BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_N18
(857 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 38 0.001
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 35 0.013
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.017
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 34 0.030
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 33 0.052
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 32 0.12
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.21
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 29 1.1
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 28 1.5
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 2.6
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 38.3 bits (85), Expect = 0.001
Identities = 19/51 (37%), Positives = 19/51 (37%)
Frame = +3
Query: 597 PPPPXXFFXXXPPXXXXPXKXXPPPPKXKXXPAPPPXXXFXXXLPPPPXPP 749
PPPP P P PP P P PPP PPPP PP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVP--PPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 36.7 bits (81), Expect = 0.004
Identities = 17/47 (36%), Positives = 18/47 (38%)
Frame = +1
Query: 598 PPPPPXFFXGXPPXXGXPQXXXPPPXKXNXXPPPPPXXXSXXXSPPP 738
PPPPP P P P P PPPPP + PPP
Sbjct: 733 PPPPPAVIVPTPAPAPIP-VPPPAPIMGGPPPPPPPPGVAGAGPPPP 778
Score = 33.1 bits (72), Expect = 0.052
Identities = 18/50 (36%), Positives = 18/50 (36%)
Frame = +1
Query: 556 PPXFIXXXXPXXXXPPPPPXFFXGXPPXXGXPQXXXPPPXKXNXXPPPPP 705
PP I P PPP G PP PPP PPPPP
Sbjct: 736 PPAVIVPTPAPAPIPVPPPAPIMGGPP------PPPPPPGVAGAGPPPPP 779
Score = 29.5 bits (63), Expect = 0.64
Identities = 23/70 (32%), Positives = 23/70 (32%)
Frame = +1
Query: 496 PPPXXPPXFPXPKXGGXXGXPPXFIXXXXPXXXXPPPPPXFFXGXPPXXGXPQXXXPPPX 675
PPP P P PP P PPPP PP G PPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPP------APIMGGPPPP-------PPPPGVAGAGPPPP- 778
Query: 676 KXNXXPPPPP 705
PPPPP
Sbjct: 779 -----PPPPP 783
Score = 29.1 bits (62), Expect = 0.84
Identities = 15/39 (38%), Positives = 15/39 (38%), Gaps = 7/39 (17%)
Frame = +3
Query: 654 KXXPPPPKXKXXPAP-------PPXXXFXXXLPPPPXPP 749
K PPPP P P PP PPPP PP
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768
Score = 26.6 bits (56), Expect = 4.5
Identities = 19/68 (27%), Positives = 20/68 (29%), Gaps = 1/68 (1%)
Frame = +1
Query: 469 PPLXFFFXXPPPXXPPXFPXPKXGGXXGXPPX-FIXXXXPXXXXPPPPPXFFXGXPPXXG 645
PP P P P P GG PP + P PPPP G
Sbjct: 736 PPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAP 795
Query: 646 XPQXXXPP 669
PQ P
Sbjct: 796 APQAEPEP 803
Score = 26.2 bits (55), Expect = 6.0
Identities = 13/36 (36%), Positives = 13/36 (36%)
Frame = +2
Query: 596 PPPPXXXFXXGXPXXXXPXKXXPPPPXKXTPXRPPP 703
PPPP P P PP P P PPP
Sbjct: 732 PPPPPPAVIVPTP-APAPIPVPPPAPIMGGPPPPPP 766
Score = 26.2 bits (55), Expect = 6.0
Identities = 18/55 (32%), Positives = 18/55 (32%)
Frame = +3
Query: 582 PXXXXPPPPXXFFXXXPPXXXXPXKXXPPPPKXKXXPAPPPXXXFXXXLPPPPXP 746
P PPP PP PPPP PPP PPPP P
Sbjct: 746 PAPIPVPPPAPIMGGPPP---------PPPPPGVAGAGPPP--------PPPPPP 783
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 35.1 bits (77), Expect = 0.013
