BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_N13
(891 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.17
SPAC343.20 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 31 0.29
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 0.67
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 0.67
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 0.73
SPCC4B3.11c |||conserved eukaryotic protein|Schizosaccharomyces ... 29 0.89
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 29 0.89
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 1.2
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 27 3.6
SPCC16C4.03 |pin1||peptidyl-prolyl cis-trans isomerase Pin1|Schi... 27 3.6
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.6
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 27 3.6
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 4.7
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 4.7
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.5 bits (68), Expect = 0.17
Identities = 15/45 (33%), Positives = 18/45 (40%)
Frame = +1
Query: 595 PXXPPPXPPPPPXXXXXPGPXXPL**GLMGSPPDNIPG*VXPXPP 729
P P PPPPP P P+ G +PP +P P P
Sbjct: 1700 PQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744
>SPAC343.20 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 113
Score = 30.7 bits (66), Expect = 0.29
Identities = 17/49 (34%), Positives = 25/49 (51%)
Frame = -2
Query: 284 TSLTRFLSGMALYSAFSCHRSLLLKLVPTIRSLHCITQTKDKNMKIKXG 138
T+ T+F S Y A + H SLL KL+ L+ + K K ++I G
Sbjct: 58 TNFTKF-SKFVYYLAITLHTSLLTKLIYCHADLYALQSIKYKRLRINNG 105
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.5 bits (63), Expect = 0.67
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +2
Query: 587 SXXPXXPPPPPPPP 628
S P PPPPPPPP
Sbjct: 3 SLPPGNPPPPPPPP 16
Score = 28.3 bits (60), Expect = 1.6
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +1
Query: 853 PPXXPPPPPXPP 888
PP PPPPP PP
Sbjct: 5 PPGNPPPPPPPP 16
Score = 27.9 bits (59), Expect = 2.1
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = +3
Query: 606 PPXPPPPPXXXPXPGAXXPP 665
PP PPPPP P P PP
Sbjct: 9 PPPPPPPPGFEP-PSQPPPP 27
Score = 27.1 bits (57), Expect = 3.6
Identities = 10/21 (47%), Positives = 10/21 (47%)
Frame = +3
Query: 603 PPPXPPPPPXXXPXPGAXXPP 665
PPP PPPP P PP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPP 29
Score = 26.2 bits (55), Expect = 6.3
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +1
Query: 595 PXXPPPXPPPPPXXXXXPGPXXP 663
P PPP PPPP P P
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPP 28
Score = 25.8 bits (54), Expect = 8.3
Identities = 8/10 (80%), Positives = 8/10 (80%)
Frame = +2
Query: 596 PXXPPPPPPP 625
P PPPPPPP
Sbjct: 21 PSQPPPPPPP 30
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 29.5 bits (63), Expect = 0.67
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +3
Query: 606 PPXPPPPPXXXPXPGAXXPPLV 671
P PPPPP P A PPL+
Sbjct: 3 PAPPPPPPAPAPAAAAPAPPLM 24
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.5 bits (58), Expect = 2.7
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = +3
Query: 615 PPPPPXXXPXPGAXXPPL 668
PPPPP P G PPL
Sbjct: 362 PPPPPRSAPSTGRQPPPL 379
Score = 25.8 bits (54), Expect = 8.3
Identities = 10/26 (38%), Positives = 10/26 (38%)
Frame = +1
Query: 814 PPAXKXYXXXXXXPPXXPPPPPXPPP 891
PP PP P PPP P P
Sbjct: 457 PPLPAGMPAAPPLPPAAPAPPPAPAP 482
Score = 23.8 bits (49), Expect(3) = 0.73
Identities = 14/44 (31%), Positives = 18/44 (40%), Gaps = 2/44 (4%)
Frame = +1
Query: 595 PXXPPPXPPP--PPXXXXXPGPXXPL**GLMGSPPDNIPG*VXP 720
P P PP P P P P G + SPP++ P + P
Sbjct: 234 PSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAP 277
Score = 21.4 bits (43), Expect(3) = 0.73
Identities = 8/24 (33%), Positives = 10/24 (41%)
Frame = +1
Query: 814 PPAXKXYXXXXXXPPXXPPPPPXP 885
PP+ + PPPPP P
Sbjct: 294 PPSSRVSAAALAANKKRPPPPPPP 317
Score = 20.6 bits (41), Expect(3) = 0.73
Identities = 6/7 (85%), Positives = 6/7 (85%)
Frame = +1
Query: 868 PPPPXPP 888
PPPP PP
Sbjct: 337 PPPPPPP 343
>SPCC4B3.11c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 116
Score = 29.1 bits (62), Expect = 0.89
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = +1
Query: 286 FFSDPGQQLIMGIIARDLDHSDAEASITAGGIGFSYANIKLKSPRGSGLN 435
F+S PG++ I I+ L S +GG G Y + +KS G N
Sbjct: 27 FYSTPGERRIKDILTEKLSPSSLRVIDVSGGCGSMY-QVAIKSKAFQGKN 75
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 29.1 bits (62), Expect = 0.89
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = +2
