALPPAGPP 1910
>SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 379
Score = 26.6 bits (56), Expect = 4.5
Identities = 17/50 (34%), Positives = 18/50 (36%)
Frame = -1
Query: 435 GVXGGXKXXXXPPXXGXPPXGGGXPPXXPPGGGXFFXXXPGGFXPPGXXF 286
G+ G P G P G G P PGG F GG P G F
Sbjct: 68 GITEGNAAPPPPGAEGGPGAGFGGFPGAGPGGARTFHFNMGG--PGGAQF 115
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 26.2 bits (55), Expect = 5.9
Identities = 19/64 (29%), Positives = 19/64 (29%)
Frame = -1
Query: 435 GVXGGXKXXXXPPXXGXPPXGGGXPPXXPPGGGXFFXXXPGGFXPPGXXFXGXPXFXGXX 256
G GG P G GG GG F PGGF F G P G
Sbjct: 191 GGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGG 250
Query: 255 XXXF 244
F
Sbjct: 251 LGGF 254
Score = 26.2 bits (55), Expect = 5.9
Identities = 22/68 (32%), Positives = 22/68 (32%), Gaps = 1/68 (1%)
Frame = -1
Query: 495 GFFXXFXXXGGIFXVFXKXGGVXGGXKXXXXPPXX-GXPPXGGGXPPXXPPGGGXFFXXX 319
G F F GG GG GG P G P G G GG F
Sbjct: 205 GGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGG 264
Query: 318 PGGFXPPG 295
PGG PG
Sbjct: 265 PGGHGGPG 272
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.315 0.154 0.547
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,818,626
Number of Sequences: 5004
Number of extensions: 29831
Number of successful extensions: 206
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 34
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.8 bits)
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