Identities = 33/127 (25%), Positives = 40/127 (31%), Gaps = 8/127 (6%)
Frame = +3
Query: 384 PPXGXKRGXXXXKNPXXPXXPQXXXXQKPPPFXFFFXXPPXXXPLFPXPQKXGXXGXTPX 563
PP K G P P + PPP P P P + G P
Sbjct: 210 PPMHHKPGEHM---PPPPMHHEPGEHMPPPPMHH---EPGEHMPPPPMHHEPGEHMPPPP 263
Query: 564 LYXXXXPXXXXPPPPXXF----FXXXPPXXXXPXKXXPPPP----KXKXXPAPPPXXXFX 719
++ P PPPP PP P + PPPP + P PP
Sbjct: 264 MHHE--PGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPG 321
Query: 720 XXLPPPP 740
+PPPP
Sbjct: 322 EHMPPPP 328
Score = 33.1 bits (72), Expect = 0.052
Identities = 25/89 (28%), Positives = 30/89 (33%), Gaps = 8/89 (8%)
Frame = +3
Query: 498 PPXXXPLFPXPQKXGXXGXTPXLYXXXXPXXXXPPPPXXF----FXXXPPXXXXPXKXXP 665
P P P K G P ++ P PPPP PP P + P
Sbjct: 203 PGEHMPPPPMHHKPGEHMPPPPMHHE--PGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMP 260
Query: 666 PPP----KXKXXPAPPPXXXFXXXLPPPP 740
PPP + P PP +PPPP
Sbjct: 261 PPPMHHEPGEHMPPPPMHHEPGEHMPPPP 289
Score = 27.5 bits (58), Expect = 2.6
Identities = 22/87 (25%), Positives = 27/87 (31%), Gaps = 4/87 (4%)
Frame = +3
Query: 426 PXXPXXPQXXXXQKPPPFXFFFXXPPXXXPLFPXPQKXGXXGXTPXLYXXXXPXXXXPPP 605
P P + PPP P P P + G P ++ P PPP
Sbjct: 260 PPPPMHHEPGEHMPPPPMHH---EPGEHMPPPPMHHEPGEHMPPPPMHHE--PGEHMPPP 314
Query: 606 PXXF----FXXXPPXXXXPXKXXPPPP 674
P PP P + PPPP
Sbjct: 315 PMHHEPGEHMPPPPMHHEPGEHMPPPP 341
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 34.7 bits (76), Expect = 0.017
Identities = 25/76 (32%), Positives = 26/76 (34%), Gaps = 1/76 (1%)
Frame = +1
Query: 514 PXFPXPKXGGXXGXPPXFIXXXXPXXXXPPPPPXFFXGXPPXXGXPQXXXPPPXKXNXXP 693
P P P G PP I PPPPP PP PP + P
Sbjct: 311 PPPPPPPSRRNRGKPP--IGNGSSNSSLPPPPP------PPRSNAAGSIPLPPQGRSAPP 362
Query: 694 PPPP-XXXSXXXSPPP 738
PPPP S PPP
Sbjct: 363 PPPPRSAPSTGRQPPP 378
Score = 29.9 bits (64), Expect = 0.48
Identities = 27/119 (22%), Positives = 30/119 (25%), Gaps = 7/119 (5%)
Frame = +3
Query: 522 PXPQKXGXXGXTPXLYXXXXPXXXXPPPPXXFFXXXPPXXXXPXKXXPPPPKXKXXPA-- 695
P P G P PPPP + PPPP + P+
Sbjct: 314 PPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTG 373
Query: 696 --PPPXXXFXXXLPPPPXPPXTQXXXXXXXXXXXXXXXXXXXP---PPXLXXPPPPXXP 857
PPP PP PP P PP L PP P
Sbjct: 374 RQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLP 432
Score = 27.9 bits (59), Expect = 2.0
Identities = 17/64 (26%), Positives = 17/64 (26%)
Frame = +3
Query: 555 TPXLYXXXXPXXXXPPPPXXFFXXXPPXXXXPXKXXPPPPKXKXXPAPPPXXXFXXXLPP 734
TP P P P P P PP PA PP PP
Sbjct: 419 TPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPP 478
Query: 735 PPXP 746
P P
Sbjct: 479 APAP 482
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 33.9 bits (74), Expect = 0.030
Identities = 16/50 (32%), Positives = 16/50 (32%)
Frame = +3
Query: 597 PPPPXXFFXXXPPXXXXPXKXXPPPPKXKXXPAPPPXXXFXXXLPPPPXP 746
P PP PP P PPP P P LPPP P
Sbjct: 128 PAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQP 177
Score = 28.3 bits (60), Expect = 1.5
Identities = 25/117 (21%), Positives = 29/117 (24%)
Frame = +1
Query: 388 PPGKKGXSXXKKTPXXPXFXKXXXDKNPPLXFFFXXPPPXXPPXFPXPKXGGXXGXPPXF 567
