Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_O18
(894 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 31 0.22
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.39
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.51
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 29 0.67
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 1.2
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 2.1
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 28 2.1
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 2.7
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 2.7
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 4.8
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.8
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 4.8
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 6.3
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 8.3
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 31.1 bits (67), Expect = 0.22
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +3
Query: 720 PXXPPPXPXXXPPXXXPPXPP 782
P PPP P PP PP PP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPP 29
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 30.3 bits (65), Expect = 0.39
Identities = 27/113 (23%), Positives = 47/113 (41%)
Frame = +1
Query: 85 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 264
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 265 SKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIER 423
SKDA A S+ + S + +L N K++ +L+ ++ R
Sbjct: 230 SKDASSNLTACNRSGSSLSSNFYSSRLSKKTSLASLN-KSRASLQHKIMSLSR 281
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 29.9 bits (64), Expect = 0.51
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +1
Query: 298 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 435
KDG+++ +LN FAK L + + +A + + IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 29.5 bits (63), Expect = 0.67
Identities = 13/26 (50%), Positives = 13/26 (50%)
Frame = -3
Query: 805 GGGPXXGXGGXGGXXXGGXXXGXGGG 728
GGG G GG GG G G GGG
Sbjct: 446 GGGSRGGRGGFGGRGGFGGRGGFGGG 471
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +1
Query: 277 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 432
+D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 27.9 bits (59), Expect = 2.1
Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +1
Query: 172 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQQLNAFA 342
++ DFFK + ++ H TL ++ NSL+ +K + G + +LN
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115
Query: 343 KSLQGALGDA 372
+ LQG + A
Sbjct: 116 R-LQGRMNSA 124
>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 27.9 bits (59), Expect = 2.1
Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
Frame = +1
Query: 232 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 393
T++ + SL K D + ++ ES L L L A L D+ KAK
Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411
Query: 394 LEQSRQNIERTAEE 435
LEQ+R +ERT E
Sbjct: 412 LEQARIELERTQRE 425
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.5 bits (58), Expect = 2.7
Identities = 15/41 (36%), Positives = 23/41 (56%)
Frame = +3
Query: 42 LTHSSVFAVPVRISSALSLSTVHHGRQVRSSLRLHRSGPRS 164
++HSS ++P+ SS LS +T+H Q S + S P S
Sbjct: 473 ISHSSASSLPITPSSYLSNTTLHSSVQSSQSSQFTVSVPSS 513
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 27.5 bits (58), Expect = 2.7
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -3
Query: 409 ASTVPKPPWPCRSRLRALPG 350
AST+ K PWP + L +PG
Sbjct: 38 ASTLEKEPWPASTALLVMPG 57
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 4.8
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +3
Query: 720 PXXPPPXPXXXPPXXXPPXPPXP 788
P PPP PP PP P P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGP 1727
Score = 26.2 bits (55), Expect = 6.3
Identities = 13/44 (29%), Positives = 14/44 (31%)
Frame = +1
Query: 730 PPPXPXXXXPXXXPPXPLXPXXAXXPXTXRVXCPPQPXXNTXAA 861
PPP P PP P P A P P P +A
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPGDRSA 1751
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.8
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +1
Query: 178 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 297
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 26.6 bits (56), Expect = 4.8
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +3
Query: 720 PXXPPPXPXXXPPXXXPPXPPXP 788
P PPP P PP PP P
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPP 783
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 6.3
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = +1
Query: 214 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 372
+K+ + E+Q +T DFS K + L+AF K L+ + GDA
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 8.3
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +1
Query: 142 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 318
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 319 LQQL 330
Q+
Sbjct: 73 NNQI 76
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,532,121
Number of Sequences: 5004
Number of extensions: 41110
Number of successful extensions: 198
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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