Query: 575 TITTSXXPXXPPPPPPPP 628
T P PPPPPPPP
Sbjct: 935 TSVAGVMPAFPPPPPPPP 952
Score = 29.1 bits (62), Expect = 0.89
Identities = 12/26 (46%), Positives = 12/26 (46%)
Frame = +3
Query: 603 PPPXPPPPPXXXPXPGAXXPPLVRVN 680
PPP PPPPP G P V N
Sbjct: 945 PPPPPPPPPLVSAAGGKFVSPAVSNN 970
Score = 28.3 bits (60), Expect = 1.6
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +1
Query: 595 PXXPPPXPPPPP 630
P PPP PPPPP
Sbjct: 942 PAFPPPPPPPPP 953
Score = 28.3 bits (60), Expect = 1.6
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +1
Query: 856 PXXPPPPPXPPP 891
P PPPPP PPP
Sbjct: 942 PAFPPPPPPPPP 953
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 28.7 bits (61), Expect = 1.2
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 2/29 (6%)
Frame = +3
Query: 603 PPPXPPPP--PXXXPXPGAXXPPLVRVNG 683
PPP PPPP P P PP V G
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPAVSAGG 789
Score = 27.5 bits (58), Expect = 2.7
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +1
Query: 604 PPPXPPPPPXXXXXPGPXXP 663
PPP PPPP P P P
Sbjct: 761 PPPPPPPPGVAGAGPPPPPP 780
Score = 26.2 bits (55), Expect = 6.3
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +1
Query: 865 PPPPPXPPP 891
PPPPP PPP
Sbjct: 775 PPPPPPPPP 783
Score = 25.8 bits (54), Expect = 8.3
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +1
Query: 595 PXXPPPXPPPPPXXXXXPGPXXP 663
P P PPPPP GP P
Sbjct: 756 PIMGGPPPPPPPPGVAGAGPPPP 778
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 27.1 bits (57), Expect = 3.6
Identities = 9/20 (45%), Positives = 10/20 (50%)
Frame = +2
Query: 605 PPPPPPPPXXXXXXRGRGSP 664
PPPPPPPP + P
Sbjct: 306 PPPPPPPPPSNDFWKDSNEP 325
Score = 26.2 bits (55), Expect = 6.3
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +3
Query: 603 PPPXPPPPP 629
PPP PPPPP
Sbjct: 306 PPPPPPPPP 314
Score = 26.2 bits (55), Expect = 6.3
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +1
Query: 865 PPPPPXPPP 891
PPPPP PPP
Sbjct: 306 PPPPPPPPP 314
>SPCC16C4.03 |pin1||peptidyl-prolyl cis-trans isomerase
Pin1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 175
Score = 27.1 bits (57), Expect = 3.6
Identities = 19/57 (33%), Positives = 26/57 (45%)
Frame = +2
Query: 230 GKKKPNITPSHSRNESKKYFFRIRDNN*LWVL*PAT*TTRMLKQVLLQEASVSLMPT 400
G KP I SR+ ++ YFF + LW PA LK+ + E S+ PT
Sbjct: 5 GLPKPWIVKI-SRSRNRPYFFNTETHESLWEP-PAATDMAALKKFIANELQESVTPT 59
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.1 bits (57), Expect = 3.6
Identities = 18/45 (40%), Positives = 18/45 (40%)
Frame = -2
Query: 728 GGXGXTHPGMLSGGDPINPH*RGXXGPGXXXXXGGGGGXGGGXXG 594
GG G PG GG G G G GG GG GGG G
Sbjct: 224 GGFGGG-PGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGG 267
Score = 25.8 bits (54), Expect = 8.3
Identities = 13/33 (39%), Positives = 15/33 (45%)
Frame = -2
Query: 692 GGDPINPH*RGXXGPGXXXXXGGGGGXGGGXXG 594
GG+ + G G G GG GG GGG G
Sbjct: 214 GGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGG 246
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 26.6 bits (56), Expect = 4.7
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +2
Query: 605 PPPPPPPP 628
PPPPPPPP
Sbjct: 370 PPPPPPPP 377
Score = 24.2 bits (50), Expect(2) = 3.6
Identities = 8/22 (36%), Positives = 10/22 (45%)
Frame = +2
Query: 608 PPPPPPPXXXXXXRGRGSPFSE 673
PPPPPPP + P +
Sbjct: 370 PPPPPPPPELLNHSPKSRPLGD 391
Score = 21.0 bits (42), Expect(2) = 3.6
Identities = 7/11 (63%), Positives = 7/11 (63%)
Frame = +2
Query: 587 SXXPXXPPPPP 619
S P PPPPP
Sbjct: 367 SYSPPPPPPPP 377
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.6 bits (56), Expect = 4.7
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +2
Query: 605 PPPPPPPP 628
PPPPPPPP
Sbjct: 189 PPPPPPPP 196
Score = 26.6 bits (56), Expect = 4.7
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +2
Query: 605 PPPPPPPP 628
PPPPPPPP
Sbjct: 190 PPPPPPPP 197
Score = 26.2 bits (55), Expect = 6.3
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +1
Query: 865 PPPPPXPPP 891
PPPPP PPP
Sbjct: 189 PPPPPPPPP 197
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.6 bits (56), Expect = 4.7
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +2
Query: 605 PPPPPPPP 628
PPPPPPPP
Sbjct: 1095 PPPPPPPP 1102
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,492,092
Number of Sequences: 5004
Number of extensions: 74040
Number of successful extensions: 580
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 330
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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