P ++ P P P L PP P P PP
Sbjct: 83 PTSSNNTQQAEERPSMPALGGLFAGGMPKLRHIGKSSASAAPPSAPAPPTPQSELRPPT- 141
Query: 568 IXXXXPXXXXPPPPPXFFXGXPPXXGXPQXXXPPPXKXNXXPPPPPXXXSXXXSPPP 738
P PPP P P PPP + PP S + PP
Sbjct: 142 --SAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPP 196
Score = 27.1 bits (57), Expect = 3.4
Identities = 22/79 (27%), Positives = 22/79 (27%), Gaps = 1/79 (1%)
Frame = +1
Query: 469 PPLXFFFXXPPPXXPPXFPXPKXGGXXGXPPXFIXXXXPXXXXPPPPPXFFXGXPP-XXG 645
PP PP PP P P P PP P PP
Sbjct: 130 PPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPS 189
Query: 646 XPQXXXPPPXKXNXXPPPP 702
P P P K PPPP
Sbjct: 190 LPSAVPPMPPK---VPPPP 205
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 33.1 bits (72), Expect = 0.052
Identities = 19/51 (37%), Positives = 19/51 (37%)
Frame = -1
Query: 734 GGEXXXEXXXGGGGGXXFXFXGGGXXFXGXPXXGGXPXKKXGGGGGXXXXG 582
GGE GG GG F GG F G P G GGG G G
Sbjct: 214 GGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGG 264
Score = 31.9 bits (69), Expect = 0.12
Identities = 19/46 (41%), Positives = 19/46 (41%), Gaps = 1/46 (2%)
Frame = -1
Query: 737 GGGEXXXEXXXGGGGGXXFXFXGGGXXFXGXP-XXGGXPXKKXGGG 603
GGG E GG GG F GG F G P GG P G G
Sbjct: 227 GGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGGPG 272
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.9 bits (69), Expect = 0.12
Identities = 18/61 (29%), Positives = 20/61 (32%)
Frame = +3
Query: 564 LYXXXXPXXXXPPPPXXFFXXXPPXXXXPXKXXPPPPKXKXXPAPPPXXXFXXXLPPPPX 743
L+ P PP PP P PPP P+ PP PPPP
Sbjct: 1677 LFGGMAPAHPVSTPPVRPQSAAPPQMSAPTPP-PPPMSVPPPPSAPPMPAGPPSAPPPPL 1735
Query: 744 P 746
P
Sbjct: 1736 P 1736
Score = 28.7 bits (61), Expect = 1.1
Identities = 17/51 (33%), Positives = 17/51 (33%)
Frame = +1
Query: 583 PXXXXPPPPPXFFXGXPPXXGXPQXXXPPPXKXNXXPPPPPXXXSXXXSPP 735
P P PPP PP P PP PPPP S S P
Sbjct: 1700 PQMSAPTPPPPPMSVPPPPSAPPMPAGPPSA------PPPPLPASSAPSVP 1744
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 31.1 bits (67), Expect = 0.21
Identities = 24/93 (25%), Positives = 25/93 (26%)
Frame = +3
Query: 471 PPFXFFFXXPPXXXPLFPXPQKXGXXGXTPXLYXXXXPXXXXPPPPXXFFXXXPPXXXXP 650
PP PP P P G P P PP P PP
Sbjct: 1131 PPVPVPSGAPPVPKPSVAAPPVPAPSGAPPV----PKPSVAAPPVPAPS-SGIPPVPKPA 1185
Query: 651 XKXXPPPPKXKXXPAPPPXXXFXXXLPPPPXPP 749
P PP + P P P PP PP
Sbjct: 1186 AGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPP 1218
Score = 29.1 bits (62), Expect = 0.84
Identities = 20/81 (24%), Positives = 22/81 (27%)
Frame = +1
Query: 496 PPPXXPPXFPXPKXGGXXGXPPXFIXXXXPXXXXPPPPPXFFXGXPPXXGXPQXXXPPPX 675
PP PP P K P P PP P P P P
Sbjct: 986 PPKDHPPSAPLSKPVSTSPAAPLARVPPVPKLSSKAPPVPLPSADAPPIPVPSTAPPVPI 1045
Query: 676 KXNXXPPPPPXXXSXXXSPPP 738
+ PP P +PPP
Sbjct: 1046 PTS-TPPVPKSSSGAPSAPPP 1065
Score = 28.7 bits (61), Expect = 1.1
Identities = 21/81 (25%), Positives = 22/81 (27%)
Frame = +1
Query: 496 PPPXXPPXFPXPKXGGXXGXPPXFIXXXXPXXXXPPPPPXFFXGXPPXXGXPQXXXPPPX 675
P P P P P P P PP P PP P PP
Sbjct: 1086 PAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVP-VPS-GAPPVP 1143
Query: 676 KXNXXPPPPPXXXSXXXSPPP 738
K + PP P P P
Sbjct: 1144 KPSVAAPPVPAPSGAPPVPKP 1164
Score = 28.7 bits (61), Expect = 1.1
Identities = 25/94 (26%), Positives = 26/94 (27%), Gaps = 4/94 (4%)
Frame = +1
Query: 469 PPLXFFFXXPPPXXP----PXFPXPKXGGXXGXPPXFIXXXXPXXXXPPPPPXFFXGXPP 636
PP+ PP P P P P G P P PP P G PP
Sbjct: 1150 PPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPP 1209
Query: 637 XXGXPQXXXPPPXKXNXXPPPPPXXXSXXXSPPP 738
P P P PP P P P
Sbjct: 1210 VP-PPSTAPPVPTPSAGLPPVPVPTAKAPPVPAP 1242
Score = 28.3 bits (60), Expect = 1.5
Identities = 24/85 (28%), Positives = 26/85 (30%), Gaps = 5/85 (5%)
Frame = +1
Query: 499 PPXXPPXFPXPKXGGXXGXPPX-FIXXXXPXXXXP----PPPPXFFXGXPPXXGXPQXXX 663
PP P P G PP P P PP P G PP P
Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPV--PPPSEA 1197
Query: 664 PPPXKXNXXPPPPPXXXSXXXSPPP 738
PP K + PP P + P P
Sbjct: 1198 PPVPKPSVGVPPVPPPSTAPPVPTP 1222
Score = 27.9 bits (59), Expect = 2.0
Identities = 25/95 (26%), Positives = 27/95 (28%), Gaps = 5/95 (5%)
Frame = +1
Query: 469 PPLXFFFXXPPPXXPPXFPX-PKXG-GXXGXPPXFIXXXXPXXXXPPP---PPXFFXGXP 633
PP+ PP P P PK G PP P P PP
Sbjct: 1032 PPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGI 1091
Query: 634 PXXGXPQXXXPPPXKXNXXPPPPPXXXSXXXSPPP 738
P P PP K + PP P P P
Sbjct: 1092 PPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKP 1126
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 28.7 bits (61), Expect = 1.1
Identities = 19/58 (32%), Positives = 20/58 (34%), Gaps = 1/58 (1%)
Frame = -3
Query: 750 GEEXGGGGXVXGXXXXGGGRXGVXFXG-GGGXXLXGXXXXGXPXKKXXXGGGGXXXGG 580
G G GG G GGGR G G GG G + GG G GG
Sbjct: 13 GSRGGRGGFNGGRGGFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGGRGGAKGG 70
Score = 27.5 bits (58), Expect = 2.6
Identities = 20/57 (35%), Positives = 21/57 (36%)
Frame = -3
Query: 750 GEEXGGGGXVXGXXXXGGGRXGVXFXGGGGXXLXGXXXXGXPXKKXXXGGGGXXXGG 580
G G GG G GGR G F GG G G G + GG G GG
Sbjct: 6 GSRGGRGGSRGGRGGFNGGRGG--FGGGRG----GARGGGRGGARGGRGGRGGARGG 56
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.3 bits (60), Expect = 1.5
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +3
Query: 672 PKXKXXPAPPPXXXFXXXLPPPPXPP 749
P P PPP PPPP PP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.5 bits (58), Expect = 2.6
Identities = 12/39 (30%), Positives = 13/39 (33%)
Frame = +3
Query: 633 PXXXXPXKXXPPPPKXKXXPAPPPXXXFXXXLPPPPXPP 749
P P PPP + A P PPP PP
Sbjct: 156 PTVSAPNSMVSPPPSFQPPSAAAPATSLPSDYNPPPPPP 194
Score = 27.5 bits (58), Expect = 2.6
Identities = 13/34 (38%), Positives = 13/34 (38%)
Frame = +1
Query: 604 PPPXFFXGXPPXXGXPQXXXPPPXKXNXXPPPPP 705
PPP F PP P P PPPPP
Sbjct: 167 PPPSF---QPPSAAAPATSLPSDYNPPPPPPPPP 197
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.145 0.493
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,287,898
Number of Sequences: 5004
Number of extensions: 44857
Number of successful extensions: 231
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 61
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
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